In order to investigate the relationship between some environmental factors and the number of artificial inseminations per pregnancy with milk yield, data of 90 healthy Holstein breed dairy cows in a private dairy farm in Karapınar district of Konya province were taken. For the utilization of the data, age, number of artificial inseminations, lactation yield, calving season and sequence of lactation records were taken from the herd management program in the enterprise and analysed. As a result of the analysis, the number of artificial inseminations per pregnancy in 2016 was 1.79 and lactation milk yield was 10079,41 kg; the number of artificial inseminations per pregnancy in 2017 was 2.16 and lactation milk yield was 9767.94 kg. There was a statistically significant relationship between the number of artificial inseminations per pregnancy and milk yield during lactation period in both years. As a result, the increase in the number of artificial inseminations per pregnancy negatively affected the average milk yield of lactation period. Genetic and environmental factors are thought to play a major role in this effect.