The issue of the transport of goods is well-known, yet, in practice, there are often cases of damaged shipments due to improper storage and inappropriately chosen transport technology. Many cases are due to ignorance of the basic characteristics of the cargo and, consequently, its transport characteristics. Vehicle dynamics is crucial to the design of proper cargo securing; therefore, this article provides the values of longitudinal and lateral acceleration of a 16.5 m semi-trailer vehicle combination for test routes of length of 10,827 km on highways and other roads in Slovakia, Austria, and Germany from the monitoring of goods. The horizontal acceleration of 0.2 g is considered as the minimum stability of the load unit that should withstand transport. A load unit with a stability from 0.2 g to 0.3 g could be considered as the weakest load unit. The test results show that even the weakest load units such as these can be damaged in transports, as semi-trailer vehicle combinations still reach longitudinal ax1000 and lateral ay1000 accelerations between 0.2 g and 0.3 g relatively frequently. Acceleration events higher than 0.3 g occur very rarely, at 1.4 event/1000 km for roads, but only 0.1 event/1000 km for highways from our test transports. We have demonstrated through our research that it is necessary for the load units to have a minimum stability of 0.2 g. We can conclude that load units with a stability of less than 0.2 g are completely unacceptable for transport without additional securing because we obtained 70.3 acceleration events per 1000 km in the interval from 0.1 g to 0.2 g on highways but 1148.1 events per 1000 km on other roads. There is a big difference between the number of acceleration events per 1000 km on roads and highways for all acceleration intervals, which means that there is a substantially lower probability of damaging the weak load units on highways than on other roads.