Nucleotidase In Brain Research Articles

A Low‐Km 5′‐Nucleotidase from Rat Brain Cytosolic Fraction: Purification, Kinetic Properties, and Description of Regulation by a Novel Factor that Increases Sensitivity to Inhibition by ATP and ADP

A readily soluble 5'-nucleotidase was purified 1,800-fold from rat brain 105,000-g supernatant. The enzyme showed similarity to the 5'-nucleotidase ectoenzyme of plasma membranes. It exhibited a low Km for AMP, which was preferred over IMP as substrate. It was inhibited by free ATP and ADP and by alpha,beta-methylene ADP. The enzyme appeared to be a glycoprotein on the basis of its interaction with concanavalin A. It contained a phosphatidylinositol moiety because treatment with phosphatidylinositol-specific phospholipase C increased its hydrophilicity. A single subunit of Mr = 54,300 +/- 800 was observed, which is appreciably smaller than published values for the 5'-nucleotidase ectoenzyme or for other low- Km "soluble" 5'-nucleotidases. The soluble 5'-nucleotidase showed an elution profile on AMP-Sepharose affinity chromatography or on Mono Q ion-exchange chromatography different from that of the brain ectoenzyme. Forty-two percent of the soluble 5'-nucleotidase in brain 105,000-g supernatant did not bind to a Mono Q ion-exchange column because of its interaction with a soluble factor. This factor could be removed by chromatography on concanavalin A-Sepharose. The factor had the novel property of increasing the sensitivity of the purified soluble 5'-nucleotidase toward the inhibitor ATP by 20-fold. This factor was also able to increase the inhibition of brain 5'-nucleotidase ectoenzyme by ATP.

Distribution of A1-adenosine receptors, adenosine deaminase and 5′-nucleotidase in brain and other tissues of the pig

Conference Article| August 01 1990 Distribution of A1-adenosine receptors, adenosine deaminase and 5′-nucleotidase in brain and other tissues of the pig VICENT CASADÓ; VICENT CASADÓ 1Departement de Bioquimica i Fisiologia, Unitat de Bioquimica i Biologia Molecular A, Facultat de Quimica, Universitat de Barcelona, Marti i Franques 1, Barcelona 08071, Catalunya, Spain Search for other works by this author on: This Site PubMed Google Scholar TERESA MARTÍ; TERESA MARTÍ 1Departement de Bioquimica i Fisiologia, Unitat de Bioquimica i Biologia Molecular A, Facultat de Quimica, Universitat de Barcelona, Marti i Franques 1, Barcelona 08071, Catalunya, Spain Search for other works by this author on: This Site PubMed Google Scholar JOSEPA MALLOL; JOSEPA MALLOL 1Departement de Bioquimica i Fisiologia, Unitat de Bioquimica i Biologia Molecular A, Facultat de Quimica, Universitat de Barcelona, Marti i Franques 1, Barcelona 08071, Catalunya, Spain Search for other works by this author on: This Site PubMed Google Scholar M. CARMEN LLUIS; M. CARMEN LLUIS 1Departement de Bioquimica i Fisiologia, Unitat de Bioquimica i Biologia Molecular A, Facultat de Quimica, Universitat de Barcelona, Marti i Franques 1, Barcelona 08071, Catalunya, Spain Search for other works by this author on: This Site PubMed Google Scholar ENRIC I. CANELA; ENRIC I. CANELA 1Departement de Bioquimica i Fisiologia, Unitat de Bioquimica i Biologia Molecular A, Facultat de Quimica, Universitat de Barcelona, Marti i Franques 1, Barcelona 08071, Catalunya, Spain Search for other works by this author on: This Site PubMed Google Scholar RAFAEL FRANCO RAFAEL FRANCO * *To whom correspondence should be addressed Search for other works by this author on: This Site PubMed Google Scholar Biochem Soc Trans (1990) 18 (4): 639–641. https://doi.org/10.1042/bst0180639 Article history Received: November 28 1989 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn MailTo Cite Icon Cite Get Permissions Citation VICENT CASADÓ, TERESA MARTÍ, JOSEPA MALLOL, M. CARMEN LLUIS, ENRIC I. CANELA, RAFAEL FRANCO; Distribution of A1-adenosine receptors, adenosine deaminase and 5′-nucleotidase in brain and other tissues of the pig. Biochem Soc Trans 1 August 1990; 18 (4): 639–641. doi: https://doi.org/10.1042/bst0180639 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Society Transactions Search Advanced Search Keywords: R-PIA, R-N6-phenylisopropyladenosine This content is only available as a PDF. © 1990 Biochemical Society1990 Article PDF first page preview Close Modal You do not currently have access to this content.

The quantitative assay of 5'-nucleotidase in brain by histophotometry.

A method for the photometric assay of 5′-nucleotidase in histochemical preparations of brain is described. The method, which is based upon the determination of the specular density under the microscope of the final reaction product, is shown to be valid statistically because of the correlation of the values obtained by it with those obtained by biochemical assay of the enzyme in adjacent sections. Correlation of the specular density with the amount of lead in the final reaction product is also shown. The accuracy of the method of assay is good as judged by a coefficient of variation of 9.1% in a series of replicate measurements. The assay of the amount of enzymic activity by this method shows that it is linearly proportional to the incubation time for periods of up to 2 hr, and also for sections ranging in thickness from 5 to 20 μ.