This study presents numerical simulations and their validation for flow boiling of liquid nitrogen (LN2) in a vertical upflow orientation, with a primary aim to understand the complex two-phase flow and heat transfer phenomena important to space applications. The computational fluid dynamics (CFD) model utilized the coupled level set volume of fluid (CLSVOF) method, incorporating additional source terms for bubble collision dispersion force and shear lift force in the momentum conservation equation to enhance simulation accuracy. The simulations were conducted for two mass velocities (G = 526 and 804 kg/m2s) and three different heat flux levels (approximately 10%, 30%, and 70% of critical heat flux (CHF) under Earth gravity. The model was validated against measured wall temperature data acquired from the authors’ previous experimental studies, demonstrating average deviations of less than 2.8 K across all operating conditions. The simulated two-phase flow contours illustrated various flow patterns, including bubbly, slug, churn, and annular. Both mass velocity and heat flux were observed to impact the onset of nucleate boiling (ONB), bubble nucleation, growth, and coalescence, and overall vapor structure. The simulations also offered insight into axial and radial void fraction and velocity profiles, revealing local flow acceleration trends synchronized with void fraction development. A comparison between predicted and measured bulk fluid temperature profiles showed excellent agreement, further validating the CFD model’s accuracy and practical usefulness for two-phase cryogenic flow boiling simulations in space applications.