Conservation tillage mitigates soil loss in cropland because plant residues help protect the soil, but effects on pesticide movement in surface runoff are not as straightforward. Effects of soil disturbance on surface runoff loss of chlorimuron and alachlor were evaluated utilizing runoff trays. Soil in the trays was either disturbed (tilled) and kept bare or was not tilled, and existing decomposed plant residue was left on the surface. Rainfall (25 mm, 20 min) was simulated 1 d after alachlor (2.8 kg ha −1) or chlorimuron (54 g ha −1) application, and runoff was collected. Runoff fractions were analyzed for herbicide and sediment. Total alachlor loss from bare plots was greater than that in no-tillage plots (4.5% vs. 2.3%, respectively). More than one-third of total alachlor lost from bare plots occurred in the first l of runoff, while no-tillage plots had less runoff volume with a more even distribution of alachlor concentration in the runoff during the rainfall simulation and subsequent runoff period. In contrast, more chlorimuron was lost from no-tillage plots than bare plots (12% vs. 1.5%) even though total runoff volume was lower in the no-tillage plots (10.6 mm vs. 13.6 mm). This was attributed to dense coverage with partially decomposed plant residue in no-tillage plots (1652 kg ha −1) that intercepted chlorimuron. It was likely that chlorimuron, a polar compound, was more easily washed off surface plant residues and transported in runoff.