Background: There are several physiological changes brought on by the inevitable process of aging. For exploring the potential role of aging in male infertility; we examined the semen quality in Northern Sudanese sub fertile men. Objectives: This study aimed to look into possible association between male age and different sperm parameters derived from semen analysis taking into account the World Health Organization (WHO) reference values of semen indicators. Methods: A retrospective descriptive study included 150 Northern Sudanese Couples men presenting with primary or secondary infertility who underwent semen analysis at Modern Specialized Laboratory (MSL), Atbara, River Nile State, Sudan, between August 2021 and April 2023. Seminal fluid were collected in a sterile, wide mouth container, by dry masturbation, after at least 48 hours of abstinence and brought immediately to the laboratory. Color, viscosity, PH and volume were examined macroscopically, and motility under the microscope within 20 minutes to one hour of collection time and was stained by10% Giemsa stain after fixation by spirit for 10-15min. Morphology and abnormal sperms were checked manually to assess the abnormalities in the head, midpiece and the tail of the sperm. This manual method was allowed the discrimination of immature sperm from white blood cells (WBCs) and then counting the WBCs. Data were collected by predesigned closed ended questionnaires, through direct personal interview with patients and questionnaires were filled after an informed consent was taken from each patient. The statistical analysis was done with the help of statistical software for social sciences SPSS 21. The characteristics of the semen, personal and demographic data were analyzed according to the standard protocols. Results: There was significant positive association between age and the different measured parameters except for the reaction and presence of microorganism and, a significant difference was found with the presence of microorganism by p=0.004. Conclusion: Age differences between participants were not observed to have an impact on semen parameters.