Colinus Virginianus Research Articles

Scanning Electron Microscopy of the Oropharyngeal Floor of Northern Bobwhite (Colinus virginianus, Linnaeus, 1758) Focusing on the Numerical and Regional Distribution of the Taste Buds

ABSTRACTThe architecture of the avian oropharynx corresponds to the feeding pattern. The present study aims to provide data on morphology, morphometry, and spatial distribution of taste buds in the oropharyngeal floor of the northern bobwhite (Colinus virginianus) using gross anatomy, morphometric, and scanning electron microscopy. The lower beak tip and tomia revealed the presence of variably‐sized dermal papillae. The prefrenular part of the oral floor had an interrupted median groove. Numerous taste buds and rostral submandibular salivary gland openings were detected alongside this groove. The tongue partially filled the oral floor. Its rostral end formed a median pointed process, the lingual nail. The dorsolateral aspects of the lingual apex and body showed hair‐like and rosette‐shaped filiform papillae of variable densities and sizes. However, these parts were devoid of taste buds. On the other hand, the surface mucosa of the lingual root presented numerous scale‐like cellular elevations and a large number of taste buds. Examination of cross sections in the lingual root suggested the presence of two groups of caudal lingual salivary glands, central and lateral. The laryngeal mound contained taste buds that protruded from the surface epithelium into the lumen. The mucosa of the pharyngeal floor contained many taste buds that appeared similar to those detected on the laryngeal mound, except for the large taste pores, excessive cellular porosity, and denser microvilli. The total proportions of the taste buds in the oral and pharyngeal floors were 34.1% and 65.81%, respectively. The proportions of taste buds were 34.1%, 57.26%, 4.27%, and 4.27% in the sublingual floor, lingual root, laryngeal mound, and pharyngeal wall, respectively. In conclusion, this study provides comprehensive data on the oropharynx of northern bobwhite and will help to understand mechanisms governing food gustation in the studied bird and other related birds.

Northern bobwhite (Colinus virginianus) population response to anthelminthic treatment in the Rolling Plains ecoregion of Texas, 2014–2016

Northern bobwhite quail (Colinus virginianus) are an economically significant gamebird that has experienced continued general decline in the Rolling Plains ecoregion of Texas. Habitat loss and changing environmental conditions have been cited as major contributors to this decline, with factors such as parasites being considered inconsequential. To better assess the impacts of parasite infections on bobwhite populations in the Rolling Plains, bobwhite abundance was monitored in response to anthelminthic treatment. With the prevalence of Oxyspirura petrowi and Aulonocephalus pennula infections in quail from Mitchell County, Texas confirmed by previous studies, the anthelminthic agent Fenbendazole was introduced as a means of parasite control in 2014–2015. Bobwhite abundance was determined through a series of call counts which provided an index of bobwhite populations and were conducted throughout the course of the study. In 2016, call counts revealed a significant increase of bobwhite in the area subject to Fenbendazole treatment, while untreated areas showed no changes in abundance. Fall populations of bobwhite in the treated zone approached 300% of those in untreated areas, these findings suggest that parasites may have a more significant impact on quail populations in the Rolling Plains than previously suspected. With the importance of bobwhite as a game bird in the Rolling Plains, the potential impacts of parasites must be taken into consideration as a factor contributing to bobwhite declines. Further research into the long-term effects these parasites have on quail populations in the ecoregion may aid landowners in developing affordable and effective conservation strategies.

Fine spatial scale assessment of structure and configuration of vegetation cover for northern bobwhites in grazed pastures

BackgroundMonitoring forage in livestock operations is critical to sustainable rangeland management of soil and ecological processes that provide both livestock and wildlife habitat. Traditional ground-based sampling methods have been widely used and provide valuable information; however, they are time-consuming, labor-intensive, and limited in their ability to capture larger extents of the spatial and temporal dynamics of rangeland ecosystems. Drones provide a solution to collect data to larger extents than field-based methods and with higher-resolution than traditional remote sensing platforms. Our objectives were to (1) assess the accuracy of vegetation cover height in grasses using drones, (2) quantify the spatial distribution of vegetation cover height in grazed and non-grazed pastures during the dormant (fall–winter) and growing seasons (spring–summer), and (3) evaluate the spatial distribution of vegetation cover height as a proxy for northern bobwhite (Colinus virginianus) habitat in South Texas. We achieved this by very fine scale drone-derived imagery and using class level landscape metrics to assess vegetation cover height configuration.ResultsEstimated heights from drone imagery had a significant relationship with the field height measurements in September (r2 = 0.83; growing season) and February (r2 = 0.77; dormant season). Growing season pasture maintained residual landscape habitat configuration adequate for bobwhites throughout the fall and winter of 2022–2023 following grazing. Dormant season pasture had an increase in bare ground cover, and a shift from many large patches of tall herbaceous cover (40–120 cm) to few large patches of low herbaceous cover (5–30 cm) (p < 0.05).ConclusionsDrones provided high-resolution imagery that allowed us to assess the spatial and temporal changes of vertical herbaceous vegetation structure in a semi-arid rangeland subject to grazing. This study shows how drone imagery can be beneficial for wildlife conservation and management by providing insights into changes in fine-scale vegetation spatial and temporal heterogeneity from livestock grazing.

Advancing ecotoxicological studies: Utilizing new approach methodologies to enable cross-species extrapolation and reduce avian testing

Ecological risk assessments of agrochemicals have traditionally depended on in vivo guideline tests using northern bobwhite and mallard to provide relevant endpoints for avian species. However, these studies have limitations, including animal welfare concerns, the time and cost involved, limited potential for extrapolation to more realistic exposure conditions, and the lack of mechanistic understanding. The proof-of-concept work presented a case study for thiamethoxam in three avian species, demonstrating the potential of physiologically based kinetic (PBK) modeling to enable dosimetry extrapolations that inform hazard characterization in risk assessment, and reduce the use of avian testing. The model structure for northern bobwhite and mallard contained ten compartments, while an additional ovulation model was included for chicken in the physiological state of egg-laying. The model was first parameterized and evaluated for chicken and northern bobwhite using in vitro kinetic measurements and in vivo toxicokinetic (TK) data. The chicken model was then extrapolated to mallard based on allometric scaling. The models were then used to map the TK profiles across species by simulating internal dose metrics in different avian toxicology studies. These metrics, including peak blood concentrations (Cmax) and area under the curve (AUC) for blood concentration, were determined for acute, subacute, or chronic toxicity endpoints for mallard and northern bobwhite, enabling a quantitative cross-species and cross-route comparison of dosimetry. The results suggested that the chronic toxicological response of birds exposed to thiamethoxam is highly dependent on internal exposure, while mallard appeared to be more dynamically sensitive to thiamethoxam on an acute oral exposure basis. The case study increases the confidence in using new approach methodologies (NAMs) for interpreting avian toxicity studies and facilitating in vitro-in silico-based ecological risk assessments of agrochemicals.

Apparent metabolizable energy and performance of Northern Bobwhite quail fed selected grain sorghum varieties

Apparent metabolizable energy and performance of Northern Bobwhite quail fed selected grain sorghum varieties

Comparing Energetics and Physiological Trait Patterns of North American Birds to Support Ecological Risk Assessment

The environmental risk assessment (ERA) of plant protection products (PPPs) is commonly conducted for a number of standard laboratory species, though its scope includes all species, especially those that are vulnerable or endangered. This begs the question of how the eco-physiological and life history traits of those data-rich species compare to those of less documented species. This paper investigates the utility of a database of such traits, the Add-my-Pet collection of Dynamic Energy Budget theory, for the ERA of North American birds. We identified 72 bird species in the collection that have a federal listing status or are otherwise of interest for the ERA of PPPs and compared their eco-physiological and life history traits in relation to body size, feeding guild, and taxonomic identity to those of 446 terrestrial Holarctic birds, as well as those of standard lab species, notably the northern bobwhite, mallard, and zebra finch. The properties of standard lab species generally align with those of a similar taxonomy and size, with the notable exception of the relatively high reproductive capacity of the northern bobwhite and mallard due to the unique properties of laboratory study data. In addition, taxonomy appears to be a better predictor of eco-physiological traits than feeding guild. We conclude that, to identify representative species for the ERA of PPPs, more traits need to be included, such as those directly linked to the likelihood of exposure and those related to reproductive behavior.

Effects of management practices on Northern Bobwhite Colinus virginianus density in privately owned working forests across the Southeastern United States

Abstract Obtaining rigorous baseline density estimates of species of conservation interest is key when assisting landowners to achieve management goals on private lands. Northern bobwhite (Colinus virginianus) populations are declining throughout their range and despite being the focus of numerous private land conservation initiatives, baseline density estimates in privately owned pine forests are lacking. We sought to address this knowledge gap across the Southeastern United States by sampling 105 privately owned pine stands throughout 2018 to 2020 using observer point count and autonomous recording unit (ARU) sampling data. Using Bayesian hierarchical models, we investigated the influence of stand management (brush management or applied fire) on bobwhite density, as well as four landscape‐scale environmental variables. These included percentage cover of forest, herbaceous, agricultural or burnt land area across six different spatial scales ranging from 500‐m to 10‐km around each pine stand. Baseline density on sites with no management was estimated to be 2.24 coveys per 100 ha (1.00–5.03, 95% BCI), with little impact of applying brush management, but a trend for a positive effect of fire management (0.19, −0.01 to 0.38 95% BCI). This impact of fire was seen at both the stand‐scale, correlated with an increase in acreage of applied prescribed burn management, and across the greater landscape area, correlated with cover of burnt area within a 2‐km buffer around each site. There were also strong positive influences of herbaceous vegetation and a strong negative influence of forest cover on bobwhite density. Practical implication: our sampling efforts fill an important information gap regarding densities throughout private lands in the Southeastern United States. Our study also highlights the necessity of landscape scale planning for Northern Bobwhite conservation initiatives because the efficacy of conservation practices (i.e. prescribed fire and brush management) could be altered by the landscape surrounding the treated forest stand.

EUROPEAN APPROACHES AND REGULATORY MECHANISMS OF ECOLOGICAL AND HYGIENIC MONITORING FROM THE PERSPECTIVE OF POTENTIAL HARMONIZATION AND IMPLEMENTATION INTO DOMESTIC LEGISLATION

Background. The usage of pesticides against pests is also accompanied by potential negative effects on non-target organisms. The risk of harm to biodiversity is quite significant. Implementation of ecological and hygienic monitoring strategies, which is quite effectively applied in the countries of the European Union, will allow to reduce potential threats, financial costs and unforeseen consequences for beneficial insects, birds, aquatic organisms, etc. Aim: analysis of European approaches and regulatory mechanisms of ecological and hygienic monitoring from the standpoint of potential harmonization and implementation in Ukrainian legislation field. Materials and methods. Data from the literature and regulatory documents of the European Union in the field of environmental assessment and ecological and hygienic monitoring were used for the analysis. An array of literary sources was analyzed, which included the most relevant, up-to-date articles over the past 5 years and valid regulatory documents that have the greatest potential for implementation into the national regulatory framework. Results. In the EU and the US, a strict risk assessment process is in place to obtain permission to use pesticides, taking into account the potential impact on non-target organisms. Risk assessment of the use of chemical plant protection on the ecosystem and monitoring of the condition of non-target kinds of objects in the territories of intensive agricultural management is carried out at the stage of pre-registration tests. State regulatory agencies primarily require data on average lethal doses and concentrations for some bird kinds (Colinus virginianus, Anas platrynchos, Perdix perdix, Coturnix japonica). However, there are studies that describe the effects on smaller songbirds that are more sensitive to acute poisoning, therefore, to consider the established standards only for large birds is rather imperfect and needs to be refined in Ukrainian practice in the future. The European rules of environmental risk assessment (ERA) have undergone significant changes in recent decades, which have caused many scientists to worry about insufficient plant protection, slow implementation of scientific developments, simplification of environmental relevance and priority. Also, in many normative documents on pesticides, a political trail is traced, which is sometimes difficult to define and measure. Conclusion. The adoption and implementation of new laws in the field of chemical plant protection in the EU countries are the basis for improving the legislation of Ukraine in this field and ensuring state regulation in the handling of pesticides and agrochemicals.

The effects of habitat, weather, and raptors on northern bobwhite abundance at multiple spatial scales

AbstractRangelands in the southwestern United States represent a current stronghold for northern bobwhite (Colinus virginianus); however, bobwhite populations in rangelands exhibit extreme inter‐annual variability in abundance in relation to fluctuating weather patterns. Recent declining bobwhite population trends within this region have led to the supposition that landscape‐scale processes, such as habitat loss and fragmentation and predation from increased raptor abundance, may be acting in conjunction with weather to reduce bobwhite populations. Our objective was to determine the relative effects of these factors on bobwhite populations in the rangeland environments of Texas and Oklahoma, USA. We obtained publicly available datasets for bobwhite counts (Breeding Bird Survey, state‐agency roadside counts), weather (PRISM), land cover (National Land Cover Database), and raptors (Christmas Bird Counts) for 3 5‐year periods (1990–1994, 1999–2003, 2009–2013). Data were collected at route and landscape scales based on routes within the Rio Grande Plains region of Texas and the Central Mixed Grass Prairie region of Texas and Oklahoma. We used generalized linear mixed models with a backward selection approach to determine top models for each dataset based on scale and ecoregion. Covariate relationships with bobwhite abundance followed expected patterns, with positive relationships with habitat, precipitation, and minimum temperatures and negative relationships with maximum temperatures and raptor abundance. Weather variables were the factors most consistently selected within both regions, while minimum winter temperature was overall the top variable. These relationships occurred within a landscape still containing relatively vast amounts of unfragmented bobwhite habitat (&gt;60% rangeland; &gt;15 million ha). Management within these regions should be focused on retaining habitat at a broad scale, while managing for suitable cover at a local scale to help mitigate weather effects.

Dynamic analysis of a fuzzy Bobwhite quail population model under g-division law

This paper is concerned with a kind of Bobwhite quail population model xn+1=A+Bxn+xnxn-1xn-2,n=0,1,⋯,\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$$\\begin{aligned} x_{n+1}=A+Bx_n+\\frac{x_n}{x_{n-1}x_{n-2}},\\ \\ n=0,1,\\cdots , \\end{aligned}$$\\end{document}where the parameters and initial values are positive parabolic fuzzy numbers. According to g-division of fuzzy sets and based on the symmetrical parabolic fuzzy numbers, the conditional stability of this model is proved. Besides the existence, boundedness and persistence of its unique positive fuzzy solution. When some fuzzy stability conditions are satisfied, the model evolution exhibits oscillations with return to a fixed fuzzy equilibrium no matter what the initial value is. This phenomena provided a vivid counterexample to Allee effect in density-dependent populations of organisms. As a supplement, two numerical examples with data-table are interspersed to illustrate the effectiveness. Our findings have been verified precise with collected northern bobwhite data in Texas, and will help to form some efficient density estimates for wildlife populations of universal applications.

Selection of suitable reference genes for normalization of RT-qPCR in three tissues of Northern bobwhite (Colinus virginianus) infected with eyeworm (Oxyspirura petrowi).

The Northern bobwhite (Colinus virginianus) is an economically important, and popular game bird in North America. Northern bobwhites have experiencing declines of > 3.5% annually in recent decades due to several factors. The eyeworm Oxyspirura petrowi is a nematode parasite frequently found in the eyes of bobwhites. Although reported frequently in wild bobwhites, there is no research to understand the host-parasite mechanism. Hence, it is important to investigate mechanisms of eyeworm invasion and immune modulation in bobwhite. Cytokine gene expression using RT-PCR is widely used to identify the innate immune response of a host to an infection. In this study, we evaluated ten reference genes (HMBS, RPL19, RPL32, RPS7, RPS8, TATA, SDHA, YWHAZ, GAPDH, and ACTB) for their stability across three tissues (liver, spleen, and caecal tonsils) of control and O. petrowi infected Northern bobwhites. Primer efficiency and reference genes stability were assessed using GeNorm, NormFinder, and BestKeeper. Expression of these reference genes with respect to O. petrowi infection in bobwhites showed RPL32 and HMBS were the most stable genes in the liver, HMBS and SDHA were the most stable genes in the spleen, and HMBS and YWHAZ were equally stable reference genes in the caecal tonsils. Based on the geometric mean of all three analyses, our results indicate that the combination of RPL32 and HMBS for the liver, HMBS and SDHA for the spleen, and YWHAZ and HMBS for caecal tonsils might be used as reference genes for normalization in gene expression investigations on Northern bobwhites.

Surveillance for Selected Pathogens and Parasites of Northern Bobwhite (Colinus virginianus) from Western Oklahoma, USA, 2018-20.

The Northern Bobwhite (Colinus virginianus) has been undergoing a range-wide population decline. Potential causes for declines across its historic range have been investigated for decades and include habitat loss and fragmentation and a variety of parasitic and infectious diseases. Although there have been studies on bobwhite ecology in Oklahoma, USA, relatively little is known about parasites and pathogens in the region. We evaluated the health of free-ranging bobwhites from nine sites in western Oklahoma. From 2018 to 2020, 206 bobwhites were evaluated for gross and microscopic lesions and tested for selected pathogens. In general, bobwhites were in good nutritional condition with ample muscle mass and fat stores. No significant gross lesions were observed in any bobwhite and no significant histologic lesions were detected in a subset. There was no evidence of infection with or exposure to reticuloendotheliosis virus, West Nile virus, respiratory Mycoplasmataceae species, Pasteurella multocida, intestinal Eimeria spp., or oral Trichomonas spp. Several pathogens of potential concern were detected, including avian adenovirus (8.6%), Toxoplasma gondii (2.3%), and haemosporidians (a Haemoproteus sp. (1.5%), Leucocytozoon schoutedeni (1.5%), and Plasmodium homopolare haplotype 2 [lineage LAIRI01; 3.6%]). Physaloptera sp. (12%) and Sarcocystis sp. (1%) were detected in the breast muscle. Low intraspecific genetic diversity was noted for Physaloptera sp., and sequences were most similar to Physaloptera sequences from bobwhites and grasshoppers (Orthoptera) in Texas. Low intensities of chewing lice, chiggers, and ticks were observed. A subset of bobwhites had evidence of exposure to selected toxicants and heavy metals; a small number had low levels of iron, manganese, zinc, molybdenum, and copper, which were not considered diagnostically relevant. In general, bobwhites from western Oklahoma appeared to be in good health with a low diversity of pathogens detected, but future work is needed to understand potentially changing disease risks for this population.

Evaluating the Use of a Thermal Sensor to Detect Small Ground-Nesting Birds in Semi-Arid Environments during Winter

Aerial wildlife surveys with fixed-wing airplanes and helicopters are used more often than on-the-ground field surveys to cover areas that are both extensive and often inaccessible. Drones with high-resolution thermal sensors are being widely accepted as research tools to aid in monitoring wildlife species and their habitats. Therefore, our goal was to assess the feasibility of detecting northern bobwhite quail (Colinus virginianus, hereafter ‘bobwhite’) using drones with a high-resolution thermal sensor. Our objectives were (1) to identify the altitudes at which bobwhites can be detected and (2) compare the two most used color palettes to detect species (black-hot and isotherm). We achieved this goal by performing drone flights at different altitudes over caged tame bobwhites and capturing still images and video recordings at altitudes from 18 to 42 m. We did not observe or detect any obvious signs of distress, movement, or fluttering of bobwhites inside cages caused by the noise or presence of the drone during data acquisition. We observed the highest counts of individual bobwhites with the black-hot thermal palette at 18 m (92%; x¯ = 47 bobwhites; SE = 0.41) and at 24 m (81%; x¯ = 41 bobwhites; SE = 0.89). The isotherm thermal palette had lower count proportions. The use of video to count quail was not feasible due to the low resolution of the video and the species size. Flying drones with high-resolution thermal sensors provided reliable imagery to detect roosting bobwhite individuals in South Texas during the winter.

Divergent island hybrids mixed waves of ancient gene flow.

In this issue of Molecular Ecology, Salter etal.(2023) discovered that the Cuban Northern Bobwhite subspecies, Colinus virginianus cubanensis (Gould,1850), is an ancient hybrid population formed due to historical hybridization potentially brought by waves of historical human migration. This study revealed a complex mixture of gene flow from distinct spatiotemporal origins underlying a seemingly semi-independent evolutionary trajectory. Hybridization can be more common and complex than we thought.

IMPLICATIONS OF TREATING PARASITIC INFECTION IN NORTHERN BOBWHITE (COLINUS VIRGINIANUS) ON OVERALL HELMINTH LIFE CYCLE.

This study was conducted to determine how reducing the parasite burden in a definitive host may affect the prevalence in intermediate hosts. Here we used the eyeworm Oxyspirura petrowi and cecal worm Aulonocephalus pennula as model species. Northern bobwhite quail (Colinus virginianus) were provided an anthelmintic medicated feed in wild systems because of convincing evidence that these parasites were suppressing their populations. Eyeworm and cecal worm prevalence were measured in Orthopterans, which act as intermediate hosts, using polymerase chain reaction. Individuals were collected from a control site, a site treated for 2 yr, and a site treated for 5 yr. Orthopteran community composition was significantly different among the sites; however, an interaction between subfamily and site was not significant for the eyeworm. There was a significant reduction in eyeworm-infected Orthopterans on the 5-yr site compared with the other 2, suggesting that treatment of a definitive host may indeed affect the prevalence of eyeworms during other life-cycle stages. There was an interaction between the Orthopteran subfamily and the site for the cecal worm, so results were analyzed within each subfamily. A significant reduction in the prevalence of cecal worms was only found in the Cyrtacanthacridinae subfamily on the 5-yr site when compared with the other sites. However, the greatest prevalence in the Gomphocerinae and Oedipodinae subfamilies across all 3 sites was 4.1%. This indicates an unknown degree of cecal worm host specificity. Therefore, conclusions could not be made through the simple assessment of prevalence.

Using drones equipped with thermal cameras to locate and count quail individuals and coveys: A case study using Northern Bobwhite Colinus virginianus in Mississippi, USA

Abstract The presence and abundance of Northern Bobwhite Colinus virginianus often relies on imprecise methods (e.g. fall covey counts), which can lead to inaccurate estimates resulting in poor management practices. Drones equipped with thermal sensors have proven beneficial for monitoring wildlife but still require sufficient field validation regarding precision. We tested the application of thermal drones for locating and counting Northern Bobwhite in Mississippi, USA. We conducted flights over known locations of Northern Bobwhite representing three individual, radio‐marked birds during the breeding season and seven covey locations during the wintering season shortly after dusk. Individuals and coveys were located in 67% and 86% of flights, respectively. In some scenarios, bobwhite could be seen from altitudes of 120 m because heat signatures of multiple individuals grouped together in the covey provided an easily recognisable feature, in contrast to individual birds alone. Counts were accurate for two coveys and underestimated remaining coveys, because individual birds were sometimes visually obstructed from above. The small body size of bobwhite results in few pixels in thermal images and depends on ground sampling distance as a function of flight altitude. Our flights were tested at known vicinities of bobwhite locations, yet our method shows promise for locating unknown coveys as well. Future systematic methods may benefit from automated image recognition using computer vision and machine learning to improve count accuracy and save time, costs and labor.

Validation of an enzyme immunoassay for the detection of corticosterone metabolites from northern bobwhite (Colinus virginianus) feces.

Interest in the effects of stressors on wildlife has grown substantially over the past few decades. As this interest has grown, so has the need for minimally invasive and reliable methods for estimating differences in the levels of stress hormones. An enzyme immunoassay using standardized methods was validated for detecting concentrations of corticosterone (cort) metabolites from northern bobwhite fecal samples. Two physiological challenges and one biological challenge were applied to 18 northern bobwhites (nine males and nine females), and the fecal cort metabolite concentrations were compared to baseline levels. The interactions of sex and treatment, treatment and time and sex and time were all significant. Thus, the methods and tools used here were sensitive enough to detect expected changes to the hypothalamo-pituitary-adrenal axis of northern bobwhite.

Detection of Cryptosporidium‎‎ spp in Bobwhite Quails (Coturnix coturnix) by ‎Traditional Methods in Baghdad City, Iraq

Cryptosporidiosis is one of the most important zoonosis diseases distributed ‎worldwide causes severe diarrhea in animals and is lethal in quails. This study detected ‎Cryptosporidium‎‎ spp. in quails in Baghdad province Iraq by flotation and staining methods ‎and compared these results with PCR results from the previous study. This research was ‎carried out from January 2022 to September 2022. Out of 180 quails (Coturnix coturnix) in ‎total were investigated. The overall infection rate of Cryptosporidium‎‎ spp. in quails was ‎‎23.9% (43/180). Young quail had a greater infection rate of 44.2% (19/43) while adult ‎quails had a lower infection rate of 17.2% (24/137). Finding from the research revealed that there were ‎no significant differences between males and females and highly significant differences ‎among the months of study with the highest infection rate being 45% (9/20) in April, and ‎the lowest infection rate being 10% in June and July. This disease was presented in birds ‎and birds act as a mechanical carrier of Cryptosporidium‎‎ spp. between a conventional acid-‎fast staining procedure and PCR test, PCR can not only detect Cryptosporidium‎‎ but is also ‎able to differentiate between what appear to be host-adapted genotypes of the parasite with ‎high sensitivity and specificity‎‎‎.

Quail Farming in Villages of Mogoditshane-Thamaga and Tlokweng Districts, Botswana

A The interest in quail farming has been increasing over the years due to the quail’s many productive and financial benefits over other poultry species. Quail farming is still in its infancy in Botswana. This study investigated the current status, challenges, and prospects of Japanese quail farming in Mogoditshane, Gabane, and Tlokweng districts, Botswana. A total of 64 quail farmers were interviewed using a structured questionnaire from June 2022 to April 2023. Data were collected on the respondents’ demographic characteristics (age, educational status, and sex), quail management aspects (feeding, housing, and health), ownership of quail, challenges in quail farming, and the use of quail products in the study area. Results showed that 67% of male respondents were involved in quail rearing. The youth (≤35 years) dominated the rearing of quails, followed by respondents aged 41-50 years (22%), 36-40 years (16%), and above 50 years (9%). In addition, 48% of the respondents reared ≤100 quails, followed by 39% and 13% who reared 101-500 and 500 quails, respectively. Furthermore, 81% of respondents reared quails in cages, 17% in conventional structures, and 2% in residential houses. Bobwhite, Jumbo, and Japanese quail were the three quail varieties reared in the study area. It was found that 55% of the respondents had less than one year of experience in quail farming. Moreover, 86% of the respondents used crushed maize or sorghum to feed quails, while 14% used commercial chicken diets. Finally, 92% of the respondents mentioned that quail eggs were used to treat various human diseases. Effective challenges in quail farming included external parasites (36%), diseases (30%), predation (13%), lack of commercial quail diets (12%), escaping (6%), and theft (3%). Quail farming should be considered for inclusion in government support programs as it has the potential to contribute to income generation and food and nutrition security.

Reproductive toxicity in birds predicted by physiologically-based kinetics and bioenergetics modelling

Effects on the growth and reproduction of birds are important endpoints in the environmental risk assessment (ERA) of pesticides. Toxicokinetic-toxicodynamic models based on dynamic energy budget theory (DEB) are promising tools to predict these effects mechanistically and make extrapolations relevant to ERA. However, before DEB-TKTD models are accepted as part of ERA for birds, ecotoxicological case studies are required so that stakeholders can assess their capabilities.We present such a case-study, modelling the effects of the fluopyram metabolite benzamide on the northern bobwhite quail (Colinus virginianus). We parametrised a DEB-TKTD model for the embryo stage on the basis of an egg injection study, designed to provide data for model development. We found that information on various endpoints, such as survival, growth, and yolk utilisation were needed to clearly distinguish between the performance of model variants with different TKTD assumptions. The calibration data were best explained when it was assumed that chemical uptake occurs via the yolk and that benzamide places stress on energy assimilation and mobilisation.To be able to bridge from the in vitro tests to real-life exposure, we developed a physiologically-based toxicokinetic (PBK) model for the quail and used it to predict benzamide exposure inside the eggs based on dietary exposure in a standard reproductive toxicity study. We then combined the standard DEB model with the TKTD module calibrated to the egg injection studies and used it to predict effects on hatchling and 14-day chick weight based on the exposure predicted by the PBK model. Observed weight reductions, relative to controls, were accurately predicted. Thus, we demonstrate that DEB-TKTD models, in combination with suitable experimental data and, if necessary, with an exposure model, can be used in bird ERA to predict chemical effects on reproduction.