This study analyzed 14 stratigraphic sections exposing the Devonian–Carboniferous boundary interval in the North Urals, representing different facies from shallow-water carbonate platform to bathyal, in order to assess the possibility of recognizing regional levels corresponding to the base of the conodont zones S. sulcata and Protognathodus kockeli. The base of the S. sulcata Zone is regionally recognized by the first appearances of the conodonts Siphonodella sulcata (Huddle), S. semichatovae Kononova and Lipnjagov and Patrognathus crassus Kononova and Migdisova, roughly correlated with the first appearance of the foraminifer Tournayellina pseudobeata Reitlinger and Kulagina, and with the first appearances of the ostracods Cribroconcha primaris Kochetkova, Chamishaella grekoffi Tchizhova, Glyptolichvinella spiralis (Jones and Kirkby), Armilla uralica Sobolev, Calcarofera ex gr. media Schornikov, Compositocostata cumina Sobolev, Spinoalacia tschigovae Sobolev, Richterina latior Rabien. The base of the S. sulcata Zone occurs in the interval between two positive inorganic carbon isotope excursions. The base of the Protognathodus kockeli Zone is marked by the first appearance of Protognathodus kockeli (Bischoff) in a very narrow facies belt on the slope of the carbonate platform, recognized regionally by the onset of the last phase of the positive inorganic carbon isotope excursion, the beginning of the transgression after the terminal Famennian regression, the beginning of the unilocular foraminifera Bisphaera bloom and probably the appearance of the ostracods Monoceratina sp. 1. The level corresponding to the base of Pr. kockeli Zone is poorly supported biostratigraphically, but is characterized by clear isotope-stratigraphic and cyclostratigraphic data, although the eustatic signal may be obscured in places by local tectonics.