Abstract We assess the representation of mesoscale stirring in a suite of models against an estimate derived from microstructure data collected during the North Atlantic Tracer Release Experiment (NATRE). We draw heavily from the approximate temperature variance budget framework of Ferrari and Polzin. This framework assumes two sources of temperature variance away from boundaries: first, the vertical stirring of the large-scale mean vertical gradient by small-scale turbulence; and second, the lateral stirring of large-scale mean along-isopycnal gradients by mesoscale eddies. Temperature variance so produced is transformed and on average transferred down scales for ultimate dissipation at the microscale at a rate χ estimated using microstructure observations. Ocean models represent these pathways by a vertical mixing parameterization, and an along-isopycnal lateral mixing parameterization (if needed). We assess the rate of variance production by the latter as a residual from the NATRE dataset and compare against the parameterized representations in a suite of model simulations. We find that variance production due to lateral stirring in a Parallel Ocean Program version 2 (POP2) 1/10° simulation agrees well, to within the estimated error bars, with that inferred from the NATRE estimate. A POP2 1° simulation and the Estimating the Circulation and Climate of the Ocean Version 4 release 4 (ECCOV4r4) simulation appear to dissipate an order of magnitude too much variance by applying a lateral diffusivity, when compared to the NATRE estimate, particularly below 1250 m. The ECCOV4r4-adjusted lateral diffusivities are elevated where the microstructure suggests elevated χ sourced from mesoscale stirring. Such elevated values are absent in other diffusivity estimates suggesting the possibility of compensating errors and caution in interpreting ECCOV4r4’s adjusted lateral diffusivities. Significance Statement We look at whether microstructure turbulence observations can provide a useful metric for judging the fidelity of representation of mesoscale stirring in a suite of models. We focus on the region of the North Atlantic Tracer Release Experiment (NATRE), the site of a major ocean turbulence observation campaign, and use an approximate variance budget framework for the region with observational estimates from Ferrari and Polzin (2005). The approach provides a novel framework to evaluate the approximate representation of mesoscale stirring in a variety of models.