Normalized Green Red Difference Index Research Articles

High-Throughput Phenotyping for the Evaluation of Agronomic Potential and Root Quality in Tropical Carrot Using RGB Sensors

The objective of this study was to verify the genetic dissimilarity and validate image phenotyping using RGB (red, green, and blue) sensors in tropical carrot germplasms. The experiment was conducted in the city of Carandaí-MG, Brazil, using 57 tropical carrot entries from Seminis and three commercial entries. The entries were evaluated agronomically and two flights with Remotely Piloted Aircraft (RPA) were conducted. Clustering was performed to validate the existence of genetic variability among the entries using an artificial neural network to produce a Kohonen’s self-organizing map. The genotype–ideotype distance index was used to verify the best entries. Genetic variability among the tropical carrot entries was evidenced by the formation of six groups. The Brightness Index (BI), Primary Colors Hue Index (HI), Overall Hue Index (HUE), Normalized Green Red Difference Index (NGRDI), Soil Color Index (SCI), and Visible Atmospherically Resistant Index (VARI), as well as the calculated areas of marketable, unmarketable, and total roots, were correlated with agronomic characters, including leaf blight severity and root yield. This indicates that tropical carrot materials can be indirectly evaluated via remote sensing. Ten entries were selected using the genotype–ideotype distance (2, 15, 16, 22, 34, 37, 39, 51, 52, and 53), confirming the superiority of the entries.

Deciphering temporal growth patterns in maize: integrative modeling of phenotype dynamics and underlying genomic variations.

Quantifying the temporal or longitudinal growth dynamics of crops in diverse environmental conditions is crucial for understanding plant development, requiring further modeling techniques. In this study, we analyzed the growth patterns of two different maize (Zea mays L.) populations using high-throughput phenotyping with a maize population consisting of 515 recombinant inbred lines (RILs) grown in Texas and a hybrid population containing 1090 hybrids grown in Missouri. Two models, Gaussian peak and functional principal component analysis (FPCA), were employed to study the Normalized Green-Red Difference Index (NGRDI) scores. The Gaussian peak model showed strong correlations (c. 0.94 for RILs and c. 0.97 for hybrids) between modeled and non-modeled temporal trajectories. Functional principal component analysis differentiated NGRDI trajectories in RILs under different conditions, capturing substantial variability (75%, 20%, and 5% for RILs; 88% and 12% for hybrids). By comparing these models with conventional BLUP values, common quantitative trait loci (QTLs) were identified, containing candidate genes of brd1, pin11, zcn8 and rap2. The harmony between these loci's additive effects and growing degree days, as well as the differentiation of RIL haplotypes across growth stages, underscores the significant interplay of these loci in driving plant development. These findings contribute to advancing understanding of plant-environment interactions and have implications for crop improvement strategies.

Using UAV multispectral photography to discriminate plant species in a seep wetland of the Fynbos Biome

Wetlands harbour a wide range of vital ecosystems. Hence, mapping wetlands is essential to conserving the ecosystems that depend on them. However, the physical nature of wetlands makes fieldwork difficult and potentially erroneous. This study used multispectral UAV aerial photography to map ten wetland plant species in the Fynbos Biome in the Steenbras Nature Reserve. We developed a methodology that used K-Nearest Neighbour (KNN), Support Vector Machine (SVM), and Random Forest (RF) machine learning algorithms to classify ten wetland plant species using the preselected bands and spectral indices. The study identified Normalized green red difference index (NGRDI), Red Green (RG) index, Green, Log Red Edge (LogRE), Normalized Difference Red-Edge (NDRE), Chlorophyll Index Red-Edge (CIRE), Green Ratio Vegetation Index (GRVI), Normalized Difference Water Index (NDWI), Green Normalized Difference Vegetation Index (GNDVI) and Red as pertinent bands and indices for classifying wetland plant species in the Proteaceae, Iridaceae, Restionaceae, Ericaceae, Asteraceae and Cyperaceae families. The classification had an overall accuracy of 87.4% and kappa accuracy of 0.85. Thus, the findings are pertinent to understanding the spectral characteristics of these endemic species. The study demonstrates the potential for UAV-based remote sensing of these endemic species.

Monitoring leaf area index of the sown mixture pasture through UAV multispectral image and texture characteristics

Leaf area index (LAI) is an important phenotypic trait closely related to photosynthesis, respiration, and water utilization. In recent years, unmanned aerial vehicles (UAVs) multispectral capabilities enable the acquisition of spectral information from visible light to near infrared, facilitating vegetation growth monitoring. This study aims to explore the methodology of combining vegetation indices, color indices, texture information, and ecological factors based on UAV multispectral images to enhance the accuracy of the sown mixture pasture LAI estimation. A field experiment involving 13 mixed sowing combinations of alfalfa (Medicago sativa L.), tall fescue (Festuca elata Keng ex E. Alexeev) and plantain (Plantaga lanceolata L.) was conducted out. Multiple linear regression, Bagging algorithm, support vector machine (SVM), random forest algorithm (RF), KNN algorithm, and back propagation neural network (BP) were used to construct the LAI prediction model. The results showed that combining vegetation index (VI) + color index (CI) + normalized difference texture index (NDTI), and ecological factors (EF) could enhance the accuracy of LAI estimation. The sensitive characteristic combinations for alfalfa, tall fescue, and plantain were found to be NDRE (Normalized difference red-edge index) + NGBDI (Normalized green–blue difference index) + (B) MEA-(G) HOM (Blue band Mean - Green band Homogeneity) + DTR (Daily temperature difference), MSR (Modified simple ratio) + NGRDI (Normalized green–red difference index) + (G) COR-(R) VAR (Green band Correlation – Red band Variance) + DTR (Daily temperature difference)), and MSR (Modified simple ratio) + ExG (Excess green) + (G) SEM-(G) MEA (Green band Second-order moment – Green band Mean) + DTR (Daily temperature difference), respectively. RF exhibited superior prediction capability, further enhancing the accuracy of forage LAI prediction. The alfalfa, tall fescue, and plantain obtained coefficient of determination (R2) of 0.83, 0.79 and 0.79, root mean squared error (RMSE) of 0.50, 0.58 and 0.70, and mean absolute error (MAE) of 0.36, 0.45 and 0.55, respectively. These findings provide valuable insights for the estimation of leaf area index of the sown mixture pasture through UAV multispectral images and texture characteristics.

RGB image-based method for phenotyping rust disease progress in pea leaves using R

BackgroundRust is a damaging disease affecting vital crops, including pea, and identifying highly resistant genotypes remains a challenge. Accurate measurement of infection levels in large germplasm collections is crucial for finding new resistance sources. Current evaluation methods rely on visual estimation of disease severity and infection type under field or controlled conditions. While they identify some resistance sources, they are error-prone and time-consuming. An image analysis system proves useful, providing an easy-to-use and affordable way to quickly count and measure rust-induced pustules on pea samples. This study aimed to develop an automated image analysis pipeline for accurately calculating rust disease progression parameters under controlled conditions, ensuring reliable data collection.ResultsA highly efficient and automatic image-based method for assessing rust disease in pea leaves was developed using R. The method’s optimization and validation involved testing different segmentation indices and image resolutions on 600 pea leaflets with rust symptoms. The approach allows automatic estimation of parameters like pustule number, pustule size, leaf area, and percentage of pustule coverage. It reconstructs time series data for each leaf and integrates daily estimates into disease progression parameters, including latency period and area under the disease progression curve. Significant variation in disease responses was observed between genotypes using both visual ratings and image-based analysis. Among assessed segmentation indices, the Normalized Green Red Difference Index (NGRDI) proved fastest, analysing 600 leaflets at 60% resolution in 62 s with parallel processing. Lin’s concordance correlation coefficient between image-based and visual pustule counting showed over 0.98 accuracy at full resolution. While lower resolution slightly reduced accuracy, differences were statistically insignificant for most disease progression parameters, significantly reducing processing time and storage space. NGRDI was optimal at all time points, providing highly accurate estimations with minimal accumulated error.ConclusionsA new image-based method for monitoring pea rust disease in detached leaves, using RGB spectral indices segmentation and pixel value thresholding, improves resolution and precision. It rapidly analyses hundreds of images with accuracy comparable to visual methods and higher than other image-based approaches. This method evaluates rust progression in pea, eliminating rater-induced errors from traditional methods. Implementing this approach to evaluate large germplasm collections will improve our understanding of plant-pathogen interactions and aid future breeding for novel pea cultivars with increased rust resistance.

PEMANFAATAN NORMALIZED GREEN RED DIFFERENCE INDEX (NGRDI) UNTUK MENDUGA KADAR N-TOTAL PADA LAHAN PADI DI KABUPATEN PAMEKASAN

Inefficient fertilization is the cause of decreased rice production. This is due to the inappropriate use of fertilizers. The absence of information on soil nitrogen content in their area causes farmers not to apply fertilizer properly. Analysis of soil nitrogen content in Pamekasan Regency requires much time, cost, and effort. Remote sensing methods can be used to obtain information on soil nitrogen levels by reducing the number of samples used. This study aims to estimate nitrogen content in rice fields using the vegetation index in the form of NGRDI (Normalized Green Red Difference Index). This research was conducted by purposive random sampling method based on 40 land map units formed in Pamekasan Regency, laboratory analysis, statistical analysis, and making distribution maps. The results showed that there was a significant relationship between the NGRDI pixel value and the nitrogen content, with a correlation value of -0.42. The regression equation results obtained from the NGRDI value with nitrogen content are y = -0.269x + 0.139. The accuracy test was carried out by the RMSE method, and the result was 0.027. This shows that the NGRDI index can estimate soil nitrogen content in Pamekasan Regency

Multi-Parametric Approach to Management Zone Delineation in a Hazelnut Grove in Italy

The increase in high-density hazelnut (Corylus avellana) areas drives the interest in practices of precision management. This work addressed soil apparent electrical conductivity (ECa), RGB aerial (UAV) images, proximal sensing, and field scouting in delineating and validating management zones (MZs) in a 2.96 ha hazelnut grove in Italy. ECa data were fitted to a semi-variogram, interpolated (simple kriging), and clustered, resulting in two MZs that were subjected to soil analysis. RGB imagery was used to extract tree canopies from the soil background and determine two vegetation indices (VIs) of general crop status: the Visible Atmospherically Resistant Index (VARI) and the Normalized Green-Red Difference Index (NGRDI). Then, plant growth parameters were manually assessed (tree height, crown size, etc.) and a proximal VI, the Canopy Index (CI), was determined with the MECS-VINE® vertical multisensor. MZ1 was characterized by lower ECa values than MZ2. This was associated with a lower clay content (9% vs. 21% in MZ1 vs. MZ2) and organic matter content (1.03% vs. 1.51% in MZ1 vs. MZ2), indicating lower soil fertility in MZ1 vs. MZ2. Additionally, hazelnut trees had significantly smaller canopies (1.42 vs. 1.94 m2 tree−1) and slightly lower values of VARI, NGRDI, and CI in MZ1 vs. MZ2. In conclusion, our approach used ECa to identify homogeneous field areas, which showed differences in soil properties influencing tree growth. This is the premise for differential hazelnut management in view of better efficiency and sustainability in the use of crop inputs.

Evaluating the saturation effect of vegetation indices in forests using 3D radiative transfer simulations and satellite observations

Vegetation indices (VIs) have been used extensively for qualitative and quantitative remote sensing monitoring of vegetation vigor and growth dynamics. However, the saturation phenomenon of VIs (i.e., insignificant change at moderate to high vegetation densities) poses a known limitation to their ability to characterize surface vegetation over the dense canopy. Although the mechanisms underlying saturation are relatively straightforward and several VIs have been proposed to mitigate the saturation effect, the assessment of the saturation effect of VIs remains insufficient. Notably, no unified metric has been proposed to quantify the VI saturation phenomenon, limiting VI selection in practical applications. In this study, we proposed two indicators to describe the saturation phenomenon and utilized a well-validated three-dimensional (3D) canopy radiative transfer (RT) model large-scale remote sensing data and image simulation framework (LESS) to simulate the bidirectional reflectance factor (BRF) of six forests scenes and assessed the variations in VIs in relation to leaf area index (LAI) values over different backgrounds, sun-sensor geometries, and spatial distribution types. The saturation characteristics of 36 VIs were evaluated in combination with simulation results and satellite observations from multiple sensors. The ranking of VI saturation from simulated and satellite results revealed a good agreement. Our results indicated that the simple ratio vegetation index (SR) performed best with the highest saturation point and can well characterize the surface vegetation condition until LAI reaches 4. Besides, we found that the saturation effect of VIs was influenced by soil brightness, sun-sensor geometry, and canopy structure. SR, modified simple ratio (MSR) and normalized green red difference index (NGRDI) were the most susceptible to these disturbing factors, although they had higher resistance to saturation. Modified triangular vegetation index 1 (MTVI1), modified non-linear vegetation index (MNLI), triangular greenness index (TGI), and triangular vegetation index (TriVI) performed well overall, combining the ability to resist saturation and disturbance factors. Appropriate application of VIs can help better understand vegetation responses to climate change and accurately assess ecosystem status. Our results contribute to the understanding of the VI saturation effect and provide a combined model and satellite data experimental workflow in appropriate VI selection to accurately characterize vegetation.

Vegetation Indices for Predicting the Growth and Harvest Rate of Lettuce

Urbanization has provided greater demand for food, and the search for strategies capable of reducing waste is essential to ensure food security. Lettuce (Lactuca sativa L.) culture has a short life cycle and its harvest point is determined visually, causing waste and important losses. Using vegetation indices could be an important alternative to reduce errors during harvest definition. The objective of this study was to evaluate different vegetation indices to predict the growth rate and harvest point of lettuce. Twenty-five genotypes of biofortified green lettuce were evaluated. The Green Leaf Index (GLI), Normalized Green Red Difference Index (NGRDI), Spectral Slope Saturation Index (SI), and Overall Hue Index (HUE) were calculated from images captured at 1, 8, 18, 24, and 36 days after transplanting (vegetative state). The diameter and average leaf area of plants were measured using QGIS software. Green mass, number of leaves, and plant and stem diameter were measured in the field. The means were compared using the Scott–Knott test (p ≤ 0.05) and simple linear regression models were generated to monitor the growth rate, obtaining R2 values ranging from 62% to 99%. Genetic dissimilarity was confirmed by the multivariate analysis presenting a cophenetic correlation coefficient of 88.49%. Furthermore, validation between data collected in the field versus data obtained by imaging was performed using Pearson’s correlations and showed moderate to high values. Overall, the vegetation indices SI, GLI, and NGRDI were efficient for monitoring the growth rate and determining the harvest point of different green lettuce genotypes, in attempts to reduce waste and losses. It is suggested that the definition of the harvest point based on vegetation indices are specific for each genotype.

A Novel Desert Vegetation Extraction and Shadow Separation Method Based on Visible Light Images from Unmanned Aerial Vehicles

Owing to factors such as climate change and human activities, ecological and environmental problems of land desertification have emerged in many regions around the world, among which the problem of land desertification in northwestern China is particularly serious. To grasp the trend of land desertification and the degree of natural vegetation degradation in northwest China is a basic prerequisite for managing the fragile ecological environment there. Visible light remote sensing images taken by a UAV can monitor the vegetation cover in desert areas on a large scale and with high time efficiency. However, as there are many low shrubs in desert areas, the shadows cast by them are darker, and the traditional RGB color-space-based vegetation index is affected by the shadow texture when extracting vegetation, so it is difficult to achieve high accuracy. For this reason, this paper proposes the Lab color-space-based vegetation index L2AVI (L-a-a vegetation index) to solve this problem. The EXG (excess green index), NGRDI (normalized green-red difference index), VDVI (visible band difference vegetation index), MGRVI (modified green-red vegetation index), and RGBVI (red-green-blue vegetation index) constructed based on RGB color space were used as control experiments in the three selected study areas. The results show that, although the extraction accuracies of the vegetation indices constructed based on RGB color space all reach more than 70%, these vegetation indices are all affected by the shadow texture to different degrees, and there are many problems of misdetection and omission. However, the accuracy of the L2AVI index can reach 99.20%, 99.73%, and 99.69%, respectively, avoiding the problem of omission due to vegetation shading and having a high extraction accuracy. Therefore, the L2AVI index can provide technical support and a decision basis for the protection and control of land desertification in northwest China.

Site-Specific Seed Yield Prediction of Red Fescue (Festuca rubra L.) Based on Drone Imaging and Local Regression Models

Yield maps give farmers information about growth conditions and can be a tool for site-specific crop management. Combine harvesters may provide farmers with detailed yield maps if there is a constant flow of a certain amount of biomass through the yield sensor. This is unachievable for grass seeds because the weight of the intake is generally too small to record the variation. Therefore, there is a need to find another way to make grass seed yield maps. We studied seed yield variation in two red fescue (Festuca rubra) fields with variation in management and soil fertility, respectively. We estimated five vegetation indices (VI) based on RGB images taken from a drone to describe yield variation, and trained prediction models based on relatively few harvested plots. Only results from the VI showing the strongest correlation between the index and the yield are presented (Normalized Excess Green Index (ExG) and Normalized Green/Red Difference Index (NGRDI)). The study indicates that it is possible to predict the yield variation in a grass field based on relatively few harvested plots, provided the plots represent contrasting yield levels. The prediction errors in yield (RMSE) ranged from 171 kg ha−1 to 231 kg ha−1, with no clear influence of the size of the training data set. Using random selection of plots instead of selecting plots representing contrasting yield levels resulted in slightly better predictions when evaluated on an average of ten random selections. However, using random selection of plots came with a risk of poor predictions due to the occasional lack of correlation between yield and VI. The exact timing of unmanned aerial vehicles (UAVs) image capture showed to be unimportant in the weeks before harvest.

Pedigree‐management‐flight interaction for temporal phenotype analysis and temporal phenomic prediction

AbstractUnoccupied aerial systems (UAS, aka drones) provide high dimensional temporal phenotype data for predictive plant breeding and genetic dissection. Methods to assess temporal phenotype data are an emerging need to predict temporal breeding values of genotypes. Here a novel interaction design was developed and evaluated to include drone flight dates as a component into the mixed model; allowing the temporal changes of drone image derived traits of maize hybrids across different flight dates as well as different management conditions to be monitored. Across 2017 and 2019 respectively, 228 and 100 maize hybrids were grown under two types of management (optimal and late plantings). Seven drone surveys were conducted over each management in 2017 while five drone surveys were conducted over each management in 2019. Temporal plant height (canopy height measurements, CHM) and normalized green‐red difference index (NGRDI) were extracted from each drone survey and used as phenotype data to evaluate the interaction design. Day of flight effects explained the highest amount of total variation for grain yield in the interaction model, meaning the majority of phenotypic variation of CHM and NGRDI occurred across growth with a unique temporal trajectory in each management system. Temporal repeatability values remained higher than 0.5 for CHM and NGRDI in each year. Temporal CHM and NGRDI breeding values of maize hybrids were combined in ridge and lasso regression prediction models. Yield prediction ability of untested genotypes in untested environments were predicted higher by using pedigree × management × flight (PMF) and pedigree× management (PM) interaction results (∼0.34 and 0.52 in 2017 and 2019). Combining environment specific phenomic data (PMF plus PM) gave a larger improvement in yield prediction when the tested and untested environments were less similar. Overall, combined temporal phenomic data could moderately predict grain yield under the most challenging predictive breeding scenario, untested and unrelated genotypes in untested environments.

Monitoring Dewatering Fish Spawning Sites in the Reservoir of a Large Hydropower Plant in a Lowland Country Using Unmanned Aerial Vehicles

This paper presents research concerning dewatered areas in the littoral zones of the Kaunas hydropower plant (HPP) reservoir in Lithuania. It is a multipurpose reservoir that is primarily used by two large hydropower plants for power generation. As a result of the peaking operation regime of the Kaunas HPP, the large quantity of water that is subtracted and released into the reservoir by the Kruonis pumped storage hydropower plant (PSP), and the reservoir morphology, i.e., the shallow, gently sloping littoral zone, significant dewatered areas can appear during drawdown operations. This is especially dangerous during the fish spawning period. Therefore, reservoir operation rules are in force that limit the operation of HPPs and secure other reservoir stakeholder needs. There is a lack of knowledge concerning fish spawning locations, how they change, and what areas are dewatered at different stages of HPP operation. This knowledge is crucial for decision-making and efficient reservoir storage management in order to simultaneously increase power generation and protect the environment. Current assessments of the spawning sites are mostly based on studies that were carried out in the 1990s. Surveying fish spawning sites is typically a difficult task that is usually carried out by performing manual bathymetric measurements due to the limitations of sonar in such conditions. A detailed survey of a small (approximately 5 ha) area containing several potential spawning sites was carried out using Unmanned Aerial Vehicles (UAV) equipped with multispectral and conventional RGB cameras. The captured images were processed using photogrammetry and analyzed using various techniques, including machine learning. In order to highlight water and track changes, various indices were calculated and assessed, such as the Normalized Difference Water Index (NDWI), Normalized Difference Vegetation Index (NDVI), Visible Atmospherically Resistant Index (VARI), and Normalized Green-Red Difference Index (NGRDI). High-resolution multispectral images were used to analyze the spectral footprint of aquatic macrophytes, and the possibility of using the results of this study to identify and map potential spawning sites over the entire reservoir (approximately 63.5 km2) was evaluated. The aim of the study was to investigate and implement modern surveying techniques to improve usage of reservoir storage during hydropower plant drawdown operations. The experimental results show that thresholding of the NGRDI and supervised classification of the NDWI were the best-performing methods for the shoreline detection in the fish spawning sites.

Quantitatively identify the factors driving loess erodibility variations after ecological restoration

AbstractThe soil erodibility factor is the key index to evaluate regional potential erosion degree and predict soil erosion. Therefore, it is very meaningful to explore the response mechanism of soil erodibility to external environmental factors on the Loess Plateau after ecological restoration. In this study, three soil erodibility factors (K‐USLE, K‐shira, and K‐torri), a comprehensive soil erodibility index (CSEI), different vegetation indices, and five topographic parameters (Topo) in two typical watersheds were selected to achieve this goal. The results revealed that vegetation indices (VI), soil properties (SP), and Topo were significantly affected by regional location and land use types. Vegetation restoration can reduce soil erodibility factors, but the reduction capacity of different land use types varies greatly. SP, VI, and Topo can explain more than 70% of variances in soil erodibility. The partial least squares‐structural equation modelling (PLS‐SEM) illustrated that SP had the largest and significant direct impact on soil erodibility (standard path coefficients (SPC) were −0.935 and −0.895, respectively). Vegetation indirectly inhibited the soil erodibility of the two watersheds by improving SP (SPC were 0.228 and 0.437, respectively), while the indirect impact of Topo mainly interferes with soil erodibility by adjusting vegetation type and spatial distribution (SPC were −0.566 and −0.292, respectively). Furthermore, CSEI was significantly affected by land use, and significantly associated with soil texture, soil organic carbon (SOC), mean weight diameter (MWD), normalized green‐red difference index (NGRDI), and vegetative index (VEG) in both watersheds. The soil in the Xinshui River watershed was more vulnerable to erosion than that in the Zhujiachuan watershed. It is of great significance to explore the response mechanism of soil erodibility to multi‐scale factors to understand the impact of external environmental evolution on soil erosion and sediment yield on the Loess Plateau.

High-Throughput Plant Phenotyping (HTPP) in Resource-Constrained Research Programs: A Working Example in Ghana

In this paper, we present a procedure for implementing field-based high-throughput plant phenotyping (HTPP) that can be used in resource-constrained research programs. The procedure relies on opensource tools with the only expensive item being one-off purchase of a drone. It includes acquiring images of the field of interest, stitching the images to get the entire field in one image, calculating and extracting the vegetation indices of the individual plots, and analyzing the extracted indices according to the experimental design. Two populations of groundnut genotypes with different maturities were evaluated for their reaction to early and late leaf spot (ELS, LLS) diseases under field conditions in 2020 and 2021. Each population was made up of 12 genotypes in 2020 and 18 genotypes in 2021. Evaluation of the genotypes was done in four locations in each year. We observed a strong correlation between the vegetation indices and the area under the disease progress curve (AUDPC) for ELS and LLS. However, the strength and direction of the correlation depended upon the time of disease onset, level of tolerance among the genotypes and the physiological traits the vegetation indices were associated with. In 2020, when the disease was observed to have set in late in medium duration population, at the beginning of the seed stage (R5), normalized green-red difference index (NGRDI) and variable atmospheric resistance index (VARI) derived at the beginning pod stage (R3) had a positive relationship with the AUDPC for ELS, and LLS. On the other hand, NGRDI and VARI derived from images taken at R5, and physiological maturity (R7) had negative relationships with AUDPC for ELS, and LLS. In 2021, when the disease was observed to have set in early (at R3) also in medium duration population, a negative relationship was observed between NGRDI and VARI and AUDPC for ELS and LLS, respectively. We found consistently negative relationships of NGRDI and VARI with AUDPC for ELS and LLS, respectively, within the short duration population in both years. Canopy cover (CaC), green area (GA), and greener area (GGA) only showed negative relationships with AUDPC for ELS and LLS when the disease caused yellowing and defoliation. The rankings of some genotypes changed for NGRDI, VARI, CaC, GA, GGA, and crop senescence index (CSI) when lesions caused by the infections of ELS and LLS became severe, although that did not affect groupings of genotypes when analyzed with principal component analysis. Notwithstanding, genotypes that consistently performed well across various reproductive stages with respect to the vegetation indices constituted the top performers when ELS, LLS, haulm, and pod yields were jointly considered.

Individual Tree Detection in Coal Mine Afforestation Area Based on Improved Faster RCNN in UAV RGB Images

Forests are the most important part of terrestrial ecosystems. In the context of China’s industrialization and urbanization, mining activities have caused huge damage to the forest ecology. In the Ulan Mulun River Basin (Ordos, China), afforestation is standard method for reclamation of coal mine degraded land. In order to understand, manage and utilize forests, it is necessary to collect local mining area’s tree information. This paper proposed an improved Faster R-CNN model to identify individual trees. There were three major improved parts in this model. First, the model applied supervised multi-policy data augmentation (DA) to address the unmanned aerial vehicle (UAV) sample label size imbalance phenomenon. Second, we proposed Dense Enhance Feature Pyramid Network (DE-FPN) to improve the detection accuracy of small sample. Third, we modified the state-of-the-art Alpha Intersection over Union (Alpha-IoU) loss function. In the regression stage, this part effectively improved the bounding box accuracy. Compared with the original model, the improved model had the faster effect and higher accuracy. The result shows that the data augmentation strategy increased AP by 1.26%, DE-FPN increased AP by 2.82%, and the improved Alpha-IoU increased AP by 2.60%. Compared with popular target detection algorithms, our improved Faster R-CNN algorithm had the highest accuracy for tree detection in mining areas. AP was 89.89%. It also had a good generalization, and it can accurately identify trees in a complex background. Our algorithm detected correct trees accounted for 91.61%. In the surrounding area of coal mines, the higher the stand density is, the smaller the remote sensing index value is. Remote sensing indices included Green Leaf Index (GLI), Red Green Blue Vegetation Index (RGBVI), Visible Atmospheric Resistance Index (VARI), and Normalized Green Red Difference Index (NGRDI). In the drone zone, the western area of Bulianta Coal Mine (Area A) had the highest stand density, which was 203.95 trees ha−1. GLI mean value was 0.09, RGBVI mean value was 0.17, VARI mean value was 0.04, and NGRDI mean value was 0.04. The southern area of Bulianta Coal Mine (Area D) was 105.09 trees ha−1 of stand density. Four remote sensing indices were all the highest. GLI mean value was 0.15, RGBVI mean value was 0.43, VARI mean value was 0.12, and NGRDI mean value was 0.09. This study provided a sustainable development theoretical guidance for the Ulan Mulun River Basin. It is crucial information for local ecological environment and economic development.

Mapping of coastal surface chlorophyll-a concentration by multispectral reflectance measurement from unmanned aerial vehicles

In subtropical coastal waters, the explosive growth of phytoplankton under favorable conditions can lead to water discolouration and massive fish kills. Manual field sampling and laboratory analysis of chlorophyll-a concentration (Chl-a) as an indicator to algal biomass, is resources intensive and time consuming, delaying responses to disastrous harmful algal blooms. Cloudy weather often precludes the use of satellite images for water quality and algal bloom monitoring. This study aims at developing an estimator algorithm for quantitative mapping of surface Chl-a for coastal waters, based on surface reflectance measurement from an Unmanned Aerial Vehicle (UAV) with a five-band multispectral camera. The surface reflectance is obtained from calibrated multispectral images which are radiometric-corrected against incoming solar radiation. It is found that Chl-a has an inverse correlation with the Normalized Green-Red Difference Index (NGRDI). A regression estimator model for Chl-a from NGRDI is developed, showing excellent performance for fish farms in coastal waters with different characteristics. The technology is demonstrated for mapping the spatial and temporal variation of Chl-a during an algal bloom, offering a useful complement to traditional field monitoring for fisheries management and emergency response.

A New Method for Estimating Soil Fertility Using Extreme Gradient Boosting and a Backpropagation Neural Network

Soil fertility affects crop yield and quality. A quick, accurate evaluation of soil fertility is crucial for agricultural production. Few satellite image-based evaluation studies have quantified soil fertility during the crop growth period. Therefore, this study proposes a new approach to the quantitative evaluation of soil fertility. Firstly, the optimal crop spectral variables were selected using the integration of an extreme gradient boosting (XGBoost) algorithm with variance inflation factor (VIF). Then, based on the optimal crop spectral variables where the red-edge indices were introduced for the first time, the estimation models were developed using the backpropagation neural network (BPNN) algorithm to assess soil fertility. The model was finally adopted to map the soil fertility using Sentinel-2 imagery. This study was performed in the Conghua District of Guangzhou, Guangdong Province, China. The results of our research are as follows: (1) five crop spectral variables (inverted red-edge chlorophyll index (IRECI), chlorophyll vegetation index (CVI), normalized green-red difference index (NGRDI), red-edge position (REP), and triangular greenness index (TGI)) were the optimal variables. (2) The BPNN model established with optimal variables provided reliable estimates of soil fertility, with the determination coefficient (R2) of 0.66 and a root mean square error (RMSE) of 0.17. A nonlinear relation was found between soil fertility and the optimal crop spectral variables. (3) The BPNN model provides the potential for soil fertility mapping using Sentinel-2 images, with an R2 of 0.62 and an RMSE of 0.09 for the measured and estimated results. This study suggests that the proposed method is suitable for the estimation of soil fertility in paddy fields.

Assessing Phytosanitary Application Efficiency of a Boom Sprayer Machine Using RGB Sensor in Grassy Fields

The systematic use of plant protection products is now being called into question with the growing awareness of the risks they can represent for the environment and human health. The application of precision agriculture technologies helps to improve agricultural production but also to rationalize input costs and improve ecological footprints. Here we present a study on fungicide application efficiency and its impact on the grass quality of a golf course green using the free open-source image analysis software FIJI (Image J) to analyze ground RGB (high-resolution digital cameras) and multispectral aerial imagery in combination with experimental data of spray pressure and hydraulic slot nozzle size of a boom sprayer machine. The multivariate regression model best explained variance in the normalized green-red difference index (NGRDI) as a relevant indicator of healthy turfgrass fields from the aerial, ground, and machine data set.

Improving Sustainable Vegetation Indices Processing on Low-Cost Architectures

The development of embedded systems in sustainable precision agriculture has provided an important benefit in terms of processing time and accuracy of results, which has influenced the revolution in this field of research. This paper presents a study on vegetation monitoring algorithms based on Normalized Green-Red Difference Index (NGRDI) and Visible Atmospherically Resistant Index (VARI) in agricultural areas using embedded systems. These algorithms include processing and pre-processing to increase the accuracy of sustainability monitoring. The proposed algorithm was evaluated on a real database in the Souss Massa region in Morocco. The collection of data was based on unmanned aerial vehicles images hand data using four different agricultural products. The results in terms of processing time have been implemented on several architectures: Desktop, Odroid XU4, Jetson Nano, and Raspberry. However, this paper introduces a thorough study of the Hardware/Software Co-Design approach to choose the most suitable system for our proposed algorithm that responds to the different temporal and architectural constraints. The evaluation proved that we could process 311 frames/s in the case of low resolution, which gives real-time processing for agricultural field monitoring applications. The evaluation of the proposed algorithm on several architectures has shown that the low-cost XU4 card gives the best results in terms of processing time, power consumption, and computation flexibility.