Normalized Difference Red Edge Index Research Articles

Precision Estimation of Crop Coefficient for Maize Cultivation Using High-Resolution Satellite Imagery to Enhance Evapotranspiration Assessment in Agriculture.

The estimation of crop evapotranspiration (ETc) is crucial for irrigation water management, especially in arid regions. This can be particularly relevant in the Po Valley (Italy), where arable lands suffer from drought damages on an annual basis, causing drastic crop yield losses. This study presents a novel approach for vegetation-based estimation of crop evapotranspiration (ETc) for maize. Three years of high-resolution multispectral satellite (Sentinel-2)-based Normalized Difference Vegetation Index (NDVI), Normalized Difference Water Index (NDWI), Normalized Difference Red Edge Index (NDRE), and Leaf Area Index (LAI) time series data were used to derive crop coefficients of maize in nine plots at the Acqua Campus experimental farm of Irrigation Consortium for the Emilia Romagna Canal (CER), Italy. Since certain vegetation indices (VIs) (such as NDVI) have an exponential nature compared to the other indices, both linear and power regression models were evaluated to estimate the crop coefficient (Kc). In the context of linear regression, the correlations between Food and Agriculture Organization (FAO)-based Kc and NDWI, NDRE, NDVI, and LAI-based Kc were 0.833, 0.870, 0.886, and 0.771, respectively. Strong correlation values in the case of power regression (NDWI: 0.876, NDRE: 0.872, NDVI: 0.888, LAI: 0.746) indicated an alternative approach to provide crop coefficients for the vegetation period. The VI-based ETc values were calculated using reference evapotranspiration (ET0) and VI-based Kc. The weather station data of CER were used to calculate ET0 based on Penman-Monteith estimation. Out of the Vis, NDWI and NDVI-based ETc performed the best both in the cases of linear (NDWI RMSE: 0.43 ± 0.12; NDVI RMSE: 0.43 ± 0.095) and power (NDWI RMSE: 0.44 ± 0.116; NDVI RMSE: 0.44 ± 0.103) approaches. The findings affirm the efficacy of the developed methodology in accurately assessing the evapotranspiration rate. Consequently, it offers a more refined temporal estimation of water requirements for maize cultivation in the region.

Detection of pine wood nematode infestation using hyperspectral drone images

The pine wood nematode (PWN) is one of the most threatening pests of pine forests in China. An essential management response involves felling and removing the infested trees before the PWN can spread over large areas, but this requires early detection. In this study, we analyzed the temporal changes in the spectral signatures of healthy and infested trees throughout the entire growing season (May to October) using unmanned aerial vehicle (UAV) hyperspectral images (HSI). To explore the potential of these seasonal spectral changes in detecting PWN, we investigated the separability of healthy and infested trees using both absolute values and changes in values. A random forest (RF) classifier was applied to separate healthy and infested trees based on reflectances, derivatives, vegetation indices (VIs), and their seasonal change values. We obtained the following results: (1) the seasonal changes of tree crown spectra following a PWN infestation were similar to those observed using absolute spectral values, though the latter performed better in early detection; (2) in the early stage of infestation (June and July), the green and red-edge regions contained the most sensitive bands, whereas the near-infrared portion played a more important role in later stages; (3) derivatives achieved higher overall accuracy (OA) than the reflectance in the early stage of PWN infestation (June and July); (4) in June, the Pigment Specific Simple Ratio (PSSR) had the highest separability (OA = 0.77) for PWN infestation, followed by the Plant Senescence Reflectance Index (PSRI) and the Normalized Difference Red-edge Index (NDRE), both with a OA of 0.70. In July, PSRI, Red Edge Normalized Difference Vegetation Index (RENDVI), and the Modified Red Edge Simple Ratio (MRESR) had the highest OA of 0.77. These VIs were calculated based on the red-edge regions. In August, RENDVI achieved the best accuracy (0.87), followed by the Normalized Difference Vegetation Index (NDVI, OA = 0.83) and the Plant Stress Index (PSI, OA = 0.80). In September and October, PSRI, PSI, the Greenness Index (GI), and RENDVI showed the strongest separability (0.87 to 0.90). Similar results were obtained using change values of VIs. In June, RENDVI, PSI, and NDVI showed the highest accuracy (OA = 0.73). In July, MRESR had the best accuracy (0.77), followed by RENDVI (0.73) and PSI (0.73). In the late stage of PWN infestation, NDVI, PSRI, PSI, RENDVI, GI, and PSSR obtained the highest accuracies (0.83 to 0.93), surpassing the accuracy achieved using absolute spectra; (5) in RF classification model, seasonal variation values derived from reflectances, derivatives, and VIs performed well in the late stages of the PWN infestation (September and October) but were not as good as absolute values in the early and middle stages (June to August). Our study explored the potential of seasonal changes in UAV spectra for early detection of forest pests.

Integrating plant morphological traits with remote-sensed multispectral imageries for accurate corn grain yield prediction.

Sustainable crop production requires adequate and efficient management practices to reduce the negative environmental impacts of excessive nitrogen (N) fertilization. Remote sensing has gained traction as a low-cost and time-efficient tool for monitoring and managing cropping systems. In this study, vegetation indices (VIs) obtained from an unmanned aerial vehicle (UAV) were used to detect corn (Zea mays L.) response to varying N rates (ranging from 0 to 208 kg N ha-1) and fertilizer application methods (liquid urea ammonium nitrate (UAN), urea side-dressing and slow-release fertilizer). Four VIs were evaluated at three different growth stages of corn (V6, R3, and physiological maturity) along with morphological traits including plant height and leaf chlorophyll content (SPAD) to determine their predictive capability for corn yield. Our results show no differences in grain yield (average 13.2 Mg ha-1) between furrow-applied slow-release fertilizer at ≥156 kg N ha-1 and 208 kg N ha-1 side-dressed urea. Early season remote-sensed VIs and morphological data collected at V6 were least effective for grain yield prediction. Moreover, multivariate grain yield prediction was more accurate than univariate. Late-season measurements at the R3 and mature growth stages using a combination of normalized difference vegetation index (NDVI) and green normalized difference vegetation index (GNDVI) in a multilinear regression model showed effective prediction for corn yield. Additionally, a combination of NDVI and normalized difference red edge index (NDRE) in a multi-exponential regression model also demonstrated good prediction capabilities.

A chlorophyll-constrained semi-empirical model for estimating leaf area index using a red-edge vegetation index

The parameter of leaf area index (LAI) provides valuable information about plant growth. Most remote sensing methods for estimating LAI are based on the vegetation indices (VIs) in the visible and near-infrared (VNIR) spectral regions. Recently, these VIs calculated with one or more bands in the red-edge region become popular in estimating LAI. Since the reflectance in the red-edge spectral region is sensitive to both LAI and leaf chlorophyll content (LCC), most red-edge-based VIs (such as normalized difference red edge index, NDRE) should be used to estimate the canopy chlorophyll content (the product of LAI and LCC) in theory. When applying NDRE to estimating LAI alone, their relationship is affected by the variation in LCC. To mitigate the LCC effect on LAI estimation using NDRE, this study proposed a chlorophyll-constrained semi-empirical model (CSE). The coefficients of the CSE model were determined from the simulated dataset based on radiative transfer models, and hence were not dependent on measured data. The performance of the CSE model on LAI estimation was evaluated using simulated and measured datasets, including a crop dataset collected in China and a multi-ecosystem dataset obtained from the National Ecological Observatory Network (NEON). The results showed that the root mean square error (RMSE) obtained by the CSE model ranged from 0.58 to 0.72 m2/m2 for the simulated and measured datasets. In particular, the CSE model also exhibited wide generality across multiple vegetation types for the NEON dataset, with the RMSE in the range of 0.33–0.86 m2/m2. In comparison, without considering the LCC effect, the model would lead to clear underestimation or overestimation of LAI (RMSE = 1.26–2.11 m2/m2). These findings improved our understanding of the LCC effects on universal LAI predictive models and would facilitate the accurate mapping of LAI over large regions for monitoring plant growth status.

Crop Type Classification using Sentinel 2A-Derived Normalized Difference Red Edge Index (NDRE) and Machine Learning Approach

Abstract Satellite remote sensing (RS) enables the extraction of vital information on land cover and crop type. Land cover and crop type classification using RS data and machine learning (ML) techniques have garnered considerable attention in the scientific community. This study aimed to enhance the field of remote sensing research using high-resolution satellite imagery and the ML approach. To reach this goal, machine learning techniques were used to demonstrate whether they could accurately classify different types of crops in farming areas using the Normalized Difference Red Edge Index (NDRE) from Sentinel 2A. Five ML classifiers, namely Support Vector Machines (SVM), Random Forest (RF), Decision Tree (DT), K-Nearest Neighbors (KNN), and Multi-Layer Perceptron (MLP) were implemented using Python programming on Google Colaboratory. The target crop classes included cereals, fallow, forage, fruits, grassland-pasture, legumes, maize, sugar beet, onion-garlic, sunflower, and watermelon-melon. The classification models exhibited strong performance, as evidenced by their robust overall accuracy (OA). The RF model outperformed, with an OA rate of 95% and a Kappa score of 92%. It was followed by DT, KNN, SVM, and MLP with an OA of 88%, 87%, 85%, and 82%, respectively. This study confirmed the potential enhancement of the application of high spatial resolution satellite RS data and ML for crop type classification, especially in regions that have received less attention in previous studies.

Assessing relationships of cover crop biomass and nitrogen content to multispectral imagery

AbstractCover crops provide valuable roles in sustainable agriculture, provided they produce enough biomass. To accurately measure their services to field management, spatial estimates would be useful to producers. This study used multispectral drone imagery to produce maps of normalized difference vegetation index (NDVI), normalized difference red edge index (NDRE), and a digital surface model (DSM) of cover crop plots on sandy, Mid‐Atlantic soils. Cover crops included cereal rye (Secale cereale), mixtures of rye and crimson clover (Trifolium incarnatum), and mixtures of rye and hairy vetch (Vicia villosa). Their biomass was sampled in the spring of 2019, 2020, and 2021, dried, weighed, and analyzed for total nitrogen (N) content. Measurements of NDVI became saturated (i.e., reached a linear plateau) at 3.86 Mg biomass ha−1, NDRE at 5.72 Mg biomass ha−1, and the DSM at 5.11 Mg biomass ha−1. The measured N content became saturated at 80.9, 139.1, and 75 kg N ha−1 for NDVI, NDRE, and the DSM, respectively. Based on log transformations, NDVI was a stronger predictor of biomass and N, but not C:N. The NDRE was important for biomass, N, and C:N, while the DSM interactions with cover crop species helped predict both the N content and C:N of cover crop tissues. Accumulated growing degree days was important as an individual variable for biomass and N and as an interaction with cover crop species.

Assessing Forest Species Diversity in Ghana’s Tropical Forest Using PlanetScope Data

This study utilized a remotely sensed dataset with a high spatial resolution of 3 m to predict species diversity in the Bobiri Forest Reserve (BFR), a moist semi-deciduous tropical forest in Ghana. We conducted a field campaign of tree species measurements to achieve this objective for species diversity estimation. Thirty-five field plots of 50 m × 20 m were established, and the most dominant tree species within the forest were identified. Other measurements, such as diameter at breast height (DBH ≥ 5 cm), tree height, and each plot’s GPS coordinates, were recorded. The following species diversity indices were estimated from the field measurements: Shannon–Wiener (H′), Simpson diversity index (D2), species richness (S), and species evenness (J′). The PlanetScope surface reflectance data at 3 m spatial resolution was acquired and preprocessed for species diversity prediction. The spectral/pixel information of all bands, except the coastal band, was extracted for further processing. Vegetation indices (VIs) (NDVI—normalized difference vegetation index, EVI—enhanced vegetation index, SRI—simple ratio index, SAVI—soil adjusted vegetation index, and NDRE—normalized difference red edge index) were also calculated from the spectral bands and their pixel value extracted. A correlation analysis was then performed between the spectral bands and VIs with the species diversity index. The results showed that spectral bands 6 (red) and 2 (blue) significantly correlated with the two main species diversity indices (S and H′) due to their influence on vegetation properties, such as canopy biomass and leaf chlorophyll content. Furthermore, we conducted a stepwise regression analysis to investigate the most important spectral bands to consider when estimating species diversity from the PlanetScope satellite data. Like the correlation results, bands 6 (red) and 2 (blue) were the most important bands to be considered for predicting species diversity. The model equations from the stepwise regression were used to predict tree species diversity. Overall, the study’s findings emphasize the relevance of remotely sensed data in assessing the ecological condition of protected areas, a tool for decision-making in biodiversity conservation.

Delineation of Soil Management Zones and Validation through the Vigour of a Fodder Crop

In Mediterranean farming systems, the semi-arid conditions and agricultural ecosystems have made site-specific management an important approach. This method aims to understand and handle the variability of soil properties and crop management, particularly through the utilization of geospatial information and accessible technology. Over three years in a 30 ha experimental field located in the Alentejo region (Portugal), crop establishment was monitored using data from soil apparent electrical conductivity (ECa), remote sensing (Sentinel-2), and in situ soil sampling. The procedure began with Step 1, involving the acquisition of soil spatial information and spatial interpolation. Subsequently, in Step 2, management zones (MZs) for soil characteristics were delineated using a combination of ECa measurements and soil analysis, and Step 3 spanned over three years of gathering meteorological data and crop remote sensing measurements. In Step 4, site-specific crop MZs were delineated by vegetation indexes (VIs). This article aims to increase the importance of in situ and remote assessments to more accurately identify areas with different productive potential. Results showed three MZs based on the percentage of sand, ECa, altimetry, exchangeable calcium, and exchangeable calcium properties, validated by crop VIs (Normalized Difference Vegetation Index (NDVI), Normalized Difference Red-Edge Index (NDRE), and Normalized Difference Moisture Index (NDMI)) over time. Although there are many sensorial techniques available for site-specific management, this paper emphasizes a methodology for the farmer to identify different MZs combining remote and in situ evaluations, supporting new opportunities for a more rational use of natural resources. Based on soil parameters, three site-specific management areas could be selected. NDMI was the index that best explained the MZs created according to soil properties.

Using UAV-Based Temporal Spectral Indices to Dissect Changes in the Stay-Green Trait in Wheat.

Stay-green (SG) in wheat is a beneficial trait that increases yield and stress tolerance. However, conventional phenotyping techniques limited the understanding of its genetic basis. Spectral indices (SIs) as non-destructive tools to evaluate crop temporal senescence provide an alternative strategy. Here, we applied SIs to monitor the senescence dynamics of 565 diverse wheat accessions from anthesis to maturation stages over 2 field seasons. Four SIs (normalized difference vegetation index, green normalized difference vegetation index, normalized difference red edge index, and optimized soil-adjusted vegetation index) were normalized to develop relative stay-green scores (RSGS) as the SG indicators. An RSGS-based genome-wide association study identified 47 high-confidence quantitative trait loci (QTL) harboring 3,079 single-nucleotide polymorphisms associated with SG and 1,085 corresponding candidate genes. Among them, 15 QTL overlapped or were adjacent to known SG-related QTL/genes, while the remaining QTL were novel. Notably, a set of favorable haplotypes of SG-related candidate genes such as TraesCS2A03G1081100, TracesCS6B03G0356400, and TracesCS2B03G1299500 are increasing following the Green Revolution, further validating the feasibility of the pipeline. This study provided a valuable reference for further quantitative SG and genetic research in diverse wheat panels.

Time Series from Sentinel-2 for Organic Durum Wheat Yield Prediction Using Functional Data Analysis and Deep Learning

Yield prediction is a crucial activity in scheduling agronomic operations and in informing the management and financial decisions of a wide range of stakeholders of the organic durum wheat supply chain. This research aims to develop a yield forecasting system by combining vegetation index time-series data from Sentinel-2 L2A time-series data, field-measured yields, and deep learning techniques. Remotely sensed data over a season could be, in general, noisy and characterized by a variable density due to weather conditions. This problem was mitigated using Functional Principal Component Analysis (FPCA). We obtained a functional representation of acquired data, and starting from this, we tried to apply deep learning to predict the crop yield. We used a Convolutional Neural Network (CNN) approach, starting from images that embed temporal and spectral dimensions. This representation does not require one to a priori select a vegetation index that, typically, is task-dependent. The results have been also compared with classical approaches as Partial Least Squares (PLS) on the main reference vegetation indexes such as the Normalized Difference Vegetation Index (NDVI) and Normalized Difference Red Edge index (NDRE), considering both in-season and end-season scenarios. The obtained results show that the image-based representation of multi-spectral time series could be an effective method to estimate the yield, also, in the middle stage of cropping with R2 values greater than 0.83. The developed model could be used to estimate yield the neighbor fields characterized by similar setups in terms of the crop, variety, soil, and, of course, management.

Combining multiple UAV-Based indicators for wheat yield estimation, a case study from Germany

ABSTRACT Unmanned aircraft vehicles (UAV) are widely used for yield estimations in agricultural production. Many significant improvements have been made towards the usage of hyperspectral and thermal sensors. The practical application of these new techniques meanwhile has been limited by the cost of data collection and the complexities of data processing. The objective of this paper is to evaluate the effectiveness of wheat yield estimations based on integrating vegetation indices (VI), solar radiation and crop height (CH), all of which are characterized by lower cost of data collection and processing. The VIs, solar radiation and CH were calculated based on UAV-based multispectral images obtained from two separate plots in Southern Germany and validated with data from a third plot. We compare the individual and joint predictive performance of different VIs, CH, and solar radiation by contrasting the estimated yield with actual yield based on multiple linear regression and quantile regression. The best predictive power was found for a combined estimation with CH, solar radiation and a Normalized Difference Red-edge Index (R2 = 0.75, RMSE = 0.53). This combined estimation resulted in a 15–20% improvement in the prediction of wheat yield accuracy as compared with utilizing any of the indices separately.

Pre-Harvest Corn Grain Moisture Estimation Using Aerial Multispectral Imagery and Machine Learning Techniques

Corn grain moisture (CGM) is critical to estimate grain maturity status and schedule harvest. Traditional methods for determining CGM range from manual scouting, destructive laboratory analyses, and weather-based dry down estimates. Such methods are either time consuming, expensive, spatially inaccurate, or subjective, therefore they are prone to errors or limitations. Realizing that precision harvest management could be critical for extracting the maximum crop value, this study evaluates the estimation of CGM at a pre-harvest stage using high-resolution (1.3 cm/pixel) multispectral imagery and machine learning techniques. Aerial imagery data were collected in the 2022 cropping season over 116 experimental corn planted plots. A total of 24 vegetation indices (VIs) were derived from imagery data along with reflectance (REF) information in the blue, green, red, red-edge, and near-infrared imaging spectrum that was initially evaluated for inter-correlations as well as subject to principal component analysis (PCA). VIs including the Green Normalized Difference Index (GNDVI), Green Chlorophyll Index (GCI), Infrared Percentage Vegetation Index (IPVI), Simple Ratio Index (SR), Normalized Difference Red-Edge Index (NDRE), and Visible Atmospherically Resistant Index (VARI) had the highest correlations with CGM (r: 0.68–0.80). Next, two state-of-the-art statistical and four machine learning (ML) models (Stepwise Linear Regression (SLR), Partial Least Squares Regression (PLSR), Artificial Neural Network (ANN), Support Vector Machine (SVM), Random Forest (RF), and K-nearest neighbor (KNN)), and their 120 derivates (six ML models × two input groups (REFs and REFs+VIs) × 10 train–test data split ratios (starting 50:50)) were formulated and evaluated for CGM estimation. The CGM estimation accuracy was impacted by the ML model and train-test data split ratio. However, the impact was not significant for the input groups. For validation over the train and entire dataset, RF performed the best at a 95:5 split ratio, and REFs+VIs as the input variables (rtrain: 0.97, rRMSEtrain: 1.17%, rentire: 0.95, rRMSEentire: 1.37%). However, when validated for the test dataset, an increase in the train–test split ratio decreased the performances of the other ML models where SVM performed the best at a 50:50 split ratio (r = 0.70, rRMSE = 2.58%) and with REFs+VIs as the input variables. The 95:5 train–test ratio showed the best performance across all the models, which may be a suitable ratio for relatively smaller or medium-sized datasets. RF was identified to be the most stable and consistent ML model (r: 0.95, rRMSE: 1.37%). Findings in the study indicate that the integration of aerial remote sensing and ML-based data-run techniques could be useful for reliably predicting CGM at the pre-harvest stage, and developing precision corn harvest scheduling and management strategies for the growers.

Monitoring of three stages of paddy growth using multispectral vegetation index derived from UAV images

Paddy cultivation in Malaysia plays a crucial role in food production, with a focus on improving crop quality and quantity. With current national self-sufficiency levels ranging between 67 and 70%, the Malaysian government intends to produce higher-quality crops and boost agricultural production. However, the prominent paddy-producing state of Kedah has witnessed a decline in yields over the years. To address this, the study explores the effectiveness of unmanned aerial vehicles (UAVs) equipped with vegetation indices (VIs) for monitoring paddy plant health at various growth stages. Researchers acquired aerial imagery during two seasons in 2019, capturing three distinct growth stages: tillering (40 days after sowing), flowering (60 days after sowing), and ripening (100 days after sowing). These stages represent critical points in the paddy plant's life cycle. Agisoft Metashape software processed the images to extract VIs data. The study found that the Normalized Difference Vegetation Index (NDVI) and Blue Normalized Difference Vegetation Index (BNDVI) exhibited over 90% similarity. In contrast, the Normalized Difference Red Edge Index (NDRE), utilizing near-infrared and red-edge light reflections, demonstrated a unique relationship. NDRE outperformed NDVI and BNDVI with an R-squared value of 0.842, showcasing its superior accuracy, especially for dense crops like paddy plants sensitive to subtle changes in vegetation. In conclusion, this research highlights the potential of UAV-based VIs for effectively monitoring paddy plant health during different growth stages. The NDRE index, in particular, proves valuable for assessing dense crops, offering insights for precision agriculture and crop management in Malaysia.

Identification of the Initial Anthesis of Soybean Varieties Based on UAV Multispectral Time-Series Images

Accurate and high-throughput identification of the initial anthesis of soybean varieties is important for the breeding and screening of high-quality soybean cultivars in field trials. The objectives of this study were to identify the initial day of anthesis (IADAS) of soybean varieties based on remote sensing multispectral time-series images acquired by unmanned aerial vehicles (UAVs), and analyze the differences in the initial anthesis of the same soybean varieties between two different climatic regions, Shijiazhuang (SJZ) and Xuzhou (XZ). First, the temporal dynamics of several key crop growth indicators and spectral indices were analyzed to find an effective indicator that favors the identification of IADAS, including leaf area index (LAI), above-ground biomass (AGB), canopy height (CH), normalized-difference vegetation index (NDVI), red edge chlorophyll index (CIred edge), green normalized-difference vegetation index (GNDVI), enhanced vegetation index (EVI), two-band enhanced vegetation index (EVI2) and normalized-difference red-edge index (NDRE). Next, this study compared several functions, like the symmetric gauss function (SGF), asymmetric gauss function (AGF), double logistic function (DLF), and fourier function (FF), for time-series curve fitting, and then estimated the IADAS of soybean varieties with the first-order derivative maximal feature (FDmax) of the CIred edge phenology curves. The relative thresholds of the CIred edge curves were also used to estimate IADAS, in two ways: a single threshold for all of the soybean varieties, and three different relative thresholds for early, middle, and late anthesis varieties, respectively. Finally, this study presented the variations in the IADAS of the same soybean varieties between two different climatic regions and discussed the probable causal factors. The results showed that CIred edge was more suitable for soybean IADAS identification compared with the other investigated indicators because it had no saturation during the whole crop lifespan. Compared with DLF, AGF and FF, SGF provided a better fitting of the CIred edge time-series curves without overfitting problems, although the coefficient of determination (R2) and root mean square error (RMSE) were not the best. The FDmax of the SGF-fitted CIred edge curve (SGF_CIred edge) provided good estimates of the IADAS, with an RMSE and mean average error (MAE) of 3.79 days and 3.00 days, respectively. The SGF-fitted_CIred edge curve can be used to group the soybean varieties into early, middle and late groups. Additionally, the accuracy of the IADAS was improved (RMSE = 3.69 days and MAE = 3.09 days) by using three different relative thresholds (i.e., RT50, RT55, RT60) for the three flowering groups compared to when using a single threshold (RT50). In addition, it was found that the IADAS of the same soybean varieties varied greatly when planted in two different climatic regions due to the genotype–environment interactions. Overall, this study demonstrated that the IADAS of soybean varieties can be identified efficiently and accurately based on UAV remote sensing multispectral time-series data.

Monitoring leaf area index of the sown mixture pasture through UAV multispectral image and texture characteristics

Leaf area index (LAI) is an important phenotypic trait closely related to photosynthesis, respiration, and water utilization. In recent years, unmanned aerial vehicles (UAVs) multispectral capabilities enable the acquisition of spectral information from visible light to near infrared, facilitating vegetation growth monitoring. This study aims to explore the methodology of combining vegetation indices, color indices, texture information, and ecological factors based on UAV multispectral images to enhance the accuracy of the sown mixture pasture LAI estimation. A field experiment involving 13 mixed sowing combinations of alfalfa (Medicago sativa L.), tall fescue (Festuca elata Keng ex E. Alexeev) and plantain (Plantaga lanceolata L.) was conducted out. Multiple linear regression, Bagging algorithm, support vector machine (SVM), random forest algorithm (RF), KNN algorithm, and back propagation neural network (BP) were used to construct the LAI prediction model. The results showed that combining vegetation index (VI) + color index (CI) + normalized difference texture index (NDTI), and ecological factors (EF) could enhance the accuracy of LAI estimation. The sensitive characteristic combinations for alfalfa, tall fescue, and plantain were found to be NDRE (Normalized difference red-edge index) + NGBDI (Normalized green–blue difference index) + (B) MEA-(G) HOM (Blue band Mean - Green band Homogeneity) + DTR (Daily temperature difference), MSR (Modified simple ratio) + NGRDI (Normalized green–red difference index) + (G) COR-(R) VAR (Green band Correlation – Red band Variance) + DTR (Daily temperature difference)), and MSR (Modified simple ratio) + ExG (Excess green) + (G) SEM-(G) MEA (Green band Second-order moment – Green band Mean) + DTR (Daily temperature difference), respectively. RF exhibited superior prediction capability, further enhancing the accuracy of forage LAI prediction. The alfalfa, tall fescue, and plantain obtained coefficient of determination (R2) of 0.83, 0.79 and 0.79, root mean squared error (RMSE) of 0.50, 0.58 and 0.70, and mean absolute error (MAE) of 0.36, 0.45 and 0.55, respectively. These findings provide valuable insights for the estimation of leaf area index of the sown mixture pasture through UAV multispectral images and texture characteristics.

Automatic zone management definition in Mediterranean environment for Triticum durum

AbstractPrecision agriculture (PA) is an agronomic management that allows the parsimonious use of agronomic inputs according to the crop's actual spatiotemporal needs. The most important agronomic practice of PA is the site‐specific management (SSM) of agronomic inputs. To apply SSM, the number and location of homogeneous zones must be defined. Nowadays, many approaches are used to define the number of zones in an arbitrary and constant way, such as high‐, medium‐, and low‐input zones, without a priori statistical analysis. Two combined field experiments were carried out on durum wheat (Triticum turgidum subsp. durum Desf.) in central Italy to agronomically validate an automatic approach aimed at defining the number and position of the homogeneous zones using multispectral images. For both sites, at stem elongation and anthesis, the dry biomass per square meter and Soil Plant Analysis Development (SPAD) readings were measured, while at crop maturity the yield was measured. This approach uses multispectral images as source data and uses the gap statistic index to determine the correct number of zones. The management zones created were well‐fitted with the experimental design of both sites, and the validation was made by the statistical analysis performed on yield data, SPAD, dry biomass, and normalized difference red edge index with an average difference of 40.2%, 28.5%, 57.5%, and 37.4%, respectively, between the management zones. The approach could be scaled by using the multitemporal multispectral images provided by satellite constellation, which enables farms and societies to take advantage of all the economic and environmental benefits of PA.

Unmanned aerial vehicle and proximal sensing of vegetation indices in olive tree (<i>Olea europaea</i>)

Remote and proximal sensing platforms at the service of precision olive growing are bringing new development possibilities to the sector. A proximal sensing platform is close to the vegetation, while a remote sensing platform, such as unmanned aerial vehicle (UAV), is more distant but has the advantage of rapidity to investigate plots. The study aims to compare multispectral and hyperspectral data acquired with remote and proximal sensing platforms. The comparison between the two sensors aims at understanding the different responses their use can provide on a crop, such as olive trees having a complex canopy. The multispectral data were acquired with a DJI multispectral camera mounted on the UAV Phantom 4. Hyperspectral acquisitions were carried out with a FieldSpec® HandHeld 2™ Spectroradiometer in the canopy portions exposed to South, East, West, and North. The multispectral images were processed with Geographic Information System software to extrapolate spectral information for each cardinal direction’s exposure. The three main Vegetation indices were used: normalized difference vegetation index (NDVI), normalized difference red-edge index (NDRE), and modified soil adjusted vegetation index (MSAVI). Multispectral data could describe the total variability of the whole plot differentiating each single plant status. Hyperspectral data were able to describe vegetation conditions more accurately; they appeared to be related to the cardinal exposure. MSAVI, NDVI, and NDRE showed correlation r =0.63**, 0.69**, and 0.74**, respectively, between multispectral and hyperspectral data. South and West exposures showed the best correlations with both platforms.

Impact of in‐season split application of nitrogen on intra‐panicle grain dynamics, grain quality, and vegetative indices that govern nitrogen use efficiency in sorghum

AbstractBackgroundThe correct rate and timing of nitrogen (N) has the potential to improve sorghum productivity through modified grain yield components and quality. The impacts of in‐season split application of N have little documentation.AimAn experiment was conducted to determine the optimum rate and timing of N to relate vegetative indices that govern nitrogen use efficiency and to maximize grain yield and quality under different soil types.MethodsPioneer 86P20 was grown in three environments on two different soil types following a completely randomized block design with nine N application treatments. Treatments included differing N rates applied at critical developmental stages of sorghum (planting, panicle initiation, and booting), accompanied with high temporal aerial phenotyping.ResultsOpportunities to increase grain protein content while using split N applications were observed, with panicle initiation identified as a critical developmental stage. In‐season split application of N enhances grain yield under low soil mineral N. Split application of 31 kg N ha−1 each at the time of planting, panicle initiation, and booting emerged as optimum N treatment to increase protein content in sorghum. Vegetative indices, that is, normalized difference vegetation index and normalized difference red edge index are capable of predicting grain yield and protein content, respectively. Intra‐panicle grain numbers and weights were altered significantly at different portions within panicles, with an opportunity to enhance yield potential at the bottom portion. The strong stay‐green trait in this hybrid locked a large proportion of nitrogen in the leaves, which warrants the need for balancing stay‐green and senescence in sorghum improvement programs.ConclusionsFindings highlight that in grain sorghum remobilization of residual leaf N into grain is a target to increase yield and grain quality. An optimized stay‐green trait balanced with senescence is recommended for enhancing sorghum yield potential.

How similar is the zoning of different vegetation indices: Defining the optimal framework for monitoring grapevines’ growth within vigorous vineyards

The spatial patterns of grapevines’ vigor across vigorous vineyards were assessed through distinct vegetation indices (VI) for comparison purposes. This survey also aids the selection of the most sensitive VI to monitor the vineyards during a given optimal stage of the last period of the growing season. The canopy reflectance (ρ) of grapevines cv. Cabernet Franc and Cabernet Sauvignon (Vitis vinifera L.) was measured throughout their berry development and ripening stages. Georeferenced data of ρ at the red (680 nm), red edge (730 nm), and near-infrared (780 nm) wavebands were taken using a hand-held active sensor to compute the normalized difference red edge index (NDRE), and the normalized difference vegetation index (NDVI). Spatial predictions of both VI were estimated by geostatistical interpolation technique (kriging) and then categorized into homogeneous zones (HZ) through the Jenks natural breaks optimization method to delimit low and high vigor regions. Statistics of the inter-rater reliability between each VI zoning were calculated afterward. The relative dispersion of data around their mean was assumed as a criterion to assess the sensibility of each VI in detecting vigor variability under a given condition. The most appropriate stage to monitor the vineyards was determined based on the peak of vegetative growth inferred from the values of the VI we presumed as most sensitive. Differences between the HZ defined from the best combination of VI and stage in terms of yield, cane weight, and crop load were also examined. We demonstrated that NDRE was more sensitive than NDVI in detecting the vigor variability of high-density vegetation due to the relative dispersion of its datasets and the saturation effect of NDVI at the late stages of the grapevine growing season. The lowest and higher agreement between the VI zoning was usually detected at the berry touch and berry softening growth stages, respectively. Although there were distinct levels of concordance between NDRE and NDVI maps, their overall agreement was higher than 70% in most surveys. The disagreement in quantity or spatial allocation of vigor categories provided by each VI relied on the vineyard, growth stage, and growing season. Nevertheless, the early definition of HZ from NDRE monitoring carried out at veraison could be suitable to identify differences in cane weight at pruning, with a moderate effect size.

Identification of new cold tolerant Zoysia grass species using high-resolution RGB and multi-spectral imaging

Zoysia grass (Zoysia spp.) is the most widely used warm-season turf grass in Korea due to its durability and resistance to environmental stresses. To develop new longer-period greenness cultivars, it is essential to screen germplasm which maintains the greenness at a lower temperature. Conventional methods are time-consuming, laborious, and subjective. Therefore, in this study, we demonstrate an objective and efficient method to screen maintaining longer greenness germplasm using RGB and multispectral images. From August to December, time-series data were acquired and we calculated green cover percentage (GCP), Normalized Difference Vegetation Index (NDVI), Normalized Difference Red Edge Index (NDRE), Soil-adjusted Vegetation Index (SAVI), and Enhanced Vegetation Index (EVI) values of germplasm from RGB and multispectral images by applying vegetation indexs. The result showed significant differences in GCP, NDVI, NDRE, SAVI, and EVI among germplasm (p < 0.05). The GCP, which evaluated the quantity of greenness by counting pixels of the green area from RGB images, exhibited maintenance of greenness over 90% for August and September but, sharply decrease from October. The study found significant differences in GCP and NDVI among germplasm. san208 exhibiting over 90% GCP and high NDVI values during 153 days. In addition, we also conducted assessments using various vegetation indexes, namely NDRE, SAVI, and EVI. san208 exhibited NDRE levels exceeding 3% throughout this period. As for SAVI, it initially started at approximately 38% and gradually decreased to around 4% over the course of these days. Furthermore, for the month of August, it recorded approximately 6%, but experienced a decline from about 9% to 1% between September and October. The complementary use of both indicators could be an efficient method for objectively assessing the greenness of turf both quantitatively and qualitatively.