Normal Pulse Research Articles

Selective depletion of kisspeptin neurons in the hypothalamic arcuate nucleus in early juvenile life reduces pubertal LH secretion and delays puberty onset in mice.

Puberty is the critical developmental transition to reproductive capability driven by the activation of gonadotropin-releasing hormone (GnRH) neurons. The complex neural mechanisms underlying pubertal activation of GnRH secretion still remain unknown, yet likely include kisspeptin neurons. However, kisspeptin neurons reside in several hypothalamic areas and the specific kisspeptin population timing pubertal onset remains undetermined. To investigate this, we strategically capitalized on the differential ontological expression of the Kiss1 gene in different hypothalamic nuclei to selectively ablate just arcuate kisspeptin neurons (aka KNDy neurons) during the early juvenile period, well before puberty, while sparing RP3V kisspeptin neurons. Both male and female transgenic mice with a majority of their KNDy neurons ablated (KNDyABL) by diphtheria toxin treatment in juvenile life demonstrated significantly delayed puberty onset and lower peripubertal LH secretion than controls. In adulthood, KNDyABL mice demonstrated normal invivo LH pulse frequency with lower basal and peak LH levels, suggesting that only a small subset of KNDy neurons is sufficient for normal GnRH pulse timing but more KNDy cells are needed to secrete normal LH concentrations. Unlike prior KNDy ablation studies in rats, there was no alteration in the occurrence or magnitude of estradiol-induced LH surges in KNDyABL female mice, indicating that a complete KNDy neuronal population is not essential for normal LH surge generation. This study teases apart the contributions of different kisspeptin neural populations to the control of puberty onset, demonstrating that a majority of KNDy neurons in the arcuate nucleus are necessary for the proper timing of puberty in both sexes.

6768 A Rare Presentation of Primary Adrenal Insufficiency with Hypertension & Hypokalemia

Abstract Disclosure: A.M. San Hernandez: None. R. Alshantti: None. G. Espinoza Mercado: None. J.L. San Hernandez: None. Introduction: Adrenal Insufficiency is caused by the failure of the adrenal cortex to produce glucocorticoids +/- mineralocorticoids. This can be due to a defect in the adrenal cortex, or in the hypothalamic pituitary axis. The clinical manifestations are usually non-specific, ranging from mild vague symptoms to life threatening crisis. Therefore, diagnosis requires a high level of suspicion by providers. Unusual presentations of primary adrenal insufficiency (PAI) with hypertension and hypokalemia have been rarely described in the literature. We present a rare case of PAI with hypertension and hypokalemia. Case Presentation: A 68 years old female with a medical history of rheumatoid arthritis, hypothyroidism on Levothyroxine, chronic transaminitis, and bicytopenia presented to the hospital with a 6 weeks history of generalized weakness, loss of appetite, weight loss, and dizziness. Patient denied any diuretic or steroid use within the last 2 years. On arrival, she was afebrile, hypertensive (168/84mmHg), with normal oxygenation and pulse. Physical examination was unremarkable with normal volume status. Initial laboratory results showed low sodium 116 mEq/L, low serum osmolality 247 MEQ/kg, normal potassium 3.6 mEq/L, Creatinine 0.3 mg/dL & BUN 9 mg/dL. She was initially started on IVF normal saline. Further workup revealed normal TSH & FT4 levels. Urine study showed urine osmolality (477 MOSM/KG), & urine sodium (127 mEq/L). Given these findings, SIADH was suspected and fluid restriction was started. Sodium level continued to be low with minimal improvement to 120 mEq/L, potassium levels also dropped (3.3-3.2 mEq/L), requiring daily replacement. BP remained elevated ranging from 150s to 160s systolic and 80s to 90s diastolic. Hypertension was controlled with her home medication Amlodipine. Although atypical presentation with hypertension & hypokalemia, the diagnosis of adrenal insufficiency was considered. Laboratory evaluation showed low morning cortisol 5.2 ug/dL, with album level 3.5 g/dL. Cosyntropin stimulation test showed 1.3 ug/dL (at baseline), 9.9 (30 min post administration), and 14.4 (1 hour post administration); results confirming PAI. ACTH, Renin, Aldosterone, & DHEAS levels were normal. CT adrenal was normal. Patient was started on hydrocortisone; some of her symptoms & Na level improved. Further investigation of the etiology of PAI and possible hyperaldosteronism is pending outpatient follow up. Conclusion: Adrenal insufficiency is a life threatening condition with serious consequences if diagnosis is missed. It manifests by a wide spectrum of symptoms and signs, some can be masked by the presence of other comorbid conditions leading to atypical presentations. Our case highlights the importance of maintaining a high level of suspicion of adrenal insufficiency in patients with atypical presentation, including those with hypertension & hypokalemia. Presentation: 6/3/2024

Nitrogen functionalized biomass derived mesoporous carbon nanomaterials for electrochemical detection of lead (II) ions

This study has explored the sustainable solution after designing an economical metal-free biomass-derived nanocarbon for the selective sensing of lead. The nitrogen and sulfur-rich mesoporous nanocarbon is designed through a facile hydrothermal-assisted thermal annealing method. The high-temperature treatment gave nanocarbon unique carbon dot decorated layered morphology, while nitrogen and sulfur precursor thiourea and melamine strengthened the nanomaterial stability, sensitivity, and selectivity toward lead metal ions. The high specific surface area of mesoporous nanocarbon viz., 1671.93 m2/g with the pore width and pore volume of 2.02 nm and 0.476 cm3/g has enhanced the conductivity of as-synthesized sensor, which helps in increasing sensitivity toward lead. The high conductivity was also confirmed through cyclic voltammetry, where an 80 % increment in current was observed in the case of the modified electrode when compared with bare GCE. The differential pulse normal voltammetry and differential pulse anodic stripping voltammetry were performed to calculate the detection limit, where an excellent detection limit of 22 nM was obtained from the DPASV technique. Moreover, the nanomaterial was also tested for detecting lead in tap water. The as-synthesized nanocomposite is highly efficient and selective for the detection of lead. This study will motivate the researchers to engineer sustainable and efficient devices for sensing metal pollutants.

Pulse Rate of Jogging Two Kilometers of Constant Pace Running

The study aims to obtain data on the average pulse rate before and after running 5 laps to determine the level of dehydration of a person. The pulse is a form of the heart's ability, which is located in a certain area. The pulse rate can be used as a reference to determine heart rate, rhythm, and heart strength. The method used is to calculate the normal pulse rate, then the sample runs 5 laps of the soccer field, after the test the sample returns to calculate the pulse rate by holding the artery area and counting it for 1 minute while standing and calm. From the research conducted on a sample of 16 active PKO students, there are women and men, from the average data obtained, the results of the pulse rate is higher after running 5 laps, namely 168 times per minute, compared to before running 5 laps, with an average of 102 times per minute.

Effects of Energy Drink Consumption on Specific Cardiovascular and Psycho-Behavioral Parameters Among Medical Students at the University of Zakho.

Background The consumption of energy drinks (EDs) among university students has become a prevalent trend, raising concerns about potential health risks. EDs are beveragescontaining various ingredients, most notably caffeine, taurine, and vitamins, that are consumed by the general public and athletes to reduce exhaustion, boost energy, and improve performance. Objectives This study aimed to investigate the prevalence of ED consumption and evaluate the effects of EDuse on cardiovascular health and behavioral patterns in a sample of students atthe University of Zakho. Methods This cross-sectional study involved a sample of 438 medical students aged 18-25 at the University of Zakho. Data were collected using a closed-ended questionnaire assessing socio-demographic characteristics, ED consumption habits, awareness of ED-related health risks, and cardiovascular measures such as blood pressure, pulse rate, presence of chest pain, and palpitation. Results The prevalence of consuming caffeine-content EDs was surprisingly high (70%, n=307) among the University of Zakho's medical students. Further categorization revealed that the prevalence was 42% (n=187) among low-frequency drinkers, 22.2% (n=95) among those who drank once a day, and 5.8% (n=25) among the frequent group. A higher percentage of ED drinkers (30%, n=92) developed tachycardia compared to students with a normal pulse (19.2%, n=59). Additionally, ED drinkers had significantly higher rates of elevated blood pressure (56.4%, n=173), palpitations (63.1%, n=194), and chest discomfort (73.2%, n=225) compared to non-drinkers (p <0.0001). Concerning behavioral characteristics, ED drinkers had a significantly higher rate of fatigability (79.3%, n=243) compared to non-drinkers (p <0.0001). They were more likely to experience somnolence (60.8%, n=187) compared to non-drinkers (p <0.05). Furthermore, the percentage of students with aggressive behavior was substantially higher (p <0.001) among ED drinkers (86.2%, n=265). Conclusion The findings collectively highlight the significant negative impact of energy drink consumption on health in general and cardiovascular and behavioral variables in particular. It also underscores the need for public health strategies and campus interventions to reduce ED consumption and raise awareness about their potential risks.

Examining the Light Heart Mobile Device App for Assessing Human Pulse Interval and Heart Rate Variability: Validation Study.

Pulse interval is a biomarker of psychological and physiological health. Pulse interval can now be assessed using mobile phone apps, which expands researchers' ability to assess pulse interval in the real world. Prior to implementation, measurement accuracy should be established. This investigation evaluated the validity of the Light Heart mobile app to measure pulse interval and pulse rate variability in healthy young adults. Validity was assessed by comparing the pulse interval and SD of normal pulse intervals obtained by Light Heart to the gold standard, electrocardiogram (ECG), in 14 young healthy individuals (mean age 24, SD 5 years; n=9, 64% female) in a seated posture. Mean pulse interval (Light Heart: 859, SD 113 ms; ECG: 857, SD 112 ms) demonstrated a strong positive linear correlation (r=0.99; P<.001) and strong agreement (intraclass correlation coefficient=1.00, 95% CI 0.99-1.00) between techniques. The Bland-Altman plot demonstrated good agreement for the mean pulse interval measured with Light Heart and ECG with evidence of fixed bias (-1.56, SD 1.86; 95% CI -5.2 to 2.1 ms), suggesting that Light Heart overestimates pulse interval by a small margin. When Bland-Altman plots were constructed for each participant's beat-by-beat pulse interval data, all participants demonstrated strong agreement between Light Heart and ECG with no evidence of fixed bias between measures. Heart rate variability, assessed by SD of normal pulse intervals, demonstrated strong agreement between techniques (Light Heart: mean 73, SD 23 ms; ECG: mean 73, SD 22 ms; r=0.99; P<.001; intraclass correlation coefficient=0.99, 95% CI 0.97-1.00). This study provides evidence to suggest that the Light Heart mobile app provides valid measures of pulse interval and heart rate variability in healthy young adults.

Kinetics of Charge Reversal in the Double Layer Studied with an Electrochemical Quartz Crystal Microbalance (EQCM-D)

Normal pulse voltammetry on inert electrolytes was combined with a new electrochemical quartz crystal microbalance (EQCM) with millisecond time resolution, which allows to probe the kinetics of charge reversal in the diffuse double layer (DL) after a step in electrode potential. The QCM responds to the charge reversal because the local viscosity-density product depends on the type and the concentration of the ions. The time resolution was improved to 1 ms using multifrequency lockin amplification, which allows to study kinetic effects in dynamic electrochemistry, which were previously not accessible with gravimetry. The noise was lowered to less than 10 mHz by modulating the electrode potential and averaging Δf and ΔΓ over many modulation cycles.The overtone scaling was found to be the same as in gravimetry (-Δf/n » const. and ΔΓ/n < -Δf/n with n the overtone order), which, however, is not caused by a deposition of a rigid film in the Sauerbrey sense. A Sauerbrey-type QCM response is also caused by changes in Newtonian viscosity inside a layer with a thickness far below the penetration depth of the shear wave (such as the double layer).The frequency response in the electrolytes studied correlated well with the viscosity B-coefficients of the respective ions as employed in the Dole-Jones equation. The time constants inferred from gravimetry were similar to the time constants (the RC-times) from electrochemical impedance spectroscopy (EIS). There were small differences, which presumably go back to slow rearrangements inside the Helmholtz layer, which are not seen in EIS.The QCM response to double layer recharging in inert electrolytes is of great importance for the interpretation of QCM results on processes involving Faradayic charge transfer. Such a response is omnipresent as a background and might be misinterpreted as a gravimetric signal.[1] References[1] Leppin, C.; Peschel, A.; Meyer, F. S.; Langhoff, A.; Johannsmann, D. Kinetics of Viscoelasticity in the Electric Double Layer Following Steps in the Electrode Potential Studied by a Fast Electrochemical Quartz Crystal Microbalance (EQCM). Analyst 2021, 146 (7), 2160–2171. Caption: Shifts in frequency and bandwidth in response to potential steps (left). The gravimetric signal followed the steps with a delay of a few milliseconds. The amplitude and the response time depend on cation type (right). The counter ion was NO3 -. Both the amplitude and the response time correlate well with the viscosity B-coefficient. Figure 1

Diurnal fluctuations in biochemical parameters related to calcium homeostasis – the Bispebjerg study of diurnal variations

Purpose This aim of this study was to assess the possible association between diurnal oscillations and biochemical markers associated with calcium homeostasis. This included the markers parathyroid hormone (PTH), total calcium, total alkaline phosphatase, phosphate, and 25-hydroxyvitamin D (25-OH-D). By examining the influence of circadian rhythms on these parameters, the study aimed to deepen the understanding of calcium metabolism dynamics and its clinical implications. Patients and methods Blood samples from 24 Caucasian male volunteers aged 20 to 40 (mean age 26) with normal pulse, blood pressure, and BMI were analyzed for biochemical markers related to calcium homeostasis. Data was obtained from the Bispebjerg study of diurnal variations. Blood samples were collected every three hours over a 24-hour period. Patients were fasting from 22:00 to 09:00. The participants spent 24 h in the hospital ward, receiving regular meals and engaging in low-intensity activities. They experienced 15 h of daylight and 9 h of complete darkness during sleep. Diurnal oscillations were analyzed using cosinor analysis with statistical significance set at p < 0.05. Results Total calcium, phosphate, and PTH exhibited significant diurnal variations. Total calcium and PTH were inversely synchronized while PTH and phosphate oscillated in synchronization. The three parameters showed relatively large amplitude/reference range ratios from 25.4% to 41.5%. Conclusion This study found notable fluctuations in total calcium, phosphate, and PTH levels over a 24-hour cycle, while 25-OH-D and total alkaline phosphatase remained consistent. It highlights the importance of considering sampling times for total calcium, PTH, and phosphate in clinical settings.

Au24Cd Nanoenzyme Coating for Enhancing Electrochemical Sensing Performance of Metal Wire Microelectrodes.

Dopamine (DA), ascorbic acid (AA), and uric acid (UA) are crucial neurochemicals, and their abnormal levels are involved in various neurological disorders. While electrodes for their detection have been developed, achieving the sensitivity required for in vivo applications remains a challenge. In this study, we proposed a synthetic Au24Cd nanoenzyme (ACNE) that significantly enhanced the electrochemical performance of metal electrodes. ACNE-modified electrodes demonstrated a remarkable 10-fold reduction in impedance compared to silver microelectrodes. Furthermore, we validated their excellent electrocatalytic activity and sensitivity using five electrochemical detection methods, including cyclic voltammetry, differential pulse voltammetry, square-wave pulse voltammetry, normal pulse voltammetry, and linear scanning voltammetry. Importantly, the stability of gold microelectrodes (Au MEs) modified with ACNEs was significantly improved, exhibiting a 30-fold enhancement compared to Au MEs. This improved performance suggests that ACNE functionalization holds great promise for developing micro-biosensors with enhanced sensitivity and stability for detecting small molecules.

Alignment of lyotropic liquid crystals using magnetic nanoparticles improves ionic transport through built-in peptide ion channels

Hypothesis: We hypothesize that simultaneous incorporation of ion channel peptides (in this case, potassium channel as a model) and hydrophobic magnetite Fe3O4 nanoparticles (hFe3O4NPs) within lipidic hexagonal mesophases, and aligning them using an external magnetic field can significantly enhance ion transport through lipid membranes.Experiments: In this study, we successfully characterized the incorporation of gramicidin membrane ion channels and hFe3O4NPs in the lipidic hexagonal structure using SAXS and cryo-TEM methods. Additionally, we thoroughly investigated the conductive characteristics of freestanding films of lipidic hexagonal mesophases, both with and without gramicidin potassium channels, utilizing a range of electrochemical techniques, including impedance spectroscopy, normal pulse voltammetry, and chronoamperometry.Findings: Our research reveals a state-of-the-art breakthrough in enhancing ion transport in lyotropic liquid crystals as matrices for integral proteins and peptides. We demonstrate the remarkable efficacy of membranes composed of hexagonal lipid mesophases embedded with K+ transporting peptides. This enhancement is achieved through doping with hFe3O4NPs and exposure to a magnetic field. We investigate the intricate interplay between the conductive properties of the lipidic hexagonal structure, hFe3O4NPs, gramicidin incorporation, and the influence of Ca2+ on K+ channels. Furthermore, our study unveils a new direction in ion channel studies and biomimetic membrane investigations, presenting a versatile model for biomimetic membranes with unprecedented ion transport capabilities under an appropriately oriented magnetic field. These findings hold promise for advancing membrane technology and various biotechnological and biomedical applications of membrane proteins.

Intracranial pressure following surgery of an unruptured intracranial aneurysm—a model for normal intracranial pressure in humans

ObjectiveOptimizing the treatment of several neurosurgical and neurological disorders relies on knowledge of the intracranial pressure (ICP). However, exploration of normal ICP and intracranial pressure pulse wave amplitude (PWA) values in healthy individuals poses ethical challenges, and thus the current documentation remains scarce. This study explores ICP and PWA values for healthy adults without intracranial pathology expected to influence ICP.MethodsAdult patients (age > 18 years) undergoing surgery for an unruptured intracranial aneurysm without any other neurological co-morbidities were included. Patients had a telemetric ICP sensor inserted, and ICP was measured in four different positions: supine, lateral recumbent, standing upright, and 45-degree sitting, at day 1, 14, 30, and 90 following the surgery.ResultsICP in each position did not change with time after surgery. Median ICP was 6.7 mmHg and median PWA 2.1 mmHg in the supine position, while in the upright standing position median ICP was − 3.4 mmHg and median PWA was 1.9 mmHg. After standardization of the measurements from the transducer site to the external acoustic meatus, the median ICPmidbrain was 8.3 mmHg in the supine position and 1.2 mmHg in the upright standing position.ConclusionOur study provides insights into normal ICP dynamics in healthy adults following a uncomplicated surgery for an unruptured aneurysm. These results suggest a slightly wider normal reference range for invasive intracranial pressure than previously suggested, and present the first normal values for PWA in different positions. Further studies are, however, essential to enhance our understanding of normal ICP.Trial registration The study was preregistered at www.clinicaltrials.gov (NCT03594136) (11 July 2018)

A pulse selection algorithm for SiPM-coupled CLYC detectors

SiPM-coupled Cs2LiYCl6:Ce3+ (CLYC) detectors are widely used for detecting neutrons and gamma rays in mixed radiation environments. Intensities and energies are obtained from the output pulses of the detector, including normal and anomalous pulses. Since anomalous pulses can negatively affect measurement accuracy, this study designs a pulse selection algorithm to improve them. This algorithm calculates the ratio of the mean to the standard deviation of an output pulse in a particular time window and categorizes it as normal or anomalous. The algorithm also uses the difference in ratio value between neutrons and gamma rays for pulse shape discrimination. In an experiment using a SiPM-coupled CLYC detector, 200,000 sets of pulses were obtained for 137Cs and Am-Be sources. The results indicate that this method can reject more than 94.2% of anomalous pulses, improving the energy resolution of 137Cs source from 8.9% @ 662 keV to 8.4% @ 662 keV. The figure-of-merit (FOM) of pulse shape discrimination is 1.23 for the Am-Be source, and this method can select specific pulse types based on the pulse-ratio value. In addition, the method is suitable for all pulse-mode detectors.

Dwarf Pulses of 10 Pulsars Detected by FAST

How pulsars radiate is a long-standing problem. Detailed polarization measurements of individual pulses shed light on currently unknown emission processes. Recently, based on supersensitive observations, dwarf pulses have been recognized as weak narrow pulses often appearing during the nulling state. In this study, we report the detection of dwarf pulses from 10 pulsars, PSRs B0525+21, B1237+25, J1538+2345, J1824−0127, J1851−0053, B1901+10, J1939+10, B1944+17, B2000+40, and J2112+4058, based on observations conducted with the Five-hundred-meter Aperture Spherical radio Telescope. Dwarf pulses of five pulsars are clearly discernible in the two-dimensional distribution of pulse intensity and pulse width. For the other five pulsars, PSRs J1538+2345, J1824−0127, J1939+10, B2000+40, and J2112+4058, only a few dwarf pulses are detected from pulse stacks. The dwarf pulses can emerge in both cone and core emission components for PSR B1237+25, and the polarization angles of these dwarf pulses are mostly in the orthogonal polarization mode of normal pulses for PSR B1944+17. In general, pulsars with detected dwarf pulses tend to be located within the “death valley” region of the distribution of pulsar periods and period derivatives.

Field-free orientation of 7LiH steered by a few-cycle nonlinearly chirped pulse

We demonstrate theoretically that an efficient field-free molecular orientation of 7LiH molecule steered by a few-cycle nonlinearly chirped laser pulse with frequency in the terahertz regime can be achieved. The frequency modulation parameter β plays an important role in the molecular orientation, the maximum molecular orientation |〈cosθ〉|max is 0.848 and the orientation duration is 150 fs when β=7.25. In the range of electric-field peak intensity E0=[0.1,4.0] MV/cm, we can find the maximum orientation degree in both positive and negative directions. Compared to the scheme steered by a normal laser pulse, we can find that a more efficient molecular orientation and a smaller difference in population between the adjacent states can be realized by using the nonlinearly chirped laser pulse under the same near-single-period conditions. In addition, at the same temperature, a higher molecular orientation and a smaller decrease rate of orientation with temperature can be obtained by the nonlinearly chirped laser pulse.

Description of Hemodynamic Changes in Section Caesarea Patients with Spina; Anesthesia in Sijunjung Regional General Hospital

Background: Every anesthetic action must Background: Every anesthetic action must pay attention to the patient's condition because this anesthetic action causes effects on all body systems, including hemodynamic changes in the patient's body. To see if there are hemodynamic changes that occur, blood pressure and pulse monitoring can be done during the Sectio Caesarea surgery process. Purpose: to know the description of hemodynamic changes in Sectio Caesaria patients with spinal anesthesia at Sijunjung Regional General Hospital. Methods: The type of research used is observational research with descriptive research type. The population in this study were all patients with cesarean section with spinal anesthesia at the Sijunjung Regional General Hospital in July - August with a sample size of 20 patients. Research instruments used ECG monitors and observation sheets using descriptive data analysis. Findings: blood pressure and pulse of respondents before spinal anesthesia were seen entirely, namely 20 respondents (100%) with normal blood pressure and pulse and after spinal anesthesia as many as 14 people (70%) experienced hypotension and 6 respondents (30%) with normal blood pressure. While the pulse rate in respondents after spinal anesthesia was 11 people (50%) experiencing tachycardia and 9 people (45%) with a normal pulse. Conclusion: then it means that there is a picture of hemodynamic changes in sectio caesaria patients with spinal anesthesia at the Sijunjung Regional General Hospital.

A Cacophony of Echoes from Daily Monitoring of the Crab Pulsar at Jodrell Bank

Using archival data from the 42-foot telescope at the Jodrell Bank Observatory, we produce daily stacks of aligned giant pulses for the Crab pulsar, to study changes to the daily profiles between 2012 April and 2016 December. From these, we identify echoes, where intervening material away from the line of sight causes pulsed emission to be redirected toward the observer, with delay corresponding to the increased distance of travel, resulting in additional profile components. These observations show that such echoes may be far more common than implied by the previous rate of detections. All the observed echoes are consistent with approaching zero delay at their closest approach to the normal giant pulse emission. This indicates that the structures responsible for producing these events must be highly anisotropic, with typical lengths greater than ∼4 au, typical widths on the sky of ∼0.1 au, and typical depths of ∼5 au, given the previously observed electron densities of the nebular filaments, on the order of 1000 cm−3. This suggests that these inhomogeneities are likely to be offshoot substructure from the larger nebular filaments of the Crab nebula.

SURGICAL MANAGEMENT OF GRANULOMA IN A 5-YEAR-OLD UDA EWE

Granuloma is a well-known inflammatory condition in veterinary and human medicine. It is a chronic inflammatory reaction triggered by a wide variety of persistent agents, characterized by the recruitment and activation of macrophages, occasionally presenting areas of central necrosis. The aim of the present report is to provide information regarding the manifestation, cytology, histology and management of granuloma in sheep. A five-year-old Uda ewe was presented to the Veterinary Teaching Hospital, University of Jos in September, 2023, with a large mass around the neck, extending towards the jugular furrow. Following cytological examination of the mass, surgical excision was undertaken under mild sedation (2% Xylazine) and local anaesthesia (1% Lidocaine hydrochloride). The mass was subjected to histopathological examination, while routine wound cleansing and care was instituted with post-operative administration of analgesic, anti-inflammatory agent, antibiotics, multivitamins and tetanus toxoid intramuscularly. Histopathological findings comprised of masses having necrotic centre with sulfur granules, area of macrophages, lymphocyte, plasma cell and zones of fibro-connective tissue with evidence of neo-vascularization and perivascular cuffing supporting the diagnosis of granuloma. Follow-up re-examinations of the Ewe were undertaken every other day with stitches removed on day 12 post-operation following complete apposition of the wound edges. After 14 days of management, the ewe was discharged following normal rectal temperature (39.0ºC), pulse rates (80.0 beats/minute) and respiratory rates (28.0 cycles/minute). Therefore, cases of granuloma could be satisfactorily managed by complete surgical excision with thorough post-operative care.

Traditional medicine of three regions of Russia and the use of information technologies to investigate it

The paper presents experience of investigating the traditional medicine for the three regions of Russia – Buryatia, Kalmykia and Tuva — that has been kind of folk tradition here for centuries. This is Tibetan medicine that is the cultural and historical heir of the Indian medical tradition using some elements of the Chinese medicine. The paper gives information on the problem of objectifying and automating the Tibetan diagnostic methods (inspection, palpation, questioning) using information technologies. This research has been carried out in two directions: objectification and automation of pulse diagnostics by the development of the Computer Pulse Diagnostic System and the development of the Expert Diagnostic System for questioning and inspection of a patient. On the basis of the assessments of the experts (specialists in pulse diagnostics) and mathematically detected diagnostic signs of pulse waves, statistical models of normal and pathological pulses (pulses of «hot» and «cold» diseases) have been developed. A catalogue of pulses is being compiled. This catalogue consists of classes of computer recorded pulse signals that correspond to Tibetan nosology. The expanded and differentiated catalog of pulses and the pulse wave pattern recognition program designed to compare measured pulses and reference pulses will make the basis for combining the diagnostic results by three diagnostic methods, which will lead to the creation of an Integral Computer Diagnostic System designed for diagnosing diseases and providing recommendations for health correction in accordance with the traditions of Tibetan medicine.

The LATIHAN SENAM AEROBIK DAN PENGUKURAN TEKANAN DARAH DAN DENYUT NADI WARGA RT 09 RW 09 JATISARI JATIASIH KOTA BEKASI JAWA BARAT

Physical fitness is a condition that must be maintained by someone who wants health. With excellent health, you can carry out work activities to earn a living. The benefit of this community service is doing aerobic exercise, especially for body fitness, and measuring body fitness levels. Indicators of body fitness are normal blood pressure and pulse. In this community service activity, aerobic exercise, blood pressure, and pulse measurements were carried out. Almost all residents attended the aerobic exercise activities, while the measurement of blood pressure and pulse was based on a sampling technique using purposive sampling. This sampling technique is based on inclusion and exclusion criteria. The statistical analysis used is descriptive, that is, it only looks at the description of the condition of blood pressure and pulse before and after aerobic exercise. These results show that after aerobic exercise, blood pressure and pulse measurements were more stable than before exercise.

Building design features and wind effects on rabbits in cages

Rearing rabbits in the tropics has been discovered to be a lucrative alternative to providing white meat to the populace. While red meat is available, evidence reveals that they are more expensive; hence, the need to increase rabbit production especially as they can be conveniently reared at home backyards and as they can easily convert family’s leftover foods into meat. However, rabbits’ comfortability during rearing cannot be compromised, thus the research was borne out to investigate their welfare through their respiratory and pulse rates when reared in different housing designs. Buildings were constructed: width = 1.2 m; length = 4.8 m; height = 1.2 m, all at 0.5 m height from the ground, each with pens in accordance with desired building openings and building orientations, all were for a pen per doe. Factors considered were the season of the year at two levels: dry and rainy seasons; building orientations at three levels: 0o, 45º, and 90º to the prevailing wind’s direction and ventilation side openings: four levels of 50 %, 60 %, 70%, and 80 %, all replicated thrice to be 2 x 3 x 4 x 3 factorial design. Measured were pulse and respiratory rates at 10.00 h and 14.00 h daily. Interactions between different openings, orientations and seasons were significant at p≤0.01 on the pulse rate of does. The range of wind speed was between 3.61 and 5.04 km/h, this falls within the thermo-comfort range for does. There were higher R2 values, which depicted high correlations between the animals’ and the wind directions or wind speeds. The low wind speeds recorded cumulatively resulted in normal pulse rates and respiratory rates in does at all orientations. The effects of all building designs did not change the normalcy in both respiratory and pulse rates in the rabbits’ lives in the reared environment.