Non-urban Sites Research Articles

Effects of urbanization on orchid bee diversity and orchid pollination: From neotropical cloud forests to urban cores

Urbanization is a significant driver of land use change, profoundly impacting biodiversity and ecosystem services worldwide. However, its effects in the tropics, which host some of the planet's highest biodiversity, remain inadequately understood. Orchid bees (Apidae: Euglossini) are key pollinators in Neotropical ecosystems, playing crucial roles in maintaining floral diversity and reproductive success of orchids and other plant families. Yet, little is known about how urbanization influences their diversity and pollination. In this study, we analyzed the diversity and composition or orchid bee communities along an urbanization gradient which extends from the city center to the surrounding cloud forests, which bear high orchid endemism while being highly threatened. Along the same gradient, we further evaluated pollination of a model native orchid, Gongora galeata, which is exclusively pollinated by the bee Euglossa obrima. As expected, increasing urbanization led to a decrease in orchid bee diversity, as well as a clear separation in species composition between urban and non-urban sites and a reduction in G. galeata pollination (i.e. fruit production). However, contrary to our expectations, orchid pollination also decreased with environmental heterogeneity and the abundance of its specific pollinator. Despite urban areas still hosting orchid bee species, our results reveal clear negative effects of urbanization not only on diversity, but also on the ecosystem function of a highly threatened group of bees. This study highlights the importance of considering local factors of urban landscapes for preserving not only biodiversity, but also fundamental ecological processes in cities.

Wind-driven emissions of coarse-mode particles in an urban environment

Abstract. Quantifying surface–atmosphere exchange rates of particles is important for understanding the role of suspended particulate matter in radiative transfer, clouds, precipitation, and climate change. Emissions of coarse-mode particles with a diameter greater than 0.5 µm provide giant cloud condensation nuclei and ice nuclei. These emissions are critical for understanding the evolution of cloud microphysical properties yet remain poorly understood. Here we introduce a new method that uses lidar retrievals of the elastic backscatter and Doppler velocity to obtain surface number emissions of particles with a diameter greater than 0.53 µm. The technique is applied to study particle number fluxes over a 2-month period from 1 June to 10 August 2022 during the TRACER campaign at an urban site near Houston, TX, USA. We found that all the observed fluxes were positive (upwards), indicating particle emission from the surface. The fluxes followed a diurnal pattern and peaked near noon local time. Flux intensity varied through the 2 months with multi-day periods of strong fluxes and multi-day periods of weak fluxes. Emission particle number fluxes peaked near ∼ 100 cm−2 s−1. The daily averaged emission fluxes correlated with friction velocity and were anticorrelated with surface relative humidity. The emission flux can be parameterized as F= 3000 u*4, where u* is the friction velocity in m s−1 and the emission flux F is in cm−2 s−1. The u* dependence is consistent with emission from wind-driven erosion. Estimated values for the mass flux are in the lower range of literature values from non-urban sites. These results demonstrate that urban environments may play an important role in supplying coarse-mode particles to the boundary layer. We anticipate that quantification of these emissions will help constrain aerosol–cloud interaction models that use prognostic aerosol schemes.

Comparative Health Status of Women of Childbearing Age in Urban and Non-urban Communities in Malaysia

Background Women’s health and access to healthcare can be affected by diverse factors such as affluence, cultural norms and availability of healthcare resources. In this study, we compared health status parameters among women of childbearing age from different ethnic groups living in urban and non-urban communities in Malaysia. Methods Women under the age of 45-years in urban and non-urban areas of Malaysia were invited to participate in this study. In total, 1500 women were approached, and of these 1435 agreed to participate and met our inclusion criteria. Information about personal healthcare, communication with healthcare practitioners, sources of health information, health concerns, weight management, as well as socio-demographic characteristics were obtained using a questionnaire which was adapted from the Women’s Health National Survey 2016 of Australia. Results Women of Indian descent made up a higher proportion of respondents in non-urban areas. Women in non-urban areas were less likely to have a higher-level education and belonged to households with a lower annual income. Non-urban respondents were also more likely to be married and to have more children. Women from non-urban locations were less likely to be overweight or exhibit sedentary behaviors. Women from non-urban sites were more interested in improving their health status, more likely to attend routine health checks, and less likely to use dietary supplements. Conclusion Women from non-urban areas exhibit healthier behaviours than their urban counterparts and have a lower prevalence of obesity. This is despite poorer access to healthcare provision in rural communities.

Assessing hydroxylated polycyclic aromatic hydrocarbon (OHPAH) metabolites in bile of English sole (Parophrys vetulus) from Puget Sound, WA, USA by liquid chromatography/tandem mass spectrometry (LC-MS/MS)

Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous environmental contaminants that are widely monitored in marine biota from urbanized areas, due to their toxicity to aquatic organisms. Teleost fish can quickly metabolize PAHs into hydroxylated forms (OHPAHs) that, in some cases, are more toxic than the parent (unmetabolized) PAHs. But due to this fast metabolism, monitoring traditional parent PAHs in fish can cause underestimation on assessing PAH exposure. In addition, environmental levels of individual OHPAH metabolites are lacking in the literature worldwide. Therefore, we developed a rapid and accurate analytical method in which a number of individual OHPAHs metabolites are measured simultaneously in fish bile, via liquid chromatography coupled with tandem mass spectrometry, including low and high molecular weight mono- and diol-OHPAHs. We analyzed bile samples of 119 English sole (Parophrys vetulus) collected from 14 Puget Sound, WA, USA, sites, which has multiple sources of PAHs, including urban stormwater runoff, wastewater effluents, as well as an inactive creosote facility. The mean (± SD) biliary summed OHPAH (∑OHPAH) concentrations determined in English sole from urban, near-urban, and non-urban sites were 790 ± 1400 (n = 46), 310 ± 330 (n = 44) and 130 ± 200 (n = 29) ng/mL, respectively, with a maximum reaching 9400 ng/mL in a sample from an urban site. We compared these novel biliary OHPAH metabolite data with parent PAHs measured in stomach content of the same individual sole. Biliary ∑OHPAH concentrations were significantly correlated with the levels of ∑PAH in stomach content, however, with major differences in their distribution. We also demonstrated that biliary OHPAH metabolite data in English sole can potentially be used to distinguish different sampling sites due to a specific variety and intensity of PAH sources in the aquatic environment, which makes this a very important analytical approach for assessing PAH exposure in the environment.

Pollen limitation of native plant reproduction in an urban landscape.

Evidence suggests that bees may benefit from moderate levels of human development. However, the effects of human development on pollination and reproduction of bee-pollinated plants are less-well understood. Studies have measured natural variation in pollination and plant reproduction as a function of urbanization, but few have experimentally measured the magnitude of pollen limitation in urban vs. non-urban sites. Doing so is important to unambiguously link changes in pollination to plant reproduction. Previous work in the Southeastern United States found that urban sites supported twice the abundance of bees compared to non-urban sites. We tested the hypothesis that greater bee abundance in some of the same urban sites translates into reduced pollen limitation compared to non-urban sites. We manipulated pollination to three native, wild-growing, bee-pollinated plants: Gelsemium sempervirens, Oenothera fruticosa, and Campsis radicans. Using supplemental pollinations, we tested for pollen limitation of three components of female reproduction in paired urban and non-urban sites. We also measured pollen receipt as a proxy for pollinator visitation. We found that all three plant species were pollen-limited for some measures of female reproduction. However, opposite to our original hypothesis, two of the three species were more pollen-limited in urban relative to non-urban sites. We found that open-pollinated flowers in urban sites received less conspecific and more heterospecific pollen on average than those in non-urban sites. These results suggest that even when urban sites have more abundant pollinators, this may not alleviate pollen limitation of native plant reproduction in urban landscapes.

S3.5a Contamination in sand and water of sea, lakes, and river beaches: Risk factor for fungal human disease

S3.5 Environmental exposure - risk for human fungal disease, September 21, 2022, 4:45 PM - 6:15 PMMarine and freshwater bodies, such as rivers and lakes are known as possible niches harboring microbes that may affect human health.These ecosystems have been extensively researched, and even regulated, for the presence of bacterial contamination.Fungal contamination has been less researched and lacks regulation, notwithstanding the recent finding that the antifungal resistant yeast species Candida auris has been isolated from a marine ecosystem.The present lecture will focus on a recent collaborative study, involving 13 countries, spanning from the Atlantic to the Eastern Mediterranean coasts, and including the Italian lakes and the Adriatic, Baltic, and Black Seas. The study explored fungal contamination of sand and water of the sea, lakes, and river beaches in 91 bathing sites, and water of 67 of these.This study considered several fungal parameters: all fungi, several species of the genus Aspergillus and Candida, plus other yeasts, allergenic fungi, Dematiaceous fungi, and dermatophytes. The study took in account four variables that might influence the results of the analytical parameters, such as: (1) coast or inland location, (2) urban and non-urban sites, (3) period of the year, and (4) geographical proximity and type of sediment.A site-blind median was found to be 89 Colony-Forming Units (CFU) of fungi per gram of sand in coastal and inland freshwaters, with variability between 0 and 6400 CFU/g. For freshwater sites, that number was 201.7 CFU/g [0, 6400 CFU/g (P = .01) and for coastal sites was 76.7 CFU/g (0, 3497.5 CFU/g)]. For coastal waters and all waters, the median was 0 CFU/ml (0, 1592 CFU/ml), and for freshwaters 6.7 (0, 310.0) CFU/ml (P <.001). The fungal load in water has to be further corroborated in additional ongoing studies.Some of these will be presented herewith.The lecture will also include, as an example, the data collected at the Israeli Mediterranean coast. This study included a screen of sand and water of six urban beaches, from north to south of the Israeli Mediterranean Coast. Sand samples were extracted by water, and the water wash was cultured and quantitated. Water samples were quantitated as well. The fungi were identified phenotypically, by MALDI-TOF MS system and ITS sequencing. The screen revealed that about 80% of the isolates were molds and about 20% yeasts. The mold species included opportunistic pathogens and potential allergens: Aspergillus fumigatus, Fusarium, Penicillium, and Mucorales species. Yeast isolates included Candida, Cryptococcus and Rhodotorula species. The collaborative study and the example study confirmed the relevance of all variables for some of the fungal parameters. The results suggest that beaches should be monitored for fungi for safer use and better management, and for the benefit of public health.

Wild bee larval food composition in five European cities.

Urbanization poses threats and opportunities for the biodiversity of wild bees. At the same time, cities can harbor diverse wild bee assemblages, partly due to the unique plant assemblages that provide resources. While bee dietary preferences have been investigated in various studies, bee dietary studies have been conducted mostly in nonurban ecosystems and data based on plant visitation observations or palynological techniques. This data set describes the larval food preferences of four wild bee species (i.e., Chelostoma florisomne, Hylaeus communis, Osmia bicornis, and O. cornuta) common in urban areas in five different European cities (i.e., Antwerp, Belgium; Paris, France; Poznan, Poland; Tartu, Estonia; and Zurich, Switzerland). In addition, the data set describes the larval food preferences of individuals from three wild bee genera (i.e., Chelostoma sp., Hylaeus sp., and Osmia sp.) that could not be identified to the species level. These data were obtained from a Europe‐level study aimed at understanding the effects of urbanization on biodiversity across different cities and cityscapes and a Swiss project aimed at understanding the effects of urban ecosystems in wild bee feeding behavior. Wild bees were sampled using standardized trap nests at 80 sites (32 in Zurich and 12 in each of the remaining cities), selected following a double gradient of available habitat at local and landscape scales. Larval pollen was obtained from the bee nests and identified using DNA metabarcoding. The data provide the plant composition at the species or genus level preferred by each bee. These unique data can be used for a wide array of research questions, including urban ecology (e.g., diversity of food sources along urban gradients), bee ecology (characterization of bee feeding preferences), or comparative studies on the urban evolution of behavioral traits between urban and nonurban sites. In addition, the data can be used to inform urban planning and conservation strategies, particularly concerning flower resources (e.g., importance of exotic species and, thus, management activities). This data set can be freely used for noncommercial purposes, and this data paper should be cited if the data is used; we request that collaboration with the data set contact person to be considered if this data set represents an important part of the data analyzed in a study.

Spatial cover and carbon fluxes of urbanized Sonoran Desert biological soil crusts

Biological soil crusts (BSC) are important contributors to nutrient cycling in arid environments such as the Sonoran Desert. BSC at an urban (University Indian Ruins) and at a non-urban site (Santa Rita Experimental Range) were compared to determine if their structure or function was influenced by proximity to an urban environment. The Step Point method was used in the field to determine ground cover; which was found to be similar between sites. However, the spatial distribution of the BSCs was significantly different, such that more BSCs were found under plants at the non-urban site (P < 0.05). Relative gross photosynthesis was measured in the lab by addition of a watering event. Gross photosynthesis was found to be higher in the non-urban BSCs (P < 0.001), indicating lowered productivity in urban BSCs due to effects caused by proximity to urban environments. This study provides evidence that BSCs at urbanized sites are affected functionally, and therefore may be contributing differently to carbon and nitrogen cycling in these ecosystems.

A global urban microwave backscatter time series data set for 1993\u20132020 using ERS, QuikSCAT, and ASCAT data

Urban settlements are rapidly growing outward and upward, with consequences for resource use, greenhouse gas emissions, and ecosystem and public health, but rates of change are uneven around the world. Understanding trajectories and predicting consequences of global urban expansion requires quantifying rates of change with consistent, well-calibrated data. Microwave backscatter data provides important information on upward urban growth – essentially the vertical built-up area. We developed a multi-sensor, multi-decadal, gridded (0.05° lat/lon) data set of global urban microwave backscatter, 1993–2020. Comparison of backscatter from two C-band sensors (ERS and ASCAT) and one Ku-band sensor (QuikSCAT) are made at four invariant non-urban sites (~3500 km2) to evaluate instrument stability and multi-decadal pattern. For urban areas, there was a strong linear correlation (overall R2 = 0.69) between 2015 ASCAT urban backscatter and a continental-scale gridded product of building volume, across 8450 urban grid cells (0.05° × 0.05°) in Europe, China, and the USA. This urban backscatter data set provides a time series characterizing global urban change over the past three decades.

Wintering Town: Individual Quality of Migratory Warblers Using Green Urban Areas in a Neotropical City

Migratory birds can be familiar winter visitors of Neotropical cities. However, of the regional migrant species bird pool, only a few species are abundant in urban areas. Their presence inside cities has been positively related to green urban areas with high tree cover. However, urban elements like artificial lights can also attract them to cities. Habitat quality that enables energetic refueling for migrant birds is crucial in all their annual migratory stages. While some Nearctic cities offer a high-quality refueling habitat for migrant birds, we lack this information for Neotropical cities where migratory birds winter. In this study, we evaluate whether the urban green areas of a Neotropical city act as high-quality habitats for two abundant species of migratory warblers:Setophaga coronataandLeiothlypis ruficapilla.We assessed this by capturing birds inside three urban green areas and comparing their abundances, population structure, and individual quality (scaled-mass index—SMI) with individuals spending winter in natural vegetation habitats outside the city. We found that both species do not show differences in SMI between urban and non-urban sites. We also found thatSetophaga coronatahad a higher capture rate in urban than non-urban sites, whileLeiothlypis ruficapillahad similar capture rates in both habitats. Our findings indicate that the urban green areas of cities can act as suitable quality habitat during winter for two abundant migrant birds. Our results indicate that cities can have a higher conservation potential for a declining and sensitive group such as the Nearctic-Neotropical migratory birds than previously thought. We also point out the need to acknowledge that migratory species can be urban dwellers.

Environmental Determinates of Distribution for Dragonfly Nymphs (Odonata: Anisoptera) in Urban and Non-Urban East Texas Streams, USA

We collected environmental and habitat data for nymphs of 12 dragonfly species (Odonata: Anisoptera) from 91 stream sites throughout eastern Texas, including urban and non-urban locations. Understanding the relationship of dragonflies to habitat structure and other environmental variables is crucial for the purpose of conserving these insects and better using them as predictive tools for water quality assessments, and refining tolerance values. The objectives of this study were to determine the key environmental variables influencing the diversity and distribution of dragonflies in eastern Texas streams, and further determine if differences in those factors could be observed between urban and nonurban sites. We collected samples separately from benthic habitats and woody snag habitats. Significantly fewer sites were observed to have dragonfly species on snag habitat (mean = 1.25) compared to benthic samples (mean = 14.67) (t-test, p = 0.001). The number of dragonfly species collected among non-urban streams (mean = 9.83) was not significantly different than urban streams (mean = 6.08; t-test, p = 0.07). Detrended correspondence analysis of benthic and snag habitat data collected from non-urban and urban locations showed that most of the species are oriented most closely to benthic habitats in non-urban streams. Snag habitat was shown to be poorly ordinated for all of the species. A canonical correspondence analysis of 29 water quality and habitat variables as environmental determinants of dragonfly diversity and distribution showed that distributional relationships among species are complex and often described by multiple environmental factors.

Spatializing the roughness length of heterogeneous urban underlying surfaces to improve the WRF simulation-part 1: A review of morphological methods and model evaluation

Roughness length (Z0) is a critical parameter in atmospheric modeling that affects the land–atmosphere interactions. However, atmospheric models often use a fixed and relatively lower value for Z0 in urban areas, which cannot faithfully represent the actual heterogeneous urban underlying surfaces and reproduce the meteorological conditions. In this study, we provided a review of eight semiempirical models based on morphological methods from the literature and adopted them to develop a dataset of nonuniformly distributed roughness lengths in urban areas of Guangzhou, a highly urbanized city in China. In addition, we employed these datasets in the WRF model and evaluated the model. Overall, the estimated Z0 values in dense high-rise areas of the city were significantly higher than those in other regions. Different semiempirical models were found to generate significantly different Z0 values. The average local Z0 values of most models (7 out of 8) were higher than the model default value (0.5 m). Substituting the model default Z0 values with the localized non-uniform Z0 map revealed that the improvement in the simulated temperature and relative humidity at 2 m height was relatively small, whereas the improvement was significant for the simulated wind speed at 10 m height (WS10). The WRF simulations using the C71, T93, and K13 (see the main text for their definitions) schemes to estimate Z0 demonstrated the best improvements in the WS10 simulation of 54%, 50%, and 39%, respectively, compared with that using the default value. This improvement is of great value because many atmospheric models overpredict WS10. The simulation improved not only for the urban sites but also for the non-urban sites, demonstrating an improved ability for all stations. Among the schemes, the C71 and T93 semiempirical models were only applicable for low-density cities, whereas the K13 model applied to cities with significant urbanization. This study revealed the benefit of spatializing the heterogeneous urban roughness length to improve the meteorological simulations. This modification can be employed in the air quality model and further improve the simulations and forecasts of pollutant concentrations.

Genetic structure of urban and non-urban populations differs between two common parid species

Landscape conversions induced by human activities can affect dispersal patterns of various bird species and, as a result, affect genetic structure of their populations. Genetic differentiation of bird populations may be enhanced by habitat variation, especially in urban-non-urban systems. The majority of population genetic studies focus on single species, which inflicts limitations for direct comparisons of genetic responses of avian populations to urbanization. Here, we used a set of microsatellite markers to examine genetic diversity, gene flow and population structure in two common parid species, great tits Parus major and blue tits Cyanistes caeruleus occupying three sites in habitats with contrasting urbanization level in central Poland. We found low but significant divergence of urban park population with both suburban and non-urban forest great tit populations, while no differentiation was found between suburban forest and non-urban forest populations. In contrast, no evidence for genetic differentiation was found between blue tit populations from the urban park, suburban forest and non-urban forest sites. We conclude that great tits and blue tits respond to urbanization-related changes in a different way, which may be a result of different rates of migration and/or dispersal, likely higher in blue tits. Some impact may be also induced by interspecific competition. We suggest that changing the focus of urban genetic research from single to multiple species may provide novel insights into how natural populations respond to the processes of urbanization.

Spatiotemporal Variations in Fine Particulate Matter and the Impact of Air Quality Control in Zhengzhou

To study the spatiotemporal variations in fine particulate matter (PM2.5) and the impact of air quality management in autumn and winter in Zhengzhou, five sites were selected to collect PM2.5 samples from the autumn of 2017 to the winter of 2018, and the characteristics of the chemical components were analyzed. The positive matrix factorization (PMF) model was also applied to identify the sources of PM2.5, and the effect of air quality control was evaluated to provide support for air quality control in autumn and winter in the next stage. The PM2.5 concentrations in the four seasons in Zhengzhou were ranked as winter > autumn > spring > summer. The PM2.5 concentration at Zhengzhou University (ZZU) was the highest (8.7% higher than the average concentration), and the PM2.5 concentrations at the other sites were slightly lower than the average concentration. The concentration of water-soluble ions (WSIs) was low in spring and summer and high in autumn and winter. The average proportions of SO42-, NO3-, and NH4+ in the nine WSIs were as high as 22.5%, 43.6%, and 23.4%, respectively. The proportion of Cl- in winter was higher than that in the other seasons owing to coal combustion (6.7% and 6.6% in 2017 and 2018, respectively). Owing to wind and sand, the proportions of Ca2+ and Mg2+ in spring were the highest (4.4% and 0.4%, respectively), and those at the Jiancezhan (JCZ) and ZZU sites were higher than those at the other sites. K+, as a marker of biomass burning, had a higher proportion in spring, autumn, and winter. The proportion of K+ in the spring of 2018 was 1.9%, those in the autumn and winter of 2017 were 1.6% and 2.1%, respectively, and those in the autumn and winter of 2018 were 1.3% and 1.8%, respectively. JCZ, Hangkonggang (HKG), and Xinmi (XM) had higher proportions of NO3-, and the proportions of SO42- were lower. Secondary organic carbon (SOC) pollution was serious in autumn and winter, and the concentration accounted for more than half of the organic carbon (OC). In 2018, the SOC/OC at the JCZ and ZZU sites decreased compared with that in 2017, but that at the other three sites increased significantly, thereby indicating that different air pollutant emissions in these regions had different performances in response to control policies. The chemical composition reconstruction results showed that the proportion of sulfate was highest in summer (25.0%), the contribution of nitrate was higher in autumn (23.1% and 25.1% for 2017 and 2018, respectively) and winter (20.6% and 23.0% for 2017 and 2018, respectively), the proportion of crustal material was higher in spring (18.2%), and the contribution of secondary organic aerosol (SOA) was the highest in winter (14.1% and 20.5% for 2017 and 2018, respectively). SOA had higher contributions at the JCZ and HKG sites (16.9% and 16.4%, respectively), and ZZU was affected more by primary organic aerosol (14.3%) and crustal materials (12.1%). The PMF results showed that secondary inorganic salts (37.5%), SOA (15.4%), traffic (14.9%), industry (4.8%), coal combustion (16.0%), fugitive dust (6.5%), and biomass burning (2.8%) were the main pollution sources of PM2.5 in Zhengzhou. SOA and coal combustion contributed more in winter and fugitive dust contributed more in spring, followed by autumn. Biomass burning contributed more in spring and autumn. The urban sites JCZ and ZZU and the characteristic site HKG near the airport were more affected by traffic sources (16.9%, 16.2%, and 16.0%, respectively) than the other sites. The impact of biomass burning on the non-urban sites XM and HKG was slightly larger (both 2.7%), and the contribution of coal combustion to the suburban site XM was higher (16.8%). Owing to the construction around ZZU, the loading of fugitive dust at ZZU was higher than that at other sites. Comparing the results of the two-year autumn and winter, the contribution of SOA, traffic, and industry increased in the autumn and winter of 2018, whereas the contribution of secondary inorganic salts, coal combustion, and biomass burning decreased and the contribution of fugitive dust in winter also decreased. The results showed that the control strategies in autumn and winter had significant effects on the primary sources, including fugitive dust, coal combustion, and industry, and SOA precursor volatile organic compounds should be targeted for further pollution control.

Mycosands: Fungal diversity and abundance in beach sand and recreational waters — Relevance to human health

The goal of most studies published on sand contaminants is to gather and discuss knowledge to avoid faecal contamination of water by run-offs and tide-retractions. Other life forms in the sand, however, are seldom studied but always pointed out as relevant. The Mycosands initiative was created to generate data on fungi in beach sands and waters, of both coastal and freshwater inland bathing sites. A team of medical mycologists and water quality specialists explored the sand culturable mycobiota of 91 bathing sites, and water of 67 of these, spanning from the Atlantic to the Eastern Mediterranean coasts, including the Italian lakes and the Adriatic, Baltic, and Black Seas. Sydney (Australia) was also included in the study. Thirteen countries took part in the initiative. The present study considered several fungal parameters (all fungi, several species of the genus Aspergillus and Candida and the genera themselves, plus other yeasts, allergenic fungi, dematiaceous fungi and dermatophytes). The study considered four variables that the team expected would influence the results of the analytical parameters, such as coast or inland location, urban and non-urban sites, period of the year, geographical proximity and type of sediment. The genera most frequently found were Aspergillus spp., Candida spp., Fusarium spp. and Cryptococcus spp. both in sand and in water. A site-blind median was found to be 89 Colony-Forming Units (CFU) of fungi per gram of sand in coastal and inland freshwaters, with variability between 0 and 6400 CFU/g. For freshwater sites, that number was 201.7 CFU/g (0, 6400 CFU/g (p = 0.01)) and for coastal sites was 76.7 CFU/g (0, 3497.5 CFU/g). For coastal waters and all waters, the median was 0 CFU/ml (0, 1592 CFU/ml) and for freshwaters 6.7 (0, 310.0) CFU/ml (p < 0.001). The results advocate that beaches should be monitored for fungi for safer use and better management.

Spatio-temporal characterization of litter at a touristic sandy beach in South Brazil.

Litter is an ecological, economic, and social problem that impacts marine environments around the world. To create prevention and mitigation measures to solve this issue, it is necessary to understand the amounts and sources of this type of pollution. Cassino Beach is an extensive sandy beach located in South Brazil (∼220km in length) that presents multiple uses, such as touristic, portuary and fishery activities. In order to evaluate the spatial and seasonal variation of litter amounts, types and sources at Cassino Beach, litter (>2.5cm) was collected over 27monthsat two urban and two non-urban sites. At each site, the litter present in three 200m2 areas was sampled and evaluated. A total of 19,457 items were collected, mostly composed by plastic (∼88%). Paper, metal, and cloth items were also present, but in low amounts. Fragments and cigarette butts were the major types of plastic litter, with abundances of 28.4% and 17.0%, respectively. Urban sites presented higher amounts of litter, with those related to beach use being more common, emphasizing the contribution of beachgoers to litter input at these sites. During the summer season, when beach use increases, the highest total litter concentration was found. Undefined and/or beach use-related sources were dominant in all sites and seasons. Mapping the predominant materials, types and potential sources of litter creates important baseline data that can contribute not only to beach monitoring, but also to the development of litter reduction strategies.

Urban specialization reduces habitat connectivity by a highly mobile wading bird

BackgroundMobile animals transport nutrients and propagules across habitats, and are crucial for the functioning of food webs and for ecosystem services. Human activities such as urbanization can alter animal movement behavior, including site fidelity and resource use. Because many urban areas are adjacent to natural sites, mobile animals might connect natural and urban habitats. More generally, understanding animal movement patterns in urban areas can help predict how urban expansion will affect the roles of highly mobile animals in ecological processes.MethodsHere, we examined movements by a seasonally nomadic wading bird, the American white ibis (Eudocimus albus), in South Florida, USA. White ibis are colonial wading birds that forage on aquatic prey; in recent years, some ibis have shifted their behavior to forage in urban parks, where they are fed by people. We used a spatial network approach to investigate how individual movement patterns influence connectivity between urban and non-urban sites. We built a network of habitat connectivity using GPS tracking data from ibis during their non-breeding season and compared this network to simulated networks that assumed individuals moved indiscriminately with respect to habitat type.ResultsWe found that the observed network was less connected than the simulated networks, that urban-urban and natural-natural connections were strong, and that individuals using urban sites had the least-variable habitat use. Importantly, the few ibis that used both urban and natural habitats contributed the most to connectivity.ConclusionsHabitat specialization in urban-acclimated wildlife could reduce the exchange of propagules and nutrients between urban and natural areas, which has consequences both for beneficial effects of connectivity such as gene flow and for detrimental effects such as the spread of contaminants or pathogens.

Integrated Evaluation of Indoor Particulate Exposure: The VIEPI Project

Despite the progress made in recent years, reliable modeling of indoor air quality is still far from being obtained. This requires better chemical characterization of the pollutants and airflow physics included in forecasting tools, for which field observations conducted simultaneously indoors and outdoors are essential. The project “Integrated Evaluation of Indoor Particulate Exposure” (VIEPI) aimed at evaluating indoor air quality and exposure to particulate matter (PM) of humans in workplaces. VIEPI ran from February 2016 to December 2019 and included both numerical simulations and field campaigns carried out in universities and research environments located in urban and non-urban sites in the metropolitan area of Rome (Italy). VIEPI focused on the role played by micrometeorology and indoor airflow characteristics in determining indoor PM concentration. Short- and long-term study periods captured diurnal, weekly, and seasonal variability of airflow and PM concentration. Chemical characterization of PM10, including the determination of elements, ions, elemental carbon, organic carbon, and bioaerosol, was also carried out. Large differences in the composition of PM10 were detected between inside and outside as well as between different periods of the day and year. Indoor PM composition was related to the presence of people, to the season, and to the ventilation regime.

Urban forests near municipal solid waste incinerators do not show elevated trace metal or rare earth element concentrations across three cities in the northeast USA.

With the World Bank estimating global municipal solid waste MSW to increase to 3.4 billion tonnes by 2050, municipal solid waste incineration (MSWI) needs to be considered as part of sustainable waste management. In spite of major advancements in air pollution control systems and emission regulations, emissions of trace metals (TMs) (As, Cd, Cu, Ga, Mn, Ni, Pb, V, W, Zn) and rare earth elements (REEs) (La, Ce, Pr, Nd, Sm, Eu, Gd, Tb, Dy, Ho, Yb) may occur. Here, leaves, wood, forest floor, and A horizons in urban forests were assessed to determine if TM and REE concentrations show enrichment near MSWI relative to other urban areas for three cities in the northeastern USA. Vegetation and soil concentrations measured were generally comparable to concentrations observed in previous studies in non-urban sites. Overall, higher TM and REE in forest floor, A horizons, leaves, and wood were not observed consistently for MSWI sites when compared to city sites. Although pooled forest floor Ni and V and A horizon La and Nd concentrations were significantly different, they were not consistent but driven by a large difference at one of the three cities. This indirect study suggests air pollution control systems have prevented or have reduced TM and REE emissions to local urban forests to negligible amounts. Further studies are needed to evaluate potential interferences from other urban air pollution sources, and conduct direct emission measurements to validate MSWIs are not emitting TMs and REEs.

Patterns of fish use in urban estuaries: Engineering maintenance schedules to protect broader seascape habitat

Estuaries are experiencing high rates of modification because of urban development and on-going associated maintenance works which impacts their ecosystem functions and services. This research appraises the fish and crustacean community occupying urbanised estuaries, and whether necessary maintenance works (dredging, mangrove pruning) could be timed to better protect the ecosystem values. Using a range of sampling equipment we collected fish and crustacean assemblages from urban, semi-urban, and non-urban estuaries, in addition to water quality, tidal inundation, and marine plant densities to determine relationships among sites. Seasonal differences in body size distributions of socio-economically important species were also examined to infer timing of juvenile recruitment. Despite common assumptions that urbanised estuaries are degraded and provide little habitat value for coastal species, we found that the species composition of aquatic assemblages was similar between urban and non-urban sites, but differed according to tidal position (i.e. supralittoral vs. lower tidal). Seasonality in body size distributions differed among socio-economically important fisheries species, with some species showing juvenile recruitment during the wet season, dry season, or throughout the year. This study demonstrates that, regardless of urban vs. non-urban context, estuaries provide habitat for many aquatic animals, including socio-economically important fish species. Complexity associated with the dynamism of estuarine ecosystems and variability in constituent species’ life-history patterns makes management decisions regarding timing and delivery of urban waterway maintenance difficult. However, maintenance works that occur during Austral dry season (May to October) would provide the best protection for marine plants (non-flowering season for mangroves), while also reduces downstream movement of sediments and contaminants during wet season rainfall flow. Undertaking necessary maintenance works in the dry season provides grounds for wide scale seascape ecosystem optimism.