In this paper, a kernel-free quadratic surface support vector regression with non-negative constraints (NQSSVR) is proposed for the regression problem. The task of the NQSSVR is to find a quadratic function as a regression function. By utilizing the quadratic surface kernel-free technique, the model avoids the difficulty of choosing the kernel function and corresponding parameters, and has interpretability to a certain extent. In fact, data may have a priori information that the value of the response variable will increase as the explanatory variable grows in a non-negative interval. Moreover, in order to ensure that the regression function is monotonically increasing on the non-negative interval, the non-negative constraints with respect to the regression coefficients are introduced to construct the optimization problem of NQSSVR. And the regression function obtained by NQSSVR matches this a priori information, which has been proven in the theoretical analysis. In addition, the existence and uniqueness of the solution to the primal problem and dual problem of NQSSVR, and the relationship between them are addressed. Experimental results on two artificial datasets and seven benchmark datasets validate the feasibility and effectiveness of our approach. Finally, the effectiveness of our method is verified by real examples in air quality.