The probabilities of νμ→νe and ν‾μ→ν‾e oscillations in vacuum are determined by the CP-conserving flavor mixing factors Rij≡Re(UμiUejUμj⁎Uei⁎) and the universal Jarlskog invariant of CP violation Jν≡(−1)i+jIm(UμiUejUμj⁎Uei⁎) (for i,j=1,2,3 and i<j), where U is the 3×3 Pontecorvo-Maki-Nakagawa-Sakata neutrino mixing matrix. We show that Jν2=R12R13+R12R23+R13R23 holds as a natural consequence of the unitarity of U. This Pythagoras-like relation may provide a novel cross-check of the result of Jν that will be directly measured in the next-generation long-baseline neutrino oscillation experiments. Indirect non-unitarity effects and terrestrial matter effects on Jν and Rij are also discussed.