The morphological structure of retinal blood vessels plays an important role in diagnosing ophthalmic diseases such as glaucoma and diabetic retinopathy. Diabetic retinopathy is one of the leading causes of blindness worldwide. Because of the need to examine a large number of individuals on a daily basis, it is indeed challenging for ophthalmologists to analyze the complex retinal vasculature of every outpatient. Automated segmentation of retinal blood vessels can be of significant aid in this labor-intensive process. This work proposes an unsupervised approach for retinal blood vessel segmentation. Here, we enhance the performance of the B-COSFIRE filters for segmentation of the retinal vasculature by denoising a retinal image using a fractional filter derived from a left/forward weighted fractional integral prior to the vascular enhancement by B-COSFIRE filters. Also, we utilize hysteresis thresholding rather than a single global threshold to obtain the final segmented vessels enhanced by the B-COSFIRE filters. This helps to avoid the problem of nonuniform illumination and poor contrast in retinal images.