Four rectangular sluice gates were calibrated for submerged-flow conditions using nearly 16,000 field-measured data points on Canal B of the B-XII irrigation scheme in Lebrija, Spain. Water depth and gate opening values were measured using acoustic sensors at each of the gate structures, and the data were recorded on electronic data loggers. Several gate calibration equations were tested and it was found that the rectangular sluice gates can be used for accurate flow measurement. The Energy-Momentum (E-M) equations proved to be sound. The calibration of the contraction coefficient, to be used in the energy equation, allowed good estimations of the discharge for three of the four gates studied. The gate for which the E-M method did not perform satisfactorily was located at the head of the canal with a unique nonsymmetric approach flow condition. Alternatively, we investigated the performance of the conventional discharge equation. The variation of the discharge coefficient, Cd, with the head differential, Δh, and the vertical gate opening, w, suggests that Cd be expressed as a function of these two variables. For the sluice gates considered in this study, the best empirical fit was obtained by expressing Cd as a parabolic function of w, although an exponential expression tested previously by other writers also produced satisfactory results. The greatest uncertainty in the variables considered in this study was in the calculated coefficient of discharge, and based on the uncertainty analysis, it is possible to quantify the uncertainty in the estimated discharge through a calibrated sluice gate. The discharge uncertainty in each of the four gates in this study decreases with increasing gate opening, and it decreases slightly with increasing head differentials.