Abdominal radiographs are commonly used in dogs and cats that present with gastrointestinal signs. When initial abdominal radiographs are equivocal for the presence or absence of gastrointestinal mechanical obstruction, follow-up abdominal radiographs may be recommended. Based on our review of the literature, no published study has been performed to evaluate the clinical utility of serial abdominal radiographs in such cases. The purpose of this study is to determine whether follow-up abdominal radiographs increase diagnostic accuracy for mechanical obstruction. A prospective cohort study was performed on client-owned dogs and cats with clinical concern for gastrointestinal mechanical obstruction and initial abdominal radiographs inconclusive for the presence of obstruction. Follow-up abdominal radiographs were performed between 7 and 28h of the initial radiographs; an abdominal ultrasound performed within 3h of the follow-up study served as the gold standard. A total of 57 patients (40 dogs and 17 cats) were recruited; 19 of 57 cases (11 dogs; 8 cats) were mechanically obstructed, all with nonradiopaque foreign bodies. Four blinded reviewers (2 radiologists, 1 radiology resident, 1 criticalist) separately assessed the initial and the combined initial/follow-up radiographic studies for diagnosis of mechanical obstruction; for each observer, there was no significant change in accuracy (P=.058-.87) for the diagnosis of mechanical obstruction. Given the lack of significant increase in diagnostic accuracy using follow-up radiographs in cases of occult gastrointestinal mechanical obstruction, other diagnostic options (eg, abdominal ultrasonography) could be considered when survey abdominal radiographs are inconclusive for the diagnosis of mechanical obstruction in dogs and cats.