Nonprotein Respiratory Exchange Ratio Research Articles

Fat or Carbohydrate Oxidation during the Alpha Cardio Focus T25 Workout

The Shaun T Alpha Cardio Focus T25 workout is a 25 minute high-intensity aerobic workout. It is promoted as a calorie-burning, sweat-drenching workout designed to burn fat. However, the standard exercise prescription for metabolizing fat is moderate intensity for a prolonged period of time (>30 minutes). PURPOSE: The aim of this pilot study was to examine fuel oxidation and the lactate threshold in fit individuals during the alpha cardio Focus T25 workout. METHODS: Six active college-aged students performed the Focus T25 workout every other day for one week and data was collected on the third day. Subjects followed the Focus T25 DVD and performed moves like jumping jacks, pivot lunges, heel taps, lateral sprints, mountain climbers and control squats. Subjects moved for the full 25 minutes. Heart rate (HR) and non-protein Respiratory Exchange Ratio (npRQ) were measured every 30 seconds using a k4B2 mobile metabolic analyzer. Blood lactate was analyzed at 3 minute intervals via a finger prick and Lactate Plus analyzer while ratings of perceived exertion (RPE, 1–10 scale) were recorded every 5 minutes. RESULTS: Heart rate increased dramatically within the first few minutes of the workout and came close to reaching maximum levels via the standard maximum heart rate equation. Highest HR averaged 182 ± 14 bpm. The average npRQ during the workout was 0.95 ± 0.14, indicating primarily carbohydrate oxidation. Blood lactate levels averaged 5.2 ± 0.65 mmol/L just 4 minutes into the workout and peaked at 10 ± 1.44 mmol/L 12 minutes in. Subjects reported the highest RPE (6 ± 2) during the last ten minutes of the workout. CONCLUSION: This preliminary study indicates that individuals who perform the Alpha Cardio Focus T25 workout will 1) exercise at a high-intensity, 2) primarily burn carbohydrates, and 3) possibly feel the sensation of fatigue rather early into the workout.

Amino Acid-carbohydrate intake combined with multiple bouts of resistance exercise increases resting energy expenditure.

Increasing the rate of muscle protein synthesis is an energy consuming process that explains the acute elevations in resting energy expenditure (REE) observed 12 to 72 hours after a resistance exercise session. We hypothesized that multiple sessions of resistance exercise combined with the intake of amino acids would increase REE and alter the nonprotein respiratory exchange ratio (RER). Ten male participants completed two separate seven-day trials where REE and RER were measured on each morning via indirect calorimetry. On four consecutive days within each seven-day trial, acute resistance exercise was performed, and nutritional intake was manipulated by providing (1) amino acids and carbohydrate (AA-RT) or (2) nonnitrogenous, isoenergetic carbohydrate (CHO-RT) before and during each resistance exercise session. Average REE within the training period was 3.61% greater in AA-RT (7897 ± 252 kJ) compared to CHO-RT (7622 ± 289 kJ; P = 0.02). RER declined (P < 0.0001) from baseline after each resistance exercise was initiated in both AA-RT (0.82 ± 0.01 to 0.77 ± 0.01) and CHO-RT (0.82 ± 0.02 to 0.77 ± 0.02). We conclude the provision of amino acids with multiple bouts of resistance exercise enhances energy expenditure at rest without altering the utilization of lipid.

Effect of exercise on the diurnal variation in energy substrate use during a high-fat diet

Aerobic exercise increases 24-h fat oxidation following initiation of a high-fat diet. The objective of this study is to examine the time course of increased fat oxidation under exercise and sedentary conditions. Eighteen healthy subjects completed a randomized crossover design (sedentary and exercise visits) staying for five consecutive days in a metabolic chamber each visit. On day 1, 30% of energy intake was from fat; days 2-5 had 50% of energy as fat. During exercise, subjects rode on a stationary cycle at 45% of VO2max for 1 h in the mornings and evenings. Respiratory gases and urinary nitrogen were collected to calculate macronutrient oxidation and non-protein respiratory exchange ratio (NPRER). This data, collected continuously (24-h periods), were subsequently divided into three time segments: (1) exercise + recovery (1000-1200 hours, 2100-2200 hours), (2) sleep (2300-0645 hours), and (3) wake (all remaining hours). NPRER on exercise versus sedentary visits was lower for the sleep segment (0.77 ± 0.01 01 vs. 0.81 ± 0.01, p < 0.001), higher for the exercise + recovery segment (0.88 ± 0.01 vs. 0.86 ± 0.01, p < 0.001), and was not different for the wake segment. Fat oxidation was significantly higher for exercise versus sedentary treatments during sleep (41 ± 2 vs. 31 ± 2 g), wake (62 ± 3 vs. 51 ± 3 g), and exercise + recovery segments (33 ± 3 vs.16 ± 1 g), but so was fat intake by design (171 ± 8 vs. 128 ± 7 g/d). Although exercise showed greater fat oxidation during all segments, dietary fat intake was also higher. Therefore, based on NPRER, the time of day during which the exercise treatment increased the ratio of fat to carbohydrate oxidation was during sleep.

Timing Protein Intake Increases Energy Expenditure 24 h after Resistance Training

To determine whether protein supplementation (PRO) before an acute bout of heavy resistance training (HRT) would influence postexercise resting energy expenditure (REE) and the nonprotein respiratory exchange ratio (RER). REE would be increased and RER would be decreased up to 48 h after timed PRO and HRT compared with CHO supplementation and HRT. Eight resistance-trained subjects (five men and three women) participated in a double-blind two-trial crossover design, where REE and RER were measured (7:00 a.m.) on four consecutive days. On the second day of trial 1, subjects consumed 376 kJ of either PRO (18 g of whey protein, 2 g of carbohydrate, 1.5 g of fat) or CHO (1 g of whey protein, 19 g of carbohydrate, 1 g of fat) 20 min before a single bout of HRT (nine exercises, 4 sets, 70%-75% 1-repetition maximum). REE and RER were measured 24 and 48 h after HRT. During trial 2, the same protocol was followed except subjects consumed the second supplement before HRT. Compared with baseline, REE was elevated significantly in both CHO and PRO at 24 and 48 h after HRT (P < 0.05). At 24 h after HRT, REE in response to PRO was significantly greater compared with CHO (P < 0.05). RER decreased significantly in both CHO and PRO at 24 h after HRT compared with baseline (P < 0.05). No differences were observed in total energy intake, macronutrient intake, or HRT volume (P > 0.05). Timing PRO before HRT may be a simple and effective strategy to increase energy expenditure by elevating REE the day after HRT. Increasing REE could facilitate reductions in body fat mass and improve body composition if nutritional intake is stable.

Exercise increases the proportion of fat utilization during short-term consumption of a high-fat diet

Background: Increases in energy substrate oxidation occur at different rates after an increase in either fat or carbohydrate intake. Adaptations to increased fat intake are relatively slow and are influenced by activity level.Objective: We tested the hypothesis that increased levels of daily activity, as influenced by added exercise, would have a graded effect on the rate of compensatory adjustment to a short-term high-fat diet.Design: Daily total energy expenditure and macronutrient oxidation were measured at 3 physical activity levels (PALs) by using a whole-room indirect calorimeter in 10 adult women as they transitioned from a 1-d low-fat (30% of energy) control diet to a 4-d high-fat (50% of energy) diet. The 3 PALs (1.4, 1.6, and 1.8) were provided daily by increases in bicycle ergometer exercise time.Results: An increase in physical activity led to a greater increase in the nonprotein respiratory exchange ratio (−0.047 ± 0.02, −0.064 ± 0.02, and −0.071 ± 0.02; P < 0.0001) and 24-h fat oxidation (113 ± 24, 125 ± 19, and 147 ± 20 g/d; P < 0.0001) for PALs of 1.4, 1.6, and 1.8, respectively, after the transition from the low-fat control diet to the high-fat diet. Random-effects analysis found a significant (P = 0.003) relation between PAL and the compensatory fat oxidation response to a high-fat diet.Conclusions: Amounts of exercise consistent with the Institute of Medicine’s recommendations reduce the time required to match fat oxidation to a change in the percentage of fat in the diet. Because short-term consumption of high-fat diets is thought to contribute to excess fat accumulation, regular exercise should be protective and should help maintain a healthy body composition.

Gender differences in the regulation of amino acid metabolism.

Exercising men, compared with women, have a greater increase in leucine oxidation but not lysine rate of appearance. The cause for this sexual dimorphism is unknown; however, an inhibition of beta-adrenoreceptor activity has previously been shown to mediate amino acid metabolism (Lamont LS, McCullough AJ, and Kalhan SC. Am J Physiol Endocrinol Metab 268: E910-E916, 1995; Lamont LS, Patel DG, and Kalhan SC. J Appl Physiol 67: 221-225, 1989). This study was a gender comparison of leucine and lysine kinetics during a beta-adrenoreceptor blockade (beta1,beta2-blockade) and a placebo control by using a double-blind crossover protocol. Subjects exercised at 50% of their trial-specific maximal O2 consumption (1 h) after 7 days of dietary control. During exercise with beta-blockade, men had an increased nonprotein respiratory exchange ratio (P < 0.001), whereas women had an increased circulation of free fatty acids (P < 0.001). The genders also displayed distinct differences in exercise amino acid kinetics. The men, but not the women, increased leucine oxidation (P < 0.005) and lysine rate of appearance (P < 0.009) when exercising during beta-adrenergic blockade. This study indicates that during beta-blockade, exercising men increase their need for amino acids (and carbohydrate) to fuel energy needs, whereas women increase their mobilization of fat, thereby requiring less alternative fuels such as carbohydrate and amino acids. Gender-specific fuel preferences during exercise are regulated by beta-adrenergic-receptor activity. Substrate availability during exercise appears to modulate the amino acid oxidation differences between genders.

No effect of menstrual cycle phase on glucose kinetics and fuel oxidation during moderate-intensity exercise.

Resting and exercise fuel metabolism was assessed in three different phases of the menstrual cycle, characterized by different levels of estrogen relative to progesterone: early follicular (EF, low estrogen and progesterone), midfollicular (MF, elevated estrogen, low progesterone), and midluteal (ML, elevated estrogen and progesterone). It was hypothesized that exercise glucose utilization and whole body carbohydrate oxidation would decrease sequentially from the EF to the MF to the ML phase. Normal-weight healthy females, experiencing a regular menstrual cycle, were recruited. Subjects were moderately active but not highly trained. Testing occurred after 3 days of diet control and after an overnight fast (12-13 h). Resting (2 h) and exercise (50% maximal O(2) uptake, 90 min) measurements of whole body substrate oxidation, tracer-determined glucose flux, and substrate and hormone concentrations were made. No significant difference was observed in whole body fuel oxidation during exercise in the three phases (nonprotein respiratory exchange ratio: EF 0.84 +/- 0.01, MF 0.85 +/- 0.01, ML 0.85 +/- 0.01) or in rates of glucose appearance or disappearance. There were, however, significantly higher glucose (P < 0.05) and insulin (P < 0.001) concentrations during the first 45 min of exercise in the ML phase vs. EF and MF phases. In conclusion, whole body substrate oxidation and glucose utilization did not vary significantly across the menstrual cycle in moderately active women, either at rest or during 90 min of moderate-intensity exercise. During the ML phase, however, this similar pattern of substrate utilization was associated with greater glucose and insulin concentrations. Both estrogen and progesterone are elevated during the ML phase of the menstrual cycle, suggesting that one or both of these sex steroids may play a role in this response.

Substrate utilization and thermogenic responses to beta-adrenergic stimulation in obese subjects with NIDDM.

This study intended to investigate disturbances in beta-adrenergically-mediated substrate utilization and thermogenesis in obese subjects with mild non insulin-dependent diabetes mellitus (NIDDM). Following a baseline period of 30 min, the beta-agonist isoproterenol (ISO) was administered in increasing doses of 6, 12, and 24 ng/kgFFM x min, each dose for 30 min. Nine healthy lean males (CON, 50.6+/-2.4 y, % body fat: 16.0+/-1.8) and 10 obese subjects with NIDDM (51.8+/-2.4 y, % body fat 34.1+/-1.9). Basal non esterified fatty acid concentrations (NEFA) and basal fat oxidation (absolute or expressed per unit fat free mass, FFM) were significantly higher in NIDDM as compared to CON, whereas basal carbohydrate (CHO) oxidation was significantly lower. The ISO-induced increase in NEFA-concentrations was blunted in NIDDM (delta at 24 ng/kgFFM x min: CON: 717+/-59 micromol/l vs NIDDM: 358+/-97 micromol/l, P< 0.01). The non-protein respiratory exchange ratio (RER) did not change in NIDDM and significantly decreased in CON during ISO-infusion (P < 0.05), reflecting the tendency towards a blunted increase in fat oxidation in NIDDM (delta fat ox at 24 ng; CON: 0.025+/-0.005 g/min vs NIDDM 0.016+/-0.007 g/min). The ISO-induced thermogenic response was comparable in NIDDM and CON (at 24 ng %increase above baseline: CON: 16.8+/-2.2% vs NIDDM: 14.7+/-0.9%). At all time points, there were no significant differences in circulating ISO and noradrenaline concentrations. Basal adrenaline (A) concentrations and A concentrations during ISO-infusion were significantly lower in NIDDM (basal A; CON: 64+/-15 pg/ml vs NIDDM: 25+/-2 pg/ml, P < 0.001). There appear to be deviations in beta-adrenoceptor mediated fat utilization and adrenal medulla function in obesity-associated NIDDM. The impairments in sympathetically mediated fat utilization have previously been observed in 'simple' obese subjects, indicating that these disturbances are confined to the obese state per se.

D-Tagatose, a Stereoisomer of D-Fructose, Increases Hydrogen Production in Humans without Affecting 24-Hour Energy Expenditure or Respiratory Exchange Ratio

In growth studies on rats, the ketohexose D-tagatose has been shown to contribute no net metabolizable energy, and a pronounced thermic effect of the sugar has been suggested to account for the absence of energy. In a double-blind and balanced cross-over design, we measured 24-h energy expenditure in eight normal weight humans in a respiration chamber during the consumption of 30 g D-tagatose or 30 g sucrose/d. Metabolic measurements were performed before and after a 2-wk adaptation period with a 30-g daily intake of the test sugar. Total 24-h energy expenditure and hour-by-hour profile were unaffected by the test sugar. The nonprotein respiratory exchange ratio (RERnp) was similar during consumption of D-tagatose and sucrose. However, the effect on RERnp due to CO2 produced by fermentation of D-tagatose could not be quantified in this study. A significant increase in 24-h H2 production (35%) during D-tagatose administration suggests a substantial malabsorption of the sugar. We found no effects of the 2-wk adaptation period on the measured gas exchange variables. Significantly lower fasting plasma insulin and triglyceride concentrations were observed during D-tagatose administration compared with the sucrose period. No effects of D-tagatose on body weight and composition were seen, but the perception of fullness 2.5 h after the sugar load was greater with D-tagatose. In conclusion, this study does not suggest a pronounced thermic effect of D-tagatose, and other mechanisms seem to be required to explain its lack of net energy.

METABOLIC AND PERCEPTUAL RESPONSES TO SHORT-TERM CYCLE TRAINING IN CHILDREN962

This investigation examined the metabolic and perceptual response to short-term cycle training in children. Boys and girls were assigned to training (TG, N=10) and control (CG, N=13) groups. Following an orientation and practice session, maximal aerobic power (VO2max) was assessed on a calibrated cycle ergometer. On a separate occasion, subjects performed a submaximal test ride at 35, 45, 55, 65, and 75% of VO2max. Each intensity was performed for 6 min. with a 2-3 min. rest period between bouts. Substrate oxidation was estimated under steady-state conditions using the non-protein respiratory exchange ratio (RER) averaged over the last 3 min. of each bout. The rating of perceived exertion (RPE) was assessed at the end of each work bout. Following this initial evaluation, TG performed 12 cycle training sessions consisting of 3-10 min. work bouts, 3 times/week at 50% of VO2max. After 4 weeks, substrate oxidation and RPE were re-evaluated at the same absolute intensities in both groups. There were no differences (P> 0.05) between TG and CG with respect to age (9.8 ± 1.0 vs. 9.7± 1.2 yrs.), body mass index (21.7 ± 4.6 vs. 22.2 ± 9.5 kg/m2), percent fat (27.8 ± 8.4 vs. 27.1 ± 11.6%) and VO2max (37.9 ± 6.6 vs. 38.8 ± 7.8 ml kg-1 min-1). ANOVA on difference scores (post - pre test) for RER and RPE revealed a highly significant (P < 0.05) group effect for each dependent variable. Overall difference scores indicate a decrease in RER and RPE in TG, and an increase in RER and no change in RPE in CG. These data demonstrate that a short-term, cycle training program results in enhanced fat oxidation and increased exercise tolerance in young children as compared to a matched control group. These data may have relevance in our understanding of how to achieve a balance between fat intake and oxidation, a factor believed to be related to weight control.

Gender differences in substrate utilisation during exercise.

The selection and utilisation of metabolic substrates during endurance exercise are regulated by a complex array of effectors. These factors include, but are not limited to, endurance training and cardiorespiratory fitness, exercise intensity and duration, muscle morphology and histology, hormonal factors and diet. Although the effects of these factors on substrate utilisation patterns are well understood, the variation in substrate utilisation during endurance exercise between males and females is not. Because of the extreme heterogeneity in exercise protocols and individuals studied, the differences in substrate utilisation between males and females remain somewhat inconclusive. Regardless of heterogeneity, if the results from studies are interpreted collectively, an apparent gender difference in the selection and metabolism of substrates can be seen in sedentary individuals. However, this difference between genders diminishes as the level of cardiorespiratory fitness is increased to that of highly trained individuals. During rest and lower intensity exercise, the preferential metabolism of lipid occurs with a concomitant sparing of muscle glycogen. However, as the intensity of exercise is increased, the relative contribution of carbohydrate also increases. The exercise intensity at which the shift from lipid to carbohydrate is determined and regulated by the previously mentioned factors. Because the intensity and duration of exercise play a predominant role, the variation in exercise protocols poses a methodological concern when interpreting previous research. When attempting to compare the metabolism of substrates during endurance exercise, appropriate selection and interpretation of measurement techniques are necessary. Measurement techniques include the nonprotein respiratory exchange ratio, muscle and fat biopsies and the measurement of various blood metabolites, such as free fatty acids and glycerol. Similarly, in vitro analysis of lipolytic activity has also been demonstrated in males and females in response to varying levels of female gonadotrophic hormones. When comparing the substrate utilisation patterns between males and females, the area of hormonal regulation has received less attention. Often the catecholamine response to endurance exercise is measured; however, the gonadotrophic hormones, particularly those of the female, have received less attention when comparing genders. Indeed, the regulatory nature of the female gonadotrophic hormones has been demonstrated. Collectively, the effects of elevated estrogen, as in the luteal phase of menstruation, appear to promote lipolytic activity. Estrogen-mediated lipolytic activation occurs by apparently altering the sensitivity to lipoprotein lipase and by increasing the levels of human growth hormone (somatotropin), an activator of lipolysis.(ABSTRACT TRUNCATED AT 400 WORDS)

Oxidation rates of orally ingested carbohydrates during prolonged exercise in men.

Six male volunteers exercised on a cycle ergometer at 65% of maximal work load for 120 min on six occasions while ingesting water (W) only, four doses of maltodextrin (M) [0.92, 1.85, 2.77, and 3.70 g/kg body wt (4, 8, 12, and 16% M, respectively)], and sucrose (S) [1.85 g/kg body wt (8% S)]. Drinks were given during warm-up (8 ml/kg body wt) and each 15 min during exercise (2 ml/kg body wt). M and S were of high 13C natural abundance. Total carbohydrate (CHO) and fat oxidations were calculated from the nonprotein respiratory exchange ratio. M and S increased total CHO oxidation compared with W; no difference was observed between CHO solutions. Total CHO oxidation decreased continuously with time and more rapidly after W than after M or S. Fat oxidation increased continuously in all treatments. Oxidation rates of ingested CHO were 52 +/- 19, 76 +/- 12, 86 +/- 10, and 91 +/- 9 g/2 h for 4, 8, 12, and 16% M, respectively. The oxidation rate of S was 81 +/- 10 g/2 h (not different from 8% M), which indicated that the glucose polymer had no advantage over S. Oxidation rates of M and S increased to a plateau after 90-120 min of exercise. For all solutions except 4% M, the plateau oxidation rate was close to 1.0 g/min. Differences between 8, 12, and 16% M and 8% S were minimal such that ingestion of 8% M or S may well have had an optimal ergogenic effect.(ABSTRACT TRUNCATED AT 250 WORDS)

Short-Term Effects of Growth Hormone on Fuel Oxidation and Regional Substrate Metabolism in Normal Man

Primarily by increasing the availability of lipid intermediates, GH is likely to have profound effects on substrate consumption rates. To examine the short term actions of GH on glucose turnover, fuel oxidation and regional forearm metabolism, six normal volunteers were each studied twice for 5.5 h after having received a 4-h infusion of GH (20 ng/kg.min) or saline. GH induced slightly falling plasma glucose levels, acute 40-50% decreases in forearm glucose uptake, and no change in glucose turnover. Furthermore, substantial increases in circulating concentrations and forearm uptake of nonesterified fatty acids and 3-hydroxybutyrate were recorded. Although GH infusion was followed by a 50% reduction of forearm alanine release hepatic nitrogen excretion seemed unaffected. Energy expenditure was not influenced by GH, but the non-protein respiratory exchange ratio decreased from a basal value of 0.778 +/- 0.008 to 0.732 +/- 0.007 after GH treatment (P less than 0.05). Correspondingly, lipid oxidation increased from 1.20 +/- 0.06 to 1.48 +/- 0.09 mg/kg.min, and glucose oxidation decreased from 0.97 +/- 0.12 to 0.39 +/- 0.06 mg/kg.min (P less than 0.05). Nonoxidative glucose utilization tended to increase. These data indicate that GH, by promoting lipid utilization and decreasing glucose oxidation, diminishes the need for gluconeogenesis and, therefore, could be protein preserving in the long term. Overall, we found no evidence of GH having acute insulin-like effects on glucose metabolism. GH appears to increase glucose storage, leaving total energy expenditure unaffected.

Rates of energy substrates utilization during human cold exposure

Although it is well established in animals that acute cold exposure markedly increases the oxidation of energy substrates, the absolute quality and quantity of substrate oxidation is poorly understood in humans. This study compared the rates of substrate utilization in seven healthy young men exposed to both the warm (control exposure at 29 degrees C; semi-nude, 14 h fasted) and to the cold for 2 h (10 degrees C, 1 m.s-1 wind velocity). Substrate utilization was calculated using indirect calorimetry and the nonprotein respiratory exchange ratio, which was derived from the urinary urea nitrogen output. Cold exposure induced a 3.1 +/- 0.2 degrees C drop in mean body temperature and a body heat debt of 825.9 +/- 63.3 kJ (p less than 0.01). These parameters remained essentially unchanged in the warm. Cold exposure elevated the 2 h energy expenditure 2.46-fold in comparison to the warm (p less than 0.01). This cold-induced thermogenesis was accompanied by increases of 588% in carbohydrate oxidation (p less than 0.01) and 63% in fat oxidation (p less than 0.05), whereas protein oxidation remained unchanged. Although the greatest proportion of the energy expenditure in the warm was derived from lipid (59%), carbohydrate oxidation represented the major fuel for thermogenesis in the cold, since it accounted for 51% of the corresponding total energy expenditure. The results demonstrate that cold exposure causes a much greater increase in the utilization of carbohydrate than lipid. It is suggested that these substrates are directly utilized for thermogenesis in the shivering skeletal muscles.

Calorimetric validation of the Caltrac accelerometer during level walking.

The primary purpose of this study was to compare the Caltrac accelerometer output with measured energy expenditure (Ee). Twenty-five volunteers (10 men, 15 women) walked on a level motor-driven treadmill at four different speeds (54, 81, 104, and 130 m.min-1) with the Caltrac device affixed to the waistline. Each of the four experimental trials lasted eight minutes, and the testing was completed within an hour. During the test, oxygen consumption (VO2) (in L.min-1 and in mL.kg-1.min-1) and nonprotein respiratory exchange ratio were monitored by the Beckman Horizon metabolic cart. The accelerometer output at the end of each exercise bout was also monitored and subsequently divided by 8 to convert the readings to counts.min-1. The mean VO2 (L.min-1) at steady state (ie, 6th-8th minutes of exercise) was converted to a caloric value. We obtained a moderate correlation coefficient (r) of .76 between the accelerometer output and the VO2 (mL.kg-1.min-1) and a high correlation coefficient of .92 between the Ee and the accelerometer readings. The Caltrac accelerometer output (counts.min-1) was significantly higher (p less than .01) than the Ee (kcal.min-1) at the four walking speeds. The difference between the accelerometer output and the Ee ranged from 13.3% to 52.9%. The data were further analyzed with linear, polynomial, multiple, and stepwise regression models. The results of the analyses revealed that the Caltrac accelerometer output is a valid predictor of Ee during level walking when the appropriate regression equation is used to adjust the values.(ABSTRACT TRUNCATED AT 250 WORDS)