Microperforated polylactic acid (PLA) and polybutylene succinate (PBS) films were fabricated using a CO2 laser under various pulse fluences from 37.0 to 369.8 J/cm2, corresponding to pulse durations of 20–200 μs. A hole was formed at perforation thresholds of 147.9 J/cm2 for PLA and 129.4 J/cm2 for PBS films. The area and diameter of the microhole and volume losses increased with rising laser fluence. Microperforated PLA and PBS films showed similar mechanical properties of tensile strength, Young's modulus and elongation at break to films without microholes but significant improvements in oxygen transmission rate (OTR) and carbon dioxide transmission rate (CO2TR) over non-perforated films. OTR and CO2TR through a single microhole with area of ∼20,000 μm2 on PLA were 234 ± 58 and 217 ± 57 cm3/d, respectively while OTR and CO2TR though a single microhole on PBS were 205 ± 12 and 144 ± 40 cm3/d, respectively showing that biodegradable film could achieve very high OTR at up to 77,500 cm3/m2.d. Baby corn (100g) was packed in microperforated bags. Equilibrium modified atmosphere conditions of 10%–17%O2 and 4%–7% CO2 were reached in performed film packages with very high OTR and CO2TR of 35,000–77,500 and 36,500–80,000 cm3/m2.d, respectively. Results suggested that baby corn maintained freshness qualities for more than 7 days at a storage temperature of 15 °C. The CO2 laser perforation process was successful in producing modified atmosphere packaging (MAP) with tailored gas transmission rates.