Non-invaded Forests Research Articles

Invasive alien acacias rapidly stock carbon, but threaten biodiversity recovery in young second-growth forests.

Under the UN-Decade of Ecosystem Restoration and Bonn Challenge, second-growth forest is promoted as a global solution to climate change, degradation and associated losses of biodiversity and ecosystem services. Second growth is often invaded by alien tree species and understanding how this impacts carbon stock and biodiversity recovery is key for restoration planning. We assessed carbon stock and tree diversity recovery in second growth invaded by two Acacia species and non-invaded second growth, with associated edge effects, in the Brazilian Atlantic Forest. Carbon stock recovery in non-invaded forests was threefold lower than in invaded forests. Increasingly isolated, fragmented and deforested areas had low carbon stocks when non-invaded, whereas the opposite was true when invaded. Non-invaded forests recovered threefold to sixfold higher taxonomic, phylogenetic and functional diversity than invaded forest. Higher species turnover and lower nestedness in non-invaded than invaded forests underpinned higher abundance of threatened and endemic species in non-invaded forest. Non-invaded forests presented positive relationships between carbon and biodiversity, whereas in the invaded forests we did not detect any relationship, indicating that more carbon does not equal more biodiversity in landscapes with high vulnerability to invasive acacias. To deliver on combined climate change and biodiversity goals, restoration planning and management must consider biological invasion risk. This article is part of the theme issue 'Understanding forest landscape restoration: reinforcing scientific foundations for the UN Decade on Ecosystem Restoration'.

Ligustrum luciduminvasion decreases abundance and relative contribution of soil fauna to litter decomposition but increases decomposition rate in a subtropical montane forest of northwestern Argentina

Invasive plant species can alter litter decomposition rates through changes in litter quality, environment conditions, and decomposer organisms (microflora and soil fauna), but limited research has examined their direct impact on soil fauna. We assessed the abundance and relative contribution of soil meso- and macrofauna to litter decomposition in a forest invaded by Ligustrum lucidum W.T. Aiton and in a noninvaded forest in a subtropical mountain forest in northwestern Argentina, using litterbags (0.01, 2, and 6 mm mesh size). Additionally, we analyzed the litter quality and soil properties of both forest types. Soil fauna abundance was lower in the invaded forest than in the noninvaded forest. The contribution of soil macrofauna to litter decomposition was important in both forest types, but soil mesofauna contribution was significant only in noninvaded forest. Litter decomposition was significantly faster in the invaded forest, consistent with its higher quality litter compared with the uninvaded forest. Invaded forest had significantly lower litter accumulation, lower soil moisture, and greater soil pH than noninvaded forest. Our results showed that although soil fauna was less abundant and played a less pronounced role in litter decomposition in invaded forest, these changes did not translate into a reduced litter decomposition rate due to the higher quality of litter produced in the invaded forest.

How do invasive trees impact shrub layer diversity and productivity in temperate forests?

• Key messageInvasive tree species alter taxonomic diversity and functioning of forest shrub layers: Prunus serotina increases shrub layer biomass two to three times but decreases its biodiversity, Robinia pseudoacacia slightly increases shrub layer biomass and has no effect on its biodiversity, while Quercus rubra both biomass and biodiversity of the shrub layer.• ContextAlthough the impact of invasive trees on understory biodiversity is known, very little data exist about their influence on shrub layer biodiversity and productivity.• AimsTo assess impacts of Prunus serotina Ehrh., Quercus rubra L., and Robinia pseudoacacia L. on shrub layer aboveground biomass, species composition, and alpha diversity.• MethodsWe measured stand structures in a set of 168 study plots established in Wielkopolski National Park (W Poland), and we compared biomass and diversity metrics using generalized mixed-effects linear models.• ResultsWe found the lowest aboveground biomass of shrub layers in Q. rubra forests. P. sylvestris forests invaded by P. serotina had two to three times higher aboveground biomass than non-invaded forests. R. pseudoacacia forests had 27.8% higher shrub layer biomass than Quercus-Acer-Tilia forests. We found negative impacts of Q. rubra and negligible impacts of R. pseudoacacia on shrub layer alpha diversity metrics. However, the effect of Q. rubra was similar to native F. sylvatica. P. serotina negatively affected functional diversity, but its effects were lower in rich P. sylvestris forests than in poor P. sylvestris forests.• ConclusionThe introduction of alien tree species alters ecosystem services and species diversity of shrub layers. The direction and magnitude of these alterations are alien species-specific and context-dependent. Therefore, their management should account for their impacts.

Impacts of invasive trees on alpha and beta diversity of temperate forest understories

Despite good recognition of distributions and spread mechanisms of the three most invasive trees in Europe (Prunus serotina, Quercus rubra and Robinia pseudoacacia), their impacts on forest biodiversity are unevenly recognized. Most studies cover only taxonomic alpha diversity, and only a single study included functional and phylogenetic diversity. Using a set of 186 study plots in western Poland we assessed the impacts of these invasive tree species on the alpha and beta taxonomic, functional and phylogenetic diversity of understory vascular plants. Alpha diversity was higher in R. pseudoacacia forests and lower in Q. rubra forests compared to mature native forests. Compared to non-invaded plantations and forests, alpha diversity was higher in P. sylvestris plantations invaded by P. serotina, but lower in invaded nutrient-poor P. sylvestris forests. Alien species richness was higher and beta diversity was lower in forests invaded by P. serotina or R. pseudoacacia than in non-invaded forests. In contrast, beta diversity was higher in Q. rubra forests than in native forests. We proved that invaded forests differed from non-invaded forests in species composition, but not always with decreased alpha and beta diversity. Impacts of particular invasive species also depended on the reference ecosystem properties (here mature native forests, which did not always have the highest biodiversity), which is a source of inconsistency in previous studies, usually referring to single native ecosystem types.

Impact of Invasive Tree Species on Natural Regeneration Species Composition, Diversity, and Density

Invasive tree species decrease ecosystem resilience with negative impacts on natural regeneration. The influence of alien tree species on ecosystems is unevenly recognized and does not always account for different habitat specificity. We assessed the impacts of the three most frequent invasive tree species in European forests: Prunus serotina Ehrh., Quercus rubra L., and Robinia pseudoacacia L. on natural regeneration diversity, species composition, and density. We hypothesized that invaded forest types, in comparison with non-invaded, will differ in terms of species composition, will have lower taxonomic, functional, and phylogenetic diversity of natural regeneration, and will have lower densities of native tree species. We used a set of 189 study plots (200 m2) in a systematic design, established in various forest types in Wielkopolski National Park (West Poland). We analyzed impacts of forest type, accounting for soil C:N ratio, soil pH, and light availability on natural regeneration (woody species up to 0.5 m height) species composition, diversity, and density. We found an overlap of species composition among invaded and non-invaded forests and low impacts of invasive species on taxonomic diversity and functional richness. We found no impacts on phylogenetic diversity and other functional diversity components. In contrast, we found that the natural regeneration of forest-forming tree species reached lower densities in invaded than non-invaded forest types. However, sub-canopy and shrub species reached higher densities in invaded than non-invaded forest types. We confirmed that invasive tree species affect natural regeneration by decreasing the regeneration density of native tree species (in eight of nine tree species studied), species composition homogenization, and supporting natural regeneration of sub-canopy and shrub species. Therefore, the restoration of invaded forests requires eradication of invasive tree species to decrease propagule pressure and to stop decreases in the abundance of native tree species’ natural regeneration.

What happens to the mycorrhizal communities of native and exotic seedlings when Pseudotsuga menziesii invades Nothofagaceae forests in Patagonia, Argentina?

Pseudotsuga menziesii is one of the most widely planted conifers in the Patagonian Andes of Argentina, having invading characteristics that are broadly reported. We studied the mycorrhizal status of seedlings along six Nothofagaceae + P. menziesii invasion matrices to investigate their role in the invasive process, according to these hypothesis: a) The abundance and richness of EM will be higher in seedlings grown in their own soil; b) In the presence of native EM inoculum, the invasive plant will be associated with generalist mycorrhizae (EM and/or AM), c) AM associations will be more abundant in P. menziesii seedlings grown in Interface or native forest soils, d) Mycorrhizal community differences between treatments will alter host fitness (growth and nutritional parameters). Seedlings from Nothofagus dombeyi, N. antarctica, Lophozonia alpina, L. obliqua and Pseudotsuga menziesii were set up in a soil-bioassay that included soils from non-invaded Nothofagaceae forests, pure P. menziesii plantations, and the interface between both. Pseudotsuga menziesii seedlings showed a decreasing, although never null, ectomycorrhizal (EM) colonization pattern from plantations to non-invaded forests, mainly with exotic EM species. Hebeloma mesophaeum and Wilcoxina sp. 1, two EM species with cosmopolitan distribution, were found to be shared by both tree species. Hebeloma hiemale and Wilcoxina sp. 1, common mycorrhizal partners of P. menziesii in Patagonia although not registered from Nothofagaceae forest, were found to be associated with N. antarctica, being the first report for both fungal species. Pseudotsuga menziesii seedlings showed the ability to form different arbuscular mycorrhiza (AM) colonization types (Paris-, Arum-, Both- and Intermediate-types) depending on the treatments, with significantly higher presence of Intermediate-type in the Interface treatment, where colonization was low. The shared EM species and the presence of different AM colonization types imply enhanced possibilities for invasive P. menziesii seedlings establishment and development. Seedling features and EM colonization rates evidenced that P. menziesii invasion could produce maladaptation (defined as a relative decline in host fitness due to altered mycorrhizal communities from native settings) of mycorrhizal communities, seriously injuring native ecosystem.

Cricket calling communities as an indicator of the invasive ant Wasmannia auropunctata in an insular biodiversity hotspot

Invasive species are a major concern for the maintenance of ecosystem services and biodiversity but are difficult to mitigate. Upstream solutions to prevent their impact, including their detection, are needed. Wasmannia auropunctata, an invasive ant living in vagile supercolonies, is especially hard to track and is a major threat for tropical ecosystems and local animal communities. As part of such tropical communities, crickets are sensitive to ecological conditions, easy to collect, detectable and identifiable through their species-specific calls. Here, we evaluated the use of an acoustic community of crickets as an indicator of the presence of W. auropunctata in New Caledonia. We evaluated the dominance of the crickets in the soundscape, describe the cricket community structure and diversity along a shrubland to forest gradient, characterize these cricket communities structure and diversity in the light of ongoing invasion by W. auropunctata, and identify cricket species' indicators of the invasion. Acoustic recordings collected on 24 sites were described using human-listening and spectrographic visualization. The results demonstrated a clear dominance of the cricket group in the New Caledonian nocturnal soundscapes. Each habitat harbored a specific acoustic cricket community related to specific environmental attributes including vegetation height, daily variation of humidity and temperature. The presence of W. auropunctata was significantly associated with a lower cricket acoustic activity and species richness at night. Of the 19 species detected, four nocturnal species were identified as indicator of non-invaded forests and preforests. This work supports the use of acoustic as an alternative method to detect invasion.

Potential host and range expansion of an exotic insect-pathogen complex: Simulating effects of sassafras mortality from laurel wilt disease invasion in the central hardwoods region1

Abstract Laurel wilt disease is altering forests of the southeastern USA with widespread mortality to lauraceaous hosts, such as redbay (Persea borbonia (L.) Spreng.) and sassafras (Sassafras albidum (Nutt.) Nees). Sassafras is broadly distributed throughout the eastern USA and could serve as a pathway for laurel wilt disease spread into deciduous forests. The resulting invasion and widespread mortality of sassafras would affect these forests ecologically. In anticipation of this, we used preexisting vegetation data and the Forest Vegetation Simulator to predict future characteristics of invaded and noninvaded forests under two sassafras mortality scenarios, 33% and 99%. We calculated changes in total basal area and basal area of dominant species to assess the effects of invasion on forests of central Kentucky. We found that simulated sassafras mortality immediately resulted in significant declines to total basal area (0.87–2.32 m2/ha reductions) and also affected basal areas of selected species over time...

Do the invasive trees, Ailanthus altissima and Robinia pseudoacacia, alter litterfall dynamics and soil properties of riparian ecosystems in Central Spain?

Aims To assess the impacts of Ailanthus altissima and Robinia pseudoacacia on the whole litterfall process and on soil properties of riparian ecosystems of Central Spain by comparing invaded and non-invaded forests.

Unexpected earthworm effects on forest understory plants

BackgroundIntroduced earthworms are widespread in forests of North America creating significant negative impacts on forest understory communities. However, much of the reported evidence for negative earthworm effects comes from field investigations either comparing invaded and non-invaded forests or across invasion fronts. While important, such work is rarely able to capture the true effect of earthworms on individual plant species because most forests in North America simultaneously face multiple stressors which may confound earthworm impacts.We used a mesocosm experiment to isolate effects of the anecic introduced earthworm, Lumbricus terrestris L. on seedlings of 14 native plant species representing different life form groups (perennial herb, graminoid, and tree).ResultsEarthworm presence did not affect survival, fertility or biomass of any of the seedling plant species tested over a 17-week period. However, L. terrestris presence significantly decreased growth of two sedges (Carex retroflexa Muhl. ex Willd. and Carex radiata (Wahlenb.) Small) by decreasing the number of culms.ConclusionsOur mesocosm results with seedlings contrast with field reports indicating extensive and significant negative effects of introduced earthworms on many mature native forbs, and positive effects on sedges. We suggest that earthworm impacts are context- and age-specific and that generalizations about their impacts are potentially misleading without considering and manipulating other associated factors.

Understory Invasion by Acacia longifolia Alters the Water Balance and Carbon Gain of a Mediterranean Pine Forest

In water-limited ecosystems, where potential evapotranspiration exceeds precipitation, it is often assumed that plant invasions will not increase total ecosystem water use, because all available water is evaporated or transpired regardless of vegetation type. However, invasion by exotic species, with high water use rates, may potentially alter ecosystem water balance by reducing water available to native species, which may in turn impact carbon assimilation and productivity of co-occurring species. Here, we document the impact of invasion by an understory exotic woody species (Acacia longifolia) in a semi-arid Mediterranean dune pine forest. To quantify the effects of this understory leguminous tree on the water use and carbon fixation rates of Pinus pinaster we compare an invaded and a non-invaded stand. A. longifolia significantly altered forest structure by increasing plant density and leaf area index in the mid-stratum of the invaded forest. A. longifolia contributed significantly to transpiration in the invaded forest (up to 42%) resulting in a slight increase in stand transpiration in the invaded relative to non-invaded forest. More importantly, both water use and carbon assimilation rates of P. pinaster were significantly reduced in the invaded relative to non-invaded stand. Therefore, this study shows that exotic plant invasions can have significant impacts on hydrological and carbon cycling even in water-limited semi-arid ecosystems through a repartitioning of water resources between the native and the invasive species.

Removing an invasive shrub (Chinese privet) increases native bee diversity and abundance in riparian forests of the southeastern United States

Abstract. 1. Chinese privet (Ligustrum sinense Lour.) was removed from riparian forests in the Piedmont of Georgia in November 2005 by mulching with a track-mounted mulching machine or by chainsaw felling. The remaining privet in the herbaceous layer was killed with herbicide in December 2006. 2. Bee (Hymentoptera: Apoidea) abundance, diversity and community similarity in the forests were measured for 2 years after shrub removal and compared with heavily invaded controls and with non-invaded forests (desired future condition) using pan traps. 3. In 2006, control plots averaged 8.8 species and 34.8 bees per plot. Privet mulching resulted in 32.5 bee species and 418.3 bees per plot, and privet felling plots had 29 species and 259 bees per plot. In 2007, control plots averaged only10 species per plot and 32.8 bees per plot, while mulched and felled plots had 48 and 38 species per plot and 658.2 and 382.5 bees per plot, respectively. 4. The bee community on untreated control plots was dissimilar from the communities on privet felling, mulched and desired future condition plots during both years; however, by 2007, desired future condition, felling and mulched plots had similar bee communities. 5. Removal of an invasive shrub provided immediate benefits for native pollinators and resulted in bee communities similar to non-invaded forests even without further restoration of native plant communities.

Experimental analysis of the effect of exotic and native plant species on the structure and function of soil microbial communities

Invasions of exotic plant species are among the most pervasive and important threats to natural ecosystems, however, the effects of plant invasions on soil processes and the soil biota have rarely been investigated. We grew two exotic and a native under-story plant species in the same mineral soil from a non-invaded forest stand in order to test whether observed differences in the field could be experimentally produced in the greenhouse. We characterized changes in the soil microbial community structure (as indexed by PLFAs) and function (as indexed by enzyme activities and SIR), as well as changes in potential nitrogen mineralization rates. We found that the invasion of two very dissimilar exotic species into the under-story of deciduous forests in eastern North America can rapidly cause changes in most of the studied soil properties. At the end of the three-month incubation, soils under the exotic species had significantly different PLFA, enzyme and SIR profiles than both initial soils and soils where native shrubs had been grown. We also observed a significant increase in pH and nitrification rates under one of the exotic plants. Such changes in the soil are potentially long-term (e.g. changes in soil pH) and are therefore likely to promote the re-invasion of these and other exotics. Both management of exotic plant invasions and the restoration of native communities must take into account exotic species effects on the soil.