Nonhuman Bone Research Articles

A Review of Histological Techniques for Differentiating Human Bone from Animal Bone.

The first step in anthropological study is the positive identification of human remains, which can be a challenging undertaking when bones are broken. When bone pieces from different species are mixed together, it can be crucial to distinguish between them in forensic and archaeological contexts. For years, anthropology and archaeology have employed the histomorphological analysis of bones to evaluate species-specific variations. Based on variations in the dimensions and configuration of Haversian systems between the two groups, these techniques have been devised to distinguish between non-human and human bones. All of those techniques concentrate on a very particular kind of bone, zone, and segment. Histomorphometric techniques make the assumption that there are size, form, and quantity variations between non-humans and humans. The structural components of Haversian bones are significant enough to use discriminant function analysis to separate one from the other. This review proposes a comprehensive literature analysis of the various strategies or techniques available for distinguishing human from non-human bones to demonstrate that histomorphological analysis is the most effective method to be used in the case of inadequate or compromised samples.

The degradation of collagen in submerged bones, analysed by ImageJ® and Orbit®, for the estimation of the post-mortem interval (PMI) and the post-mortem submersion interval (PMSI)

ABSTRACT The estimation of the post-mortem submersion interval (PMSI) of skeletal remains can provide critical information to forensic investigations, contributing to various aspects such as identification. A fundamental aspect of the PMSI estimation in bone is the degradation of collagen, the most abundant organic component. This degradation can be assessed using various analytical techniques, including the digital analysis of images taken from histological slides. Two open-source software for image digital analysis, ImageJ® and Orbit®, provide novice-friendly and accessible technology to a broad audience of forensic practitioners. However, the absence of established procedures can challenge the consistency of the interpretation of the results, especially between observers. The principal aim of this study was to compare the efficacy of ImageJ® and Orbit® in quantifying the degradation of collagen in images taken from histological slides of mammalian non-human bones submerged for 4 months to 2 years. Despite the inconsistency in the outcomes of intraobserver vs. interobserver testing, this research has demonstrated the promising potential of digital histology. It has also identified the need for further investigation, particularly focusing on the usability of ImageJ® and the refinement of the Orbit® workflow.

Technical note: Temperature estimation accuracy based on colourimetry of embalmed human and fresh non-human burned bone

Estimation of the exposure temperature of skeletal remains can be done by means of colourimetry and a previously published decision model for the colourimetric data, resulting in clusters that represent a range of exposure temperature. The method was based on thermally altered freshly burned human skeletal remains. However, in practice the origin or pre-burning condition of (possibly fragmentary) burned remains can be unknown. Further, in order to use the colourimetric analysis, and accompanying decision model, it is important to have a reference or test set. Fresh human material is not available for this purpose in all countries. Hence, the classification accuracy of the decision model was tested for, in some countries more readily available substitutes for fresh bone; embalmed human bone and non-human bone. The model yielded high accuracies for these sample materials, making it possible to create a reference or test set from fresh non-human and embalmed human bone as substitute for human, and also to use the decision model for these deviating samples in practice.

Effects of water and other filters on fracture resolution in industrial micro-CT scanning

Filtering is known to improve image quality of CT scans. Water immersion is one type of filtering that has been used for CT scanning dry (skeletonized) bones, and it has been suggested that this approach can increase measurement accuracy and improve fracture resolution. These tests have previously involved clinical CT scanners. Here we use an industrial micro-CT scanner and test whether water immersion and other x-ray filtering options increases fracture resolution in reconstructed scans of dry bones.Eleven dry non-human bones were CT scanned using the same acquisition parameters, while varying filter options. Bones were scanned (1) in an unfiltered “dry” air environment, (2) using metal filters at the x-ray source, and (3) with the bones immersed in water. A small subset of bones (N = 3) was also scanned using the same parameters except increasing the number of projections acquired from 500 to 1500. Reconstructed scans were evaluated by the authors, in part using a Likert scale comparing filtered with unfiltered scans, to assess fracture resolution (overall appearance and extent).Results showed that increasing the projections resulted in the greatest improvement in fracture resolution, followed by filtering at the x-ray source. Water immersion performed poorly overall, possibly due to movement artifacts that result from this type of scanning, in which the specimen rotates on a stage during the scan. When using this type of CT scanner, if increased fracture resolution is desired, water immersion is not recommended; increasing the number of projections or filtering at the x-ray source is suggested instead.

A Study of Species and Individual Differentiation of Burnt and Unburnt Bones Using X-Ray Fluorescence

Commingled remains analysis is a fundamental problem in bioarchaeology and forensic anthropology. In cases of commingling, the bones of all individuals represented must be accurately and reliably differentiated. X-ray fluorescence spectrometry (XRF) can identify species and individuals when bone fragments are found in archaeological or forensic contexts. The present study aimed to verify whether portable X-ray fluorescence spectrometry (pXRF) can be used to differentiate bone fragments at the species level (Bos taurus, Sus domesticus, Equus caballus, and Homo sapiens) and at the individual level of human individuals. We also aimed to verify whether species and individuals can be differentiated using pXRF even if the bones were burned. A total of 119 adult human bones from archaeological sites in Central Moravia and Silesia and 17 adult non-human bones from archaeological sites in Central Moravia (Czech Republic) were examined. All bones are dated to various periods (from the Bronze Age to Modern Times). When differentiating the unburnt and burnt bones at the species level, the overall accuracy of classification was 84.6% and 93.9%, respectively. When differentiating unburnt human bones at the individual level, the correct classification ranged from 88.1% to 72.7%. The differentiation of the burnt bones of humans at the individual level achieved an average success rate of more than 60%. The results confirmed that pXRF can be used for species and individual differentiation of unburnt bones and is almost equally applicable to burnt bones.

Patterns of Violence in the Pre-Neolithic Nile Valley

Burial assemblages inform us about the biology of past societies, social relations, and ritual and symbolic behavior. However, they also allow us to examine the circumstances of death and social violence. A high level of intergroup violence among prehistoric hunter-gatherers is well-documented in some times and places but is extremely rare in others. Here we present an analysis of the perimortem injury to skeleton PD8 at the site of Sphinx in Central Sudan. This burial, attributed to the Early Khartoum (Khartoum Mesolithic) culture, radiocarbon dated between 8637 and 8463 cal BP, bears evidence of a perimortem sharp force trauma caused by penetration of an unshaped, fractured non-human bone between the right scapula and the rib cage. Among more than 200 anthropologically assessed human burials from the early Holocene Nile Valley reviewed in this paper, PD8 provides the only documented evidence of violence resulting in death. This rare case of death differs from the numerous cases of intergroup conflict documented in terminal Pleistocene burial grounds in Lower Nubia. This suggests different patterns of violence and strategies of conflict resolution in the pre-Neolithic (terminal Pleistocene and early Holocene) Nile Valley. We attribute this difference in the prevalence of interpersonal trauma to climatic and environmental conditions, territorial boundary defense, and post-marital residence practices before and after the Younger Dryas’ arid millennium (~ 12,800–11,600 BP).

Bone finds and their medicolegal examination: a study from Hesse, Germany

Bones found by chance can be of great criminal or historical interest. The nature of their appraisal depends on the individual case, the locally effective legislation and the available resources. To assess whether a find is relevant with respect to criminal investigation, the circumstances of the find and the results of the forensic examination carried out by trained personnel must be considered. The aim of this study was to obtain an overview of the circumstances and nature of the finds as well as the results of the subsequent expert opinions by evaluating bone finds from the federal state of Hesse, Germany. For this purpose, over a 10-year period from 2011 to 2020, all bone finds examined at the Institutes of Legal Medicine in Gießen and Frankfurt am Main, Germany, were evaluated retrospectively with regard to the locations and circumstances of the finds, their nature (human or non-human), the postmortem interval, possible traces of violent impact and the results of further examinations. Of the 288 bone finds evaluated, 38.2% were found in forests, meadows and parks. In 50.7%, the finds contained human bones, of which 37.0% had a forensically relevant postmortem interval of 50 years or less. Evidence of trauma was described in 77.4% of the human bone cases: postmortem damage in 78.8%, peri-mortem injury in 9.7% and ante-mortem injury in 11.5%. DNA examinations were performed in 40.4% of the human bone finds. They yielded STR profiles in 81.3%, leading to a definite identification in 35.4%. Among the non-human bones sent in, the most common were bones from pigs (23.4%), deer (18.1%), cattle (16.4%), roe deer (11.7%) and sheep (11.7%). The macroscopic examination is the first step of the forensic-osteological evaluation and sets the course for further examinations or investigations. DNA examinations are of great importance for the reliable identification of human bones. They were responsible for 70.8% of successful identifications.

Potential Issues in the Conservation of Bone and Teeth in Maritime Archaeology

Since the 1940s, when maritime archaeology was established, the systematic excavation of submerged wrecks began to be refined. Systematic excavations led to the recovery of a vast array of organic and inorganic artefacts, including human and non-human bones and teeth. In order to preserve the materials recovered from the sea, the discipline of maritime conservation rapidly expanded and dealt with unique issues, including, but not limited to, marine salts’ encrustation of archaeological artefacts. Bone and teeth are organic artefacts which provide crucial information about natural and social environments of the past. When recovered from marine settings, they are often friable and require conservation processes and consolidation treatments, e.g., to prevent shrinkage during drying. However, conservation processes and consolidants can interfere with pathological, traumatic and taphonomical evidence associated with bone and teeth, and can bias sample preparation and analysis through mechanical action and chemical composition. The aim of this paper, in which a few examples of interference are listed, is to highlight the need of accurately documenting any type of conservation process and/or consolidation treatment that has been performed on bone and teeth stored in archaeological collections. This becomes essential when samples are selected for study, especially if this includes destructive analysis, and will assist in clarifying any conflicting results, leading to reliable interpretations.

Human, All Too Human: Differentiating Non-Human from Human Bones in Protohistoric Cremation Contexts from Northern Italy

Differentiating cremated non-human bones from human ones in archaeological contexts is a challenging task. This analysis aims at proposing a rather solid criterion based on an osteoarchaeological sample. In this work, the main issues of taxonomic identification of cremated remains are analysed and a research methodology tested on an Italian protohistoric sample is proposed. The 314 subjects composing the sample come from 298 tombs of the Golasecca Civilization (1st millennium BC in north-eastern Italy). On a morphological basis, 246 bone fragments were selected from which as many thin sections were obtained for a histomorphological evaluation. From the analyses, we identified the presence of animals in burials, whereas a mere morphometric analysis was not able to recognize them. Furthermore, the taxonomic identification has allowed us to propose new hypotheses on the funerary rite of Golasecca linked to the zooarchaeological remains.

Integrating histology in the analysis of multispecies cremations: A case study from early medieval England

AbstractMethodological options for differentiating commingled human from nonhuman calcined remains are limited. A zooarchaeological analysis of human cremations from three early medieval sites in the Avon Valley (Warwickshire, England) identified commingled animal remains in burials from the site of Bidford‐on‐Avon, but not at the contemporary sites of Wasperton and Alveston Manor. A histological study was conducted to further investigate whether additional fragments of nonhuman bone could be identified and to quantify potential differences in preservation or cremation intensity between the sites. Bone fragments (n = 92) were selected from 44 cremation burials across the three sites for thin section preparation. Histological cross sections were observed to record the presence of fibrolamellar plexiform bone and secondary osteon banding, as well as to categorize the histological preservation and cremation intensity. The analysis did not identify any nonhuman remains from Wasperton or Alveston Manor, but nonhuman bone fragments were identified in the Bidford‐on‐Avon histology sample. These data supplement and support the findings of the macroscopic analysis that multispecies commingled cremations were only prevalent at Bidford‐on‐Avon. No statistically significant differences were identified in histological preservation or cremation intensity between the cemeteries. Variability in animal use in funerary rites between cemetery sites, rather than preservation bias, is therefore the likely explanation for the differential recovery of commingled nonhuman bone from excavated cremation burials. These results confirm that histomorphology is a useful tool to incorporate in the analysis of multispecies commingled cremations.

Distinguishing thermally altered bones from debris using imaging and fluorescence spectrometry

Bushfires and mass disasters from which result fatal fire victims are two types of events in which Forensic Anthropology acts aiding in the recovery of human remains and the identification of the victims. This is a challenging job since bones that have undergone fire-caused alterations can be unrecognizable. Therefore, collecting evidence at the scene is very important and should be thorough. To evaluate the potential of the application of optical techniques for the recovery and analysis of burnt skeletal material in forensic contexts, this exploratory study focused on analysing reflectance and luminescence properties of bone to differentiate between skeletal remains and debris. The sample includes burnt human bones, as well as non-human bones and debris (like metal, fabric, and others). The reflectance experiments revealed to be quite ineffective, not showing a response pattern that allowed for discrimination between skeletal remains and debris. Three techniques were used to detect luminescence, which included imaging (with a camera), fluorescence spectrometry, and laser-induced fluorescence spectroscopy (excitation: 440 nm). Luminescence results were more promising, registering a positive response for several samples, with a general consistency of results between the different methodologies. Nevertheless, burning conditions and individual characteristics (e.g., pathologies) can introduce limitations to the techniques.

Experimental Formation of Coffin Wear on Bone

Localized, eroded flat areas on bones recovered from former coffin burials are hypothesized to have been formed as “coffin wear,” caused by a combination of acidic groundwater pooling in a coffin that is slowly degrading over time, bones coming to rest against a flat surface, and overlying sediments applying pressure. Due to the time expected to be needed for this taphonomic effect to accrue, no experiments have tested this hypothesis. To shorten the timeframe, the authors exposed weighted and unweighted, already skeletonized pig (Sus scrofa) bones for a period of 20 two-week cycles and a sample of previously degraded, assorted nonhuman bones to acidic solutions for 12 two-week cycles. Flattened areas consistent with coffin wear were formed only on the previously degraded sample and were more prevalent and prominent on the subsample that had been weighted down during exposure. These findings may help clarify forensic investigations where bones are suspected to originate from a cemetery burial and not of a more recent origin or non-coffin burial. Additional testing under differing conditions is needed to explore this topic more fully, including long-term burial projects and examination of dated cemetery populations.

RADIOCARBON DATING ROZHOK I, A MIDDLE PALEOLITHIC OPEN-AIR SITE IN SOUTHERN RUSSIA

ABSTRACTSamples of bone and teeth from the Middle Paleolithic open-air site of Rozhok I, located on the Gulf of Taganrog, were dated by radiocarbon (14C). We obtained 26 accelerator mass spectrometry (AMS) dates on a large sample of nonhuman bone and teeth recovered by N. D. Praslov during 1961–1962 from 5 of the 6 occupation horizons at Rozhok I. Two rigorous pretreatment methods were applied to the samples: ultra-filtration and XAD resin. The 14C ages from the uppermost two layers (Horizons 1 and 2) are infinite with the exception of one age near the limit of the 14C method. By contrast, the dates from Horizon 3 and the two lowermost layers (Horizons 5 and 6) are all finite ages and some are less than 45,000 BP (uncalibrated). While there is evidence of some post-depositional disturbance, there is no evidence of a stratigraphic reversal of the cultural layers. The simplest explanation for the pattern is that bones and teeth in the lower layers have been contaminated by groundwater containing young carbon. We conclude that Rozhok I probably antedates 50,000 cal BP and is beyond the range of 14C dating.

Tooth hop variability in human and nonhuman bone: Effect on the estimation of saw blade TPI.

Forensic research has demonstrated that tooth hop (TH) is a valuable measurement from saw-cut bones as it can be used to estimate teeth-per-inch (TPI) of a saw used in postmortem dismemberment cases. However, error rates for TPI estimation are still under development and knowledge of how bone tissue affects TH measurements remains unclear. The purpose of this research was to investigate the effects of tissue variability through the use of different taxa on the accuracy and precision of TH measurements in the bone to estimate TPI of the blade. A total of 1766 TH measurements were analyzed from human, pig, and deer long bones cut by two 7 TPI saw blades of different tooth type. Fifty distance-between-teeth measurements before and after sawing were collected directly from each blade for comparison to bone-measured TH to assess potential effects of tooth wear on TH variability. ANOVA and F tests were used to compare mean TH and variance, respectively, by saw-species (i.e., crosscut-deer, rip-deer) and species groups (i.e., all deer, all pig), with significance determined at the p<0.05 level. TH measurements were converted to usable TPI ranges, which would typically be presented in a forensic report. It is concluded that significant differences in TH (mm) do not necessarily reflect significant differences in associated TPI ranges of suspect blades. Forensic reports should report mean TPI±1.5-2.5 TPI while providing a sample size indicating number of TH measured rather than just number of cuts or cut surfaces examined.

The Floating Island Cave mammals: Paleoecology, abundance indices, and human subsistence through a taphonomic lens

Analyses of mammal remains from middle and late Holocene deposits in Floating Island Cave on the arid floor of the Bonneville basin in western North America are presented. Identified specimens consist of various desert mammals, especially leporids, which mirror collections from neighboring sites and provide additional data for one of the most comprehensive Holocene zoogeographic histories in the world. Taphonomic analyses identified large- and especially small-bodied mammal bones that were deposited by both human and non-human predators. Most horizons contained unusually high proportions of burned bones which are often viewed as unambiguous indicators of human subsistence activities. However, analyses of the cave’s deposits and the numbers and extent of burned bone indicate many specimens were charred in situ by post-depositional burning of the dry vegetal-rich fill. We employed abundance index measurements comparing artiodactyls against smaller leporids to investigate potential changes in human subsistence. We calculated two indices to illustrate the effects of non-human bone accumulations on abundance measures; one encompasses only specimens identified to genera and the other discounts scatological bones and other non-human accumulations and includes only bones and bone fragments classified as cultural refuse. When discounting the hundreds of leporid bones deposited by non-human vectors, the analyses show that the remains of leporids (especially hares [Lepus sp.]) are appreciably more abundant than artiodactyls, even during late Holocene periods of increased moisture. Given the dominance of Lepus in regional archaeological collections we argue that hares were not a low-ranked prey item but a low-risk dietary staple pursued throughout Holocene human occupation.

Geographically structured genomic diversity of non-human primate-infecting Treponema pallidum subsp. pertenue.

Many non-human primate species in sub-Saharan Africa are infected with Treponema pallidum subsp. pertenue , the bacterium causing yaws in humans. In humans, yaws is often characterized by lesions of the extremities and face, while T. pallidum subsp. pallidum causes venereal syphilis and is typically characterized by primary lesions on the genital, anal or oral mucosae. It remains unclear whether other T. pallidum subspecies found in humans also occur in non-human primates and how the genomic diversity of non-human primate T. pallidum subsp. pertenue lineages is distributed across hosts and space. We observed orofacial and genital lesions in sooty mangabeys (Cercocebus atys) in Taï National Park, Côte d’Ivoire and collected swabs and biopsies from symptomatic animals. We also collected non-human primate bones from 8 species in Taï National Park and 16 species from 11 other sites across sub-Saharan Africa. Samples were screened for T. pallidum DNA using polymerase chain reactions (PCRs) and we used in-solution hybridization capture to sequence T. pallidum genomes. We generated three nearly complete T. pallidum genomes from biopsies and swabs and detected treponemal DNA in bones of six non-human primate species in five countries, allowing us to reconstruct three partial genomes. Phylogenomic analyses revealed that both orofacial and genital lesions in sooty mangabeys from Taï National Park were caused by T. pallidum subsp. pertenue . We showed that T. pallidum subsp. pertenue has infected non-human primates in Taï National Park for at least 28 years and has been present in two non-human primate species that had not been described as T. pallidum subsp. pertenue hosts in this ecosystem, western chimpanzees (Pan troglodytes verus) and western red colobus (Piliocolobus badius), complementing clinical evidence that started accumulating in Taï National Park in 2014. More broadly, simian T. pallidum subsp. pertenue strains did not form monophyletic clades based on host species or the symptoms caused, but rather clustered based on geography. Geographical clustering of T. pallidum subsp. pertenue genomes might be compatible with cross-species transmission of T. pallidum subsp. pertenue within ecosystems or environmental exposure, leading to the acquisition of closely related strains. Finally, we found no evidence for mutations that confer antimicrobial resistance.

Using histomorphometry for human and nonhuman distinction: A test of four methods on fresh and archaeological fragmented bones

Positive identification of human remains is the very first step in anthropological analysis, and the task may be particularly difficult in the case of fragmented bones. Histomorphometry methods have been developed to discriminate human from nonhuman bones, based on differences in the size and shape of Haversian systems between the two groups. Those methods all focus on a very specific type of bone, section, and zone. Therefore, the objective of this study was to test the efficiency of four histomorphometric methods on a sample of fragmented bones. The sample is composed of 37 archaeological and fresh specimens, 25 nonhumans (Bos taurus, Equus caballus, Sus scrofa, Capreolus, Canis familiaris, Cervus elaphus, Ovis, and Capra) and 12 humans (Homo sapiens). Eight histomorphometric criteria were collected from all intact osteons visible on each fragment and then inserted into the corresponding discriminate function of each method. The results were compared with the real origin to establish rates of correct classification for each method. The methods of Martiniaková et al. (2006) and Crescimanno and Stout (2012) obtained very low percentages of good classification (32 % and 67 %). Those of Cattaneo et al. (1999) obtained 94 % correct classification, but only after a correction of the units of measurement for Haversian canal area in their formula. The methods of Dominguez and Crowder (2012) obtained an 86 % rate for well-classified specimens. Some of the methods tested here contain errors in the original publication that make them unusable in their current state. Plus, it seems that histomorphometric methods developed from specific areas are more difficult to apply to fragments. A reduced number of intact osteons analyzed may partially affect the reliability of the method by being unrepresentative of the entire microstructure. Therefore, this study demonstrates that one should be cautious with the use of histomorphometric methods to distinguish human and nonhuman fragmented bone until further research can refine these methods to achieve greater reliability.

Non-human bones in forensic casework: not such a trivial problem.

When bones are submitted to mortuaries significant time and resources from both police and anthropologists are required, even if they are subsequently determined to be non-human. A survey of non-human bone casework was made in order to determine the scope of this problem and the different bone types being assessed. This study used data from nine years of casework at the NSW Department of Forensic Medicine (DOFM), Sydney. It analyzed the number of non-human cases that were assessed, the types of animals represented in these bones, the bone elements found, their condition and their location when found. By 2016, over 70% of skeletal cases handled each year by the Sydney DOFM were non-human. The most common animal remains were sheep and cattle, and the skeletal elements appearing in the greatest number of cases were vertebrae, followed by femora and tibiae. Skull fragments were rare. Slightly more cases were found on the surface rather than buried. Fragmentation might have been expected to contribute to difficulties in identifying bones, but in fact 32% of cases consisted of complete bones only. This study shows a very high proportion of non-human forensic casework in the Sydney region. Data presented in this study may prove useful in designing training workshops in non-human bone identification.

Using nutrient foramina to differentiate human from non-human long bone fragments in bioarchaeology and forensic anthropology

Long bone shaft fragments can be found isolated in archaeological and forensic contexts, such as in mass fatality incidents. When diagnostic morphological landmarks are not visible, the assessment of a human or non-human origin of a bone fragment can be challenging. Further methods need to be developed. In long bones, the presence of a nutrient foramen on fragments that do not show any diagnostic landmarks can make the assessment of the origin of the bone still possible. In this paper, human long bones were compared to those of the following species: chicken (Gallus gallus domesticus), duck (Cairina moschata), sheep (Ovis aries), pig (Sus scrofa domesticus), and deer (fallow deer, Dama dama, and roe deer, Capreolus capreolus). Macroscopic evaluation of location, direction and appearance, and micro-CT scanning in order to measure angle and shape of canal entrance, were applied in this study to differentiate human from non-human nutrient foramina on long bone shafts. Observations regarding the location and direction of nutrient foramina were proven to be different between human and non-human bones; however, these two features might not be exploitable in cases of highly fragmented bones. The foramina appearance was the most reliable for the origin identification, although the shape of the canal entrance and its angle at the cortical bone, obtained from micro-CT scans, were also useful parameters. For a correct identification of a fragment, one parameter may not be enough and it is advisable to employ as many features as possible. This research demonstrates that nutrient foramina have potential as a reliable bone feature for the distinction between human and non-human fragmented and incomplete long bones.

Optimizing parameters for the use of alternate light sources in detecting fragmentary bones: a pilot study

ABSTRACTRecent studies have suggested that alternate light sources (ALS) are effective in detecting bone and teeth fragments at crime scenes. These studies, however, are few in number and provide no consensus as to the optimum combination of wavelength and filter for best detecting bone from non-bone material. Additionally, each study has been conducted using different environments, bone species and varying degrees of exposure to taphonomic factors. The aim of this study was to investigate the optimum parameters of ALS for the detection of bone and teeth fragments in a laboratory setting. Human and non-human bone, and tooth fragments were compared with non-bone material using handheld Rofin Poliflares. Temperature-treated bones (frozen, boiled and burnt) were included to assess if taphonomic factors affected fluorescence. Wavelengths ranging from 415 nm–530 nm were used and fluorescence was recorded using photographic images, and intensity analysed in ImageJ. Results of this pilot study found that the 450 nm Poliflare with orange filter provided the best discrimination of bone/tooth versus non-bone when used at a distance of up to 100 cm from the objects. Fluorescence of heat-treated bones is weakened at distances greater than 30 cm, which should be taken into account when temperature modification to remains is suspected.