This study aims to evaluate the possible occurrence during seismic shaking of interaction effects between the shallow subsoil and the overlying buildings of the Villa d’Agri town (Southern Italy). From the geological and building heritage points of view, the investigated area represents a typical town along the Apennines chain and therefore the obtained results could be easily exported to similar urban areas. A total of 239 single-station seismic ambient noise measurements were thus performed, 154 on the urban soils and 85 within buildings. Both subsets of measurements were planned to cover the main outcropping lithologies and building typologies. By means of the Horizontal-to-Vertical Noise Spectral Ratio technique, isofrequency and isoamplitude maps have been reconstructed for the whole area. The results evaluated on the 65 tested buildings allowed to infer the empirical relationship between the first vibrational period, T(s), and their height, H(m). Using this relationship and available information on the height of each building, the first vibrational frequency was estimated for 659 buildings belonging to the urban area of Villa d'Agri. Finally, in order to assess the possible occurrence of the double resonance phenomenon, we compared the main frequency of each building with that interpolated for the underlying foundation soil. The results show that the probability of being affected by the double resonance effect is very high only for a couple of buildings, high for about 16% of the buildings, moderate for the 23% and practically null for most buildings (60%).