An effective and universal positioning algorithm is essential for a highly reliable and accurate foot-mounted inertial pedestrian navigation system. Algorithm performance is significantly influenced by the accuracy of noise level estimation. This study introduces a novel blind noise estimation algorithm to reduce the impact of uncertain and variable system noise characteristics on positioning accuracy. The algorithm combines a quasi-population growth wavelet transform threshold with an adaptive window length adjustment strategy. Firstly, a quasi-population growth wavelet threshold function is employed to separate motion signals from noise and assess noise levels. Secondly, an adaptive sliding window strategy based on motion-noise correlation optimizing the balance between estimation precision and computational efficiency. These methods enable robust noise level estimation without prior knowledge and achieve real-time adjustments to the system noise matrix in Extended Kalman Filter of the inertial navigation system, thereby significantly enhancing navigation error suppression. The effectiveness of above methodologies has been experimentally validated.