Bimetallic Co-N/Zn@NPC (nanoporous carbon) derived from bimetallic zeolitic imidazolate frameworks (BMZIFs) has been synthesized using the co-precipitation method followed by carbonization 800 °C for 4 h in a nitrogen atmosphere. The synthesized Co-N/Zn@NPC was characterised by numerous physiochemical techniques such as Fourier-Transform infrared (FTIR), Raman spectroscopy, Scanning Electron Microscope (SEM), Tunnelling Electron Microscope (TEM), Selected Area Electron Diffraction (SAED) and X-ray Photoelectron Spectroscopy (XPS). The as-synthesized nanocomposite was utilized as an electrocatalyst to modify the glassy carbon electrode (GCE) for the electrochemical evaluation of uric acid (UA) and ascorbic acid (AA) in individual and dual modes in phosphate buffer solution (PB) in pH-6. The electrode kinetic behaviour of the Co-N/Zn@NPC has been studied by electrochemical impedance spectroscopy (EIS). The prepared electrode material demonstrates high electroactive surface area and appraisable electron transfer ability. It expresses an exquisite linear range of 0.1 to 14.7 μM for uric acid (UA) and 0.1 to 157.1 μM for ascorbic acid (AA), respectively. The present Co-N/Zn@NPC system has an excellent detection of limit (LOD) with 0.21 nM for UA and 7.65 nM for AA. The practical feasibility analysis shows a recovery of 99.62% and 99.00% for UA and AA in human blood serum samples.