Night-heron Eggs Research Articles

Legacy persistent organic pollutants (POPs) in eggs of night herons and poultries from the upper Yangtze Basin, Southwest China.

Black-crowned night heron (Nycticorax nycticorax) eggs have been identified as useful indicators for biomonitoring the environmental pollution in China. In this study, we investigated thirty eggs of black-crowned night heron collected from the upper Yangtze River (Changjiang) Basin, Southwest China, for the occurrence of legacy persistent organic pollutants (POPs), including polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs), organochlorine pesticides (OCPs), polychlorinated biphenyls (PCBs), and polybrominated diphenyl ethers (PBDEs). Our results showed a general presence of POPs in night heron eggs with OCPs being the dominant contaminants, having a geometric mean concentration of 22.2 ng g-1 wet weight (ww), followed by PCBs (1.36 ng g-1 ww), PBDEs (0.215 ng g-1 ww), and PCDD/Fs (23.0 pg g-1 ww). The concentration levels were found to be significantly higher in night heron eggs than in poultry eggs by one or two magnitude orders. Among OCP congeners, p,p'-DDE was found to be predominant in night heron eggs, with a geometric mean concentration of 15.1 ng g-1 ww. Furthermore, species-specific congener patterns in eggs suggested similar or different sources for different POPs, possibly associated with contaminated soil and parental dietary sources. Additionally, estimated daily intakes (EDIs) were used to evaluate non-carcinogenic and carcinogenic risk associated with consumption of bird eggs. Our results revealed non-negligible non-cancer and cancer risk for humans who consume wild bird eggs as a regular diet instead of poultry eggs.

Organophosphate flame retardants in heron eggs from upper Yangtze River basin, southwest China

The egg samples of four heron species, including black-crowned night heron (Nycticorax nycticorax), little egret (Egretta garzetta), Chinese pond heron (Ardeola bacchus) and cattle egret (Bubulcus ibis), were collected from the upper Yangtze River (Changjiang) Basin, Southwest China in early summer of 2017. Nine out of ten target organophosphate flame retardants (PFRs) were detected in these heron egg samples. The sum of concentrations of the PFRs quantified (∑PFRs) ranged from 63 to 590 pmol g−1 ww (18–185 ng g−1 ww) with a median value of 139 pmol g−1 ww (48 ng g−1 ww) among all samples. The median ∑PFRs in eggs of night herons (160 pmol g−1 ww) was higher than Chinese pond herons (median 121 pmol g−1 ww) and little egrets (median 109 pmol g−1 ww). In heron eggs, ∑PFRs were mainly contributed by tri-n-butyl phosphate (TNBP), tris (isobutyl) phosphate (TIBP), tris (1-chloro-2-propyl) phosphate (TCIPP) and tri-2-methylphenyl phosphate (TMPP). Alkyl-PFRs accounted for approximately 28%–85% (median 57%) of the nine PFRs quantified while the rest is contributed by aryl-PFRs and chlorinated PFRs. Lower levels of PFRs in little egret eggs were found upstream than downstream of the Yangtze. In addition, the daily intakes of PFRs through ingestion of heron eggs were estimated at lower levels.

Metal Levels in Eggs of Waterbirds in the New York Harbor (USA): Trophic Relationships and Possible Risk to Human Consumers

Health professionals are interested in evaluating the risks that heavy metals pose to eco-receptors and humans. The objective of this study was to examine levels of mercury (Hg), lead (Pb), cadmium (Cd), and other contaminants in waterbirds nesting in the New York harbor in 2012 to determine (1) whether there were species and locational differences, and (2) whether consumption of eggs posed a health risk to predators or humans. For arsenic (As), Pb, Hg, and selenium (Se), species contributed more to variations in levels than location; for Cd and chromium (Cr), location was more significant. Mean metal levels differed among species for all metals, except Cd. Highest levels were As (great black-backed gulls, Larus marinus), Cr (great egret, Ardea alba), Pb (Canada goose, Branta canadensis), and Hg and Se (black-crowned night heron, Nycticorax nycticorax). There were significant locational differences only for herring gulls (Larus argentatus); significant differences were found for all metals. Levels of Hg and Pb may be sufficiently high in eggs of some species to produce adverse effects in predators that eat them. The proportion of samples above 0.3 ppm Hg (U.S. Environmental Protection Agency [EPA] freshwater criteria for freshwater fish), the contaminant of health concern, ranged from 0% (Canada goose, great egret), to 14 and 27% in gulls, to 50% (black-crowned night heron). Some herring gull, great black-backed gull, and black-crowned night heron eggs had 0.5 ppm or higher Hg. Thus, human consumption of eggs may pose a risk to fetuses and young children.

Contaminant exposure of birds nesting in Green Bay, Wisconsin, USA.

In earlier studies, elevated concentrations of polychlorinated biphenyl (PCB) and p,p'-dichlorodiphenyldichloroethylene (DDE) were reported in double-crested cormorant (Phalacrocorax auritus) eggs and tree swallow (Tachycineta bicolor) eggs and nestlings collected from lower Green Bay (WI, USA) in 1994 and 1995 and black-crowned night-heron (Nycticorax nycticorax) eggs collected in 1991. Comparable samples collected in 2010 and 2011 indicated that concentrations of PCBs were 35%, 62%, 70%, and 88% lower than in the early 1990s in tree swallow eggs, tree swallow nestlings, double-crested cormorant eggs, and black-crowned night-heron eggs, respectively; concentrations of DDE were 47%, 43%, 51%, and 80% lower, respectively. These declines are consistent with regional contaminant trends in other species. Concentrations of PCBs were higher in herring gull (Larus argentatus) than in black-crowned night-heron eggs collected from Green Bay in 2010; PCB concentrations in double-crested cormorant and tree swallow eggs were intermediate. The estimated toxicity of the PCB mixture in eggs of the insectivorous tree swallow was the equal to or greater than toxicity in the 3 piscivorous bird species. A multivariate analysis indicated that the composition percentage of lower-numbered PCB congeners was greater in eggs of the insectivorous tree swallow than in eggs of the 3 piscivorous species nesting in Green Bay. Dioxin and furan concentrations and the toxicity of these chemicals were also higher in tree swallows than these other waterbird species nesting in Green Bay.

Organochlorine pesticides, polychlorinated biphenyls, metals, and trace elements in waterbird eggs, Salton Sea, California, 2004

The Salton Sea is a highly eutrophic, hypersaline terminal lake that receives inflows primarily from agricultural drainages in the Imperial and Coachella valleys. Impending reductions in water inflow at Salton Sea may concentrate existing contaminants which have been a concern for many years, and result in higher exposure to birds. Thus, waterbird eggs were collected and analyzed in 2004 and compared with residue concentrations from earlier years; these data provide a base for future comparisons. Eggs from four waterbird species (black-crowned night-heron [Nycticorax nycticorax], great egret [Ardea alba], black-necked stilt [Himantopus mexicanus], and American avocet [Recurvirostra Americana]) were collected. Eggs were analyzed for organochlorine pesticides, polychlorinated biphenyls (PCBs), metals, and trace elements, with current results compared to those reported for eggs collected from the same species and others during 1985–1993. The two contaminants of primary concern were p,p′-DDE (DDE) and selenium. DDE concentrations in night-heron and great egret eggs collected from the northwest corner of Salton Sea (Whitewater River delta) decreased 91 and 95%, respectively, by 2004, with a concomitant increase in eggshell thickness for both species. Decreases in bird egg DDE levels paralleled those in tissues of tilapia (Oreochromis mossambicus × O. urolepis), an important prey species for herons and egrets. Despite most nests of night-herons and great egrets failing in 2004 due to predation, predicted reproductive effects based on DDE concentrations in eggs were low or negligible for these species. The 2004 DDE findings were in dramatic contrast to those in the past decade, and included an 81% decrease in black-necked stilt eggs, although concentrations were lower historically than those reported in night-herons and egrets. Selenium concentrations in black-necked stilt eggs from the southeast corner of Salton Sea (Davis Road) were similar in 1993 and 2004, with 4.5–7.6% of the clutches estimated to be selenium impaired during both time periods. Because of present selenium concentrations and future reduced water inflow, the stilt population is of special concern. Between 1992 and 1993 and 2004 selenium in night-heron and great egret eggs from the Whitewater River delta at the north end of the Sea decreased by 81 and 55%, respectively. None of the night-heron or egret eggs collected in 2004 contained selenium concentrations above the lowest reported effect concentration (6.0 μg/g dw). Reasons for selenium decreases in night-heron and egret eggs are unknown. Other contaminants evaluated in 2004 were all below known effect concentrations. However, in spite of generally low contaminant levels in 2004, the nesting populations of night-herons and great egrets at Salton Sea were greatly reduced from earlier years and snowy egrets (Egretta thula) were not found nesting. Other factors that include predation, reduced water level, diminished roost and nest sites, increased salinity, eutrophication, and reduced fish populations can certainly influence avian populations. Future monitoring, to validate predicted responses by birds, other organisms, and contaminant loadings associated with reduced water inflows, together with adaptive management should be the operational framework at the Salton Sea.

Mercury and Drought Along the Lower Carson River, Nevada: I. Snowy Egret and Black-Crowned Night-Heron Annual Exposure to Mercury, 1997–2006

The dynamic nature of the annual volume of water discharged down the Carson River over a 10-year period, which included a century flood and drought, was examined in order to gain a better understanding of mercury movement, biological availability, and exposure to waterbirds nesting at Lahontan Reservoir. Total annual water discharge directly influenced total mercury (THg) in unfiltered water above the reservoir and downstream of a mining area, whereas methyl mercury (MeHg) at the same site was negatively related to annual discharge. Annual water storage at Lahontan Reservoir in the spring and early summer, as expected, was directly related to annual Carson River discharge. In contrast to the findings from above the reservoir, annual MeHg concentrations in water sampled below the reservoir were positively correlated with the total discharge and the amount of water stored in the reservoir on 1 July; that is, the reservoir is an important location for mercury methylation, which agrees with earlier findings. However, unfiltered water MeHg concentrations were about 10-fold higher above than below the reservoir, which indicated that much MeHg that entered as well as that produced in the reservoir settled out in the reservoir. Avian exposure to mercury at Lahontan Reservoir was evaluated in both eggs and blood of young snowy egrets (Egretta thula) and black-crowned night-herons (Nycticorax nycticorax). Annual MeHg concentrations in unfiltered water below the reservoir, during the time period (Julian Days 90-190) when birds were present, correlated significantly with mercury concentrations in night-heron blood (r(2 )= 0.461, p = 0.027), snowy egret blood (r(2 )= 0.474, p = 0.024), and night-heron eggs (r(2) = 0.447, p = 0.029), but not snowy egret eggs. A possible reason for lack of an MeHg water correlation with snowy egret eggs is discussed and relates to potential exposure differences associated with the food habits of both species. THg concentrations in water collected below the reservoir were not related to egg or blood mercury concentrations for either species.

Effects of Contaminants on Reproductive Success of Aquatic Birds Nesting at Edwards Air Force Base, California

Contamination by organochlorine pesticides (OCs), polychlorinated biphenyls, metals, and trace elements at Edwards Air Force Base (EAFB), located in the Mojave Desert, could adversely affect nesting aquatic birds, especially at the sewage lagoons that comprise Piute Ponds. Estimates of avian reproduction, in conjunction with analyses of eggs and avian foods for contaminant residues, may indicate the potential for negative effects on avian populations. From 1996 to 1999, we conducted studies at the Piute Ponds area of EAFB to evaluate the impacts of contaminants on nesting birds. Avian reproduction was evaluated in 1999. Eggs were collected for chemical analyses in 1996 and 1999, and African clawed frogs (Xenopus laevis), a likely food source, were collected for chemical analyses in 1998. Avian species occupying the higher trophic levels--black-crowned night-heron (Nycticorax nycticorax), white-faced ibis (Plegadis chihi), and American avocet (Recurvirostra americana)--generally bioaccumulated higher concentrations of contaminants in their eggs. Reproductive success and egg hatchability of night-herons and white-faced ibises in the Piute Ponds were similar to results observed at other western colonies. Deformities were observed in only one embryo in this study, but concentrations of contaminants evaluated in this ibis embryo were considered insufficient to have caused the deformities. Because clawed frogs, a primary prey item for night-herons at Piute Ponds, had no detectable levels of any OCs, it is likely that OCs found in night-heron eggs were acquired from the wintering grounds rather than from EAFB. The presence of isomers of dichlorodiphenyltrichloroethane (DDT) in ibis eggs indicated recent exposure, but invertebrates used for food by ibises were not sampled at Piute Ponds, and conclusions about the source of OCs in ibis eggs could not be made. Concentrations of contaminants in random and failed eggs of individual species were not different, and we concluded that contaminants did not cause the observed egg failures.

Organochlorines in black-crowned night heron (Nycticorax nycticorax) eggs reflect persistent contamination in northeastern US estuaries.

Colonially nesting aquatic birds can indicate site-specific contamination because they are high-level predators and dependent upon local resources during the breeding season. We compared persistent organochlorine concentrations in black-crowned night heron (Nvcticorax nvcticorax) eggs collected in 1992-93 among areas of known contamination (New York Harbor, Boston Harbor, Cape Cod, and Delaware Bay) and a reference site (Nantucket Island) in the northeastern United States (7-16 eggs/site). Total PCBs, p,p'-DDE, oxychlordane, heptachlor epoxide, and trans-nonachlor were detected in most eggs and were significantly higher in New York Harbor compared to all other sites (ANOVA on factor scores from the first principal component, which accounted for 75% of data variance, p < 0.0001). New York also had the highest contamination index (the number of Superfund sites within 20 km of the colony) among the sites. Nantucket, the reference site, had consistently low contamination in eggs, and the lowest contamination index. We conclude that black-crowned night heron eggs are useful indicators of site-specific persistent organochlorine contamination, as indexed by the number of nearby Superfund sites.

Reproductive Success of the Black-crowned Night Heron at Alcatraz Island, San Francisco Bay, California, 1990-2002

Abstract Nesting chronology, habitat use, subcolony use, and hatchability were documented for the Black-crowned Night Heron (Nycticorax nycticorax) nesting at Alcatraz Island, San Francisco Bay, California during 1990-2002. Reproductive success was estimated using the Mayfield method and compared among years. Totals of monitored nests per year ranged from 68 in 2001 to 341 in 1996, with a trend of declining numbers since 1996. An increase in numbers of the Western Gull (Larus occidentalis), the Black-crowned Night Heron’s primary competitor, occurred during the same period. Overall reproductive success of the Black-crowned Night Heron at Alcatraz Island was below the 13-year average of 56.4% since 1996. During the study, the average number of chicks fledged per nest each year ranged from 0.46 to 1.27, which is less than the two chicks per nest suggested as a requirement for a sustained population. Embryos in five of 187 failed Black-crowned Night Heron eggs were deformed. In 1990 and 1991, eggs were analy...

Spatial and temporal trends of contaminants in eggs of wading birds from San Francisco Bay, California

AbstractBetween 1989 and 1991, reproduction by black‐crowned night‐herons (Nycticorax nycticorax) and snowy egrets (Egretta thula) was studied at sites in San Francisco Bay. Eggs were collected from these and other bay sites and from South Wilbur Flood Area, a reference site in California's San Joaquin Valley. Eggs were analyzed for inorganic trace elements, organochlorine pesticides, and polychlorinated biphenyls (PCBs). Results were compared among sites and years and with results of previous studies. There was some evidence of impaired reproduction, but concentrations of contaminants were generally lower than threshold levels for such effects. Egg hatchability was generally good, with predation being the factor that most limited reproductive success. Mean PCB concentrations were generally higher in eggs from the south end of San Francisco Bay than from the north, but the only temporal change, an increase, was observed at Alcatraz Island. There were spatial differences for p,p′‐DDE in night‐heron eggs in 1990, but the highest mean concentration of DDE was in night‐heron eggs from South Wilbur in 1991. Temporal declines in maximum concentrations of DDE in eggs were observed in the bay, but means did not change significantly over time. At Bair Island in the southern end of the bay, mean concentrations of mercury decreased while selenium increased in night‐heron eggs over time, but there were no clear bay‐wide spatial or temporal trends for either element.

Transfer and accumulation of organochlorines from black‐crowned night‐heron eggs to chicks

AbstractEggs and sibling 1‐, 3‐, and 5‐d‐old chicks from seven black‐crowned night‐heron (Nycticorax nycticorax) broods were collected from Green Bay, Wisconsin, and analyzed for organochlorines. The concentration (μg/g) of nine organochlorines either decreased (n = 7) or remained the same (n = 2) as the chicks grew older. In contrast, the total mass (μg) of these nine organochlorines increased (n = 7) or remained the same (n = 2) as the chicks grew older. Accumulation rates of mass (μg/d) between egg and 5‐d‐old chicks for each of the nine organochlorines were positive and varied from 0.2 μg/d (p,p′‐DDT) to 42 μg/d (PCBs). These results suggest that the loss of contaminant mass from eggs to chicks reported in some earlier studies was because the entire carcass was not analyzed (i.e., not including skin, gastrointestinal tract, etc.). These results also support the use of contaminant accumulation rates as an indicator of local contamination.

Green Coloration of Recently Laid Black-Crowned Night-Heron Eggs

-Color of Black-crowned Night-Heron (Nycticorax nycticorax) eggs of known laying order was quantified in a Wisconsin colony. Eggs were of greener intensity on the day of laying and one day thereafter than earlier laid eggs within a clutch. Egg color can be used to identify clutches within one day after clutch completion and to assign laying order within clutches during this period. Received 15 January 1991, accepted 18 March 1991.

Organochlorines and selenium in California night-heron and egret eggs

Exceptionally high concentrations of DDE were found in black-crowned night-heron (Nycticorax nycticorax) (geometric mean 8.62 μg g(-1) wet wt.) and great egret (Casmerodius albus) (24.0 μg g(-1)) eggs collected from the Imperial Valley (Salton Sea), California in 1985. DDE concentrations in 14 of the 87 (16%) randomly selected night-heron eggs from six colonies (two in San Francisco Bay, three in the San Joaquin Valley, and one at Salton Sea) were higher than those associated with reduced reproductive success of night-herons (8 μg g(-1)). In addition, mean shell thickness of night-heron eggs collected from the San Joaquin Valley and from San Francisco Bay during 1982-1984 was significantly less than pre-DDT thickness and was negatively correlated (r=-0.50, n=75, P<0.0001) with DDE concentration. Mean selenium concentration in night-heron eggs from Salton Sea (1.10 μg g(-1)) was significantly higher than in eggs from three locations in the San Joaquin Valley, and in egret eggs from Salton Sea.

Organochlorines and Mercury in Eggs of Coastal Terns and Herons in California, USA

-In San Francisco Bay, California, USA, concentrations of DDE and mercury in eggs differed among Caspian Tern, Forster's Tern, Black-crowned Night-Heron, and Snowy Egret in 1982. Geometric mean DDE concentrations were higher (P <0.05) in Caspian Tern eggs (6.93 ppm, wet weight) than in eggs of other species (1.92 2.84 ppm). Mean mercury concentrations were significantly greater in Caspian Tern (1.25 ppm) and Forster's Tern (0.90 ppm) eggs than in night-herons (0.41 ppm), but night-heron eggs contained higher concentrations of mercury than did the eggs of Snowy Egrets (0.21 ppm). There were no significant differences among species for mean concentrations of trans-nonachlor or PCBs; other organochlorines occurred in fewer than half of the samples, so means were not compared. Caspian Tern eggs from San Francisco Bay had higher PCB concentrations (4.85 ppm) than did eggs of this species from San Diego Bay, California (1.70 ppm) or Elkhorn Slough, California (1.83 ppm), but we detected no significant differences in mean concentrations of other organochlorines. DDE concentrations in 5 of 47 (10.6%) nightheron eggs from San Francisco Bay exceeded 8 ppm, a level associated with impaired reproduction in this species. DDE concentrations were negatively correlated with eggshell thickness in night-herons and egrets.

Association between PCBs and lower embryonic weight in black‐crowned night herons in San Francisco bay

Reproductive problems, including congenital malformations, reduced hatching success, and decreased survival of hatchlings, have been observed in colonial-nesting water birds at the San Francisco Bay National Wildlife Refuge (SFBNWR). Twenty-four black-crowned night heron (Nycticorax nycticorax) eggs were collected from SFBNWR in 1983. Twelve of these were collected from separate nests when late-stage embryos were pipping, and an additional egg was randomly collected from each nest for organochlorine analysis. Overt anomalies and skeletal defects were not apparent. Embryonic weights (with partially absorbed yolk sacs removed) were 15% lower (p less than 0.05) in SFBNWR embryos compared to control embryos from the Patuxent Wildlife Research Center (PWRC). Crown-rump length and femur length were shorter for SFBNWR embryos. The geometric mean polychlorinated biphenyl (PCB) concentration in SFBNWR eggs was 4.1 ppm wet weight, with a range of 0.8-52.0 ppm. A negative correlation (r = -0.61; p less than 0.05) existed between embryonic weight and log-transformed PCB residues in whole eggs collected from the same nest at SFBNWR, suggesting a possible impact of PCBs on embryonic growth. A correlation with embryonic weight did not occur for DDE [1,1-dichloro-2,2-bis(p-chlorophenyl) ethylene] residues. Liver microsomal aryl hydrocarbon hydroxylase activity was neither significantly elevated nor correlated with PCB, DDE, or PCB plus DDE log-transformed residues. It is unknown whether the apparent association between PCBs and lower weight is persistent through hatching.

Current Impact of DDE on Black-Crowned Night-Herons in the Intermountain West

Organochlorine contamination was studied in eight black-crowned night-heron (Nycticorax nycticorax) populations nesting in Washington, Oregon, and Nevada in 1978-80. DDE was detected in 220 eggs sampled; eggshell thickness was negatively correlated with residues of DDE and polychlorinated biphenyls (PCB's). Other contaminants were detected in 35% or fewer of the eggs. Except for the two Columbia River colonies in which local DDE contamination was a probable compounding factor, a strong north-south clinal pattern of DDE residues among colonies existed. Southern colonies were most contaminated, and observed productivity was below population maintenance in one colony (Ruby Lake). At DDE levels in eggs above 8 ppm, clutch size and productivity decreased, and the incidence of cracked eggs increased. No evidence of breeding-ground DDE-DDT contamination was found except along the Columbia River. J. WILDL. MANAGE. 48(1):1-13 DDT and its metabolites may have played a role in the decline of blackcrowned night-herons in the northeastern United States (Ohlendorf et al. 1978). For example, Anderson and Hickey (1972) reported 18% shell thinning by 1952 in New Jersey. The use of DDT in the United States was banned in 1972, and by the midto late 1970's, production rates at several locations in eastern North America (Wisconsin, Qu6bec, Rhode Island, and Massachusetts) appeared normal, eggshell thickness was normal or near-normal, and/ or residues of organochlorine pesticides were lower (Hoefler 1980, Tremblay and Ellison 1980, Custer et al. 1983), with the possible exception being Lake Ontario (Price 1977). Similar improvements have been reported for other species, including the osprey (Pandion haliaetus) (Henny 1977, Spitzer et al. 1978). An adult black-crowned night-heron died at Ruby Lake National Wildlife Refuge near Elko, Nev., in 1975-3 years after the DDT ban. Its brain contained 230 ppm DDE and 1.1 ppm DDT (wet weight) (Ohlendorf et al. 1979). Although DDE concentrations in eggs from the northeast were diminishing in the late 1970's, the mortality at Ruby Lake in 1975 led us to suspect that DDE was still a problem for bird populations in the Intermountain West. Most birds nesting in the region are migratory, and many winter in Latin America. We studied two colonies in 1978 and extended our efforts to eight colonies by 1980. This study was designated to (1) determine levels of DDE and other organochlorine residues in black-crowned nightheron eggs; (2) evaluate the most common contaminants in relation to shell thickness and egg breakage; (3) evaluate regional patterns and trends of DDE residues; (4) evaluate DDE in eggs in relation to productivity; (5) evaluate the source of the contaminants; and (6) evaluate residues in herons found dead to determine if pesticides were implicated. National Wildlife Refuge personnel who provided field assistance included S. H. Bouffard, J. E. Cornely, and S. P. ThompJ. Wildl. Manage. 48(1):1984 1 This content downloaded from 40.77.167.80 on Mon, 13 Jun 2016 06:09:49 UTC All use subject to http://about.jstor.org/terms 2 DDE IN BLACK-CROWNED NIGHT-HERONS * Henny et al.

Population Trends and Environmental Contaminants in Herons in the Tennessee Valley, 1980-81

-Great Blue Heron (Ardea herodias) eggs (N = 40) collected in 1980 from four of the largest colonies in the Tennessee Valley contained organochlorine pesticide, polychlorinated biphenyl (PCB), and chromium concentrations below those associated with reduced productivity. Low concentrations of organochlorine pesticide and PCB residues also were found in eggs (N = 31) from three of the larger Black-crowned Night-Heron (Nycticorax nycticorax) colonies in the Tennessee Valley. However, DDE concentrations in two of the Black-crowned Night-Heron eggs exceeded levels associated with reduced nesting success. Mercury was found in all eggs of both species with residues ranging to a high of 2.0 ppm; residues of this magnitude have not been identified as reducing nesting success of either of these two species. Green-backed Heron (Butorides striatus) eggs collected in 1981 near a former DDT manufacturing site in the Tennessee Valley had the highest DDE concentrations ever reported for this species; the effect of these high concentrations on productivity is not known. Eggshell thickness of Great Blue Heron, Green-backed Heron, and Black-crowned NightHeron eggs averaged 7.5%, 7.6%, and 3% thinner, respectively, than shell thickness of eggs collected before 1947. This amount of thinning is not deleterious; intraclutch variation in shell thickness is often this high. Shell thickness in all three species was correlated (P < 0.1) with DDE concentrations. The number of nesting pairs of Great Blue Herons and Black-crowned Night-Herons at each of the colonies studied has been stable or increasing during the previous decade. These population data, combined with the residue data, suggest that organochlorine pesticides, PCBs, mercury, and chromium are not adversely affecting these populations. However, we did not assess nesting success which would be a requisite for confirming this.

Organochlorine Residues in Atlantic Coast Black-Crowned Night-Heron Eggs, 1979

-Eggs of Black-crowned Night-Herons (Nycticorax nycticorax) were collected in 1979 from two colonies in North Carolina, two colonies in Rhode Island, and one colony in Massachusetts. Mean concentrations of DDE and PCBs were higher in the New England samples than in those from North Carolina. Mean concentrations of organochlorine contaminants in addled eggs collected at two New England colonies were not significantly different from those in randomly selected eggs. In comparison with the results of a similar study conducted in 1973 at two of the New England colonies, concentrations of DDE in night-heron eggs were lower in 1979, DDD was less frequently detected, DDT and dieldrin were less frequently detected in eggs from one of the two colonies, and eggshell thickness was greater in 1979 for one of the colonies. PCB concentrations in eggs from one colony were lower in 1979 than 1973.