The study examined the impact of drinking water source on disease transmission among pastoralists, using a case study of the Ngorongoro Conservation Area (NCA) in Tanzania. A total of four villages located in the NCA were sampled and studied, namely: Endulen, Nainokanoka, Kayapus and Meshili. This was a cross-sectional study which deployed questionnaires, direct observation, document reviews and focus group discussions. A total of 396 households participated in the survey. The collected data were analyzed using qualitative data analysis techniques and descriptive statistics such as frequencies. The study findings revealed that unfiltered waterholes shared among humans, livestock and wildlife accounted for 82.1%; indicating that it was the primary source of disease transmission. Other sources of disease transmissions were: the use of untreated tap water, rivers, spring water and rainwater. The factors influencing the use of unfiltered waterholes were limited access to tap water, scarcity of drinking water sources, drought, population increase and tourists. The co-usage of water coupled with lack of latrines suggest fecal contamination of drinking water sources. Women were more susceptible to waterborne diseases as they were more directly engaging in all domestic activities including fetching for water as compared to men. The waterborne diseases affecting pastoralist communities were diarrhea, cholera, skin infection, dysentery, worms and typhoid fever. The study concluded that there is a nexus between shared drinking water sources and the prevalence of high anthrax (Bacillus anthracis) infection rates. It is recommended that the adverse impacts of disease transmission on drinking water sources can be mitigated through increasing supply of tap water, using treated water and encouraging community use of pit latrines.