New Species Of Culicoides Research Articles

New species of Culicoides, description of female of Culicoides pseudocrescentis Tavares & Luna Dias and new record of Culicoides castillae Fox from Southeastern Brazil (Diptera: Ceratopogonidae)

A new species of Culicoides, C. andradinensis Felippe-Bauer & Santarém is described and illustrated based on female specimens from the municipality of Andradina, São Paulo state, Brazil. The new species is placed in the limai species group and compared with their related congeners. The description of females of Culicoides pseudocrescentis Tavares & Luna Dias based on specimens from Brazilian states of Espírito Santo and São Paulo is given. Culicoides castillae Fox is first recorded from Brazil. All studied specimens are deposited in the Ceratopogonidae Collection of Fundação Oswaldo Cruz, Brazil (Fiocruz/CCER).

Culicoides jiangchengensis, a new species of the subgenus Sinocoides (Diptera, Ceratopogonidae) based on integrative taxonomy from China.

Biting midges of the genus Culicoides are important in both medicine and veterinary medicine because their blood-feeding regime enable them to transmit a variety of pathogens. In this study, the morphological characteristics of the new species of Culicoides (Sinocoides) jiangchengensis Wang et Liu sp. nov are described and compared with the other species of female Culicoides in the subgenus Sinocoides. Three morphological characteristics of C. jiangchengensis, such as without sensory pit in 3rd palpus segment, sensilla coeloconica on flagellomeres 1,9-13, and m1 and m2 cell of the wings with pale spots, were different from the other nine species of culicoides in subgenus Sinocoides. Genetically, C. jiangchengensis are most closely related to C. malipoensis, but they were located in different branches and the minimum interspecific distance between them was 12.6%. In addition, a checklist of 10 species in the subgenus Sinocoides Chu, 1983 (Diptera: Ceratopogonidae: Culicoides) in China, including the new species C. jiangchengensis Wang et Liu sp. nov., is provided, and an updated key to species of the subgenus Sinocoides Chu, 1983 was presented.

Delineation of Culicoides species by morphology and barcode exemplified by three new species of the subgenus Culicoides (Diptera: Ceratopogonidae) from Scandinavia.

BackgroundCulicoides biting midges (Diptera: Ceratopogonidae) cause biting nuisance to livestock and humans and are vectors of a range of pathogens of medical and veterinary importance. Despite their economic significance, the delineation and identification of species where only morphology is considered, as well as the evolutionary relationships between species within this genus remains problematic. In recent years molecular barcoding has assisted substantially in the identification of biting midges in the multiple entomological survey projects which were initiated in many European countries following the bluetongue outbreak in 2006–2009. These studies revealed potentially new species and “species-complexes” with large genetic and morphological variability. Here we use molecular barcoding, together with morphological analysis, to study subgenus Culicoides Latreille from Scandinavia with focus on three potentially new species.MethodsBiting midges were collected at various sites in Denmark and Sweden. Culicoides specimens were described by variation of a fragment of their cytochrome c oxidase subunit 1 (COI) gene sequence and wing, palp and antennal characters.ResultsIt is shown that three new species initially separated by DNA barcoding with mitochondrial COI can be distinguished by morphological characters. In this context a key to Scandinavian subgenus Culicoides using wing and maxillary palp characters is presented. The key is including the three new species Culicoides boyi, Culicoides selandicus and Culicoides kalix.ConclusionThree new species of Culicoides biting midges were identified and could be identified by both molecular and morphological differences. Evaluation of differences between and within taxa of biting midges using COI barcode yielded a rough estimate of species delineation; interspecies differences across Culicoides subgenera approaches 20%, whereas intraspecies differences are below 4% and in most cases below 1%.Electronic supplementary materialThe online version of this article (doi:10.1186/s13071-015-0750-4) contains supplementary material, which is available to authorized users.

Two new species and new records of biting midges of the genus Culicoides from northwestern Argentina (Diptera: Ceratopogonidae)

The following two new species of Culicoides from the Argentinean Yungas are described, illustrated and placed to subgenus or species group and compared with related congeners: Culicoides calchaqui Spinelli & Veggiani Aybar and Culicoides willinki Spinelli & Veggiani Aybar. Culicoides daedaloides Wirth & Blanton is recorded for the first time for Argentina and Culicoides pseudoheliconiae Felippe-Bauer is firstly mentioned from the northwestern region of the country.

Two new species and new records of biting midges of the genus Culicoides from northwestern Argentina (Diptera: Ceratopogonidae)

The following two new species of Culicoides from the Argentinean Yungas are described, illustrated and placed to subgenus or species group and compared with related congeners: Culicoides calchaqui Spinelli & Veggiani Aybar and Culicoides willinki Spinelli & Veggiani Aybar. Culicoides daedaloides Wirth & Blanton is recorded for the first time for Argentina and Culicoides pseudoheliconiae Felippe-Bauer is firstly mentioned from the northwestern region of the country.

Description of Culicoides lisicarruni (Diptera: Ceratopogonidae), a new species from Cundinamarca, Colombia

A new species of Culicoides of the subgenus Diphaomyia Vargas from high altitudes of the Andes in Colombia is described and photographied. The species is compared with its similar congener Culicoides marinkellei Wirth & Lee. Data on the collecting site and notes on the species daily activity are also provided.

Five new species of Culicoides Latreille described from Colombia, yielding a new species list and country records (Diptera: Ceratopogonidae)

The following five new species of Culicoides from Colombia are described, illustrated and placed to subgenus or species group: Culicoides antioquiensis, Culicoides gabrieli, Culicoides inermis, Culicoides micayensis and Culicoides nigrifemur. C. gabrieli is also known from Peru. When possible, their position in previously published keys is indicated and their features discussed in light of the most recent revisions. A list of 180 Culicoides species known (114) or suspected of being in Colombia (66) is given in a Table. Of these, 12 including the new species are recorded from Colombia for the first time.

Culicoides huaynacapaci, a new species from the Department of Cajamarca, Peru (Diptera, Ceratopogonidae)

A new species of Culicoides of the subgenus Mataemyia Vargas, Culicoides huaynacapaci Felippe-Bauer, is described and illustrated based on female and male specimens collected biting humans in Department of Cajamarca, in Peruvian Amazonia. The new species is compared with its similar congener C. albuquerquei Wirth & Blanton.

Orbiviruses in the Mediterranean Basin: Updated Epidemiological Situation of Bluetongue and New Methods for the Detection of BTV Serotype 4

Bluetongue virus (BTV), a member of the Orbivirus genus, has contributed to great economical losses in countries across the Mediterranean basin. Although BTV has an African origin, it has been reported in the South of Europe since 1924 when it was first detected in Cyprus. After this first Bluetongue (BT) outbreak, many others followed in most Mediterranean countries, resulting in seven BTV serotypes detected in 17 countries in Central-, South-Europe and North Africa in the last 10 years. Currently, six BTV serotypes (1, 2, 4, 8, 9 and 16) are circulating throughout Central- and Western Europe. The unexpected occurrence of BTV serotype 8 in Central Europe in 2006 and its spread and persistence have evidenced changes in the BTV scenario: new serotypes, never detected in the Mediterranean area so far, are playing a central role in the maintenance of BTV in Europe, and new species of Culicoides are now confirmed to be able to transmit the virus. Therefore, it is necessary to improve and implement specific assays in order to identify the serotypes currently present in different Mediterranean countries in a fast and reliable way. In this study, we present a new gel-based and real-time RT-PCR assay for the detection of BTV serotype 4. The sequence amplified in this test is located within BTV segment 2, a variable region of BTV dsRNA genome encoding the major outer-capsid protein VP2. No cross-reaction has been shown with other genetically or geographically related viruses and the sensitivity of this test allows the detection of 1.5-15 TCID50/ml of BTV.

A new Culicoides (Diptera:Ceratopogonidae) of the subgenus Diphaomyia from Peru

A new species of Culicoides of the subgenus Diphaomyia, Culicoides jurbergi Felippe-Bauer, is described and illustrated based on female specimens collected biting man and with light traps in Peruvian Amazonia. The species is compared with its similar congener mirsae Ortiz.

Two new species of subgenus Avaritia of the Culicoides biting midges (Diptera : Ceratopogonidae) from Japan

Two new species of Culicoides biting midges belonging to the subgenus Avaritia, the female of C. nupurius sp. n. from Tokachi and Kamikawa districts, Hokkaido and the male of C. hasegawai sp. n. from Iwate prefecture, Honshu are described and illustrated.

DESCRIPTION OF A NEW SPECIES OF THE GENUS CULICOIDES (DIPTERA: CERATOPOGONIDAE) FROM XINGJUNG UYGUR AUT. REG. CHINA*

Abstract A new species of Culicoides, C. tahemanensis sp. nov. is described from Xingjiang Uygur Aut. Reg. in China. The new species is closely allied to Culicoides grisescens Edwards, but both of them are distinctly different in pale spots of cell R5 and cell A of wing of female, and shape of distal portion of aedeagus of male. The ninth tergum of new species is somewhat allied to C. nipponensis Tokunaga, but distinctly different in pale spots of cell R5, cell M2 and cell A of wing, and shape of aedeagus of male. The type speciemens are deposited in the Institute of Military Medical Sciences, Shenyang Military District, Shengyang 110034, China.

TWO NEW SPECIES AND THREE NEW RECORDS OF THE GENUS CULZCOIDES (DIPTERA:CERATOPOGONIDAE) IN CHINA

Abstract Two new species of Culicoides, C. desytoculus sp. nov. and C. pulchellus sp. nov., are described from China. Three species of Culicoides, C. gynznopterus, C. parabarnetti and C. pikongkoi, are recorded for the first time in China.

TWO NEW SPECIES OF CULICOIDES FROM HAINAN PROVINCE, CHINA (DIPTERA: CERATOPOGONIDAE)

Abstract Two new species of Culicoides from Hainan Province, C. jianfenglingensis and C.cleaves, are described.

New species and new records ofCulicoides(Diptera, Ceratopogonidae) from Lesbos and Rhodes, Greece

Descriptions of three new species of Culicoides from Rhodes, Greece, and notes on four other species from the Islands of Rhodes and Lesbos.

New species of Culicoides biting midges (Diptera: Ceratopogonidae) from Colombia.

Eight new species of Culicoides biting midges are described and figured from material collected during a survey of bloodsucking insects on the Rio Raposo, Valle, Colombia: Culicoides beaveri, C. dalessandroi, C. eldridgei, C. raposoensis, C. sanmartini, C. teretipalpis, C. traPidoi , and C. youngi .

A new biting midge from Japan and Korea (Diptera, Heleidae)

During the course of bionomic studies being conducted on mosquitoes by members of the staff of the Department of Entomology, 406th Medical General Laboratory, a new species of Culicoides was collected from three localities in central Honshu, Japan and one locality in South Korea. This report includes a description of the adults of the new gnat and brief notes on its biology. Morphological interpretations are basically those of Wirth (1952). Measurement of length is the combined length of thorax and abdomen. The costal ratio is the combined length of thorax and abdomen. The costal ratio is obtained by dividing the costal length (from the arculus to the distal edge of the second radial cell) by the wing length (from the arculus to the wing tip). Measurements were made on paratype specimens mounted in either balsam or synthetic resin.

A New Species of Culicoides from Florida with Additional Distribution Data for the Genus (Diptera: Heleidae)

In the course of examining mosquito light trap material at the Florida State Board of Health, Bureau of Entomology, a new species of Culicoides has been found and four other species taken which are new records for the State. Type specimens of the new species are deposited in the United States National Museum in Washington, D. C., and paratypes are in the University of Florida Collection, Gainesville.

Two New Species of Anthropophilic Culicoides from Guatemala (Diptera: Heleidae)

The purpose of this short paper is to make available names for two new species of Culicoides which have been submitted by Dr. Colvin L. Gibson, of the Laboratory of Tropical Diseases, National Institutes of Health, while assigned to the Pan American Sanitary Bureau in Guatemala. These species, along with C. guttatus (Coquillett) and C. paraensis (Goeldi), were collected in the area around San Pedro Yepocapa, Department of Chimaltenango, Guatemala, while biting man, and are the subject of a separate report by Dr. Gibson regarding their possible role in the transmission of human onchocerciasis in Guatemala.