Activation Analysis Research Articles

Targeting PPARγ via SIAH1/2-mediated ubiquitin-proteasomal degradation as a new therapeutic approach in luminal-type bladder cancer

Bladder cancer (BC) is the second most prevalent genitourinary malignancy worldwide. Despite recent approvals of immune checkpoint inhibitors and targeted therapy for muscle invasive or recurrent BC, options remain limited for patients with non-muscle invasive BC (NMIBC) refractory to Bacillus Calmette-Guérin (BCG) and chemotherapy. NMIBC is more frequently classified as a luminal subtype, in which increased PPARγ activity is a key feature in promoting tumor growth and evasion of immunosurveillance. Cinobufotalin is one of the major compound of bufadienolides, the primary active components of toad venom that has been utilized in the clinical treatment of cancer. We herein focused on cinobufotalin, examining its anticancer activity and molecular mechanisms in luminal-type NMIBC. Our results newly reveal that cinobufotalin strongly suppresses the viability and proliferation of luminal BC cells with minimal cytotoxic effects on normal uroepithelial cells, and exhibits significant antitumor activity in a RT112 xenograft BC model. Mechanistically, our sub-G1-phase cell accumulation, Annexin V staining, caspase-3/8/9 activation, and PARP activation analyses show that cinobufotalin induces apoptosis in luminal-type BC cells. Cinobufotalin significantly inhibited the levels of PPARγ and its downstream targets, as well as lipid droplet formation and free fatty acid levels in RT112 cells. PPARγ overexpression rescued RT112 cells from cinobufotalin-induced apoptosis and mitigated the downregulation of FASN and PLIN4. Finally, we show seemingly for the first time that cinobufotalin promotes SIAH1/2-mediated proteasomal degradation of PPARγ in luminal BC cells. Together, these findings compellingly support the idea that cinobufotalin could be developed as a promising therapeutic agent for treating luminal-type NMIBC.

Zoning of Trace Metals Sediments, in the Lagoa do Carro-PE Dam, From TEL/ PEL and ERL/ ERM Parameters

Objective: The objective of this study is to analyze the presence of trace metals (Cd, Hg, Pb, Zn, Cr and Ni), using the TEL and PEL and ERL and ERM parameters of the Environmental Agencies of Canada and the United States, as well as CONAMA Resolution 357/05, in the bottom sediments of the Lagoa do Carro Dam, in the state of Pernambuco. Theoretical Framework: Considered one of the five largest water reservoirs in the state of Pernambuco, located in the Médio Capibaribe microbasin and responsible for supplying a population of approximately 300 thousand inhabitants in the municipalities of Carpina, Feira Nova, Glória do Goitá, Lagoa de Itaenga, Lagoa do Carro and Limoeiro. Method: The methodology adopted for this research includes obtaining the results using FUS-ICP (Fusion Inductively Coupled Plasma), where an oxidized sample is dissolved in a borate stream and then diluted in aqueous nitric acid; TD-ICP (Total Digestion Inductively Coupled Plasma and INAA (Instrumental Neutron Activation Analysis). Results and Discussion: The results obtained demonstrate that two of the metals analyzed are above those recommended by the WHO and pose risks to the dam's biota, as well as to users of the supply system. The numbers are well above acceptable limits, requiring urgent measures to maintain the status quo. Research Implications: The practical and theoretical implications of this research are discussed, providing insights into how the results can be applied or influence practices in the field of Geography and water reservoirs. Originality/Value: This study contributes to the literature by standing out for the originality of the research, whether through an innovative approach, new discoveries or practical contributions. The relevance and value of this research are evidenced by analyzing one of the five largest water reservoirs in the state of Pernambuco.

Live Cell Imaging of Human Liver Fibrosis using Hepatic Micro-Organoids.

Due to the limitations of available in vitro systems and animal models, we lack a detailed understanding of the pathogenetic mechanisms and have minimal treatment options for liver fibrosis. Therefore, we engineered a live cell imaging system that assesses fibrosis in a human multi-lineage hepatic organoid in a microwell (i.e., microHOs). Transcriptomic analysis revealed that TGFβ1 converted mesenchymal cells in microHOs into myofibroblast-like cells resembling those in fibrotic human liver tissue. When pro-fibrotic intracellular signaling pathways were examined, the anti-fibrotic effect of receptor-specific tyrosine kinase inhibitors was limited to the fibrosis induced by the corresponding growth factor, which indicates their anti-fibrotic efficacy would be limited to fibrotic diseases solely mediated by that growth factor. Based upon transcriptomic and transcription factor activation analyses in microHOs, GSK3β and p38 MAPK inhibitors were identified as potential new broad-spectrum therapies for liver fibrosis. Other new therapies could subsequently be identified using the microHO system.

Elemental profile of Ipê species to support timber tracing in Amazon Rainforest

Accurate identification of geographical origin and species of wood is crucial for endorsing laws to combat illegal trade. Here, the multi-element profile of Ipê species of economic interest in Brazil was investigated by neutron activation analysis (NAA). Ba, Br, Ca, Ce, Co, Eu, Fe, K, La, Na, Rb, Sc, Sm, Sr and Zn were determined in the heartwood and sapwood tissues. The Random Forest algorithm identified Sr, Ba, Sm, La, Ce and Br as the most important elements for discriminating species. The analyses revealed significant differences between species and wood tissues, indicating that multi-element profile has great potential as a forensic tool. The establishment of comprehensive reference databases that cover large geographical areas and take into account the natural variability between species is fundamental to achieving this goal.

Design and optimization of a source (reflector/shielding) performance test system based on (241Am-Be)-paraffin thermal neutron irradiation device

Laboratory measurements and Monte Carlo simulations were conducted to investigate the feasibility of employing a (reflector/shield) to improve the efficiency of a thermal neutron irradiator utilizing a paraffin-moderated single 241Am-Be source with an activity of 1 Ci. A multi-parameter optimization was performed to evaluate the potential of natural lead (Pb) and 90 % enriched 208Pb lead (208Pb(90 %)) in terms of increasing the neutron production while minimizing the total dose equivalent rate. The laboratory-derived optimal configuration for the (reflector/shield) assembly demonstrated that Pb significantly increased the neutron flux by approximately 58 % and reduced the total dose equivalent rate to 0.00443 mSv/h, thereby allowing for a workload of 4500 h within the annual permissible dose limit of 20 mSv. The MCNP simulations validated the potentials of Pb and confirmed the superiority of 208Pb(90 %) in terms of increasing neutron flux to about 26 % compared to that measured for Pb, while also minimizing the total dose equivalent rate to 0.0037 mSv/h. Thus, enabling a workload of approximately 5400 h, which is higher by about 20 % compared to that permitted by Pb based on measurements. The geometrical configuration and results promoted the use of the proposed TNI device primarily for prompt gamma neutron activation analysis (PGNAA).

The applicability of prompt-gamma activation analysis to obsidian provenance studies—The case of a strange find: a blade from Csongrád (South-East Hungary)

In the last twenty years, prompt-gamma activation analysis (PGAA) turned out to be successful in provenance research of obsidians, i.e. in the identification of possible raw material sources of various archaeological objects. Since the early 2000s, a significant database has been built at the Budapest Neutron Centre, which includes compositional data of about 500 geological and archaeological obsidian items, representing the major European and Mediterranean sources. Besides the straightforward cases of provenance studies, however, there are still a few difficult questions to answer. During the excavations of a unique grave at Csongrád (SE Hungary), a long transparent obsidian blade was found together with other objects. The grave was dated to 4370–4239 (1σ, 68.2%) cal. BC, belonging to the first wave of the ‘Pit Grave’ culture and having strong eastern contacts. Following the first energy dispersive X-ray spectroscopy (EDS) measurement in 1983, the blade was compared to the Carpathian 1 (Slovakian) type raw material. In 2019, PGAA and portable X-Ray Fluorescence analysis have been done on the same piece. Based on the first results, the Csongrád specimen, unlike other archaeological samples studied so far, was not possible to unambiguously associate with any of the known obsidian types. In order to clarify the provenance of the Csongrád blade, we have extended our database with more geological reference data from both sides of the Caucasus (i.e. from Armenia and Georgia), which are the closest outcrops to the main distribution region of the Pit Grave Culture in the NE part of the Black Sea. Until now, still no reassuring explanation is available concerning the provenance of the object with unusual composition.

Determination of Minor and Trace Elements in Breast Milk of Lactating Mothers in Early Lactation from Tehran, Iran Using Neutron Activation Analysis Method.

This study aimed to evaluate the concentrations of both essential and non-essential elements in the breast milk of lactating mothers from Tehran, Iran, during the colostrum period. Neutron activation analysis (NAA) was used to measure the element concentrations. Additionally, the study assessed how these element concentrations were influenced by maternal factors such as age and economic status. Breast milk samples were collected from 95 lactating mothers, aged 18 to 41, during the early lactation phase, specifically within the colostrum period (2-7 days postpartum). The colostrum milk samples were freeze-dried, powdered, and irradiated at the Tehran Research Reactor for neutron activation analysis (NAA). This method was used to measure the concentrations of essential elements-calcium (Ca), potassium (K), sodium (Na), chlorine (Cl), and iodine (I)-as well as non-essential elements-aluminum (Al), bromine (Br), and rubidium (Rb). Descriptive statistics, including mean, median, maximum, minimum, and standard deviation, were calculated for each element. Statistical analyses, such as Pearson's correlation, were performed to assess relationships between the concentrations of various elements. Additionally, t-tests and p-values were employed to evaluate differences in element levels across maternal age groups (17-34 years vs. 35-45 years) and economic status (high/middle vs. low). The mean concentrations of the elements in dry breast milk powder samples were: Al = 6.9mg/kg, Br = 11.9mg/kg, Ca = 2.757mg/g, Cl = 7.836mg/g, I = 1.22mg/kg, K = 5.853mg/g, Na = 4.932mg/g, and Rb = 3.69mg/kg. Significant correlations were found between element pairs, such as Na-Cl, Br-Cl, Na-Br, Rb-K, and I-Cl. Maternal age significantly influenced bromine concentrations, with older mothers showing 22% higher Br levels (p = 0.038), while calcium levels were 15% lower but not statistically significant (p = 0.20). Maternal economic status significantly impacted calcium and potassium concentrations, with higher levels observed in mothers from better economic conditions (p = 0.02 and p = 0.025, respectively). This study highlights the elemental composition of breast milk samples of lactating mothers in Tehran and shows that maternal factors, such as age and economic status, can significantly influence the concentrations of specific elements in breast milk.

Ore Formation and Mineralogy of the Alattu–Päkylä Gold Occurrence, Ladoga Karelia, Russia

The Alattu–Päkylä gold occurrence is located in the Northern Lake Ladoga area, in the Raaha-Ladoga suprasubduction zone, at the Karelian Craton (AR)—Svecofennian foldbelt (PR1) boundary. Its gold ore mineral associations are of two types of mineralization: (1) copper–molybdenum–porphyry with arsenopyrite and gold (intrusion-related) and (2) gold–arsenopyrite–sulfide in shear zones. Optical and scanning electron microscopy, X-ray fluorescence spectrometry, inductively coupled plasma mass spectrometry (ICP-MS), instrumental neutron activation analysis (INAA) and fire analysis with AAS finishing were used to study them. Type 1 was provoked by shallow-depth tonalite intrusion (~1.89 Ga) and type 2 by two stages of Svecofennian metamorphism (1.89–1.86 and 1.83–1.79 Ga) with the possible influence of the impactogenesis of the Janisjärvi astrobleme (age ~1 Ga). Intrusive and host rocks were subjected to shearing accompanied by the formation of ore-bearing metasomatic rocks of the propylite-beresite series (depending on substrate) and quartz–sericite, quartz and sericite–tourmaline veins and streaks. Ore mineralization is present as several consecutive mineral associations: pyritic–molybdenite with arsenopyrite and gold; gold–arsenopyrite; quartz–arsenopyrite with antimony sulfosalts of lead; gold–polysulfide with tetrahedrite –argentotetrahedrite series minerals and gold–antimony with Pb–Sb–S system minerals and native antimony. Arsenopyrite contains invisible (up to 234 ppm) and visible gold. Metamorphosed domains in arsenopyrite and rims with visible gold around it are usually enriched in As, indicating higher (up to >500 °C) temperatures of formations than original arsenopyrite with invisible gold (<500 °C). A paragenetic sequence associated with the deposition of invisible and visible gold established at the Alattu–Päkylä ore occurrence: pyrrhotite + unaltered arsenopyrite (with invisible gold) → altered arsenopyrite (As-enriched) + pyrite ± pyrrhotite + visible gold. Gold, associated with gudmundite, sphalerite and native antimony, seems to be due to cainotypic rhyodacitic porphyry cutting tonalite intrusion or with a retrograde stage in post-Svecofennian metamorphism. The isotopic composition of Pb and 238U/204Pb (9.4–9.75) for the feldspar of the tonalite intrusion and the pyrite of gold mineralization, εNd (−4 up to −5) for tonalites and ẟ34S values of −2.10–+4.99 for arsenopyrite, indicate the formation of gold occurrence provoked by Svecofennian magmatic and tectono-thermal processes with the involvement of matter from a mantle-lower crustal reservoir into magma formation and mineralization.

Microbial-induced structural changes in non-stoichiometric magnetite via radioanalytical methods

AbstractStructural alterations in non-stoichiometric magnetite induced by microorganisms are studied by nuclear methods. Magnetite samples were exposed to fungal strain known for its immobilization capabilities. Neutron activation analysis identified iron as the dominant element. The presence of maghemite in non-stoichiometric magnetite complicates the determination of iron sites by Mössbauer spectrometry. Mössbauer spectra recorded at 4.2 K display overlapping lines corresponding to tetrahedral and octahedral iron sites. Application of external magnetic field of 6 T improved the spectral resolution and revealed a presence of distinct sextets. Interaction with Aspergillus niger did not notably affect magnetite´s magnetic properties, indicating its stability.

Processing demands modulate the activities and functional connectivity patterns of the posterior (VWFA-1) and anterior (VWFA-2) VWFA

Previous studies have shown that the visual word form area (VWFA) has structural and intrinsic functional connectivity with both language and attention networks. Nevertheless, it is still unclear how the functional connectivity pattern of the VWFA is regulated by processing demands induced by experimental tasks, and whether processing demands differentially regulate the posterior (VWFA-1) and anterior (VWFA-2) subregions of the VWFA. To address these questions, the present study adopted two tasks varying in processing demands (i.e., verbal and non-verbal tasks), and used generalized psychophysiological interaction (gPPI) and dynamic causal modeling (DCM) analyses to explore the task-dependent functional connectivity patterns of the two subregions of the VWFA. Activation analysis revealed that the VWFA-2 showed higher activation for the verbal task than the non-verbal task, while there were no activation differences in the VWFA-1 after controlling for the stimulus driven effects. Functional and effective connectivity analyses revealed that, for both VWFA-1 and VWFA-2, the verbal task enhanced connections from VWFAs to the ventral language regions (e.g., the left orbital frontal cortex), while the non-verbal task enhanced connections from VWFAs to the dorsal visuospatial regions (e.g., the left intraparietal sulcus). Results of the present study indicate that processing demands induced by tasks modulate both the local activity and functional connectivity patterns of the VWFA, providing new insights for understanding its domain-general function.

Moss Technique Used to Monitor the Atmospheric Deposition of Trace Elements on the Territory of the Ryazan Region, Russia

Instrumental neutron activation analysis in combination with the method of moss biomonitoring for the assessment of atmospheric deposition of heavy metals and other elements was applied for the first time in the Ryazan Region in Russia. Concentrations of 42 macro, micro and trace elements in 63 moss samples (Hylocomium splendens and Pleurozium schreberi, as well as Hypnum cupressiforme, Sciuro-hypnum sp., Plagiothecium sp. and Ptilium sp.), collected on a relatively uniform grid in the study area, were determined. On the basis of analytical results using GIS technologies, maps of the spatial distribution of heavy metals and other toxic elements were constructed for the Ryazan region. The method of multivariate statistical analysis (factor analysis) was used to identify the main sources of pollution—large industrial facilities located in this region. The obtained results were included in the Atlas of Atmospheric Deposition of Heavy Metals, which is published by the UN Commission on Long-Range Transboundary Air Pollution (UNECE ICP Vegetation).

Radionuclides' Dispersion from Coal-Fired Brick Kilns: Geo-Environmental Processes, Potential Risks and Management.

In order to investigate the distributions and possible dispersion mechanism(s) of naturally occurring radioactive materials (NORMs: 226Ra, 232Th, and 40K) from coal-based brick kilns, a systematic set (n = 60) of coal, ash, surface-soil, and subsurface soil samples were analyzed. High-quality analytical data of U, Th and K obtained from HPGe detector and TRIGA Mark-II research reactor-based neutron activation analysis were converted to the corresponding radioactivities. Average (n = 10) radioactivities of 226Ra, 232Th, and 40K in coal samples were 15.6, 16.7, and 145.5Bq.kg-1, respectively, where only 40K surpassed the corresponding global mean value. Average (n = 10) radioactivities of 226Ra, 232Th, and 40K in ash samples were 62.7, 88.5, and 521Bq.kg-1, respectively, where only 226Ra was within the established limit. In soil samples, average (n = 40) activities of 226Ra, 232Th, and 40K were 62.7, 95.1, and 641Bq.kg-1, respectively, which have surpassed the corresponding worldwide mean values. The observed differences in activity levels between soil samples collected near and far from the kilns, as well as between topsoil and subsoil samples, suggest the presence of distinct transport mechanisms for NORMs within the pedosphere. Dispersions of NORMs from the brick kilns to the ambient pedosphere are largely governed by aerodynamic convection and hydrodynamic leaching. These mechanisms are also influenced by geochemical mobility and relative solubility of NORMs, as well as factors such as rainfall patterns and wind-flow direction. Radiological indices invoke long-term carcinogenic-risks, whereas aerodynamic convection of finer particles (coal fly ash) from chimneys can cause significant health hazards to the nearby dwellers. Scientific processes as well as public awareness are essential to mitigate the risks.

Application of LIBS and LA-ICP-MS analysis to Hellenistic tableware

Greek and Hellenistic painted vases have attracted scholars of classical archaeology for centuries, either because of the artistic value of the painted decorations or because of the depicted scenes of ancient everyday life. Due to the application of thin slips on the surface, different methods are used for the chemical characterisation of the slips and the body of the same vase. The slips are commonly analysed by X-ray microanalysis, offering point-by-point analysis of mostly major elements, while different tools, such as X-ray fluorescence (XRF), inductively coupled mass spectrometry (ICP-MS), and instrumental neutron activation analysis (INAA), are used for bulk body analysis. This can cause difficulties in comparing the bulk composition and thus in determining the similarities and differences in the preparation process of the clay paste for the slips and bodies of an object. Given the artistic value of these objects, museum curators tend to be reluctant to provide samples for invasive characterisation. Micro-destructive laser ablation methods offer a robust solution to addressing both the relationships between ceramic body and surface treatment chemistry and destructive sampling. To provide a proof-of concept, we analysed slips and bodies of a small sample of Hellenistic fine wares from the Greek colony of Issa in modern Croatia with Laser-Induced Breakdown Spectroscopy (LIBS) and Laser ablation-ICP-MS. Results show remarkable diversity in the use of clay types and processing techniques.

Sensitivity improvement of a deuterium-deuterium neutron generator based in vivo neutron activation analysis (IVNAA) system.

Our lab has been developing a deuterium-deuterium (DD) neutron generator-based neutron activation analysis (NAA) system to quantify metals and elements in the human body in vivo. The system has been used to quantify metals such as manganese, aluminum, sodium in bones of a living human. The technology provides a useful way to assess metal exposure and to estimate elemental deposition, storage and biokinetics. It has great potential to be applied in the occupational and environmental health fields to study the association of metal exposure and various health outcomes, as well as in the nutrition field to study the intake of essential elements and human health. However, the relatively low sensitivity of the system has greatly limited its applications. Neutron moderation plays an important role in designing an IVNAA facility, as it affects thermal neutron flux in irradiation cave and radiation exposure to the human subject. This study aims to develop a novel thermal neutron enhancement method to improve the sensitivity of the in vivo neutron activation analysis (IVNAA) system for elemental measurement but still maintain radiation dose. Utilizing a compact DD neutron source, we propose a new and practical moderator design that combines high density polyethylene with heavy water to enhance thermal neutrons by reducing thermal neutron absorption. All material dimensions are calculated by PHITS, a general-purpose Monte Carlo simulation program. The improvement of the new design predicted by the Monte Carlo simulation for the quantification of one of the elements, manganese was verified by experimental irradiation of manganese-doped bone equivalent phantoms. For the same radiation dose, a 67.9% thermal neutron flux enhancement is reached. With only 4.2% increase of radiation dose, the simulated thermal neutron flux and activation can be further increased by 84.2%. A 100% thermal neutron enhancement ratio is also achievable with a 20% dose increase. The experimental results clearly show higher manganese activation gamma ray counts for each specific phantom, with a significantly reduced minimum detection limit. Additionally, the photon dose was suppressed. The thermal neutron enhancement method can increase the number of useful neutrons significantly but maintain the radiation dose. This greatly decreased the detection limit of the system for elemental quantification at an acceptable dose, which will broadly expand the application of the technology in research and clinical use. The method can also be applied to other neutron medical applications, including neutron imaging and radiotherapy.

Experimental gamma coincidence spectra recorded in prompt gamma neutron activation analysis

Experimental gamma coincidence spectra recorded in prompt gamma neutron activation analysis

Mineral Composition of Fifteen Species of Asteraceae Family Growing in the Republic of Moldova Using Neutron Activation Analysis

Members of Asteraceae family have properties enabling their application for medical purposes. The major- and microelement content in 15 medicinal species of the Asteraceae family growing in the National Botanical Garden, Republic of Moldova was determined by instrumental neutron activation analysis. A total of 31 chemical elements, Na, Mg, Al, Cl, K, Ca, Sc, V, Cr, Mn, Fe, Co, Ni, Zn, As, Se, Br, Rb, Sr, Mo, Sb, Cs, Ba, La, Ce, Sm, Tb, Hf, Ta, Th and U, were identified in herbal samples. Potassium was found to be the most abundant major element; its content in plants ranged from 20,700 mg/kg in Artemisia lerchiana to 58,000 mg/kg in Matricaria recutita. The content of other major elements existed in the following ranges: Ca from 4700 to 14,200 mg/kg and Mg from 1710 to 3870 mg/kg. The maximum content of Mg, K and Ca in analyzed plants was higher compared to data presented in the literature. Among essential microelements the most abundant were Fe (83–910 mg/kg), Mn (23–150 mg/kg) and Zn (27–76 mg/kg). The daily intake of metal and the health risk index for selected elements (As, Ni, Sb, V, Mn, Cr, Co, U, Sr, Al, Fe, and Zn) were calculated. Health risk indexes were obtained for V and Co in Calendula officinalis, Tanacetum balsamita, Achillea clypeolata, Artemisia balchanorum, Artemisia lerchiana, Helichrysum arenarium, and Matricaria recutita. The principal component analysis showed three associations of elements, which can be defined as physiological, geological and anthropogenic sources of elements.

Development of a high-yield compact D-D neutron generator

An improved high-yield compact D-D neutron generator has been developed for active neutron non-destructive interrogation at Lanzhou University in China. The generator has been meticulously designed based on the magnetic field distribution of the duoplasmatron ion source, the electric field distribution of the beam extraction acceleration system, beam transport, and target cooling system. The performance characteristics of the generator were measured under different deuterium beam energies and beam intensities. The experimental results indicated that the D-D neutron yield reached 1 × 109 n/s with the deuterium beam parameter of 210 keV/6.0 mA. The operational stability of the generator was assessed for 150 min, and the test results show that the generator has better stability in operation. This generator has potential applications in neutron radiography, active interrogation of special nuclear materials, and neutron activation analysis.

Explore the expression of mitochondria-related genes to construct prognostic risk model for ovarian cancer and validate it, so as to provide optimized treatment for ovarian cancer.

The use of gene development data from public database has become a new starting point to explore mitochondrial related gene expression and construct a prognostic prediction model of ovarian cancer. Data were obtained from the TCGA and ICGC databases, and the intersection with mitochondrial genes was used to obtain the differentially expressed genes. q-PCR, Cox proportional risk regression, minimal absolute contraction and selection operator regression analysis were performed to construct the prognostic risk model, and ROC curve was used to evaluate the model for centralized verification. The association between risk scores and clinical features, tumor mutation load, immune cell infiltration, macrophage activation analysis, immunotherapy, and chemosensitivity was further evaluated. A prognostic risk score model for ovarian cancer patients was constructed based on 12 differentially expressed genes. The score was highly correlated with ovarian cancer macrophage infiltration and was a good predictor of the response to immunotherapy. M1 and M2 macrophages in the ovarian tissue in the OV group were significantly activated, providing a reference for the study of the polarity change of tumor-related macrophages for the prognosis and treatment of ovarian cancer. In terms of drug sensitivity, the high-risk group was more sensitive to vinblastine, Acetalax, VX-11e, and PD-0325901, while the low-risk group was more sensitive to Sabutoclax, SB-505124, cisplatin, and erlotinib. The prognostic risk model of ovarian cancer associated to mitochondrial genes built on the basis of public database better evaluated the prognosis of ovarian cancer patients and guided individual treatment.

Determination of fluorine in reference materials by chopped-beam cold neutron prompt gamma-ray activation analysis (CB-CNPGAA)

AbstractA method is described for determination of fluorine in reference materials by chopped beam cold neutron prompt gamma-ray activation analysis (CB-CNPGAA). The chopper cycle is set to 11 s on/off to match the t1/2 of 20F. To reduce systematic error, the F mass is measured as the ratio to a comparator element, either Ca or Cl. The F mass fraction is then calculated by multiplying this ratio by the mass fraction of the comparator element. The method is used to measure F in SRMs 1486 Bone Meal, 1400 Bone Ash, 1566a Oyster Tissue, and 2695 Fluorine in Vegetation.

Application of instrumental neutron activation analysis (INAA) to ceramics and clay sources for the late period (c. 950 years BP) in the Southern Argentine Puna: Interweaving sources, objects and practices

AbstractThe study of the raw materials used in the production of ceramic objects involves studying the materiality of the objects themselves while providing insights into the factors that guided the choice of a particular resource over another. Simultaneously, this article focuses on the social contexts in which these objects were produced. We present the results obtained through an instrumental neutron activation analysis (INAA) of ceramics from the Corral Alto site (Antofagasta de la Sierra, Catamarca) of Argentina. The results suggest that most ceramic pieces were locally manufactured, contrasting with earlier periods and coinciding with the emergence of new ways of dwelling in the Southern Argentine Puna.