Particle cross-stream migration in electrohydrodynamic microfluidic systems exhibits intriguing behaviors, which makes it interesting when viewed from a fundamental perspective and promising for nanoparticle focusing and separation applications. So far, particle behavior in such systems has been explained with the slip-induced lift force model (Saffman model), which predicts particle central or side focusing based on the direction of electric field and fluid flow. However, in our previous work, we observed particle migration patterns that did not adhere to the prediction of the Saffman model. In this work, we further studied this novel particle lateral migration behavior, which we termed the “anti-Saffman” behavior. We experimentally investigated how changing the conductivity of the suspending medium influences particle behavior and quantitatively measured the net lateral force experienced by the particles. Then, we compared this net force with the prediction of the relevant lift force models in the literature. We concluded that the anti-Saffman behavior is positively correlated with medium conductivity and shear rate (∝γ̇2). Furthermore, the comparison with the existing force models revealed that none of them can predict the experimentally observed particle lift. The net lift predicted by hydrodynamic lift models indicated that the underlying mechanism behind our experiments also potentially has a hydrodynamic origin. We believe this phenomenon offers the possibility of manipulating and separating nanoparticles suspended in standard aqueous electrolyte solutions, which makes it applicable to various biological samples.
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