Relevance. Geological prospecting carried out recently throughout the Lena-Tunguska oil-and-gas province makes studies of carbonates of the Osinskaya subformation particularly relevant, as they make it possible to establish the patterns of hydrocarbon trap formation. Aim. To present the results of the lithological and facial, biostratigraphic and petrophysical studies of the Osinskaya subformation in the southern and central part of the Nepa-Botuoba anteclise. Objects. Carbonate rocks of the middle Osinskaya subformation of the Usolskiy Formation of the Lower Cambrian Tommotian Stage, studied from well cores. Methods. GIS complex, detailed lithological and sedimentological description of the core, sequential stratigraphic analysis, petrographic analysis of 300 samples and examination of paleontological remains taken from the core; analysis. Results. Based on these studies, three sedimentation zones were identified, traced, and described for the first time: deep-water, marginal, and shallow-water. The deep-water zone is characterized by uncompensated sedimentation, the presence of single reefs, and confinement to depressions and troughs. It was flanked by an edge zone of carbonate platform associated with elevated thicknesses of the subformation and distribution of reefs over the area. It was followed by a shallow-water zone, represented by medium and reduced thicknesses of the Osinskaya subformation, its sediments are composed of carbonate grained and clayey rocks with small single reefs. The localized marginal zone of the extended carbonate platform agrees with the previously identified Chambinsko-Altybsko-Mirninskaya and Verkhnetokhomsko-Katsko-Pilyudinskaya zones of distribution of reef-like organogenic structures. On the basis of the studied wells, the structure of three formations according to the lithologic-facial profile was described. The paper introduces the authors’ variant of conducting the boundaries of the members. It was revealed that in the deep-water zone the prospects of searching for reservoir rocks are associated with single carbonate structures, in the marginal zone with area bioherms, and in the shallow-water zone with detrital deposits and shallow bioherms. The paleontological finds are shown to be confined to the selected sedimentation zones and beds. It was determined that algae diagnosed in the boudstones (autochthonous) were involved in the formation of the second and third strata, the edge zone of the carbonate platform. Archaeocyaths were diagnosed in detrital carbonate rocks of the second pack (allochthonous) in well no. 7 and autochthonous in boudstones of well no. 2X. Cribriciates were diagnosed in the boudstones of the third member (autochthonous). Archaeocyaths, cribriciates, namacalatusses, and calcareous algae studied from the cores of wells West Yaractinian no. 45, 361, Bolshetyrskaya no. 7, 3X, 2X, 4X, 5X, 6X were major edificators of Early Cambrian organogenic structures (biostromes, bioherms, bioherms massifs, reef formations).