Neoplastic Lesions Of Uterine Cervix Research Articles

Immunohistochemical expression of P16 and Ki-67 in uterine cervical neoplasms

To study the expression of Ki-67 and p16 in neoplastic lesions of uterine cervix and to evaluate the prognostic significance of tumour differentiation, histological type, stage and grade, depth of tumour invasion and lymphovascular invasion in women with neoplasia of uterine cervix. This study was performed on 50 biopsies and surgically resected specimens of uterine cervix, which were fixed in 10% formalin, processed, paraffin embedded and cut into 3-5 microns thickness, stained with hematoxylin and eosin stains and immunohistochemical staining by p16 and Ki-67 antibodies was performed and studied. Majority of cases of invasive carcinoma cervix were large cell non-keratinizing squamous cell carcinoma, 58 cases (48.2%). Majority of cases of invasive carcinoma cervix were seen in stage 2B, 40 (40.8%) cases followed by stage 3B, 24 (24.4%) cases. Out of 18 cases of large cell non-keratinizing squamous cell carcinoma, 12 (66.7%) showed 3+ positivity for Ki-67. 7 (53.8%) cases of stage 2A showed 3+ positivity, 3 (23.1%) cases each showed 2+ positivity for p16. 7 (36.8%) cases of moderately differentiated SCC showed 3+ positivity, 4 (21.1%) showed 2+ positivity and 5 (26.3%) showed 1+ positivity of Ki-67. Out of 10 stable patients, 4 (40.0%) showed negative p16 immunoexpression and all the 3 cases (100%) with recurrence showed 2+ positivity and 2 (100%) patients with metastasis showed 3+ positivity. Out of 10 stable patients, 6 (60%) showed negative Ki-67 immunoexpression and all the 3 (100%) cases with recurrence showed 3+ positivity and one each (50.0%) patient with metastasis showed 2+ positivity and 3+ positivity. p16 and Ki-67 immunomarkers are useful as a diagnostic and prognostic tool in cases with recurrence and metastasis, helping in early detection of disease progression.

Immunohistochemistry study for HER2/neu expression in pre-neoplastic and neoplastic lesions of uterine cervix

Immunohistochemistry study for HER2/neu expression in pre-neoplastic and neoplastic lesions of uterine cervix

Histopathological evaluation of Non-Neoplastic and Neoplastic Lesions of Uterine Cervix at tertiary care centre

Background: Uterine cervix is common specimen from gynecological department with non-neoplastic and neoplastic lesions. Most cervical cancers can be detected at pre-invasive state with an adequate screening and treated appropriately thus preventing overt progression to full blown cancer and hence decreasing morbidity and mortality. Carcinoma of cervix is a preventable tumor and if good effort given in detecting preinvasive lesion one can give definite treatment at earliest. Objectives: To evaluate the histopathological diagnosis of a biopsy of cervix in women with unhealthy cervix. Also, to study the agerelated, incidence and the incidence of various nonneoplastic and neoplastic lesions of the cervix. Methodology: It is a cross sectional study. Institutional Ethics committee permission was taken prior to start of the study. Pertinent clinical history like age, significant family and personal history, history of other diseases was taken. Cervical biopsy specimens collected from histopathology section and grading of cervical lesions was done based on the proportion of stained cells and the intensity of staining. Statistical analysis was done. The data were tabulated and frequencies and percentages were calculated. Observations: The common age group was 41-50 years (37.50%) followed by 31-40 years of age (26.25%). The symptoms with which patients presented were vaginal bleeding (52.50%) followed by white discharge per vagina (16.25%) and menorrhagia (12.50%). The premalignant lesions were seen in 12 cases (15.0%) and malignant lesions in 68 cases (85.0%). Among them 64 malignant tumours were epithelial in origin. As per microscopic examinations squamous cell carcinoma was diagnosed in 61 cases (93.75%), followed by 2 cases (3.13%) of adenocarcinoma, and 1 case (1.58%) of adenosquamous carcinoma and 1 case (1.54%) of rhabdomyosarcoma. Conclusion: Cervical cancer continues to be the most common cancer of females in developing countries. One of the most significant advances in the management of cervical neoplasms has been the realization that cervical intraepithelial lesions behave as progressive stages of a biologic continuum towards the development of invasive cancer.

Significance of mast cells in non-neoplastic and neoplastic lesions of uterine cervix

Background: Mast cells are involved in multiple biological events. The significance of mast cells in non-neoplastic and neoplastic lesions of cervix has been studied with conflicting results. Its presence in tumor has been described as evidence of host immunologic antitumor response, angiogenesis and tumor invasion. Aims/Objectives: To study mast cell density in various cervical lesions using Toluidine blue stain; To compare the sections studied with conventional Toluidine blue staining and Toluidine blue staining by reducing the pH. Methodology: Cervical biopsy/hysterectomy specimens from archives of Department of Pathology were considered for the study. The sections were studied for histomorphology and mast cell density. The mast cell density was assessed by Toluidine blue staining by conventional method and another method by reducing pH using weak HCL. The stained slides were reviewed for mast cell density under 10 high power field and statistically analysed. Results: Total of 100 cases were studied. Normal cervix 7 cases with mean age of 44.29 and mast cell density (mean) of 45.43. Chronic cervicitis and polypoidal Endocervicitis were 26 and 28 cases, mean age of 45.38 years and 39.14 years and mean mast cell count of 48.38 and 66.96 respectively. Intraepithelial lesions and malignancy were 23 and 16 cases, mean age of 43.56 years and 52.26 years and mean mast cell of 34.47and 34.6 respectively. Maximum number of mast cells was seen in polypoidal Endocervicitis and least number in Squamous cell carcinoma of cervix. Conclusion: The role of mast cell differs in inflammatory and neoplastic lesions of cervix. Mast cells has active role in inflammatory lesions.

G801A Polymorphism of Human Stromal Cell–Derived Factor 1 Gene Raises No Susceptibility to Neoplastic Lesions of Uterine Cervix

This study aimed to investigate the association of stromal cell-derived factor 1 (SDF-1) gene polymorphisms with the neoplastic lesions of uterine cervix in Mid-Taiwan women. Four hundred ninety-eight blood samples were collected from 161 patients with neoplasia of uterine cervix, including 76 cancer patients, 61 patients with high-grade dysplasia, and 24 with low-grade dysplasia, and 337 healthy controls who lived in Mid-Taiwan. Polymorphism of the SDF-1 gene was examined using polymerase chain reaction-restriction fragment length polymorphism. For SDF-1 gene polymorphisms, the wild-type homozygous alleles (G/G) yielded 100- and 193-bp products, the heterozygous alleles (G/A) yielded 100-, 193- and 293-bp products, whereas the mutated-type homozygous alleles (A/A) yielded a 293-bp product. We found no significant difference in genotypes or alleles distribution of SDF-1 polymorphisms between patients with cervical neoplasia and healthy women (P = 0.530). Compared with the homozygous GG subgroup, GA and AA subgroups do not increase the risk of cervical neoplasia. Although the expression of SDF-1 was reported to be significantly increased in cervical carcinogenesis in previous studies, our results, however, show that SDF-1 gene polymorphism could not be considered as a factor related to an increased susceptibility to cervical neoplasia.

Polymorphisms of Human Nonmetastatic Clone 23 Type 1 Gene and Neoplastic Lesions of Uterine Cervix

Single-nucleotide polymorphisms (SNP) in promoter of human nonmetastatic clone 23 type 1 (nm23-H1) may affect their binding with transcription factors and affect promoter activity as well as gene transcription. Therefore, we investigated the impact of the nm23-H1 gene polymorphisms on the neoplastic lesions of uterine cervix in mid-Taiwan women (women who live in the central area of Taiwan). We expected that women with different genotypes in nm23-H1 polymorphisms, such as rs34214448, rs16949649, or rs2302254, may have different incidences of cervical neoplasia. In total, 366 blood samples were collected from 244 healthy women and 122 patients with cervical neoplasia to analyze 3 nm23-H1 gene single-nucleotide polymorphisms (rs34214448, rs16949649, and rs2302254). The heterozygous genotypes, TG in rs34214448 or TC in rs16949649, were differentially distributed between patients with cervical neoplasia and normal women (Hommel adjusted P = .0440 and .0435, respectively) as compared to their homozygotes. Moreover, compared to those with wild-type homozygotes and heterozygotes, women with variant homozygotes TT in rs34214448 or CC in rs16949649 exert different distributions between patients with cervical neoplasia and normal women (P = .058 and .058). Interestingly, we found the genotype distribution of rs34214448 has significant association with that of rs16949649 with high consistency. Mid-Taiwan women with the polymorphic heterozygotes TG in rs34214448 or TC in rs16949649 of human nonmetastatic clone 23 type 1 promoter have the tendency to develop cervical neoplasia while compared to their homozygous counterparts. However, women with variant homozygotes TT in rs34214448 or CC in rs16949649 exhibit less tendency as compared to those with wild-type homozygotes and heterozygotes.