Background: Lemongrass are found in Indonesia thus it lemongrass can be used as an alternative in healinginflammation by conducting a preliminary study in the form of BHK-21 fibroblast cell viability test after 24hours of extract lemongrass.Objective: To determine the viability of extract lemongrass against BHK-21 fibroblast cell cultures. Method:BHK-21 fibroblast cells were taken from neonatal hamsters’ kidneys and planted in 96 well plates. Extractslemongrass with concentrations of 100%, 50%, 25%, 12.5%, 6.25%, 3.13% and 1.56% were included ineach well prepared and incubated for 24 hours. MTT Assay was performed to see the viability of extractlemongrass on fibroblast cells.Results: Extract lemongrass viable against BHK-21 fibroblast cells at a concentration of 100%. There is adecrease in viability at concentrations of 50% to 1.56%.Conclusion: The 100% concentration showed the highest viability of BHK-21 fibroblast cells and extractlemongrass had potential in wound healing.