Negligible Correlations Research Articles

Criterion Validity of Screening Tools and Field-Based Tests for Health-Related Physical Fitness in Inflammatory Bowel Disease.

Monitoring health-related physical fitness (HRPF) may benefit proactive Inflammatory Bowel Disease (IBD) management. However, knowledge regarding HRPF in patients with IBD is limited and gold standard tests are impractical for widespread use, necessitating simpler methods. This study evaluated the criterion validity of screening tools and field-based tests compared to gold standard tests for HRPF in patients with IBD. Adult patients with IBD completed screening tools, field-based tests, and gold standard tests for HRPF. Criterion validity was examined through (intraclass) correlation coefficients and Bland-Altman plots. Predictive capacity of the screening tools was examined with receiver operating curve analysis. Among 53 included patients, screening tools demonstrated poor-to-moderate validity compared to the cardiopulmonary exercise test (CPET) for cardiorespiratory fitness. Very strong correlations were found for four-site skinfold thickness and multi-frequency bioimpedance analysis (BIA) with deuterium oxide dilution for body fat percentage (ICC = 0.90, ICC = 0.93), and between the steep ramp test and CPET (r = 0.95) for cardiorespiratory fitness. The steep ramp test also correlated strongly with isokinetic quadriceps (r > 0.75) and hamstring (r > 0.74) strength. Hand-held dynamometry and the sit-to-stand test showed strong correlations with hamstring strength (r > 0.80, r > 0.76). Negligible correlations were found for field-based tests compared to isokinetic quadriceps and hamstring endurance. Four-site skinfold thickness and BIA showed good agreement with the gold standard for body fat measurement. The steep ramp test demonstrated strong correlations with the gold standard tests for cardiorespiratory fitness and quadriceps and hamstring strength, while hand-held dynamometry and the sit-to-stand test showed strong correlations with hamstring strength.

Diffusion Tensor Imaging of Rat Rotator Cuff Muscle with Histopathological Correlation: An Exploratory Study.

Diffusion tensor imaging (DTI) is a magnetic resonance imaging (MRI) technique that can be used to assess microstructural features of skeletal muscle that are related to tissue function. Although widely used, direct correlations between DTI derived metrics such as fractional anisotropy and spatially matched tissue microstructure assessed with histology have not been performed. This study investigated the relationship between scalar-based DTI measurements and histologically derived muscle microstructural measurements in rat rotator cuff muscles. Despite meticulous co-localization of MRI and histology data, negligible correlations were found between DTI metrics and histological measurements including muscle fiber diameter, cross-sectional area, and surface-to-volume ratio. These findings highlight the challenges in validating DTI with histology due to requirements in anatomical co-localization, necessity of high-quality histology, and consideration of diffusion measurement scales. Our findings underscore the need for further research with optimized imaging parameters to enhance our knowledge regarding the sensitivity of DTI to important features of muscle microstructure.

Multitrait Analysis to Decipher the Intertwined Genetic Architecture of Neuroanatomical Phenotypes and Psychiatric Disorders

BackgroundThere is increasing evidence of shared genetic factors between psychiatric disorders and brain magnetic resonance imaging (MRI) phenotypes. However, deciphering the joint genetic architecture of these outcomes has proven to be challenging, and new approaches are needed to infer the genetic structures that may underlie those phenotypes. Multivariate analyses are a meaningful approach to reveal links between MRI phenotypes and psychiatric disorders missed by univariate approaches. MethodsFirst, we conducted univariate and multivariate genome-wide association studies for 9 MRI-derived brain volume phenotypes in 20,000 UK Biobank participants. Next, we performed various complementary enrichment analyses to assess whether and how univariate and multitrait approaches could distinguish disorder-associated and non–disorder-associated variants from 6 psychiatric disorders: bipolar disorder, attention-deficit/hyperactivity disorder, autism, schizophrenia (SCZ), obsessive-compulsive disorder, and major depressive disorder. Finally, we conducted a clustering analysis of top associated variants based on their MRI multitrait association using an optimized k-medoids approach. ResultsA univariate MRI genome-wide association study revealed only negligible genetic correlations with psychiatric disorders, while a multitrait genome-wide association study identified multiple new associations and showed significant enrichment for variants related to both attention-deficit/hyperactivity disorder and SCZ. Clustering analyses also detected 2 clusters that showed not only enrichment for association with attention-deficit/hyperactivity disorder and SCZ but also a consistent direction of effects. Functional annotation analyses of those clusters pointed to multiple potential mechanisms, suggesting a role of neurotrophin pathways in particular in both MRI and SCZ. ConclusionsOur results show that multitrait association signature can be used to infer genetically driven latent MRI variables associated with psychiatric disorders, thereby opening paths for future biomarker development.

Cooper quartets in interacting hybrid superconducting systems

Cooper quartets represent exotic fermion aggregates describing correlated matter at the basis of charge-4e superconductivity and offer a platform for studying four-body interactions, of interest for topologically protected quantum computing, nuclear matter simulations, and more general strongly correlated matter. Focusing on solid-state systems, we show how to quantum design Cooper quartets in a double-dot system coupled to ordinary superconducting leads through the introduction of an attractive interdot interaction. A fundamentally novel, maximally correlated double-dot ground state, in the form of a superposition of vacuum |0〉 and four-electron state |4e〉, emerges as a narrow resonance in a many-body quartet correlator that is accompanied by negligible pair correlations and features a rich phenomenology. The system represents an instance of correlated Andreev matter and the results open the way to the exploration of interaction effects in hybrid superconducting devices, and the study of novel correlated states of matter with ingredients available in a quantum solid-state laboratory. Published by the American Physical Society 2024

False Beliefs about Mathematics in Spanish Pre-Service Teachers and Relations with Math Anxiety.

Teachers may hold beliefs about how students learn mathematics and about the subject itself. However, some of these beliefs, often referred to as "math myths", may be oversimplified ideas that appear valid but lack solid scientific evidence. This study was aimed to investigate the prevalence of math myths among Spanish pre-service teachers and compare it with the degree of support for these myths reported in previous studies; investigate the possible underlying structure of a set of false beliefs about math; and determine the relationships of certain math misconceptions with math anxiety. A total of 212 Spanish pre-service teachers were administered questionnaires about math myths and about general and math anxiety. The results showed prevalence patterns of each math misconception similar to those in previous studies. False beliefs about math showed weak or negligible correlations with each other and did not exhibit a discernible underlying structure. Furthermore, math anxiety was related to the belief that some individuals are perceived to possess a "math mind" while others do not. Our results suggest diverse origins for the development of different math myths, rather than the myths being the consequence of a single underlying factor. Finally, the endorsement of certain beliefs about who can do mathematics may contribute to negative emotions towards mathematics.

Little associations exist between the three commonly used functional screening tests in collegiate athletes

Although an abundant number of studies have investigated the predictability of the commonly used functional screening tests and despite their popularity and applicability, the relationships between these tests have rarely been studied and have not been well established. This study aimed to examine the potential association between the Functional Movement Screen (FMS), Y Balance Test (YBT), and Landing Error Scoring System (LESS). Six hundred twenty-seven Iranian collegiate athletes (347 males, age = 22.63 ± 4.07, weight = 75.98 ± 13.79, height = 181.99 ± 10.15, BMI = 22.84 ± 3.16; and 280 females, age = 22.22 ± 3.37, weight = 60.63 ± 9.58, height = 166.55 ± 6.49, BMI = 21.81 ± 2.84) participated in this study. Following a 5-min warm-up, each participant underwent a standardized screening battery including the FMS, YBT, and LESS, and the scores were recorded and live coded for the statistical analysis, except for the LESS. The LESS tests were video recorded and scored by one expert examiner using an open-source 2D video analysis software (Kinovea- version 0.9.5), afterwards. The Spearman correlation was utilized as a measure for the correlation, and the Mann‒Whitney U test with a significance level of 0.05 was used to check the differences between male and female athletes. The statistical analysis was performed with RStudio 2023.03.0 using R 4.3.1. A small correlation (0.364) was observed between the FMS composite score and the YBT in male athletes. All other pairwise correlations were negligible among male and female athletes, ranging from − 0.096 to 0.294. Reducing the FMS to the component scores targeting the lower extremities did not alter the correlation to the other screening scores. The median FMS composite score in female athletes was significantly higher than that in males (p < 0.001). Negligible correlations exist between the FMS, LESS, and YBT; they do not measure the same values and therefore are irreplaceable with one another. A combination of these tests as a standardized screening battery may potentially better identify injury-predisposed athletes than the application of each test as a stand-alone screening test. Females outperformed males in the FMS test significantly, so sex must be considered a key variable in the FMS studies. Males had slightly higher LESS scores (median difference = 0.5) than females, but this difference is not clinically meaningful. Future research should continue to explore the relationships between various functional screening tests and identify the most effective combinations for comprehensive assessment in different populations and sports disciplines.

Correlating maternal and cord-blood inflammatory markers and BDNF with human fetal brain activity recorded by magnetoencephalography: An exploratory study

BackgroundDuring gestation, the brain development of the fetus is affected by many biological markers, where inflammatory processes and neurotrophic factors have been of particular interest in the past decade. AimThis exploratory study is the first attempt to explore the relationships between biomarker levels in maternal and cord-blood samples and human fetal brain activity measured with non-invasive fetal magnetoencephalography (fMEG). MethodTwenty-three women were enrolled in this study for collection of maternal serum and fMEG tracings immediately prior to their scheduled cesarean delivery. Twelve of these women had a preexisting diabetic condition. At the time of delivery, umbilical cord blood was also collected. Biomarker levels from both maternal and cord blood were measured and subsequently analyzed for correlations with fetal brain activity in four frequency bands extracted from fMEG power spectral densities. ResultsRelative power in the delta, alpha, and beta frequency bands exhibited moderate-sized correlations with maternal BDNF and cord-blood CRP levels before and after adjusting for confounding diabetic status. These correlations were negative for the delta band, and positive for the alpha and beta bands. Maternal CRP and cord-blood BDNF and IL-6 exhibited negligible correlations with relative power in all four bands. Diabetes did not appear to be a strong confounding factor affecting the studied biomarkers. ConclusionsMaternal BDNF levels and cord-blood CRP levels appear to have a direct correlation to fetal brain activity. Our findings indicate the potential use of these biomarkers in conjunction with fetal brain electrophysiology to track fetal neurodevelopment.

Investigation of intersegmental coordination patterns in human walking

BackgroundIntersegmental coordination between thigh, shank, and foot plays a crucial role in human gait, facilitating stable and efficient human walking. Limb elevation angles during the gait cycle form a planar manifold describes the by the planar covariation law, a recognized fundamental aspect of human locomotion. Research questionHow does the walking speed, age, BMI, and height, affect the size and orientation of the intersegmental coordination manifold and covariation plane? MethodsThis study introduces novel metrics for quantifying intersegmental coordination, including the mean radius of the manifold, rotation of the manifold about the origin, and the orientation of the plane with respect to the coordinate planes. A statistical investigation is conducted on a publicly available human walking dataset for subjects aged 19–67 years, walking at speeds between 0.18 and 2.3 m s−1 to determine correlations of the proposed quantities. We used two sample t-test and ANOVA to find statistical significance of changes in the metrics with respect to gender and walking speed, respectively. Regression analysis was used to establish relationships between the introduced metrics and walking speed. ResultsHigh correlations are observed between walking speed and the computed metrics, highlighting the sensitivity of these metrics to gait characteristics. Conversely, negligible correlations are found for demographic parameters like age, body mass index (BMI), and height. Male and female groups exhibit no practically significant differences in any of the considered metrics. Additionally, metrics tend to increase in magnitude as walking speed increases. SignificanceThis study contributes numerical metrics to characterize ISC of lower limbs with respect to walking speed along with regression models to estimate these metrics and related kinematic quantities. These findings hold significance for enhancing clinical gait analysis, generating optimal walking trajectories for assistive devices, prosthetics, or rehabilitation, aiming to replicate natural gaits and improve the functionality of biomechanical devices.

A non-invasive method to monitor respiratory muscle effort during mechanical ventilation.

This study introduces a method to non-invasively and automatically quantify respiratory muscle effort (Pmus) during mechanical ventilation (MV). The methodology hinges on numerically solving the respiratory system's equation of motion, utilizing measurements of airway pressure (Paw) and airflow (Faw). To evaluate the technique's effectiveness, Pmus was correlated with expected physiological responses. In volume-control (VC) mode, where tidal volume (VT) is pre-determined, Pmus is expected to be linked to Paw fluctuations. In contrast, during pressure-control (PC) mode, where Paw is held constant, Pmus should correlate with VT variations. The study utilized data from 250 patients on invasive MV. The data included detailed recordings of Paw and Faw, sampled at 31.25Hz and saved in 131.1-second epochs, each covering 34 to 41 breaths. The algorithm identified 51,268 epochs containing breaths on either VC or PC mode exclusively. In these epochs, Pmus and its pressure-time product (PmusPTP) were computed and correlated with Paw's pressure-time product (PawPTP) and VT, respectively. There was a strong correlation of PmusPTP with PawPTP in VC mode (R² = 0.91 [0.76, 0.96]; n = 17,648 epochs) and with VT in PC mode (R² = 0.88 [0.74, 0.94]; n = 33,620 epochs), confirming the hypothesis. As expected, negligible correlations were observed between PmusPTP and VT in VC mode (R² = 0.03) and between PmusPTP and PawPTP in PC mode (R² = 0.06). The study supports the feasibility of assessing respiratory effort during MV non-invasively through airway signal analysis. Further research is warranted to validate this method and investigate its clinical applications.

Association between self-reported METs and other perioperative cardiorespiratory fitness assessment tools in abdominal surgery—a prospective cross-sectional correlation study

Cardiovascular complications represent a significant proportion of adverse events during the perioperative period, necessitating accurate preoperative risk assessment. This study aimed to investigate the association between well-established risk assessment tools and self-reported preoperative physical performance, quantified by metabolic equivalent (MET) equivalents, in high-risk patients scheduled for elective abdominal surgery. A prospective cross-sectional correlation study was conducted, involving 184 patients admitted to a Gastrointestinal Surgery Department. Various risk assessment tools, including the Revised Cardiac Risk Index (RCRI), Surgical Mortality Probability Model (S-MPM), American University of Beirut (AUB)-HAS2 Cardiovascular Risk Index, and Surgical Risk Calculator (NSQIP-MICA), were utilized to evaluate perioperative risk. Patients self-reported their physical performance using the MET-REPAIR questionnaire. The findings demonstrated weak or negligible correlations between the risk assessment tools and self-reported MET equivalents (Spearman’s ρ = − 0.1 to − 0.3). However, a statistically significant relationship was observed between the ability to ascend two flights of stairs and the risk assessment scores. Good correlations were identified among ASA-PS, S-MPM, NSQIP-MICA, and AUB-HAS2 scores (Spearman’s ρ = 0.3–0.8). Although risk assessment tools exhibited limited correlation with self-reported MET equivalents, simple questions regarding physical fitness, such as the ability to climb stairs, showed better associations. A comprehensive preoperative risk assessment should incorporate both objective and subjective measures to enhance accuracy. Further research with larger cohorts is needed to validate these findings and develop a comprehensive screening tool for high-risk patients undergoing elective abdominal surgery.

Genetic overlap between Alzheimer's disease and immune-mediated diseases: an atlas of shared genetic determinants and biological convergence.

The occurrence of immune disease comorbidities in Alzheimer's disease (AD) has been observed in both epidemiological and molecular studies, suggesting a neuroinflammatory basis in AD. However, their shared genetic components have not been systematically studied. Here, we composed an atlas of the shared genetic associations between 11 immune-mediated diseases and AD by analyzing genome-wide association studies (GWAS) summary statistics. Our results unveiled a significant genetic overlap between AD and 11 individual immune-mediated diseases despite negligible genetic correlations, suggesting a complex shared genetic architecture distributed across the genome. The shared loci between AD and immune-mediated diseases implicated several genes, including GRAMD1B, FUT2, ADAMTS4, HBEGF, WNT3, TSPAN14, DHODH, ABCB9, and TNIP1, all of which are protein-coding genes and thus potential drug targets. Top biological pathways enriched with these identified shared genes were related to the immune system and cell adhesion. In addition, in silico single-cell analyses showed enrichment of immune and brain cells, including neurons and microglia. In summary, our results suggest a genetic relationship between AD and the 11 immune-mediated diseases, pinpointing the existence of a shared however non-causal genetic basis. These identified protein-coding genes have the potential to serve as a novel path to therapeutic interventions for both AD and immune-mediated diseases and their comorbidities.

Measuring semantic memory using associative and dissociative retrieval tasks.

Recent theoretical advances highlighted the need for novel means of assessing semantic cognition. Here, we introduce the associative-dissociative retrieval task (ADT), positing a novel way to test inhibitory control over semantic memory retrieval by contrasting the efficacy of associative (automatic) and dissociative (controlled) retrieval on a standard set of verbal stimuli. All ADT measures achieved excellent reliability, homogeneity, and short-term temporal stability. Moreover, in-depth stimulus level analyses showed that the associative retrieval is easier for words evoking few but strong associates, yet such propensity hampers the inhibition. Finally, we provided critical support for the construct validity of the ADT measures, demonstrating reliable correlations with domain-specific measures of semantic memory functioning (semantic fluency and associative combination) but negligible correlations with domain-general capacities (processing speed and working memory). Together, we show that ADT provides simple yet potent and psychometrically sound measures of semantic memory retrieval and offers noteworthy advantages over the currently available assessment methods.

Exploring uses for an algorithmically generated Animal Welfare Indicator for welfare assessment of dairy herds

On-farm welfare assessment is time-consuming and costly. Assessing welfare using routinely collected herd data has been proposed as a more economical alternative. The online Animal Welfare Indicator (AWI), developed by a Norwegian dairy cooperative, applies an algorithm to routinely collected health, production, and management data to "indicate" aspects of animal welfare at herd level. The overall AWI score is based on 10 AWI subindicator scores, representative of elements of animal welfare such as claw health, udder health, and mortality. Our cross-sectional study explored 2 ways in which the AWI may enable more efficient welfare assessment of Norwegian dairy herds. First, we investigated using the AWI to reduce the duration of on-farm assessments by replacing on-farm measures. Second, we examined reducing the number of on-farm welfare assessments by using the AWI to predict which herds have poorer welfare with respect to specific on-farm measures. Using Spearman rank analyses, we investigated if the AWI scores for 157 herds were associated with 24 on-farm welfare variables measured contemporaneously by Welfare Quality assessment. The mortality AWI subindicator score and the percentage mortality in the previous 12 mo were moderately correlated, as were the udder health AWI subindicator score and the percentage high somatic cell count (SCC) in the previous 3 recordings. Only negligible or weak correlations were found between the other AWI scores and the on-farm assessment variables. We built Generalized Linear Models using AWI scores as independent variables to predict herds with poorer welfare. Herds were classified as having poorer welfare based on their results in specific on-farm welfare measures. We evaluated the models' predictive ability and accuracy. Moderately accurate models were built for predicting poorer herds with respect to high SCC, mortality, and moderate or severe lameness. The other models were less accurate. The AWI scores were generally unsuitable as replacements of on-farm welfare measures. The AWI subindicators for udder health and mortality could replace the on-farm welfare measures related to those 2 topics, but there was some overlap in the data used to calculate them. Despite a lack of independence, the use of those 2 AWI subindicators may marginally reduce the duration of on-farm assessments. A prediction model based on AWI scores showed potential for identifying herds with poorer welfare in terms of moderate or severe lameness, facilitating more efficient use of resources for on-farm lameness assessment. As a consequence of the data used in the AWI, it was only reflective of health-related welfare outcomes.

Effect of macular pigment optical density on Yellow‐Blue and Red‐Green colour discrimination

The macular pigment (MP) absorbs preferentially short‐wavelength light and its narrow spectral absorption curve overlaps mostly the spectral responsivity function of S‐cones, although the M‐ and L‐cones are also affected. The MP is restricted to the centre ~14° disc region of the retina with a peak optical density (OD) at the centre in the range 0 to 1 log units. Although the macular pigment optical density (MPOD) decreases rapidly with increasing eccentricity, carotenoid supplementation studies reveal a large uptake of lutein and zeaxanthin, particularly in the parafoveal region and covering the centre ~6° to 8° of the fovea (doi: 10.1111/j.1475‐1313.2006.00386.x).Since the MP contributes to the reduced excitation of S‐cones, it is of interest to examine the effects MP may have on colour vision, particularly when ‘blue‐blocking’ lenses are also involved. We studied the relationship between MPOD and red/green (RG) and yellow/blue (YB) colour thresholds by measuring the spatial distribution of the MP in normal trichromats using the Macular Assessment Profile (MAP) test (doi: 10.1111/j.1475‐1313.2010.00748.x) and the corresponding RG and YB colour thresholds using the CAD test (doi:10.1093/bmb/ldx007).The results show negligible correlation between peak MPOD and YB thresholds and a small, but significant negative correlation with RG thresholds. This observation is of interest since increased MPOD values result in lower RG thresholds. The same experiment was also carried out in subjects with significantly larger amounts of MP following 3 months of supplementation with carotenoids. When averaged over the centre disc region of 2.5° and then over a parafoveal annulus between 2.5° and 4°, the corresponding, mean MP densities show only negligible correlations with RG and YB colour thresholds. To explain these findings, we calculated the expected cone‐contrasts for S‐, M‐ and L‐cones for chromatic displacements defined by the threshold ellipse. The results show that MP absorption of short‐wavelength light has a negligible effect on S‐cone contrast over the whole range of displacement directions associated with the colour threshold ellipse. Repeated calculations with simulated MPOD values up to 1 log unit, produced the same result. More recently, we modelled the effects ‘blue‐blocking’ lenses and pre‐receptor filters in the eye to explore changes to the orientation and the size of the colour threshold ellipse. The model predicts well the measured RG and YB thresholds and confirms the absence of significant threshold changes for MPOD values in the range 0 to 1 log unit. The model does, however, predict systematic changes in the orientation of the major axis (i.e., the tritan axis) and MPOD. These observations are of interest since the orientation of the major axis of the best‐fit ellipse appears to decrease monotonically from ~75° to 55°, as MPOD increases from very small values up to a peak OD of 1 log unit. More work is needed to investigate how YB and RG chromatic sensitivity is affected by combined absorption of short‐wavelength light by MPOD, the lens of the eye and ‘blue‐blocking’ spectacle lenses.

Potential for quantifying general environmental resilience of dairy cattle in sub-Saharan Africa using deviations in milk yield.

Introduction: Genetic improvement of general resilience of dairy cattle is deemed as a part of the solution to low dairy productivity and poor cattle adaptability in sub-Saharan Africa (SSA). While indicators of general resilience have been proposed and evaluated in other regions, their applicability in SSA remains unexplored. This study sought to test the viability of utilizing log-transformed variance (LnVar), autocorrelation (rauto), and skewness (Skew) of deviations in milk yield as indicators of general resilience of dairy cows performing in the tropical environment of Kenya. Methods: Test-day milk yield records of 2,670 first-parity cows performing in three distinct agroecological zones of Kenya were used. To predict expected milk yield, quantile regression was used to model lactation curve for each cow. Subsequently, resilience indicators were defined based on actual and standardized deviations of observed milk yield from the expected milk yield. The genetic parameters of these indicators were estimated, and their associations with longevity and average test-day milk yield were examined. Results: All indicators were heritable except skewness of actual and standardized deviation. The log-transformed variance of actual (LnVar1) and standardized (LnVar2) deviations had the highest heritabilities of 0.19 ± 0.04 and 0.17 ± 0.04, respectively. Auto-correlation of actual (rauto1) and standardized (rauto2) deviations had heritabilities of 0.05 ± 0.03 and 0.07 ± 0.03, respectively. Weak to moderate genetic correlations were observed among resilience indicators. Both rauto and Skew indicators had negligible genetic correlations with both longevity and average test-day milk yield. LnVar1 and LnVar2 were genetically associated with better longevity (rg = -0.47 ± 0.26 and -0.49 ± 0.26, respectively). Whereas LnVar1 suggested that resilient animals produce lower average test-day milk yield, LnVar2 revealed a genetic association between resilience and higher average test-day milk yield. Discussion: Log transformed variance of deviations in milk yield holds a significant potential as a robust resilience indicator for dairy animals performing in SSA. Moreover, standardized as opposed to actual deviations should be employed in defining resilience indicators because the resultant indicator does not inaccurately infer that low-producing animals are inherently resilient. This study offers an opportunity for enhancing the productivity of dairy cattle performing in SSA through selective breeding for resilience to environmental stressors.

National progress towards supranational climate & energy goals due to policies & their mixes? Insights from Northern & Western Europe

The European Union's climate strategy obliges its Member States to fulfil common Climate and Energy Targets by 2030 while the Member States are free in their choice of mixes among polices and instruments providing rule-, economic-and information-focussed incentives. This paper comparatively firstly calculates in how far 11 Member States have already progressed towards these targets, based on the goals set in the National Climate and Energy Plans. Secondly, the paper quantitatively investigates in how far there is a relationship between this progress and the use of different policy-mixes of the countries. The findings about the relationship between progress and policy mixes show that most Pearson correlations (weak to moderate) exist for the rule-focussed incentives (r = −0,41 to −0,62), while fewer but stronger correlations exist for economic- (r = −0,79) and information-focussed incentives (r = −0,73 to −0,74). Voluntary and other policies and measures seem to be of less importance as they only show negligible correlations. Since all discovered correlations are negative, this research shows that a good policy-mix should consist out of a fair share of the main three policy-groups. These highly current findings can guide also other countries globally in the development of a good climate strategy.

Salivary androgens in adolescence and their value as a marker of puberty: results from the SCAMP cohort.

Salivary androgens represent non-invasive biomarkers of puberty that may have utility in clinical and population studies. To understand normal age-related variation in salivary sex steroids and demonstrate their correlation to pubertal development in young adolescents. School-based cohort study of 1495 adolescents at two time points for collecting saliva samples approximately 2 years apart. The saliva samples were analyzed for five androgens (testosterone, androstenedione (A4), 17-hydroxyprogesterone, 11-ketotestosterone and 11β-hydroxyandrostenedione) using liquid chromatography-mass spectrometry; in addition, salivary dehydroepiandrosterone (DHEA) and oestradiol (OE2) were analysed by ELISA. The pubertal staging was self-reported using the Pubertal Development Scale (PDS). In 1236 saliva samples from 903 boys aged between 11 and 16 years, salivary androgens except DHEA exhibited an increasing trend with an advancing age (ANOVA, P < 0.001), with salivary testosterone and A4 concentration showing the strongest correlation (r = 0.55, P < 0.001 and r = 0.48, P < 0.001, respectively). In a subgroup analysis of 155 and 63 saliva samples in boys and girls, respectively, morning salivary testosterone concentrations showed the highest correlation with composite PDS scores and voice-breaking category from PDS self-report in boys (r = 0.75, r = 0.67, respectively). In girls, salivary DHEA and OE2 had negligible correlations with age or composite PDS scores. In boys aged 11-16 years, an increase in salivary testosterone and A4 is associated with self-reported pubertal progress and represents valid non-invasive biomarkers of puberty in boys.

EARLY RECOVERY FOLLOWING TOTAL HIP ARTHROPLASTY IS ASSOCIATED WITH THE ADJACENT MUSCLE QUALITY

Patients demonstrate distinct trajectories of recovery after THA. The purpose of this study was to assess the impact of adjacent muscle quality on postoperative hip kinematics. We hypothesized that patients with better adjacent muscle quality (less fatty infiltration) would have greater early biomechanical improvement.Adults undergoing primary THA were recruited. Preoperative MRI was obtained and evaluated via Scoring Hip Osteoarthritis with MRI Scores (SHOMRI, Lee, 2015). Muscle quality was assessed by measuring fat fraction [FF] from water-fat sequences. Biomechanics were assessed preoperatively and six weeks postoperatively during a staggered stance sit-to-stand using the Kinematic Deviation Index (KDI, Halvorson, 2022). Spearman's rho was used to assess correlations between muscle quality and function.Ten adults (5M, 5F) were recruited (average age: 60.1, BMI: 23.79, SHOMRI: 40.6, KDI: 2.96). Nine underwent a direct anterior approach and one a posterior approach. Preoperatively, better biomechanical function was very strongly correlated with lower medius FF (rho=0.89), strongly correlated with lower FF in the minimus (rho=0.75) and tensor fascia lata (TFL) FF (rho=0.70), and weakly correlated with SHOMRI (rho=0.29). At six weeks, greater biomechanical improvement was strongly correlated with lower minimus FF (rho=0.63), moderately correlated with medius FF (rho=0.59), and weakly correlated with TFL FF (rho=0.26) and SHOMRI (rho=0.39). Lastly, medius FF was moderately correlated with SHOMRI (rho=0.42) with negligible correlations between SHOMRI and FF in the minimus and TFL.These findings suggest adjacent muscle quality may be related to postoperative function following THA, explaining some of the variability and supporting specialized muscle rehabilitation or regeneration therapy to improve outcomes.

Mothers' symptoms of anxiety and depression and the development of child temperament: A genetically informative, longitudinal investigation.

Child temperament traits and mothers' emotional symptoms relating to anxiety and depression may drive changes in one another, leading to their 'co-development' across time. Alternatively, links between mother and child traits may be attributable to shared genetic propensities. We explored longitudinal associations between mothers' emotional symptoms and child temperament traits and adjusted for genetic effects shared across generations. This study is based on the Norwegian Mother, Father and Child Cohort Study (MoBa). Mothers (n=34,060) reported on their symptoms of anxiety and depression, and temperament among offspring (n=42,526), at child ages 1.5, 3 and 5years. Structural equation models parameterised developmental change in traits, and an extended family design adjusted for genetic effects. We found individual differences in stable trait scores and rate of change for all study variables. Longitudinal stability in mothers' emotional symptoms was associated with longitudinal stability in offspring emotionality (r=0.143), shyness (r=0.031), and sociability (r=-0.015). Longitudinal change in mothers' symptoms showed very small or negligible correlations with longitudinal change in child temperament. Both genetic and environmental influences explained the stable longitudinal association between mothers' symptoms and child emotionality. The studied associations between mother and child traits across time appeared to be due to stable, trait-like factors, involving genetic and environmental influence, rather than their co-development. Findings contribute knowledge on how emotional symptoms develop in families across time, and the methods with which we can explore such development.

Computed Tomographic Assessment of Pituitary Gland Dimensions in Domestic Short-Haired Cats.

The detection of subtle changes in the pituitary dimensions has relevant clinical implications. In cats, a few studies have established the cut-off values of the pituitary gland's dimensions using small and inhomogeneous samples. The aims of this study were: to determine by computed tomography (CT) the pituitary linear dimensions and the pituitary-to-brain (P:B) ratio in a sample of domestic short-haired (DSH) cats; to assess the effects of sex, age, and weight on pituitary dimensions; and to evaluate the inter- and intra-observer agreement for such measurements. All skull CTs of DSH cats performed over four years using a multidetector CT and a standardized protocol were retrospectively reviewed. The exclusion criteria were: clinical, laboratory, or CT alterations of the pituitary gland, brain diseases, fractures of the neurocranium, and diabetes. The pituitary dimensions and brain area were assessed by two different observers using multiplanar reconstructions and automated segmentation tools. Fifty-one cats were included in the final sample. The intraclass correlation coefficients for intra- and inter-observer reliability were good/excellent, and moderate/good, respectively. No differences between sexes were detected, and negligible correlations were found between age and weight. According to this study, a pituitary gland with a height > 4 mm or a P:B ratio > 0.49 mm should be considered enlarged.