Negative Rh Research Articles

Genotyping for Weak and Partial D Rh Status to Optimize Usage of Limited Blood Supplies

Abstract Introduction The Rh antigen group, expressed on erythrocytes and encoded by highly polymorphic RHD and RHCE genes on chromosome 1 (1p36-p34), is notable in transfusion medicine due to its high polymorphism, strong immunogenicity and ability to cause hemolytic transfusion reactions in patients and hemolytic disease of the fetus and newborn (HDFN). The D antigen specifically contains multiple epitopes with variations that include decreased expression (weak D) or loss of certain epitopes (partial D) which expose patients to risk of alloimmunization depending on the subtype of the Rh(D) antigen. Hence, serologic weak D testing followed by molecular testing is not performed except for donors testing negative for Rh(D) antigen and newborns typing negative for Rh(D) and born to Rh(D) negative mothers. Given the current shortages of blood supplies, understanding patients’ genotypes in the context of weak or partial D-typing and risk of alloimmunization can be essential in the management of increasingly limited supplies of red blood cell (RBC) units. Objective To further characterize patients that initially type negative for Rh(D) but positive for weak D with molecular testing to investigate risk of alloimmunization, retype the Rh(D) and avoid unnecessary use of Rh(D) negative RBC units during critical shortages of RBC units. Methods We collected the results of ABO/Rh(D) typing for patients with negative Rh(D) testing but positive weak D test over a period of four weeks in December 2023 and had D-variant molecular testing performed by a reference laboratory. Initial serologic ABO/Rh(D) was performed in tube followed by weak D testing by indirect Coombs test in tube. Results The total number of patients who initially tested negative for Rh(D) but positive (weak to +4) for weak-D was three patients. Two patients showed a probable genotype of RHD*01W.1 (RHD* weak type 1, 809T>G (V270G)) homozygous which indicated the presence of weak D+ type 1. Patients with weak D+ type 1 can receive Rh(D) positive red cell units, and they are not at risk of alloimmunization and developing anti-D. The third patient showed a probable genotype RHD*DVI type 2 homozygous (lack of Rh(D) exons 4 through 6) and indicated the presence of partial D antigen. Patients with partial D antigen should receive RH(D) negative red cell units as they are at risk of alloimmunization and developing anti-D. Conclusions Although the vast majority of Transfusion Medicine services do not perform the serologic weak D test with reflex to molecular testing, characterizing patients who test negative with initial serologic Rh(D) testing with serologic and molecular D-tests may aid in retyping the Rh(D) of patients during critical shortages, particularly for patients with ABO blood group O.

Prevalence of Rhesus D negativity and barriers to immuno- prophylaxis among obstetric patients in Kitengela Sub- county Hospital: A cross-sectional study

Background: Rhesus (Rh) alloimmunization is a serious but preventable disease that develops in women who are Rh(D)-negative. This disease contributes significantly to perinatal morbidity and mortality. Prevalence data of Rh(D)-negative blood type among pregnant women in Kenya are scarce. This study aimed to determine the prevalence of Rh(D)-negative blood type, barriers to immunoprophylaxis, and ABO blood type patterns among the obstetric population in Kitengela Subcounty Hospital, Kajiado, Kenya. Methods: A cross-sectional study design was employed in which medical records of Rh-negative women as documented at the first antenatal visit were retrieved. Data were collected, extracted into a data collection tool, and analyzed using the Statistical Package for Social Scientists Software (SPSS version 23). Results: Of the 7141 women screened, 1.2 % (85) were Rh-negative. Among these, blood group O was the most common (48.2%), followed by blood groups B (24.7%), A (23.5%), and AB (3.5%). Most Rh-negative women (45.9%) were aged 15-23 years. Following delivery, only half of the women received anti-D immunoglobulin. Among those who did not receive anti-D postnatally, 80.8% lacked a documented reason, whereas 12.2% and 4.9% of the patients lacked access and did not know their Rh status, respectively. None of these patients had fetal blood grouping conducted before anti-D administration. Conclusion: Rh(D) negativity remains prevalent in the Kenyan population. However, access restrictions at both diagnostic and therapeutic levels remain. In addition, there is an overt hiatus in neonatal blood grouping.

IJCM_99A: Risk Factors Associated with Polyhydramnios among Pregnant Women in Mangalore: A Case Control Study

Background: The amniotic fluid volume is maintained by a balance between production (fetal urine and fetal lung fluid) and resorption (swallowing and intramembranous flow). Any imbalance in this process could lead to either oligohydramnios or polyhydramnios. The incidence of polyhydramnios varies from 1-2%. It is a significant obstetric condition, the etiology of which is still largely unknown. The underlying maternal factors include diabetes mellitus, pregnancy induced hypertension, twin pregnancy, negative Rh factor, cardiac and renal diseases. The study aims to study the risk factors associated with polyhydramnios among the women seeking maternal health care in the study hospitals and to assess the socio-demographic correlates of polyhydramnios among the study population. Methodology: The study was conducted in the Government Lady Goschen Hospital and Kasturba Medical College Attavar, among 66 cases (Pregnant women admitted with polyhydramnios) and 132 controls(age matched pregnant women admitted to the study hospitals during the study period). Odds ratio was calculated, Chi square test was used to compare the groups. Results: Proportion of anemia among our cases was 20% and control was 25.6%. 7.6% of the cases were associated with hypertensive disorders of pregnancy and 0.8% of controls had hypertensive disorders. 12.1% of polyhydramnios cases with Gestational Diabetes Mellitus Conclusion: Majority of the polyhydramnios cases were in the age group 20-29 years. The proportion of Diabetes Mellitus, hypertension, Rh negative pregnancies, malpresentation were higher among polyhydramnios cases. The association was significant for hypertension.

Hepatitis C Virus Infection and ABO Blood Group Assessment: Tertiary Hospital Study in India.

People with certain blood groups and Rh positive are more prone to infections transmitted by blood transfusion. The aim of this research was to survey the accompaniment of ABO Blood Group System and Rh type with infection to hepatitis C virus in India. This was a retrospective study in patients during October 2019-March2022 in India. The population of blood donors was tested for blood borne infections, including HCV. Logistic regression was used and collected data were analyzed using SPSS v.16.A total number of 901 people referred to the organization for donating blood during aforementioned years. Of these, 224 people had a history of hepatitis C disease, including 189 unmarried persons and the rest were married. 167 individuals were males and 57individuals were females. People who had viral diseases were comprised of 76 persons with negative Rh and 148positive persons with Rh.Future aims should include studies into blood groups and Rh types, according to the results of this study, in order to avoid the spread of blood-borne infections. Furthermore, further study is needed to establish the particular blood kinds that provide an elevated danger for classified donors.

Association Between Rhesus and ABO Blood Group Types and Their Impact on Clinical Outcomes in Critically Ill Patients with COVID-19: A Multi-Center Investigation.

There is increasing evidence suggesting that ABO blood type may play a role in the immunopathogenesis of COVID-19 infection. In addition to ABO blood type, the Rhesus (Rh) factor has also been implicated in various disease processes. Therefore, our study aimed to assess the association between both ABO and Rh blood types in critically ill patients with COVID-19 and their clinical outcomes. A multicenter retrospective cohort study conducted in Saudi Arabia between March 1, 2020, and July 31, 2021, involving adult COVID-19 patients admitted to Intensive Care Units, aimed to explore potential associations between rhesus blood group types (Positive versus Negative) and clinical outcomes. The primary endpoint assessed was the hospital length of stay (LOS). Other endpoints were considered secondary. After propensity score matching (3:1 ratio), 212 patients were included in the final analysis. The hospital length of stay was longer in a negative Rh blood group compared with patients in the Rh-positive group (beta coefficient 0.26 (0.02, 0.51), p = 0.03). However, neither 30-day mortality (HR 0.28; 95% CI 0.47, 1.25, p = 0.28) nor in-hospital mortality (HR 0.74; 95% CI 0.48, 1.14, p = 0.17) reached statistical significance. Additionally, among the different ABO types, the A+ blood group exhibited a higher proportion of thrombosis/infarction and in-hospital mortality (28.1% and 31.2%, respectively). This study highlights the potential impact of blood group type on the prognosis of critically ill patients with COVID-19. It has been observed that patients with a negative Rh blood group type tend to have a longer hospital stay, while their mortality rates and complications during ICU stay are similar to the patients with a Rh-positive group.

Effect of Rhesus factor incompatibility on maternal outcome (fertility): A comprehensive review

Rh incompatibility, also known as Rh disease, is defined as a condition that occurs when a woman with Rh-negative blood type is exposed to Rh-positive blood cells, leading to the development of Rh antibodies. The incidence of occurrence is more frequently among those of Caucasian (North American and European) descent (15% to 17%) compared to those of African (4% to 8%) or Asian descent (0.1% to 0.3%). Worldwide, the prevalence of Rh disease is estimated to be 276 per 100,000 live births, which is significant considering that an estimated 50% of untreated cases of haemolytic disease of the neonate (HDN). HDN will either die or develop brain damage due to the disease. The condition is due to: If a Rhesus negative (Rh-) woman is impregnated by a man with Rhesus negative (Rh-) there wouldn’t be any problem. If a Rhesus negative woman (Rh-) is impregnated by a man with Rhesus positive (Rh+) and the baby inherited the rhesus positive (Rh+) from the father there will be a problem or when an Rh-negative mother is exposed to the Rh D antigen, the D antigen is perceived as a foreign threat leading to the haemolysis of the fetal erythrocytes, common signs and symptom include; Jaundice, a yellowing of the skin and whites of the eyes, lethargy, heart failure, enlarged organs. It can be prevented and treated by administering an injection of RhoGAM during the second trimester, and 28th week of pregnancy respectively, exchange transfusions either before birth or after delivery. Phototherapy is also another treatment modality which break down excess bilirubin into less toxic substance that the new borns liver can remove.

Rh negativity seems to predispose to a milder COVID-19 course

Rh negativity seems to predispose to a milder COVID-19 course

#5 : Associated Factors with Premenstrual Syndrome and Premenstrual Dysphoric Disorder Among Female Medical Students: A Cross-Sectional Study

Background and Aims: Premenstrual syndrome (PMS) and Premenstrual dysphoric disorder (PMDD) are two premenstrual disorders that have been reported in many countries and have gradually become predominant concerns The study aimed to determine potential risk factors associated with PMS and PMDD Method: Three hundred two female student participants who were 18 – 45 years old completed a questionnaire including demographic characteristics, lifestyle factors, and a Vietnamese Premenstrual Syndrome Screening Tool (PSST). We then followed up participants during at least two menstrual cycles using the Daily Record of Severity of Problems (DRSP). The PMS and PMDD diagnosis were established using The Carolina Premenstrual Assessment Scoring System (C-PASS), based on the American College of Obstetrics and Gynecology (ACOG) and Diagnostic and Statistical Manual of Mental Disorders (DSM-5). Results: According to the C-PASS, 35 out of 302 students (11.6%; 95%CI: 8.2-15.7%) met the diagnosis of PMS (31 students) or PMDD (4 students). We found that age at menarche (PR = 0.77, 95%CI: 0.63 - 0.96), having negative Rh blood type (PR=4.43, 95%CI: 1.95 to 10.08), being moderately depressed or higher (PR=2.81, 95%CI: 1.24 to 6.36), and consuming caffeine more than three times per week were statistically associated with having PMS/PMDD after adjusting for other variables. Conclusion: The prominent risk factors for PMS and PMDD were negative Rhesus blood type, menarche age, caffeine consumption, and self-reported depression.

Electrical Properties of Heavily Al-Doped 4H-SiC

We investigate the temperature-dependent resistivity (ρ(T)) and Hall coefficient (RH(T)) of heavily Al-doped 4H-SiC and discuss the underlying conduction mechanisms. The sign of RH(T) changes from positive to negative in nearest-neighbor hopping (NNH) and variable-range hopping (VRH) conduction, whereas it is positive in band conduction because Al-doped 4H-SiC is a p-type semiconductor. We propose a general physical model to explain why RH(T) in hopping conduction becomes negative at low temperatures, which is applicable to both NNH and VRH conduction. Moreover, we elucidate why the activation energy for negative RH(T) becomes similar to that of ρ(T) in NNH conduction.

Association between Rhesus Blood Groups and Malaria Infection: A Systematic Review and Meta-Analysis.

In the literature, there was inconsistency in the risk of malaria between individuals with Rhesus blood group positive (Rh+) and negative (Rh-). The systematic review aimed to investigate the risk of malaria among participants with different Rh blood types. All observational studies that reported the occurrence of Plasmodium infection and investigation of the Rh blood group were searched in five databases (Scopus, EMBASE, MEDLINE, PubMed, and Ovid). Strengthening the Reporting of Observational Studies in Epidemiology was used to assess the reporting quality in the included studies. A random-effects model was used to calculate the pooled log OR and 95% confidence intervals (CIs). Database searches yielded a total of 879 articles, of which 36 were eligible for inclusion in the systematic review. The majority of the included studies (44.4%) revealed that Rh+ individuals had a lower proportion of malaria than Rh- individuals; however, the remaining studies revealed a higher or no difference in the proportion of malaria between Rh+ and Rh- individuals. Overall, with moderate heterogeneity, the pooled results showed no difference in malaria risk between patients with Rh+ and Rh- (p = 0.85, pooled log OR: 0.02, 95% CI: -0.20-0.25, I2: 65.1%, 32 studies). The current study found no link between the Rh blood group and malaria, even though there was a moderate amount of heterogeneity. Further studies using prospective designs and a definitive method for Plasmodium identification are needed to investigate the risk of Plasmodium infection in Rh+ individuals and increase the reliability and quality of these studies.

The Distribution and Phenotypes of Blood Groups in Hematologic Malignancies.

Blood groups are associated with duodenal ulcer, diabetes mellitus, and urinary tract infection. In some studies, a relationship was detected between hematologic and solid organ malignancies and blood groups. In this study, we investigated the frequency and phenotypes of blood groups (ABO, Kell, Duffy, Rh) in patients with hematologic malignancies. One hundred sixty-one patients with hematologic malignancy (multiple myeloma, chronic lymphocytic leukemia, and chronic myelocytic leukemia) and 41 healthy people were evaluated prospectively. We determined phenotypes and distribution of ABO, Rh, Kell, and Duffy blood groups in all cases. Chi-square test and 1-way variance analysis were used for statistical analysis. P < .05 value was considered statistically significant. In patients with multiple myeloma, the A blood group was statistically significantly more frequent than in the control group (P = .021). Rh negativity was found more frequent in patients with hematologic malignancy than the control group (P = .009). Kpa and Kpb antigen positivity were found statistically significantly less frequent in patients with hematologic malignancy (P = .013, P = .007; respectively). Fy (a-b-) and K-k+ phenotypes were higher in patients with hematologic cancer than in the control group (P = .045). We determined a significant relationship between hematologic malignancies and blood group systems. In our study, due to the low number of cases and few hematological malignancy types, extensive studies with more cases and more hematologic cancer types are needed.

Associated factors with Premenstrual syndrome and Premenstrual dysphoric disorder among female medical students: A cross-sectional study.

The study aimed to determine potential risk factors associated with Premenstrual Syndrome and Premenstrual Dysphoric Disorder. Three hundred two female student participants who were 18-45 years old completed a questionnaire including demographic characteristics, lifestyle factors, and a Vietnamese Premenstrual Syndrome Screening Tool. We then followed up participants during at least two menstrual cycles using the Daily Record of Severity of Problems. The Premenstrual Syndrome and Premenstrual Dysphoric Disorder diagnosis was established using The Carolina Premenstrual Assessment Scoring System, based on the American College of Obstetrics and Gynecology and Diagnostic and Statistical Manual of Mental Disorders. According to the Carolina Premenstrual Assessment Scoring System, 35 out of 302 students (11.6%; 95%CI: 8.2-15.7%) met the diagnosis of PMS (31 students) or PMDD (4 students). We found that age at menarche (PR = 0.77, 95%CI: 0.63-0.96), having negative Rh blood type (PR = 4.43, 95%CI: 1.95 to 10.08), being moderately depressed or higher (PR = 2.81, 95%CI: 1.24 to 6.36), and consuming caffeine more than three times per week were statistically associated with having Premenstrual Syndrome or Premenstrual Dysphoric Disorder after adjusting for other variables. The prominent risk factors for Premenstrual Syndrome and Premenstrual Dysphoric Disorder were negative Rhesus blood type, menarche age, caffeine consumption, and self-reported depression.

Association between ABO blood group and COVID-19-positive patients in institutional-based hospital, Coimbatore – A cross-sectional study

Objective: The novel coronavirus disease (COVID-19) has spread globally and caused 6.5 million death as of October 2022. Risk factors for COVID-19 morbidity and mortality include age, sex, smoking, hypertension, cardiovascular disorders, respiratory disease, and diabetes mellitus. Association between ABO blood groups and number of other infections or disease severity following infections such as SARS-COV-1 and hepatitis B virus has been reported. Rh(D) phenotype (positive and negative Rh blood types) is associated with very few diseases compared to ABO blood group. Various articles have reported the association of ABO blood group and COVID-19. Our aim was to investigate if any association exists in between ABO blood group with Rh phenotype and COVID-19 infection in Coimbatore and with the result an awareness among the public should be created to hasten the preventive measure from further encounter of the infection and help the government in taking a proactive approach to fight COVID-19 in the area. Thus, we conducted a study to find out the association between ABO blood group and COVID-19-positive (SARS-COV-2) patients in institutional-based hospital – Coimbatore which was one of the most affected districts in Tamil Nadu. Materials and Methods: This is a single-center, retrospective, observational, and cross-sectional study conducted in a private institutional-based hospital in Coimbatore. The data were collected from around 437 patients’ medical records, who were tested COVID-19-positive between October 2020 and November 2021 whose blood grouping was available as part of investigation. Data were analyzed statistically to correlate the association of ABO blood group, Rh phenotype, and COVID-19 infection. Results: In 437 COVID-19-infected patients, the ABO blood group displayed a percentage distribution for A, B, AB, and O at 19% (83), 35% (153), 8.5% (37), and 37.5% (164), respectively and 409 (93.6%) were Rh +ve. Among the patients, blood group O 164 (37.5%), followed by blood group B153 (35%) is observed to be significantly associated with COVID-19 susceptibility, whereas blood group A83 (19%) and AB 37(8.5%) showed lower susceptibility for COVID-19 infection. Conclusion: This study did not show any statistically significant association of ABO blood group and Rh phenotype seen in relation to COVID-19 infection.

Rhesus Negativity Prevalence and Neonatal Outcomes among Pregnant Women Delivered at Bule Hora University Teaching Hospital, West Guji Zone, South Ethiopia.

Rh incompatibility has been an important cause of severe neonatal hyperbilirubinemia, hydrops fetalis, and stillbirth. Among those outcomes, neonatal jaundice is the most common problem. The study is assessed the prevalence of Rhesus (Rh) negativity and neonatal outcomes among pregnant women who delivered at Bule Hora University Teaching Hospital over a 5-year period from January 2017 to December 31, 2022. A retrospective study was conducted on 110 women who delivered at Bule Hora University Teaching Hospital (BHUTH) from January 2017 to December 31, 2021. The complete data of the mother's and neonates' status were extracted from the registration book of the hospital using checklists. The data were double entered using EpiData version 3 and exported to the Statistical Package for Social Sciences (SPSS) version 26 for analysis. Descriptive statistics to determine prevalence and frequencies were used to describe the study population in relation to relevant variables, and the results are presented in tables and charts. The study shows that the prevalence of Rh D-negative among women who delivered was 6.4% [95% CI: 1.83,10.98]. Among Rh-negative women, 1 (25%) of blood group AB, 3 (6.5%) of blood group O, and 2 (6.1%) of blood group A were Rh-D negative. The distributions of O, A, B, and AB blood groups among pregnant women who delivered this hospital were 41.8%, 30%, 24.6%, and 3.6%, respectively. Out of neonates born to Rh-negative women, 1 (14.3%) was born with jaundice. Of women who delivered at BHUT hospital, 61 (55.5%) did not have a previous delivery, 7 (6.4%) had a previous abortion, 5 (4.5%) stillbirth, 1 (0.9) died after birth, 4 (3.6%) had a birth child weight less than 2.6 kg. The study revealed that the prevalence of Rh-negative was comparable with finding of different similar studies. To reduce Rh incompatibility-related HDN, the government should educate mothers and encourage them as they follow ANC facilities and after delivery to health facilities.

Association of Plasma Metabolites and Lipoproteins with Rh and ABO Blood Systems in Healthy Subjects.

This study investigated the associations between the levels of 27 plasma metabolites, 114 lipoprotein parameters, determined using nuclear magnetic resonance spectroscopy, and the ABO blood groups and the Rhesus (Rh) blood system in a cohort of n = 840 Italian healthy blood donors of both sexes. We observed good multivariate discrimination between the metabolomic and lipoproteomic profiles of subjects with positive and negative Rh. In contrast, we did not observe significant discrimination for the ABO blood group pairwise comparisons, suggesting only slight metabolic differences between these group-specific metabolic profiles. We report univariate associations (P-value < 0.05) between the subfraction HDL1 related to Apo A1, the subfraction HDL2 related to cholesterol and phospholipids, and the particle number of LDL2 related to free cholesterol, cholesterol, phospholipids, and Apo B and the ABO blood groups; we observed association of the lipid main fraction LDL4 related to free cholesterol, triglycerides, and Apo B; creatine; the particle number of LDL5; the subfraction LDL5 related to Apo B; the particle number of LDL4; and the subfraction LDL4 related to Apo B with Rh blood factors. These results suggest blood group-dependent (re)shaping of lipoprotein metabolism in healthy subjects, which may provide relevant information to explain the differential susceptibility to certain diseases observed in different blood groups.

ABO-Rh Blood Types and Clinical Consequences of COVID-19 Infection.

Because of there is no sufficient evidence showing a relationship between blood types and severity of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, this study was planned to investigate the effects of ABO blood group on the clinical outcomes of SARS-CoV-2 infection. The data of the patients were examined retrospectively. The patients who were hospitalized in wards or intensive care unit, constituted the study group. The patients who presented to the hospital because of other causes and whose blood type examinations were performed, were included in the control group. The study group consisted of 406 six patients were diagnosed with SARS-CoV-2 infection. Control group consisted of 38079 patients whose blood group was determined for any reason in the same period. The rate of Rh negativity was significantly higher in the patient group (p = 0,01). Hospitalization duration in intensive care was significantly longer in the blood type A and AB groups compared to the blood type O group (p = 0,03). Our results are in agreement with other studies suggesting that blood group O individuals are somewhat more resistant to clinically overt infection with SARS-CoV-2 than other blood groups. In addition, Rh negativity may also be an individual risk factor for SARS-CoV-2 infection.

Serological Detection of Some Rhesus D Polymorphisms among Rhesus Negative Sudanese Blood Donors

Background: Rhesus (Rh) blood group, mainly the D phenotype is subject to polymorphisms associated with several Rh D variants. The identification of such variants may prevent blood reactions due to Rh incompatibilities. Material and Methods: This descriptive cross-sectional study was conducted in Khartoum North, Khartoum-Sudan during the period from December 2021 to March 2022. A total of 100 blood donors who had Rh D negative grouping results were recruited for this study. All samples were tested for weak D and Rh-Del phenotypes by indirect antihuman globulin and adsorption-elution methods respectively. In addition, Rh CE typing was performed for all samples, then the association between Rh CE phenotype and Rh D variants was tested by Chi square test. P values of &lt; 0.05 were considered statistically significant. Results: Among the tested donors, 7% were Rh-Del, 5% were weak D while the remaining 88% were truly Rh-negative phenotype. All Rh-Del subjects were Rh CE positive; 80% of weak D were Rh CE positive, while only 11% of truly Rh-negative donors were Rh CE positive. Statistically significant association was observed between Rh CE positive phenotype with Rh Del and weak D phenotypes, while truly negative Rh D phenotype is associated with negative Rh CE phenotypes (All P values &lt; 0.05). Discussion: Variant D phenotypes were observed in this study population. Therefore, attention for this category among Rh D negative donors is needed to avoid allo immunization in truly negative patients.

Is there an association between periodontal diseases and ABO blood group? Systematic review and meta-analysis.

To systematically review indexed literature that assessed the association between periodontal diseases and the ABO blood group. The addressed focused question was "Is there an association between periodontal diseases and the ABO blood group?" Indexed databases were searched without time and language restrictions using different key words. Original studies, case reports, and case series that assessed the association between periodontal diseases and the ABO blood group were included in a meta-analysis. The risk of bias was evaluated using the National Institutes of Health tool. Letters to the Editor, commentaries, and historical reviews were excluded. A total of 17 studies including 8,543 individuals were included; periodontitis (n = 4,476), gingivitis (n = 1,582), and healthy (n = 1,610). The most frequent event rate of blood group phenotype in periodontitis was blood group type O 39.0 % (95% CI 0.35 to 0.43). The event rate of positive Rh was 90.7% (95% CI 0.89 to 0.92) compared to 9.2% (95% CI 0.06 to 0.13) for negative Rh. Fixed effect analysis showed that blood group AB had a protective value to periodontitis; odds ratio 0.66 (95% CI 0.50 to 0.80; P < .001). However, there was no significant association of blood group A, B, and O. In addition, there was no significant association between ABO blood group and gingivitis; odds ratios of blood group B and O were 1.18 (95% CI 0.80 to 1.67; P = .34) and 1.36 (95% CI 0.89 to 2.08; P = .16), respectively. There is a discrepancy among published observations regarding the effect of the ABO blood group on periodontium health. Further studies are needed with a large sample population to build more robust evidence. (Quintessence Int 2022;53:404-412; doi: 10.3290/j.qi.b2644845).

Contributing indications that provoke C-section: A study from tertiary care hospitals

Abstract Background: Both cesarean-section (CS) delivery (CD) and vaginal delivery (VD) is associated with well-known measurable short- and long-term maternal and neonatal complications and benefits. Objective: The present retrospective observational study was conducted on a preliminary basis to evaluate the contributing factors that provoke CS delivery. Materials and Methods: The short-term (6 months) retrospective study was conducted at the Department of Obstetrics and Gynaecology, Pt. Deen Dayal Upadhyay and Northern Railway divisional hospital Moradabad, Uttar Pradesh, India. The sample size was calculated accordingly with a relative precision of 10.2% of prevalence and was found to be 140 and we have taken the sample size of 345. Results: The prevalence of CD for a particular course of duration was found to be 36.23%. The risk of CD increased (P &lt; 0.001) with increased BMI. However, differential limits of normal BMI signify that lower limit of normal BMI possessed high % of CS. As the education profile was getting high the possibility of CS was found to be increased (P &lt; 0.001). Women with negative Rh factor the probability of CS in terms of percentage was found to be 87.5%. There was a proportionate possibility of CS with an increased gap between parities. The % mortality of new-born by CD and VD was 14.28% and 85.71%, respectively, which signifies that CS prevents infant mortality. There was 99.2% possibility of undergoing CS if a woman possessing medical complications. Conclusion: The present study acknowledged that CD ensures feto-maternal safety. However, the study also highlighted some probable indications that might engender CD.

Prevalence of Weak D (Du) among blood donors in South-West region of Maharashtra- An Observational study

Background: ABO was the first blood group system to be discovered by Landsteiner in 1901 and then along with Weiner he discovered Rh antigen in 1939 which farther led to description of haemolytic disease of new born by Levine and Stetson. The term Rh positive and Rh negative refers to the presence or absence of D antigen. The incidence of Rh negativity varies between 3%-25% and that of the weak D antigen range from 0.2-1%. This study aimed to find the prevalence of Weak D among blood donors. Materials and methods: ABO and Rh Blood Group of donors was analysed in our blood bank from 2018 to 2020 in South West region of Maharashtra, India. Rh negative individuals were further processed for detection of weak “D” antigen by gel card method using LISS/Coombs reagent. Results: A total of 21823 blood samples were analysed during the period 2018 to 2020. Out of these 20746 (95.06%) were Rh D positive and 1077 (4.93%) were Rh D negative. Out of 1077 Rh negative blood samples 45 (4.1%) were found positive for weak D antigen. Conclusion: The prevalence of Rh-negative blood group is 4.93% and weak D positive is 4.1% among the blood donors of south west region of Maharashtra.