Negative Physiological Effects Research Articles

Effects of dietary glycerol monolaurate on growth and digestive performance, lipid metabolism, immune defense and gut microbiota of shrimp (Penaeus vannamei)

The advancement of the Penaeus vannamei industry in a sustainable manner necessitates the creation of eco-friendly and exceptionally effective feed additives. To achieve this, 720 similarly-sized juvenile shrimp (0.88 ± 0.02 g) were randomly divided into four groups in this study, with each group consisting of three replicates, each tank (400 L) containing 60 shrimp. Four experimental diets were formulated by adding 0, 500, 1000, and 1500 mg kg−1 glycerol monolaurate (GML) to the basal diet, and the feeding trial lasted for 42 days. Subsequently, a 72-h White Spot Syndrome Virus (WSSV) challenge test was conducted. Polynomial orthogonal contrasts analysis revealed that with the increase in the concentration of GML, those indicators related to growth, metabolism and immunity, exhibit linear or quadratic correlations (P < 0.05). The results indicate that the GML groups exhibited a significant improvement in the shrimp weight gain rate, specific growth rate, and a reduction in the feed conversion ratio (P < 0.05). Furthermore, the GML groups promoted the lipase activity and reduced lipid content of the shrimp, augmented the expression of triglyceride and fatty acid decomposition-related genes and lowered the levels of plasma triglycerides (P < 0.05). GML can also enhanced the humoral immunity of the shrimp by activating the Toll-like receptor and Immune deficiency immune pathways, improved the phagocytic capacity and antibacterial ability of shrimp hemocytes. The challenge test revealed that GML significantly reduced the mortality of the shrimp compared to control group. The 16S rRNA sequencing indicates that the GML group can increases the abundance of beneficial bacteria. However, 1500 mg kg−1 GML adversely affected the stability of the intestinal microbiota, significantly upregulating intestinal antimicrobial peptide-related genes and tumor necrosis factor-alpha levels (P < 0.05). In summary, 1000 mg kg−1 GML was proven to enhance the growth performance, lipid absorption and metabolism, humoral immune response, and gut microbiota condition of P. vannamei, with no negative physiological effects.

Influence of stocking density on the growth, immune and physiological responses, and cultivation environment of white-leg shrimp (Litopenaeus vannamei) in biofloc systems

Biofloc (BF) stands out as a promising system for sustainable shrimp farming. Optimizing various culture conditions, such as stocking density, carbohydrate source, and feeding management, is crucial for the widespread adoption of the BF system. This study compares the growth performance of white-leg shrimp (Litopenaeus vannamei) in culture ponds at low density (LD) with 50 organisms/m2 and high density (HD) with 200 organisms/m2. Post-larvae of white-leg shrimp were stocked for 16 weeks in both LD and HD groups. The LD group exhibited a superior survival rate, growth rate, and feed consumption compared to the HD group. The BF from the LD system recorded a significantly higher protein content (16.63 ± 0.21%) than the HD group (15.21 ± 0.34%). Heterotrophic bacterial counts in water did not significantly differ with stocking density. However, Vibrio count in water samples was higher in the HD group (3.59 ± 0.35 log CFU/mL) compared to the LD group (2.45 ± 0.43 log CFU/mL). The whole shrimp body analysis revealed significantly higher protein and lipid content in the LD group. In contrast, the total aerobic bacterial count in shrimp from the HD group was high, with the identification of Salmonella enterica ssp. arizonae. Additionally, Vibrio counts in shrimp samples were significantly higher in the HD group (4.63 ± 0.32 log CFU/g) compared to the LD group (3.57 ± 0.22 log CFU/g). The expression levels of immune-associated genes, including prophenoloxidase, transglutaminase, penaiedin 3, superoxide dismutase, lysozyme, serine proteinase, and the growth-related gene ras-related protein (rap-2a), were significantly enhanced in the LD group. Conversely, stress-related gene expression increased significantly in the HD group. Hepatopancreases amylase, lipase, and protease were higher in the LD group, while trypsin activity did not differ significantly. Antioxidant enzyme activity (catalase, glutathione, glutathione peroxidase, and superoxide dismutase) significantly increased in the LD group. The histological structure of hepatopancreas, musculature, and female gonads remained similar in both densities. However, negative effects were observed in the gills' histology of the HD group. These results suggest that increasing stocking density is associated with significantly negative biological, microbial, and physiological effects on white-leg shrimp under the BF system.

Unpacking Technostress: A Systematic Review on its Effects and Mitigation

The exponential growth of technology in everyday life has given rise to technostress, a condition marked by negative psychological and physiological effects. This systematic review synthesizes current research on technostress, examining its global definition, regional variations, impacts, opportunities and mitigation strategies. Drawing on established theories such as the Technostress Model, Job Demands-Resources Model, and Person-Environment Fit Model, the review explores the complex interplay between technology use and stress. The technostress model posits that technostress arises from negative reactions to technology use, fueled by information overload, task demands, and lack of control. The job demands-resource model views technology as either a stressor or a resource, impacting well-being based on organizational context and individual traits, while the person-environment fit model links technostress to capability-demand mismatches. Studies link technostress to anxiety, depression, and physical health problems. The review underscores the importance of tailored interventions, emphasizing organizational support, user-centered design, and individual coping mechanisms in managing technostress, especially in regions with high prevalence rates. In addition to these interventions, the review emphasizes the need for ongoing education and awareness campaigns to promote digital literacy and healthy technology habits. By equipping individuals with the knowledge and skills to navigate the complexities of the digital landscape, these initiatives can empower them to make informed decisions and effectively manage technostress in their daily lives. Highlighting the significance of culturally-tailored interventions, future research aims to refine mitigation strategies and investigate the potential benefits of technology use, ultimately fostering a balanced relationship with technology to minimize drawbacks and maximize advantages.

Current concepts in the diagnosis and management of acute pain in children

BACKGROUND: In children, injuries are the focus of attention both for the increasing incidence and necessity of pain management, and pain can be indicate the severity of injuries or serious complications that worsens the treatment results. Despite the obvious problem, information available in the literature regarding the theoretical and practical aspects of acute pain in children is often contradictory and needs to be systematized.&#x0D; AIM: To present current information about the epidemiology, physiology, diagnosis, and treatment of acute pain in children to pediatric orthopedic and trauma specialists.&#x0D; MATERIALS AND METHODS: Selective analysis and narrative review of relevant studies analyzing the epidemiology, diagnosis, and management of acute pain in children were performed.&#x0D; RESULTS: Although various pain assessment tools are available, the clinical assessment of acute pain in children remains challenging. The use of these tools depends on the child’s age, cognitive and communication skills, and pain location. The term oligoanalgesia has been used to describe inadequate pain relief in the emergency department. Oligoanalgesia in children has negative physiological and psychological effects, sometimes with long-term consequences, and may negatively affect their future pain experiences. Parents often underestimate their child’s pain level and have serious misunderstandings about how children express pain. The World Health Organization has developed recommendations for pain management in children. Ibuprofen and paracetamol are recommended as analgesics for mild-to-moderate pain in children aged 3 months. This choice considered extensive data on the effectiveness and safety.&#x0D; CONCLUSIONS: Personalized management strategies utilizing biopsychosocial approach will ensure that children are treated comprehensively according to their unique pain status.

Why Do Individuals Engage in Phubbing? An Empirical Study Integrating Fear of Missing Out, Boredom Proneness, Smartphone Use

Increased use of smartphones has necessitated research into its negative psychological and physiological effects on individuals. This study seeks to examine the relationship between psychosocial factors-boredom proneness, fear of missing out (FoMO) and life satisfaction with phubbing. The research also investigates the mediating effect of Phubbing on FoMO, boredom proneness and life satisfaction.&#x0D; Design/Methodology/Approach- A conceptual model is proposed and validated by extending research on smartphone use. A survey of 350 respondents was conducted using purposive sampling technique to empirically test the hypothesized linkages among the constructs using structural equation modeling.&#x0D; Findings- Results of the study confirmed that individuals who engage in phubbing behavior exhibit traits of boredom and FoMO. Findings also reveal that phubbing has a positive effect on life satisfaction. Additionally, results confirm that phubbing mediates the association between FoMO and life satisfaction.&#x0D; Limitations- The sample consisted of only students enrolled at a large, public university in India. While research suggests that students are the largest users of smartphones, and that smartphones are an important aspect of this generation’s culture and identity, our ability to generalize these results to other populations is limited and therefore replicating this study among different demographic and geographic regions will aid in generalizing the findings of the present study.&#x0D; Practical Implications- Findings of this study enrich the literature on smartphone use and reveals that phubbing is a direct consequence of individuals’ desire to gratify social and psychological needs. Outcome of the study also benefit practitioners by allowing them to understand and develop new interventions aimed at the youth so that they can be protected against the negative effects of smartphone use.&#x0D; Originality/Value- The present study attempts to analyze the effect of FoMO and boredom proneness on phubbing. Understanding the predictors of phubbing is important, considering the negative effects of this behavior on life satisfaction. This study makes significant contributions to the existing body of knowledge about how increased fear of missing out, boredom proneness and phubbing may affect overall life satisfaction.

The protective role of mindfulness in the cyberhate experience

In today's world, widespread and uncontrolled access to social media channels, along with the possibility for individuals to hide their identities or use pseudonyms, can turn the internet, especially the social media environment, into a tool for violence for women due to many potential situations. The purpose of this article is to inform about a study conducted with the participation of ten women actively involved in the Kars/Sarıkamış cooperative, focusing on the impact of mindfulness method on coping with the various short and long-term negative effects of cyberviolence on women. The research flow includes introducing the concepts discussed in the study and their relationships, the purpose of the research, the methodology, results, and future steps that can be taken. The aim of the research on the role of mindfulness in the experience of cyberviolence is to understand the effects of cyberviolence on women from the perspective of ten women participants actively involved in the Kars/Sarıkamış women's cooperative and the impact of mindfulness on women in coping with these effects. This study is of a qualitative nature, and survey research and focus group discussions have been preferred as the methods. The research results show that participants acknowledged the short and long-term negative psychological, physiological, and social effects of cyberviolence on individuals, but it also emerged that by strengthening solidarity among women and increasing the availability of methods such as mindfulness, the negative effects of cyberviolence can be reduced.

Body Positions Alter Hemodynamics and Respiration in Healthy Adults: A Systematic Review and Meta-Analysis

Objective Different body positions can exert both positive and negative physiological effects on hemodynamics and respiration. This study aims to conduct a literature review and examine hemodynamic and respiratory alterations to different body positions.Methods The study protocol was registered with the International Prospective Registry of Systematic Reviews (register no. CRD42021291464). Two independent reviewers evaluated the methodological quality of all included studies using the Down and Black checklist, while the quality of evidence was evaluated using the Grading of Recommendations, Assessment, Development, and Evaluations approach. The overall effects of different body positions were reported from random effects meta-analysis.Results Three studies with low risk of bias and ten with high risk of bias met the eligibility criteria. The supine resulted in the highest cardiac output compared to the 70 deg head-up tilt, sitting, and standing positions (very low- to moderate-quality evidences) and the lowest systemic vascular resistance compared to the 70 deg head-up tilt and standing positions (moderate-quality evidence). Additionally, the supine was associated with the highest total respiratory resistance compared to the 70 deg head-up tilt, left lateral, and standing positions (very low-to moderate-quality evidence) and higher alveolar ventilation than the prone (low-quality evidence).Conclusions The supine position has the most positive association with hemodynamic variables, resulting in the highest cardiac output and the lowest systemic vascular resistance. The upright positions (70 deg head-up tilt and standing positions) has the most positive association with the respiratory variables, resulting in the lowest total respiratory resistance.

Health and environmental effects to wildlife from radio telemetry and tracking devices-state of the science and best management practices.

This paper discusses the potential health risks and benefits to tagged wildlife from the use of radio tracking, radio telemetry, and related microchip and data-logger technologies used to study, monitor and track mostly wildlife in their native habitats. Domestic pets, especially canids, are briefly discussed as radio-tagging devices are also used on/in them. Radio tracking uses very high frequency (VHF), ultra-high frequency (UHF), and global positioning system (GPS) technologies, including via satellites where platform terminal transmitters (PTTs) are used, as well as geo-locating capabilities using satellites, radio-frequency identification (RFID) chips, and passive integrated responder (PIT) tags, among others. Such tracking technologies have resulted in cutting-edge findings worldwide that have served to protect and better understand the behaviors of myriad wildlife species. As a result, scientists, field researchers, technicians, fish and wildlife biologists and managers, plus wildlife and other veterinarian specialists, frequently opt for its use without fully understanding the ramifications to target species and their behaviors. These include negative physiological effects from electromagnetic fields (EMF) to which many nonhuman species are exquisitely sensitive, as well as direct placement/use-attachment impacts from radio collars, transmitters, and implants themselves. This paper provides pertinent studies, suggests best management practices, and compares technologies currently available to those considering and/or using such technologies. The primary focus is on the health and environmental risk/benefit decisions that should come into play, including ethical considerations, along with recommendations for more caution in the wildlife and veterinarian communities before such technologies are used in the first place.

From consumption to withdrawal: A broad synopsis of the effects of alcohol

AbstractThe consumption of alcohol reflects both societal norms and personal choices and has major clinical implications. Long‐term excess alcohol consumption leads to a domino effect of pathological outcomes and onset of chronic illness. However, the risks of alcohol consumption extend beyond long‐term health issues. Because habitual alcohol drinkers reach a state of systemic homeostasis, when they attempt withdrawal, they experience many negative physiological effects and may develop alcohol withdrawal syndrome. The complex interplay between alcohol consumption and withdrawal does not result solely in behavioral issues. Rather, a substantial number of important physiological adaptations and potential problems underly habitual drinking. Addressing these issues requires comprehensive medical observation and intervention. Here, we summarize the pathophysiological mechanisms, clinical diseases, and related treatment methods of alcohol consumption and withdrawal. The aim was to provide a reference to improve understanding of alcohol use and promote an informed approach to alcohol withdrawal.

Corticosterone and immune responses to dehydration in squamate reptiles.

Many environments present some degree of seasonal water limitations; organisms that live in such environments must be adapted to survive periods without permanent water access. Often this involves the ability to tolerate dehydration, which can have adverse physiological effects and is typically considered a physiological stressor. While having many functions, the hormone corticosterone (CORT) is often released in response to stressors, yet increasing plasma CORT while dehydrated could be considered maladaptive, especially for species that experience predictable bouts of dehydration and have related coping mechanisms. Elevating CORT could reduce immunocompetence and have other negative physiological effects. Thus, such species likely have CORT and immune responses adapted to experiencing seasonal droughts. We evaluated how dehydration affects CORT and immune function in eight squamate species that naturally experience varied water limitation. We tested whether hydric state affected plasma CORT concentrations and aspects of immunocompetence (lysis, agglutination, bacterial killing ability and white blood cell counts) differently among species based on how seasonally water limited they are and whether this is constrained by phylogeny. The species represented four familial pairs, with one species of each pair inhabiting environments with frequent access to water and one naturally experiencing extended periods (>30 days) with no access to standing water. The effects of dehydration on CORT and immunity varied among species. Increases in CORT were generally not associated with reduced immunocompetence, indicating CORT and immunity might be decoupled in some species. Interspecies variations in responses to dehydration were more clearly grouped by phylogeny than by habitat type.

Environmental Justice for Children of Color: Energy Facility Pollution in Houston

Extensive prior research has been conducted on the imbalance between environmental hazards in majority-minority communities and majority-white communities and its negative physiological, psychological, and economic effects. This existing body of literature has suggested a positive correlation between environmental hazards and poor childhood development indicators. The study of the field is called “environmental justice”, founded on the belief that certain groups are disproportionately disadvantaged by negative environmental conditions induced by inequitable laws and policies. However, a clear correlation between environmental racism and minority childhood experiences has not been studied. This paper utilizes a proximity analysis between Houston-area schools and toxin-emitting energy facilities and survey-based research with a school community affected by such facilities in southeast Houston. Through this research, a clearer picture can be drawn of how policy and inaction have put children of color at a structural disadvantage over their wealthier, whiter counterparts. Results have established that urban development policies, the placement of energy production facilities, a lack of equitable urban planning boards, disinvestment in infrastructure, and carelessness by government officials have contributed to environmental inequality, with children being at a heightened risk of its negative effects. After assessing the findings of the inquiry concerning the negative developmental impacts of environmental inequality on children, future ramifications are discussed concerning suggestions for possible solutions.

Responses of globally important phytoplankton species to olivine dissolution products and implications for carbon dioxide removal via ocean alkalinity enhancement

Abstract. Anthropogenic greenhouse gas emissions are leading to global temperature increases, ocean acidification, and significant ecosystem impacts. Given current emissions trajectories, the IPCC's reports indicate that rapid abatement of CO2 emissions and development of carbon dioxide removal (CDR) strategies are needed to address legacy and difficult-to-abate emissions sources. These CDR methods must efficiently and safely sequester gigatons of atmospheric CO2. Coastal enhanced weathering (CEW) via the addition of the common mineral olivine to coastal waters is one promising approach to enhance ocean alkalinity for large-scale CDR. As olivine weathers, it releases several biologically active dissolution products, including alkalinity, trace metals, and the nutrient silicate. Released trace metals can serve as micronutrients but may also be toxic at high concentrations to marine biota, including phytoplankton, which lie at the base of marine food webs. We grew six species representing several globally important phytoplankton species under elevated concentrations of olivine dissolution products via a synthetic olivine leachate (OL) based on olivine's elemental composition. We monitored their physiological and biogeochemical responses, which allowed us to determine physiological impacts and thresholds at elevated olivine leachate concentrations, in addition to individual effects of specific constituents. We found both positive and neutral responses but no evident toxic effects for two silicifying diatoms, a calcifying coccolithophore, and three cyanobacteria. In both single and competitive co-cultures, silicifiers and calcifiers benefited from olivine dissolution products like iron and silicate or enhanced alkalinity, respectively. The non-N2-fixing picocyanobacterium could use synthetic olivine-derived iron for growth, while N2-fixing cyanobacteria could not. However, other trace metals like nickel and cobalt supported cyanobacterial growth across both groups. Growth benefits to phytoplankton groups in situ will depend on species-specific responses and ambient concentrations of other required nutrients. Results suggest olivine dissolution products appear unlikely to cause negative physiological effects for any of the phytoplankton examined, even at high concentrations, and may support growth of particular taxa under some conditions. Future studies can shed light on long-term eco-evolutionary responses to olivine exposure and on the potential effects that marine microbes may in turn have on olivine dissolution rates and regional biogeochemistry.

A qualitative assessment of insomnia in recovering alcohol-dependent patients.

Insomnia is a common symptom that may interfere with the efforts of alcohol-dependent patients to initiate and maintain sobriety. This study investigates the experiences of recovering alcohol-dependent patients with insomnia as well as preferences for treatment strategies. Semi-structured interviews were conducted with 27 patients in Ukraine and Germany. Most of the patients experienced negative psychological (n = 21), physiological (n = 14) effects and negative daytime consequences during insomnia. All 27 patients were engaged in nonpharmacological approaches to insomnia and 52% used pharmacological approaches to manage insomnia. This study provides insight for understanding how alcohol-dependent patients in recovery perceive insomnia.

Contribution of Arbuscular Mycorrhizal Fungi (AMF) in Improving the Growth and Yield Performances of Flax (Linum usitatissimum L.) to Salinity Stress

Throughout the world, salinity is a major environmental issue that limits agricultural productivity, particularly in arid and semi-arid regions. In addition, climate change is the most important reason for the salinization of agricultural soils in the world, so it is now essential to find solutions to increase salinity tolerance in plants. This study investigated the potential of arbuscular mycorrhizal fungi (AMF) inoculation to enhance the growth and yield performances of flax under different salinity levels by conducting a pot experiment. The experiment was laid out in a two-factor completely randomized design including AMF inoculation (AMF+: with inoculation; AMF−: without inoculation) and irrigation water salinity (0, 50, 100, and 150 mM NaCl). According to the results, it is evident that salt stress caused negative physiological effects, including limited growth, reduced photosynthesis, and decreased nitrogen (N) and phosphorus (P) content in the shoots and roots of flax plants. Moreover, mycorrhizal association improved the salt tolerance of the plants by increasing chlorophyll content, and enhancing N and P shoot and root contents and consequently yield parameters, such as seed and stem fiber yield, particularly at moderate salt concentrations (50 and 100 mM NaCl). In particular, under 100 mM, AMF increased the total chlorophyll content, N shoot and root content, P shoot and root content, and seed and stem fiber yield by 30.4%, 36.1%, 31.0%, 38.9%, 45.4%, 35.2%, and 26.9%, respectively. As a result of using AMF, flax plants grown under salt stress exhibited tolerance, suggesting that AMF could be applied in saline environments to maintain ecological stability.

Effect of different air speeds at cow resting height in freestalls on heat stress responses and resting behavior in lactating cows in Wisconsin

Heat abatement (e.g., soakers, fans) effectively reduces the negative physiological and production effects of heat stress, but no previous studies have documented effective interventions for the reduced lying times observed in response to hot weather. Although likely adaptive for heat dissipation, the reduction in motivated lying behavior may be an animal welfare concern. We evaluated the effect of air speed from fans with variable frequency drives on the heat stress responses of cows in a naturally ventilated freestall barn. Eight groups of lactating Holsteins (16 cows/group) were exposed to 3 treatments in a replicated crossover design: control (fans off, 0.4 ± 0.2 m/s, measured 0.5 m above the stall surface to represent cow resting height) vs. 60% (1.7 ± 0.5 m/s; ≥ 1 m/s in all stalls) and 100% (2.4 ± 0.8 m/s) fan power. Each treatment was applied for 3 d of acclimation and 4 d of data collection. The effects of treatment on daily maximum vaginal temperature (VT) and lying time (LT; both measured with data loggers), respiration rate (RR; recorded from video), unshaved scapular skin temperature (ST), milk yield (MY), and dry matter intake (DMI) were analyzed using linear mixed models. All models included the fixed effect of treatment and a repeated term for treatment day within group of cows, with group as the subject. The models for LT, VT, and RR also included a fixed effect for same-day Temperature Humidity Index (THI; recorded in the pens with data loggers) and the THI × treatment interaction. The models for DMI and MY, using data from the latter 3 d of each treatment period, also included a fixed effect for the previous day's THI and the -1 d THI × treatment interaction. Lying time differed among treatments (100% vs. 60% fan power vs. control: 14.2 vs. 13.9 vs. 13.2 h/d, respectively, SEM = 0.15 h/d), but both fan treatments prevented the reduction in LT observed in the control treatment as THI increased. Relative to the control, both fan treatments effectively reduced ST, RR, and VT and increasing DMI and MY. In the control, average values were elevated for both RR (68.7 ± 1.5 breaths/min, mean ± SEM, greater than a common benchmark of 60 breaths/min) and VT (39.3 ± 0.05°C) but remained in the normal range in both fan treatments (54.2 vs. 50.7 breaths/min in the 60% vs. 100% fan power treatments; 39.0°C in both fan treatments). Both fan treatments resulted in greater overall MY (42.6 vs. 43.0 ± 0.4 kg/d in the 60% vs. 100% fan power treatments) relative to the control (41.0 kg/d) and similarly avoided the reduction in MY when -1 d THI increased. Compared with natural ventilation alone, fans delivering air speeds of at least 1 m/s at cow resting height were effective not only for reducing thermoregulatory responses, but also for maintaining lying time, DMI, and milk yield in heat stress conditions. This is the first study to demonstrate an intervention to improve animal welfare by maintaining lying times during periods of heat stress.

A mitotoxic fungicide alters post-ingestive glucose signals necessary for associative learning in honey bees

The Proboscis Extension Reflex (PER) paradigm trains honey bees to associate an odor with a sugar reward and is commonly used to assess impacts on associative learning after exposure to pesticides. While the effects of some types of pesticides have been well-investigated, relatively little attention has been focused on fungicides that are applied to flowering crops. We have previously shown that consumption of field-relevant concentrations of the fungicide Pristine® (active ingredients: 25.2% boscalid, 12.8% pyraclostrobin) impairs honey bee performance in an associative learning assay, but the mechanism of its action has not been investigated. We hypothesized that Pristine® interferes with carbohydrate absorption and/or regulation, thereby disrupting the post-ingestive feedback mechanisms necessary for robust learning. To test this hypothesis, we measured hemolymph glucose and trehalose levels at five time points during the ten minutes after bees consumed a sucrose solution. Pristine®-exposed bees had elevated baseline glucose concentrations in the hemolymph relative to control bees. Hemolymph glucose levels rose significantly within five minutes of feeding in control bees, but not in Pristine®-fed bees. These data suggest that the post-ingestive feedback mechanisms necessary for robust learning are disrupted in bees that have consumed this fungicide, providing a plausible mechanistic explanation for its effects on learning performance in the PER assay. Pristine®-exposed bees may have elevated hemolymph glucose levels because the fungicide elicits an inflammatory response. These results provide additional mechanistic understanding of the negative physiological effects of mitotoxic fungicides on this important pollinator.

Negative psychological and physiological effects of social networking site use: The example of Facebook.

Social networking sites (SNS), with Facebook as a prominent example, have become an integral part of our daily lives and more than four billion people worldwide use SNS. However, the (over-)use of SNS also poses both psychological and physiological risks. In the present article, we review the scientific literature on the risk of Facebook (over-)use. Addressing this topic is critical because evidence indicates the development of problematic Facebook use ("Facebook addiction") due to excessive and uncontrolled use behavior with various psychological and physiological effects. We conducted a review to examine the scope, range, and nature of prior empirical research on the negative psychological and physiological effects of Facebook use. Our literature search process revealed a total of 232 papers showing that Facebook use is associated with eight major psychological effects (perceived anxiety, perceived depression, perceived loneliness, perceived eating disorders, perceived self-esteem, perceived life satisfaction, perceived insomnia, and perceived stress) and three physiological effects (physiological stress, human brain alteration, and affective experience state). The review also describes how Facebook use is associated with these effects and provides additional details on the reviewed literature, including research design, sample, age, and measures. Please note that the term "Facebook use" represents an umbrella term in the present work, and in the respective sections it will be made clear what kind of Facebook use is associated with a myriad of investigated psychological variables. Overall, findings indicate that certain kinds of Facebook use may come along with significant risks, both psychologically and physiologically. Based on our review, we also identify potential avenues for future research.

Racial Identity and Mindfulness as Predictors of Posttraumatic Growth in Black Adults Experiencing Race-Based Trauma.

Racial discrimination and race-based trauma (RBT) have led to the development of various negative psychological and physiological effects among Black adults in the USA. There is a lack of understanding in relation to how various psychosocial factors influence posttraumatic growth (PTG) in the context of RBT among Black adults. The authorsexamined associations of RBT, racial identity, and mindfulness with PTG among Black adults while controlling for gender, household income, and duration of trauma. The sample consisted of 134 self-identified Black adults who met the criteria for RBT from the USA. The hierarchical regression analysis showed the final model with all the predictors accounted for 35% of the total variance of PTG, with racial identity and mindfulness facets accounting for 26% of the variance. The study provides a foundation for future research addressing RBT and promoting PTG in Black adults.

Reproductive toxicity of polystyrene nanoplastics in Drosophila melanogaster under multi-generational exposure

Micro-nanoplastics have become a new type of pollutant worldwide and have attracted widespread attention for their potential toxicity. However, the toxicity of polystyrene nanoplastics (PS-NPs) under continuous exposure of multi-generations is still unclear. In the present study, Drosophila melanogaster was selected as an in vivo biological model to investigate the reproductive toxicity and underlying mechanism induced by PS-NPs (100 nm; 1, 10, 50, and 100 mg L−1) after continuous exposure of five generations. The results showed that PS-NPs accumulated in the crop, gut and ovaries after 5 d of exposure. It was also observed that the number of egg production and eclosion rate decreased significantly (P < 0.05) accompanied by delayed development during continuous exposure PS-NPs of multi-generations. Further analysis revealed that the degree of apoptosis and necrosis of oocytes in the F5 generation increased with the increasing exposure dose. To elucidate the underlying toxicity mechanism mediated by PS-NPs, transcriptomic analysis was performed on the ovaries of the F5 generation. The results showed that there were 102 and 208 differentially expressed genes (DEGs) in the 1 mg L−1 and 100 mg L−1 PS-NPs treatment groups, respectively, compared with the control group. The transcriptome analysis further detected the KEGG pathway with significant enrichment of DEGs, revealing obvious reproductive toxicity at the molecular level. In conclusion, this research not only highlighted the negative physiological effects of multi-generational exposure to PS-NPs on Drosophila melanogaster, but also explored potential mechanisms by transcriptomic analysis to better understand reproductive toxicity induced by multi-generational exposure.

Effects of OmniGen AF feed supplementation on glucocorticoids, blood leukocyte messenger RNA abundance, and energy metabolism in overstocked Holstein dairy cows

The physiological consequences of overstocking require more investigation, and no research has explored whether dietary supplements could mitigate the anticipated negative physiological effects. OmniGen AF (OG, Phibro Animal Health Corporation, Teaneck, NJ, USA) is a nutritional supplement that has been shown to support the immune system of cattle following internal and environmental stressors. This study aimed to determine if a 45-day period of OG feed supplementation would influence whole blood leukocyte messenger RNA abundance, energy metabolism and glucocorticoid concentration, during a two-week period of overstocking. Two stocking density treatments (control: one headlock and lying stall per cow; overstocked: 0.5 headlocks and 0.5 lying stalls per cow) and two diet treatments (control: no added supplement; and OG: 56 g/cow per day) were investigated. Four pens of 15 cows were fed their assigned diet (two pens per diet; control stocking density) for 45 days after which each stocking density treatment was applied for a 14-day period using a cross-over design; this study design was replicated twice. During each 14-day period, blood was collected on day four to measure whole blood leukocyte messenger RNA abundance (cluster of differentiation 80, interleukin 8 receptor-beta, interleukin 10 receptor-beta and L-selectin) and fecal samples were collected every two days to measure fecal cortisol metabolite concentration (11,17-dioxoandrostanes). At the end of each 14-day period, eight cows from each pen were selected for an intravenous glucose tolerance test; glucose, insulin and non-esterified fatty acids were measured. There were no effects of diet or stocking density on leukocyte messenger RNA abundance. Fecal cortisol metabolite concentrations were highest for overstocked cows on the control diet on day four of the stocking density treatment; however, by day 10, overstocked cows fed OG had the highest fecal cortisol metabolite concentrations. Overstocked cows, regardless of diet, had an attenuated insulin response during the glucose tolerance test, represented by a lower area under the curve estimate. Cows fed OG but not overstocked, had a lower non-esterified fatty acid nadir during the glucose challenge, compared to all the other treatments. In conclusion, overstocking prompts a physiological stress response and alters energy metabolism by decreasing the insulin response to an intravenous glucose challenge. Feeding OG during overstocking delayed the increase in fecal cortisol metabolites by several days; however, it is unclear if this altered glucocorticoid response benefited the cow, as OG had no effect on insulin responses or immune parameters.