Need For Secondary Surgery Research Articles

Outcomes and Predictive Factors of Furlow Palatoplasty

Objective: 1) Describe patients with submucous cleft palates (SMCP) and velopharyngeal insufficiency (VPI) treated with furlow palatoplasty who later required subsequent surgery due to persistent VPI. 2) Identify risk factors predictive of revision surgery in SMCP patients in order to develop a clinical algorithm to apriori determine the most efficacious primary surgery based on preoperative evaluation. Method: A retrospective review of 14 patients with SMCP undergoing furlow palatoplasty to correct VPI. Data reviewed included need for secondary surgery, syndrome diagnosis, velopharyngeal closure, and preoperative speech evaluations. Results: A series of 14 patients having furlow palatoplasty between ages 3 and 16 (average age of 4.9 years) was identified. Three of these patients (21.4 %) required secondary correction of VPI via superiorly based pharyngeal flap an average of 2.3 years following the initial operation. There was no association between age at primary surgery or syndromic diagnosis and persistent VPI. Predictors of persistent VPI after palatoplasty were poor preoperative speech ( P < .03) and nasometry scores ( P < .09). Pattern of velopharyngeal closure was not predictive of further surgery, however an association between larger velopharyngeal gap and need for revision surgery was found. Conclusion: Preoperative speech and nasal endoscopic assessment of patients with SMCP and VPI is predictive of patients who require palatoplasty alone or may benefit from primary velopharyngeal surgery.

Posttraumatic Reconstruction of the Hand—A Retrospective Review of 87 Toe-to-Hand Transfers Compared With an Earlier Report

The purpose of this study was to retrospectively review the results of 87 toe-to-hand transfers performed in 73 procedures, compare them to the report published by the senior author in 1983, and confirm the hypothesis that results of toe-to-hand transfers at our center have improved over time. The results of 87 toe-to-hand transfers performed between 1981 and 2001 were reviewed and compared with the results of 54 toe-to-hand transfers performed between 1974 and 1980. The measured parameters were type of reconstruction performed, anticoagulation therapy, vascular patency, frequency of secondary surgery, and strength of thumb reconstructions. In the recent time period, 11% of the procedures had complications with revascularization of the transferred digit, and long-term survival was seen in 98% of the toe-to-hand transfers. This is a significant improvement over earlier results, in which 33% of the cases had some microvascular compromise and the survival of grafts was lower (91%). Pinch strength for thumb reconstructions improved, and the number of secondary surgeries performed dropped, but neither of these parameters reached a significant level. Toes used for reconstruction changed, with an 18% decrease in use of big toe for thumb reconstruction and a similar increase in use of the second toe. For non-thumb digital reconstructions there was a 60% decrease in use of second and third toe combined, whereas use of the second toe alone increased similarly. This study showed reduction of the incidence of vascular compromise compared to the previous report. Improved strength of thumb reconstructions and reduced need for secondary surgery was also displayed. These findings are likely attributed to refinements in reconstructive procedures and operative techniques. Therapeutic IV.

Posterior screw fixation in rotationally unstable pelvic ring injuries

Objective Although the stability of the pelvic ring primarily depends on the integrity of the posterior sacroiliac arch, lateral compression fractures with rotational instability are commonly treated by anterior fixation alone. The objective of the present study was to assess the outcome of patients with these fractures treated by posterior iliosacral screw fixation alone. Methods Patients with rotationally unstable lateral compression fractures of the pelvic ring (Young and Burgess LC I and LC II or AO/Tile B2) treated by percutaneous iliosacral fixation alone were included. Postoperative complications, need for secondary surgery, malunion, secondary fracture displacement and the time to full-weight bearing were documented. Results Twenty-five patients (13 female, 26 male; age: 56 ± 20 years) were treated by percutaneous screw fixation (14 bilaterally, 11 unilaterally). Mean follow-up was 6 ± 4 months, mean time to full weight bearing 9 ± 3 weeks. Revision surgery was necessary in two patients (8%) due to nerve irritation; an additional anterior stabilisation was needed in two other patients (8%) due to secondary dislocation. Wound infection or motor weakness were not encountered, non-union of the posterior arch did not occur. Non-union of the pubic rami, however, occurred in two patients. The presence of malunion of the pubic rami did not affect the time to full weight bearing. Conclusions Percutanous iliosacral screw fixation alone is a sufficient technique for the stabilisation of rotationally unstable pelvic fractures with low rates of complications or non-unions. It allows for a minimally invasive treatment thus being a useful option in patients who do not qualify for open anterior fixation.

Short-Term and Long-Term Effects of Orthopedic Biodegradable Implants

Presently, orthopedic and oral/maxillofacial implants represent a combined $2.8 billion market, a figure expected to experience significant and continued growth. Although traditional permanent implants have been proved clinically efficacious, they are also associated with several drawbacks, including secondary revision and removal surgeries. Non-permanent, biodegradable implants offer a promising alternative for patients, as they provide temporary support and degrade at a rate matching tissue formation, and thus, eliminate the need for secondary surgeries. These implants have been in clinical use for nearly 25 years, competing directly with, or maybe even exceeding, the performance of permanent implants. The initial implantation of biodegradable materials, as with permanent materials, mounts an acute host inflammatory response. Over time, the implant degradation profile and possible degradation product toxicity mediate long-term biodegradable implant-induced inflammation. However, unlike permanent implants, this inflammation is likely to cease once the material disappears. Implant-mediated inflammation is a critical determinant for implant success. Thus, for the development of a proactive biodegradable implant that has the ability to promote optimal bone regeneration and minimal detrimental inflammation, a thorough understanding of short- and long-term inflammatory events is required. Here, we discuss an array of biodegradable orthopedic implants, their associated short- and long- term inflammatory effects, and methods to mediate these inflammatory events.

An all-endo Approach to Complete Ureteral Duplications Complicated by Ureterocele and/or Vesicoureteral Reflux: Feasibility, Limitations, and Results

Purpose. Totally endoscopic management (all-endo) of patients with a duplicated renal system (DS) associated with severe vesicoureteral reflux (VUR) or obstructive ureterocele (UC) is an attractive alternative to traditional open procedures. The authors discuss feasibility and results of an all-endo approach on a consecutive series of patients. Methods. From 1999 to 2009, all patients with a complete DS associated with UC and/or VUR were proposed for primary all-endo approach. UC puncture was performed using a 3 Fr Bugbee electrode. Deflux (dextranomer/hyaluronic acid copolymer) injection was administered for VUR. The need for secondary surgery was evaluated on followup. Results. Of the 62 patients recruited, 46 were treated using a primary all-endo approach and 16 patients received no treatment. Of the 46 treated patients with 56 affected renal units, 32 (97%) UCs collapsed following puncture and 29 (63%) VURs were resolved or downgraded. Secondary VUR occurred in 13 (39%) renal units. Secondary surgery was performed on 23 (41%) renal units. Conclusion. The all-endo approach for VUR in DS is an effective therapeutic option. UC collapse was achieved by puncture in most of the patients; secondary VUR was the main complication in a small group of extravesical UC.

Percutaneous iliosacral screw fixation in S1 and S2 for posterior pelvic ring injuries: technique and perioperative complications

Percutaneous iliosacral screw placement allows for minimally invasive fixation of posterior pelvic ring instabilities. The objective of this study was to describe the technique for screws in S1 and S2 using conventional C-arm and to evaluate perioperative complications. Thirty-eight consecutive patients after percutaneous pelvic ring fixation with cannulated screws in S1 and S2 using conventional C-arm fluoroscopy were analysed. Accuracy of screw placement, nerval lesions, need for second surgery, operation time, and time to full weight bearing were assessed postoperatively and during regular follow-up examinations. Twenty-one patients underwent unilateral screw fixation and 17 patients underwent bilateral screw fixation. In total, 83 screws were placed. Mean age of the patients was 52 ± 19 years. Mean operation time was 16 ± 7 min/screw. Mean follow-up was 5 ± 3 months. Time to full weight bearing in 28 patients was 9 ± 4 weeks. Eight patients were still not able to support full weight bearing, partially due to concomitant injuries. Patients without concomitant injuries that affected walking were able to bear full weight after 8 ± 4 weeks (n = 17). Two patients had persistent postoperative hypaesthesia. No motor weakness was apparent and no postoperative bleeding was observed. Secondary surgery due to screw malpositioning or loosening had to be performed in four patients. The presence of a screw in S2 was not indicated for perioperative complications. Percutaneous iliosacral screw fixation is a rapid and definitive treatment for posterior pelvic ring injuries with a low risk of secondary bleeding during posterior pelvic stabilization. The technique using standard C-arm fluoroscopy was also found to be safe for screws placed in S2.

Treatment of Acetabular Fractures in an Older Population

To identify factors predicting poor radiographic and functional outcome and delayed total hip arthroplasty in operatively managed acetabular fractures in patients 55 years of age and older. Retrospective chart and radiographic review of a prospectively maintained database. Tertiary care hospital. Ninety-three with a mean age of 67 years met all inclusion criteria and had follow up averaging 5 years. Open reduction and internal fixation and less commonly acute total hip arthroplasty for displaced acetabular fractures in an older cohort. Three validated patient self-assessment measures were used: the Musculoskeletal Functional Assessment, the Short Musculoskeletal Functional Assessment, and the SF-36. The overall rate of hip replacement in our study was 30.95%. Poor fracture reduction (P < 0.02), development of avascular necrosis (P < 0.001), and previous contralateral hip arthroplasty (P = 0.02) were statistically associated with the need for secondary surgeries. Functional outcome scores in the current study compared favorably with functional outcome scores reported for acetabular fractures in younger populations and with age-matched "non-injured" norms published in recent literature. There was an acceptably low rate of major complications in 93 operatively managed fractures in this population. Nearly 70% of patients achieved functional outcomes similar to age- and injury-matched control subjects without the need for secondary surgeries. Thirty percent of patients required secondary total hip arthroplasty for posttraumatic arthritis. These patients achieved outcomes similar to patients in the other outcome groups and to injury- and age-matched norms.

Late Reduction in Congenital Dislocation of the Hip and the Need for Secondary Surgery

Despite early recognition and appropriate treatment of congenital dislocation of the hip, there are a number of cases that subsequently require further surgery to prevent progressive dysplasia, instability, and eventual early osteoarthritis. This study aimed (1) to determine the incidence of pelvic osteotomy (PO) after late open (OR) or closed (CR) reduction for failed initial conservative treatment or late presentation; (2) study potential radiologic predictors of those that will require a secondary procedure; (3) and to evaluate the effect of potential confounding variables including age of reduction, Pavlik harness treatment, and surgical experience on PO rate. All cases of congenital dislocation of the hip that presented late or had failed conservative treatment with subsequent late OR versus CR, that were carried out during 1988 to 2003, by the lead surgeon were included. Dislocations secondary to neuromuscular causes or teratologic causes were excluded. Intraoperative arthrograms confirmed the concentric or eccentric reduction and determined subsequent intervention. The AP pelvis plain radiograph was used to measure the height of dislocation, as described by Tonnis, and monitor Acetabular index, and ossific nucleus width and height postreduction. After 134 OR's, 24 hips (19%, 95% CI: 16-23%) later required a pelvic osteotomy compared with 59 out of 104 hips (58%, 95% CI: 49-68%) in the CR cohort. There was no statistical difference in avascular necrosis rates between late OR (10.9%, 95% CI: 4.8-17%) and CR (11.4%, 95% CI: 5.8-17%). Acetabular index was a reliable predictor for the need of subsequent PO becoming significantly different in those that did (PO group) and did not (non-PO group) require further surgery approximately 1.5 years postreduction. There was no difference in the ON development after reduction in both PO and non-PO groups. The PO requirement was not affected by earlier failed Pavlik harness treatment but did change with ongoing surgical experience. Late OR produced the lowest secondary procedure rate without an increase in the incidence of avascular necrosis. There is a learning curve to this procedure that will affect these outcomes. Level III (Case-control study).

Selected Outcomes of Thumb Replantation After Isolated Thumb Amputation Injury

The aim of this study was to assess thumb survival, pinch strength, grip strength, and need for secondary surgery in patients undergoing thumb replantation after isolated thumb amputation injury. We conducted a retrospective review of 52 consecutive isolated thumb replantations performed over a 4.5-year period. Charts were reviewed for mechanism of injury, level of amputation, and surgical technique. Primary outcomes of interest included survival and secondary surgery (eg, tenolysis, neurolysis) rates. Functional outcome was assessed by pinch and grip strengths after a mean follow-up period of 10 months from the initial injury. The overall thumb survival rate was 92% (48 of 52). One hundred percent of Zone I injuries (13 of 13), 94% of zone II injuries (29 of 31), and 75% of zone III injuries (6 of 8) survived; overall survival was 94% in sharp injuries (32 of 34), 89% in avulsion injuries (8 of 9), and 89% in crush injuries (8 of 9). Secondary surgery was performed in 18 patients with increasing need across the 3 zones (0%, 42%, and 63%, respectively; p for trend = .002). Pinch and grip strengths of 17 patients after an average follow-up period of 10 months were significantly worse after crush/avulsion injuries (p = .007 and .07, respectively) and injuries requiring joint intervention (p = .004 and .02, respectively); grip strength was also found to be negatively associated with increasing zone of injury. This retrospective study shows that a high rate of survival can be achieved after thumb replantation using current techniques. In addition, the need for secondary surgery is strongly related to zone of injury, with zone I injuries requiring the least amount of secondary surgery. Finally, pinch and grip strengths may be worse after crush or avulsion injuries and injuries requiring joint intervention. Therapeutic IV.

Utility of the GeneSearch breast lymph node assay for the rapid evaluation of sentinel lymph nodes in breast cancer

The potential for reducing the need for second surgery for axillary lymph node dissection (ALND) has made the intraoperative evaluation of sentinel lymph nodes (SLNs) attractive. The goal of the current study was to evaluate the clinical application of the breast lymph node (BLN) assay, a real-time reverse transcriptase-polymerase chain reaction assay for SLN metastases, by comparing this test with routine pathologic examination. A total of 117 patients with breast cancer underwent breast surgery with SLN biopsy. Each SLN was cut in half along the plane of the longest dimension. Half of each lymph node was examined by the 2 markers of the BLN assay, mammaglobin and cytokeratin 19, and the other half was examined by hematoxylin and eosin staining (H&E) and immunohistochemical staining (IHC) for pancytokeratins. A total of 204 SLNs were obtained from 117 patients. H&E staining identified metastases in 31 SLNs (15.2%), and IHC staining detected metastases in 6 SLNs; 40 SLNs from 32 patients were found to be positive for metastasis using the BLN assay. The assay results were correlated with the pathologic diagnoses by H&E and IHC staining (P<.001). The sensitivity of the BLN assay compared with pathologic findings classified according to the TNM classification was 95.7% for macrometastases, 60.0% for micrometastases, and 55.6% for isolated tumor cells. The 2-marker BLN assay performs in a manner that is comparable to, and analyzes more tissue than, routine pathologic examination. Therefore, clinical intraoperative use of the BLN assay for SLNs may result in a reduction in the need for second surgery for ALND.

Decal bone matrix as a local antibiotic delivery vehicle in a MRSA-infected bone model: An experimental study

Background:Polymethyl methacrylate (PMMA) antibiotic beads though have proved their utility as a local antibiotic delivery system, however, there are limitations. Decalcified bone matrix (DBM) as a vehicle of antibiotics can serve the purpose, provided a minimum inhibitory concentration is sustained. Healing of the defect and avoiding the second surgery is another advantage. We studied the DBM as the delivery vehicle for vancomycin in controlling the methicillin-resistant Staphylococcus aureus (MRSA) osteomyelitis as well as healing of the cavity simultaneously in an experimental study.Materials and Methods:An in vitro study was conducted to optimize vancomycin impregnation in the DBM. For the in vivo study, a unicortical defect was created in the metaphysis of the distal femur in 18 rabbits. After contaminating the defect with MRSA, rabbits were divided into three groups. Group I (eight limbs) received no graft. Defects in group II (11 limbs) were filled with plain DBM chips and in group III (14 limbs), cavities were implanted with vancomycin-impregnated decal bone chips. Rabbits were assessed by clinical, radiological, histological, gross examination and bacterial load assay. High Performance Liquid Chromatography HPLC analysis of vancomycin in group III was done to assess the concentration in DBM chips.Results:In group I, the infection persisted throughout the period of the study. Group II showed the fulminated infection at the grafted site with DBM chips sequestrating out. Vancomycin-impregnated decal chips in group III did not show any sign of infection and eventually incorporated. The bacterial load study showed a progressive load change and HPLC revealed an effective antibiotic concentration up to 3 weeks in both in vitro and in vivo.Conclusion:Decal bone chips were effective as the local antibiotic delivery vehicle in preventing the MRSA osteomyelitis model. It eluted vancomycin significantly and the graft uptake was also excellent. Allogeneic decal grafts eliminated the need for second surgery and acted as an excellent delivery vehicle for antibiotics.

2107 FOCUSED ULTRASOUND APPLICATION TO REPOSITION KIDNEY STONES AND FACILITATE PASSAGE OF RESIDUAL FRAGMENTS

You have accessJournal of UrologyStone Disease: New Technology1 Apr 20102107 FOCUSED ULTRASOUND APPLICATION TO REPOSITION KIDNEY STONES AND FACILITATE PASSAGE OF RESIDUAL FRAGMENTS Anup Shah, Mathew Sorensen, Jonathan Harper, Bryan Cunitz, Wei Lu, and Michael Bailey Anup ShahAnup Shah More articles by this author , Mathew SorensenMathew Sorensen More articles by this author , Jonathan HarperJonathan Harper More articles by this author , Bryan CunitzBryan Cunitz More articles by this author , Wei LuWei Lu More articles by this author , and Michael BaileyMichael Bailey More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2010.02.2194AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Lower pole kidney stones have lower rates of stone clearance after shock wave lithotripsy (SWL) and ureteroscopic treatment compared to other stone locations because of dependant positioning and difficult access. Residual stone fragments often lead to additional morbidity and need for secondary surgery. We describe the use of acoustic force and streaming created by transcutaneous focused ultrasound to manipulate the location of stone fragments within the collecting system in order to facilitate stone clearance. METHODS A newly developed focused ultrasound therapy probe consists of an 8-element annular array with a nominal frequency of 2.0 MHz, acoustic power output of 5-20W, and programmable focal depth of 4.5-8.5 cm. There is an opening in the center of the therapy probe for an HDI P4-2 imaging probe to provide ultrasound image guidance in a 2D plane. Artificial and human stones were placed into the lower pole of transparent kidney phantoms and live porcine kidneys using retrograde ureteroscopy. Focused ultrasound was applied. Stone motion was observed visually in the kidney phantom and using both diagnostic ultrasound and fluoroscopy in the porcine kidneys. Any tissue effects caused by focused ultrasound were assessed. RESULTS All stones placed in the kidney phantoms and live porcine kidneys were seen to move. Stone size ranged from 2 mm to 6 mm in maximal dimension. Stone velocities were on the order of 1 cm/s. Stone displacement distance was up to 3 cm and operators could generally control the direction of stone movement. No evidence of thermal necrosis or mechanical damage of renal tissue was observed on gross and microscopic examination. CONCLUSIONS Focused ultrasound can be used to move stones within the collecting system and may be able to improve rates of lower pole stone fragment clearance. Other applications for this new technology may include adjunctive use with medical expulsive therapy, repositioning of obstructing ureteropelvic junction stones, and ensuring complete clearance of infectious stones. Seattle, WA© 2010 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 183Issue 4SApril 2010Page: e819 Advertisement Copyright & Permissions© 2010 by American Urological Association Education and Research, Inc.MetricsAuthor Information Anup Shah More articles by this author Mathew Sorensen More articles by this author Jonathan Harper More articles by this author Bryan Cunitz More articles by this author Wei Lu More articles by this author Michael Bailey More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...

Marknagelung nach Aufbohrung

The stabilization of long bone fractures through reaming the medullary cavity and insertion of an intramedullary nail, with or without locking bolts, was a standard procedure in fracture treatment for a long time. With the development of nails, which can be applied without reaming, the procedure lost importance. This publication presents the mechanical principles and impact of reaming on the bone. It describes how the pressure of the drill head and drill wave leads to a compression of medullary contents into the cortical vascular system. With the development of new drilling devices the direct effects of pressurization can be minimized and then become similar to those of unreamed intramedullary nailing. Lowering the pressure during reaming also prevents the formation of mixed thrombosis on the base of a nucleus of the bone marrow in the venous circulatory system and the danger of a pulmonary embolism. The majority of experimental research shows slightly faster fracture healing after reamed nailing. In the few randomized clinical studies presented, exclusively coming from Canada, accelerated fracture healing has also been confirmed. Aside from this it was also shown that there were fewer cases of non-unions and implant failures and less need for secondary surgeries when reamed nailing was used. Despite the difficulty in rating the clinical trials presented, due to the high number of participating clinics, the impression remains that in the course of fracture healing intramedullary reamed nailing has some advantages in comparison to unreamed nailing. An obvious advantage is that earlier and sometimes immediate weight-bearing can be tolerated. Today nevertheless unreamed nailing is favoured especially in German-speaking countries. This is maybe due to the necessary classic procedure of planning, positioning on the fracture table and reaming in steps, which make the operation slightly more complex, requiring a certain amount of armamentarium and technical understanding. In any case reamed intramedullary nailing offers an alternative procedure for many indications; to treat a hypertrophic non-union of the shaft of the long bones it remains the method of first choice.

Evaluation of the histological size of the sentinel lymph node metastases using RT–PCR assay: a rapid tool to estimate the risk of non-sentinel lymph node invasion in patients with breast cancer

A RT-PCR assay (GeneSearch™, Veridex, LLC), FDA approved and CE marked to detect metastases > 0.2 mm in sentinel lymph nodes (SLNs) is used intra-operatively for the management of patients with breast cancer. The assay provides qualitative results by applying cut-off values to cycle times (Ct) for mammaglobin (MG) and cytokeratin-19 (CK19) genes. Aims of this study were to evaluate the performance of the quantitative Ct values to estimate the size of nodal metastases and the risk of additional disease in non-SLNs. SLNs from 367 patients were clinically processed using both BLN assay and post-operative histology. Complementary axillary lymph node dissection (ALND) was performed concurrently in case of BLN assay positivity or tumour size > 2 cm. BLN positivity was reported in 19.6% of the patients for a sensitivity of 89%. BLN specificity (94.5%) and negative predictive value (97.5%) clearly demonstrated its reliability to guide ALND decision. All, except one, residual axillary metastases were found in BLN-positive patients. Considering the 78 patients with SLN positivity or discordant status according to both criteria, the metastases histological size was significantly correlated to the expression level of MG (ρ = 0.62) and CK19 (ρ = 0.64) genes (P < 10E-6). Moreover, ALND status positivity was significantly associated to Ct value of MG (z = 2.4; P = 0.018) and CK19 (z = 3.2; P = 0.001). The high intra-operative quality performance of the BLN assay minimizes the need for second surgeries for ALND. Results from this investigational study suggest that markers Ct value may provide, intra-operatively, valuable metastases size data and a risk prediction of additional disease in non-SLNs.

Use of quantitative RT-PCR assay to predict metastases size of sentinel node from breast cancer patients

621 Background: A RT-PCR Assay (GeneSearch, Veridex, LLC), FDA approved and CE marked to detect metastases &gt; 0.2 mm in sentinel lymph nodes (SLNs) of breast cancer patients, has been in clinical use in our institute for 25 months. The assay gives qualitative (negative/positive) results by applying cutoff values to cycle times (Cts) of mammaglobin (MG) and cytokeratin 19 (CK19) (cutoff values = 30 / 31 Cts). This study evaluates if quantitative Ct values can be used to estimate the probable size of nodal metastases. Methods: To date 384 patients have been clinically tested with the BLN Assay at Institut Jules Bordet. An additional 74 patients were tested in a pilot study. Both sets had SLNs of breast cancer patients divided in ∼2 mm pieces. Alternating pieces were homogenized and intra-operatively processed by the assay. The remaining pieces were used for histological analysis (positive when &gt; 0.2 mm). During clinical use, same-surgery complete axillary dissection was performed when the assay was positive (n=59). Results: Performance of the assay's qualitative results against permanent section H&amp;E was similar for both groups (total N = 458), for a total sensitivity 89% (68/76), specificity 95% (364/382) and overall agreement 94%. The marker Ct values are correlated with metastases size as determined by H&amp;E on adjacent node pieces (r = -0.74 for MG and -0.77 for CK19, p &lt; 0.001). Lower Ct values indicate increased likelihood of macrometastases reported by H&amp;E, as described in the table. Additionally, Assay Ct values in the SLN were predictive of metastases in non-SLN nodes. For example when CK19 and MG &lt; 24 Cts, the non-SLN positivity rate jumps from 29% to 58%. Conclusions: The BLN Assay's high intra- operative qualitative performance minimizes the need for second surgeries for complete axillary dissections. Results from this investigational study examining the marker Ct values suggest that the assay may provide valuable individual tumor volume data intra- operatively or post-operatively. [Table: see text] [Table: see text]

Ipsilateral talar and calcaneal fractures: A retrospective review of complications and sequelae

Background Ipsilateral talar and calcaneal fractures represent a rare combination injury that has only recently been reported in the literature with small case series. Objective To identify the commonly observed fracture patterns, complications, and outcomes of a consecutive series of patients with ipsilateral talar and calcaneal fractures. Methods Forty-five cases of ipsilateral talus and calcanal fractures were identified from an orthopaedic trauma registry at a University-based, level I trauma center for retrospective review. Main outcome measurements Post-operative complications, the need for secondary surgery, and the visual analogus pain score. Results Five patients were treated with an early below knee amputation (BKA). Five patients were treated with a primary subtalar arthrodesis. Twenty-eight of the 35 patients who did not undergo early BKA or primary subtalar arthrodesis developed subtalar arthritis. Five patients had deep wound complications. Four patients had talar body collapse from avascular necrosis. There were 13 open fractures of which 8 resulted in an eventual BKA. The mean visual analogus pain score for the patient population was 4.0. Conclusion The combination of ipsilateral talar and calcaneal fractures represents a severe injury pattern that is associated with significant morbidity. Subtalar arthritis was a common finding regardless of treatment. Open fractures frequently resulted in a below knee amputation.

Quantitative intra-operative RT-PCR assay predicts size of sentinel node metastases.

Abstract Abstract #1002 The GeneSearch™ Breast Lymph Node (BLN) Assay (Veridex, LLC) has been FDA approved to detect metastases &amp;gt; 0.2 mm in the sentinel lymph nodes (SLNs) of breast cancer patients and has been in clinical use in our institute for 11 months. This assay gives a qualitative (negative/positive) result by applying cutoff values to the cycle times (Ct) of the two mRNA markers analyzed: mammaglobin (MG) (cutoff value = 31 Cts) and cytokeratin 19 (CK19) (cutoff value = 30 Cts). This is the first study to evaluate if the quantitative Ct values of MG and CK19 can be used to estimate probable size of nodal metastases.&amp;#x2028; To date 184 patients have been tested using the BLN Assay during its clinical use at Morton Plant Hospital. An additional 172 patients were tested with the BLN Assay at our institute during investigational studies. In both cases, the SLNs of breast cancer patients were divided into ∼2 mm pieces. Alternating pieces were homogenized and were processed intra-operatively. The other pieces of the SLN were used for permanent section H&amp;E analysis. The patient was considered H&amp;E positive when a metastasis &amp;gt; 0.2 mm was identified. During clinical use, same-surgery complete axillary dissection was performed when the assay was positive (n = 34).&amp;#x2028; Performance of the assay's qualitative result against permanent section H&amp;E on adjacent node pieces was similar for the two groups (total N = 356), for a total sensitivity of 96% (81/84), specificity of 93% (254/272), and overall agreement of 94%. The marker Ct values correlated with metastases size as determined by H&amp;E in adjacent node pieces (r = 0.74 for MG and 0.72 for CK19, p &amp;lt; .0001). The lower the Ct values, the greater the likelihood of macrometastases reported by H&amp;E, as described in Table 1. For example if CK19 is &amp;lt;21.0 and MG is &amp;lt; 25.0, there is 100% chance for H&amp;E positivity in the SLNs, with the majority being macrometastases.&amp;#x2028; &amp;#x2028; Additionally, BLN Assay Ct values in the SLN were predictive of metastases in the non-SLN nodes. For example, of the 12 patients who had a SLN with CK19 &amp;lt; 24 Cts, 9 (75%) had H&amp;E positive non-SLNs. For the same 12 patients, SLNs were H&amp;E positive (5 macrometastases, 5 micrometastases, and 2 reported as &amp;gt; 0.2 mm).&amp;#x2028; The BLN Assay's high intra-operative qualitative performance minimizes the need for second surgeries for complete axillary dissections. Results from this investigational study examining the marker Ct values suggest that the assay may provide valuable individual tumor volume data intra-operatively or post-operatively. Citation Information: Cancer Res 2009;69(2 Suppl):Abstract nr 1002.

Ureteral extension in Wilms' tumor: a report from the National Wilms' Tumor Study Group (NWTSG)

Background Extension of Wilms' tumor into the ureter is a rare event. We reviewed the National Wilms' Tumor Study Group (NWTSG) database to define the clinical presentation, associated pathologic features, and clinical outcome of these patients. Methods Records of children identified to have ureteral extension of Wilms' tumor enrolled in NWTS-3, 4, and 5 were reviewed. Presenting symptoms, diagnostic studies, histopathologic findings, operation performed, and outcome were recorded. The NWTS-5 surgical data were prospectively collected as part of the quality assurance program. Results Forty-five children were identified with ureteral extension. For NWTS-5, the incidence of ureteral extension was 2%. Clinical presentations were gross hematuria in 22 patients, 2 had passage of tissue per urethra, and 1 child had a urethral mass. The remainder had nonspecific presentations. Ureteral extension was seen on preoperative imaging in 14 patients, intraoperatively in 22, and on pathologic examination in 9 patients. Hydronephrosis was noted in 12 patients, and there was nonfunction of the kidney in another 8. Laterality of the tumor was right side in 26 and left in 19. Cystoscopy was performed in 12 children. Findings included tumor seen at the ureteral orifice in 6 patients and bleeding from the orifice in one child. All patients had radical nephrectomy including partial ureterectomy. The ureteral margin was positive in 3 patients, including 2 of the 7 with separate removal of the ureteral extension. The number of patients in each clinical stage was as follows: stage I, 10; stage II, 18; stage III, 14; and stage IV, 3. The tumor extended into the proximal ureter in 23 patients, distal ureter in 13, 7 had extension into the bladder, and 1 had urethral involvement. The level of ureteral extension was not clearly noted in the 45th child. The median follow-up was 96 months. Overall, 41 of 45 patients were alive at last contact. There were 3 deaths because of tumor in patients with unfavorable histologic tumors, and 1 because of treatment toxicity in a child with favorable histologic findings. Conclusions Ureteral extension occurs in approximately 2% of patients with Wilms' tumor. The diagnosis should be suspected in patients with gross hematuria, hydronephrosis, or nonfunctioning kidney. Cystoscopy with retrograde ureterogram may aid in preoperative diagnosis in these patients. Preoperative diagnosis is important because complete resection of the involved portion of ureter at the time of nephrectomy can avoid residual disease and the need for second surgery or radiation therapy.

Ligamentum Teres Tenodesis in Medial Approach Open Reduction for Developmental Dislocation of the Hip

When closed reduction of a developmental dislocation of the hip fails, some form of open reduction is required. In recent years, the many advantages of the medial approach open reduction have been emphasized. However, there have been suggestions that the rate of growth disturbance in the proximal femur and the requirement for secondary surgical procedures may be higher with this route than with others. The purpose of this study was to investigate the efficacy and safety of a modified medial approach open reduction, in which the stability of the reduction is enhanced by resection of the redundant ligamentum teres and suturing of the stump of the tendon to the anteromedial capsule. The hospital records and radiographs of 92 infants and children with 109 dislocated hips were reviewed retrospectively. Key demographic and pretreatment data were collected by the first author and compared with the outcome at the most recent follow-up. In addition, 69 children returned for a clinical evaluation by the first author. The outcome at the most recent follow-up was graded according to Severin, and associations were sought between pretreatment grade of dislocation according to Tonnis, the presence of ossific nucleus, changes in the acetabular index, the requirements for secondary surgery, associations with previous treatment, and the position of abduction in the postoperative cast. At a mean follow-up of 9 years, 89% of hips were classified as Severin grade 1 or 2. Avascular necrosis (AVN) was classified according to the system of Kalamchi and MacEwen. The incidence of AVN was 41%, but two thirds of these were grade 1 (temporary irregular ossification), and the Severin grading in these hips was not compromised. The presence of ossification in the capital epiphysis and a range of abduction of less than 60 degrees in the hip spica were noted to be protective against the development of AVN. Three hips redislocated and required additional treatment. Thirty-eight hips required a total of 44 additional surgical procedures. We have demonstrated that it is possible to use a medial approach for open reduction of the congenitally dislocated hip in combination with tenodesis of the ligamentum teres to the anteromedial joint capsule. The incidence of growth disturbance in the proximal femur is high and cumulative with long-term follow-up. However, in this large series, the rate of hip stability, growth disturbance, and need for secondary surgery are comparable to other series. We conclude that the many advantages of open reduction by the medial approach outweigh the disadvantages.

Results of Chondrocyte Implantation with a Fibrin-Hyaluronan Matrix: A Preliminary Study

Fibrin, a homologous polymer, is the natural scaffold of wound healing and therefore a candidate as a carrier for cell transplantation. We explored a novel matrix-based implant cartilage repair composed of both fibrin and hyaluronan in a defined ratio that takes advantage of the biological and mechanical properties of these two elements. The matrix was seeded with autologous chondrocytes expanded in the presence of a proprietary growth factor variant designed to preserve their chondrogenic potential. We prospectively followed eight patients with symptomatic-chronic cartilage defects treated with this carrier. Patients had arthroscopy to harvest autologous chondrocytes then grown in autologous serum. Chondrocytes were cultured in the presence of the FGF variant and then seeded on the fibrin-hyaluronan matrix. About 4 weeks following biopsy, the patients underwent implantation of the constructs by miniarthrotomy. Three of the eight patients had transient effusion. Clinical performance was measured by Lysholm and IKDC scores, MRI, and the need for secondary surgery. The clinical outcome of a 1-year followup demonstrated increase of clinical scores. The MRI followup showed good filling of the defect with tissue having the imaging appearance of cartilage in all patients. Apart from the transient effusion in three patients we observed no other adverse events during the followup. Level IV, therapeutic study.