Near-infrared Research Articles

Intraoperative bladder visualization by indocyanine green filling and subsequent washout in endoscopic hysterectomy: ICG-Washout method.

Despite a potential risk of bladder injury in laparoscopic hysterectomy (LH) and robot-assisted LH (RaLH), an intraoperative method for delineating the entire bladder with indocyanine green (ICG) has not been established. We conducted a preliminary experiment using porcine bladders to verify the appropriate amount of ICG for intraoperative bladder visualization. Afterward, intraoperative bladder visualization was tried in LH and RaLH in two patients suspected of having adhesions around the bladder after previous abdominal surgery. Although near-infrared (NIR) fluorescence was well observed through the wall of the porcine bladder filled with ICG solution at a concentration of 0.024 mg/mL, the subsequent replacement of the ICG solution with saline made the NIR fluorescence brighter. In both patients, the bladder was successfully delineated by NIR fluorescence after filling the bladder with ICG solution and the subsequent washout with saline. The ICG-Washout method for locating the bladder by NIR fluorescence could be useful in LH and RaLH.

Near-infrared AIEgens for sulfatase imaging in breast cancer in vivo.

Aggregation-induced emission luminogens (AIEgens) enable highly sensitive and in situ visualization of sulfatase to benefit the early diagnosis of breast cancer (BC), but current sulfatase AIEgens always emit visible light (<650 nm). Herein, a near-infrared (NIR) AIEgen QMT-SFA is developed for sulfatase imaging in vivo. Hydrophilic QMT-SFA is cleaved by sulfatase to yield hydrophobic QMT-OH, which subsequently aggregates into nanoparticles to turn the AIE fluorescence "on", enabling sensitive sulfatase imaging in 4T1 cells and mouse models.

A Stable Chichibabin Diradicaloid with Near-Infrared Emission.

Conjugated molecules with multiple radical centers such as the iconic Chichibabin diradicaloid hold promise as building blocks in materials for quantum sensing and quantum information processing. However, it is a considerable challenge to design simple analogues of the Chichibabin hydrocarbon that are chemically inert, exhibit high diradical character and emit light at a distinct wavelength that may offer an optical readout of the spin state in functional ensembles. Here we describe the serendipitous discovery of the stable TTM-TTM diradicaloid, which exhibits high diradical character, a striking sky-blue color and near-infrared (NIR) emission (in solution). This combination of properties is unique among related diradicaloids and is due to the presence of hydrogen and chlorine atoms in "just the right positions", allowing a perfectly planar, yet predominantly benzenoid bridge to connect the two sterically stabilized radical centers. In-depth studies of the optical and magnetic properties suggest that this structural motif could become a mainstay building block of organic spin materials.

Ein Stabiles Chichibabin Diradikaloid mit Nahinfraroter Emission

AbstractKonjugierte Moleküle mit mehreren Radikalzentren, wie das ikonische Chichibabin‐Diradikaloid, sind vielversprechende Bausteine für Materialien für die Quantensensorik und Quanteninformationsverarbeitung. Jedoch stellt es eine große Herausforderung dar, einfache Analoga des Chichibabin‐Kohlenwasserstoffs zu entwickeln, die chemisch inert sind, einen hohen Diradikal‐Charakter aufweisen und Licht einer spezifischen Wellenlänge emittieren, was das optische Auslesen des Spinzustands in funktionalen Ensembles ermöglichen würde.

Near-Infrared Absorbing Aza-BODIPY Dyes for Optoelectronic Applications.

Organic dyes that absorb light in the visible to near-infrared region have garnered significant interest, owing to their extensive utility in organic photovoltaics and various biomedical applications. Aza-boron-dipyrromethene (Aza-BODIPY) dyes are a class of chromophores with impressive photophysical properties such as tunable absorption from the visible region towards near infrared (NIR) region, high molar absorptivity, and fluorescence quantum yield. In this review, we discuss the developments in the aza-BODIPYs, related to their synthetic routes, photophysical properties and their applications. Their design strategies, modifications in chemical structures, mode/position of attachment, and their impact on photo-physical properties are reviewed. The potential applications of aza-BODIPY derivatives such as organic solar cells, photodynamic therapy, boron-neutron capture therapy, fluorescence sensors, photo-redox catalysis, photoacoustic probes and optoelectronic devices are explained.

Sunflower Pollen-Morphology Mimicked Spiky Zinc Nanomotors as a Photosensitizer for Killing Bacteria and Cancer Cells.

Photosensitizing agents have received increased attention from the medical community, owing to their higher photothermal efficiency, induction of hyperthermia, and sustained delivery of bioactive molecules to their targets. Micro/nanorobots can be used as ideal photosensitizing agents by utilizing various physical stimuli for the targeted killing of pathogens (e.g., bacteria) and cancer cells. Herein, we report sunflower-pollen-inspired spiky zinc oxide (s-ZnO)-based nanorobots that effectively kill bacteria and cancer cells under near-infrared (NIR) light irradiation. The as-fabricated s-ZnO was modified with a catechol-containing photothermal agent, polydopamine (PDA), to improve its NIR-responsive properties, followed by the addition of antimicrobial (e.g., tetracycline/TCN) and anticancer (e.g., doxorubicin/DOX) drugs. The fabricated s-ZnO/PDA@Drug nanobots exhibited unique locomotory behavior with an average speed ranging from 13 to 14 μm/s under 2.0 W/cm2 NIR light irradiation. Moreover, the s-ZnO/PDA@TCN nanobots exhibited superior antibacterial activity against E. coli and S. epidermidis under NIR irradiation. The s-ZnO/PDA@DOX nanobots also displayed sufficient reactive oxygen species (ROS) amplification in B16F10 melanoma cells and induced apoptosis under NIR light, indicating their therapeutic efficacy. We hope the sunflower pollen-inspired s-ZnO nanorobots have tremendous potential in biomedical engineering from the phototherapy perspective, with the hope to reduce pathogen infections.

Cannabidiol and Aza-BODIPY Coencapsulation for Photodynamic Therapy Enhancement in Liver Cancer Cells.

Photodynamic therapy (PDT) and cannabidiol (CBD) have been explored for their potential in synergistic cancer treatment. In this study, we employed CBD oil as a lipid phase, encapsulated within AZB-I@Lec-T to create lipid-based nanoparticles. Here, CBD oil does two tasks: it acts as a pyroptosis agent to destroy liver cancer cells and as a lipid phase to dissolve the photosensitizer. It was expected that this system would offer synergistic therapy between CBD and PDT better than a single use of each treatment. With a series of in vitro experiments, the nanoparticles exhibited induced apoptosis in 68% of HepG2 cells treated with AZB-I@Lec-T@CBD and near-infrared (NIR)-light irradiation, reducing expression levels of antioxidant defense system genes. Furthermore, both components worked well in a submicromolar range when combined in our formulation. These results highlight the potential for amplifying primary cellular damage with the combination of PDT and CBD encapsulation, providing a promising therapeutic approach for liver cancer treatment guidelines.

Senolytic Therapy Enabled by Senescent Cell-Sensitive Biomimetic Melanin Nano-senolytics.

Cellular senescence is a significant risk factor for aging and age-related diseases (ARD). The canonical senolytics Dasatinib and Quercetin (DQ) have shown promise in clearing senescent cells (SnCs); however, the lack of selectivity poses a challenge in achieving optimal outcomes. Despite the recent occurrence of the nanomaterial-based approaches targeting SnCs, limited therapeutic effects and potential toxicity still remain a major concern. Herein, we developed a "double locks-like" nanoplatform that integrated Galactan coating and mesoporous polydopamine to encase the senolytic drug DQ. By this way, DQ was only released in SnCs that were featured with higher levels of β-galactosidase (β-gal) and low PH. Additionally, the nanoparticles were equipped with 2,2,6,6-Tetramethylpiperidine-1-oxyl (Tempo) to gain enhanced photothermal converting potential. Consequently, the synthesized nanosenolytics demonstrated remarkable specificity and efficacy in eradicating SnCs, and accordingly reversed pulmonary fibrosis in mice without affecting normal tissues. Upon exposure of near-infrared (NIR) light, the nanoparticles demonstrated to efficiently remove senescent tumor cells inducted by chemotherapy, thereby hindering the outgrowth and metastasis or breast cancer. Collectively, the present study develops an "On/Off" switchable nanoplatform in response to SnCs, and produces a more safe, efficient and feasible way to delay aging or alleviate age-associated diseases. This article is protected by copyright. All rights reserved.

Patterned Photonic Actuators with Dynamic Shape‐Morphing and Color‐Changing Capabilities Fabricated by Athermal Embossing Technology

AbstractStimuli‐responsive patterned photonic actuators, characterized by their patterned nano/microscale structures and capacity to demonstrate synergistic color changes and shape morphing in response to external stimuli, have attracted intense scientific attention. However, traditional patterned photonic actuator systems still face limitations such as cumbersome and time‐consuming preparation processes and small‐scale deformations. Herein, we introduce a facile approach involving an athermal embossing technique to rapidly fabricate patterned photonic actuators based on near‐infrared (NIR) light‐responsive liquid crystal elastomers. The resulting patterned photonic actuators demonstrate remarkable features, including brilliant angle‐dependent structural color, complex three‐dimensional actuation, and good color durability under NIR light stimulation. As illustrative demonstrations of the proof‐of‐concept, we fabricate two light‐fuelled patterned photonic soft actuators: a butterfly‐inspired actuator that can produce wing‐flapping dynamic changes in structural color, and an origami crane‐shaped actuator with shape memory, structural color information storage, and dynamic display properties. This strategy provides distinct insights into the design and fabrication of various patterned photonic soft robotic devices and intelligent actuators.

Patterned Photonic Actuators with Dynamic Shape-Morphing and Color-Changing Capabilities Fabricated by Athermal Embossing Technology.

Stimuli-responsive patterned photonic actuators, characterized by their patterned nano/microscale structures and capacity to demonstrate synergistic color changes and shape morphing in response to external stimuli, have attracted intense scientific attention. However, traditional patterned photonic actuator systems still face limitations such as cumbersome and time-consuming preparation processes and small-scale deformations. Herein, we introduce a facile approach involving an athermal embossing technique to rapidly fabricate patterned photonic actuators based on near-infrared (NIR) light-responsive liquid crystal elastomers. The resulting patterned photonic actuators demonstrate remarkable features, including brilliant angle-dependent structural color, complex three-dimensional actuation, and good color durability under NIR light stimulation. As illustrative demonstrations of the proof-of-concept, we fabricate two light-fuelled patterned photonic soft actuators: a butterfly-inspired actuator that can produce wing-flapping dynamic changes in structural color, and an origami crane-shaped actuator with shape memory, structural color information storage, and dynamic display properties. This strategy provides distinct insights into the design and fabrication of various patterned photonic soft robotic devices and intelligent actuators.

Plasmonic Near-Infrared-Response S-Scheme ZnO/CuInS2 Photocatalyst for H2O2 Production Coupled with Glycerin Oxidation.

Solar fuel synthesis is intriguing because solar energy is abundant and this method compensates for its intermittency. However, most photocatalysts can only absorb UV-to-visible light, while near-infrared light remains unexploited. Surprisingly, the charge transfer between ZnO and CuInS2 quantum dots (QDs) can transform a near-infrared-inactive ZnO into a near-infrared-active composite. This strong response is attributed to the increased concentration of free charge carriers in the p-type semiconductor at the interface after the charge migration between ZnO and CuInS2, enhancing the localized surface plasmon resonance effect and the near-infrared (NIR) response of CuInS2. As a paradigm, this ZnO/CuInS2 heterojunction is used for H2O2 production coupled with glycerin oxidation and demonstrates supreme performance, corroborating the importance of near-infrared response and efficient charge transfer. Mechanistic studies through contact potential difference, Hall effect test, and finite element method calculation allow for the direct correlation between the near-infrared response and charge transfer. Our approach bypasses the general light response issues, thereby stepping forward to the ambitious goal of harnessing the entire solar spectrum. This article is protected by copyright. All rights reserved.

A conclusive non-detection of magnetic field in the Am star o Peg with high-precision near-infrared spectroscopy

The A-type metallic-line (Am) stars are typically considered to be non-magnetic or to possess very weak sub-G magnetic fields. This view has been repeatedly challenged in the literature; most commonly for the bright hot Am star Several studies claim to have detected 1--2 kG field of unknown topology in this object, possibly indicating a new process of magnetic-field generation in intermediate-mass stars. In this study, we revisit the evidence of a strong magnetic field in using new high-resolution spectropolarimetric observations and advanced spectral fitting techniques. We estimated the mean magnetic field strength in from the high-precision CRyogenic InfraRed Echelle Spectrograph $) measurement of near-infrared (NIR) sulphur lines. We modelled this observation with a polarised radiative transfer code, including treatment of the departures from local thermodynamic equilibrium. In addition, we used the least-squares deconvolution multi-line technique to derive longitudinal field measurements from archival optical spectropolarimetric observations of this star. Our analysis of the NIR S i lines reveals no evidence of Zeeman broadening, ruling out magnetic field with a strength exceeding 260 G. This null result is compatible with the relative intensification of Fe ii lines in the optical spectrum, taking into account blending and uncertain atomic parameters of the relevant diagnostic transitions. Longitudinal field measurements on three different nights also yield null results with a precision of 2 G. This study refutes the claims of kG-strength dipolar or tangled magnetic field in This star therefore appears to be non-magnetic, with surface magnetic field characteristics no different from those of other Am stars.

The Importance of the Normalized Difference Vegetation Index (NDVI) and the Use of the ArcGIS to create NDVI Maps

This paper studies the Normalized Difference Vegetation Index (NDVI); the creation of the NDVI maps, how these maps are work, and how to create them using ArcMap. These maps sort the vegetation into low and high up to the NDVI values (±0.1). It measures greenness, vegetation health, predicts agricultural productivity, and map desertification; it is an important index in agricultural organizations and environmental studies. It is a standardized index assisting in illustrating the relative biomass, this back to the strong association between its rates and the vegetation density. To calculate the NDVI there is a formula depends on two multi-spectrum’s bands; red light, and near-infrared (NIR). As a result of the contrasts between them, the human see the leaves in green which displays less reflection in the NIR than in red light. Leaves turn into yellow in the NIR and reflect less because of casualty plants, water-stressed, or death.

Activity-based NIR specific fluorescent probe reveals the abnormal elevation of prolyl endopeptidase in hippocampus during Alzheimer's disease progression

Although prolyl endopeptidase (PREP) has become one of the important proteins to investigate the pathogenesis of Alzheimer's Disease (AD), the activity of PREP in the brain of AD patients is still controversial. This study aims to construct a novel enzyme-activated near-infrared (NIR) fluorescence probe for in-situ detection and imaging of PREP in brain, as well as to reveal the relationship between the activity variation of PREP and the development of AD. For these purposes, based on PREP's unique endopeptidase activity catalyzing prolyl-bond hydrolysis and cleavage, four probes deriving from a good NIR fluorophore (ACM) were designed and synthesized. Observations indicated that the Z-GP-ACM exhibited superior selectivity and sensitivity in detecting PREP. In addition, molecular thermodynamic/kinetic calculations and enzymatic kinetics studies also showed that Z-GP-ACM was an excellent substrate for PREP. Subsequent experiments demonstrated that Z-GP-ACM can be used to measure and image endogenous PREP in living cells and mouse brain. We also revealed for the first time that PREP activity was abnormally increased in hippocampus of AD mice during the disease progression. All these results highlighted that Z-GP-ACM could be a promising tool for exploring the biological functions of endogenous PREP in living systems.

Spatiotemporal responsive hydrogel microspheres for the treatment of gastric cancer

AbstractThe development of tumor drug microcarriers has attracted considerable interest due to their distinctive therapeutic performances. Current attempts tend to elaborate on the micro/nano‐structure design of the microcarriers to achieve multiple drug delivery and spatiotemporal responsive features. Here, the desired hydrogel microspheres are presented with spatiotemporal responsiveness for the treatment of gastric cancer. The microspheres are generated based on inverse opals, their skeleton is fabricated by biofriendly hyaluronic acid methacrylate (HAMA) and gelatin methacrylate (GelMA), and is then filled with a phase‐changing hydrogel composed of fish gelatin and agarose. Besides, the incorporated black phosphorus quantum dots (BPQDs) within the filling hydrogel endow the microspheres with outstanding photothermal responsiveness. Two antitumor drugs, sorafenib (SOR) and doxorubicin (DOX), are loaded in the skeleton and filling hydrogel, respectively. It is found that the drugs show different release profiles upon near‐infrared (NIR) irradiation, which exerts distinct performances in a controlled manner. Through both in vitro and in vivo experiments, it is demonstrated that such microspheres can significantly reduce tumor cell viability and enhance the efficiency in treating gastric cancer, indicating a promising stratagem in the field of drug delivery and tumor therapy.

Zinc Doping Engineering in ZnxFe3-xO4 Heterostructures for Enhancing Photodynamic Therapy in the Near-Infrared-II Region.

Currently, photodynamic therapy (PDT) is restricted by the laser penetration depth. Except for PDT at 1064 nm wavelength excitation, the development of other NIR-II-activated nanomaterials with a higher response depth is still hindered and rarely reported in the literature. To overcome these problems, we fabricated a nanoplatform with heterostructures that generate reactive oxygen species (ROS) and ferrite nanoparticles under a high concentration of zinc doping (ZnxFe3-xO4 NPs), which can achieve oxidative damage of tumor cells under near-infrared (NIR) illumination. The recombination of photoelectrons and holes has been markedly inhibited due to the formation of heterostructures in the interfaces, thus greatly enhancing the capability for ROS and oxygen production by modulating the single-component doping content. The efficiency of PDT was verified by in vivo and in vitro assays under NIR light. Our results revealed that NIR-II (1208 nm) light irradiation of ZnxFe3-xO4 NPs exerted a remarkable antitumor activity, superior to NIR-I light (808 nm). More importantly, the reported ZnxFe3-xO4 NPs strategy provides an opportunity for the success of comparison with light in the first and second near-infrared regions.

G-Quadruplex mRNAs Silencing with Inducible Ribonuclease Targeting Chimera for Precision Tumor Therapy.

Ribonuclease targeting chimera (RIBOTAC) represents an emerging strategy for targeted therapy. However, RIBOTAC that is selectively activated by bio-orthogonal or cell-specific triggers has not been explored. We developed a strategy of inducible RIBOTAC (iRIBOTAC) that enables on-demand degradation of G-quadruplex (G4) RNAs for precision cancer therapy. iRIBOTAC is designed by coupling an RNA G4 binder with a caged ribonuclease recruiter, which can be decaged by a bio-orthogonal reaction, tumor-specific enzyme, or metabolite. A bivalent G4 binder is engineered by conjugating a near-infrared (NIR) fluorescence G4 ligand to a noncompetitive G4 ligand, conferring fluorescence activation on binding G4s with synergistically enhanced affinity. iRIBOTAC is demonstrated to greatly knockdown G4 RNAs upon activation under bio-orthogonal or cell-specific stimulus, with dysregulation of gene expressions involving cell killing, channel regulator activity, and metabolism as revealed by RNA sequencing. This strategy also shows a crucial effect on cell fate with remarkable biochemical hallmarks of apoptosis. Mice model studies demonstrate that iRIBOTAC allows selective imaging and growth suppression of tumors with bio-orthogonal and tumor-specific controls, highlighting G4 RNA targeting and inducible silencing as a valuable RIBOTAC paradigm for cancer therapy.

Quantifying Visual Differences in Drought-Stressed Maize through Reflectance and Data-Driven Analysis

Environmental factors, such as drought stress, significantly impact maize growth and productivity worldwide. To improve yield and quality, effective strategies for early detection and mitigation of drought stress in maize are essential. This paper presents a detailed analysis of three imaging trials conducted to detect drought stress in maize plants using an existing, custom-developed, low-cost, high-throughput phenotyping platform. A pipeline is proposed for early detection of water stress in maize plants using a Vision Transformer classifier and analysis of distributions of near-infrared (NIR) reflectance from the plants. A classification accuracy of 85% was achieved in one of our trials, using hold-out trials for testing. Suitable regions on the plant that are more sensitive to drought stress were explored, and it was shown that the region surrounding the youngest expanding leaf (YEL) and the stem can be used as a more consistent alternative to analysis involving just the YEL. Experiments in search of an ideal window size showed that small bounding boxes surrounding the YEL and the stem area of the plant perform better in separating drought-stressed and well-watered plants than larger window sizes enclosing most of the plant. The results presented in this work show good separation between well-watered and drought-stressed categories for two out of the three imaging trials, both in terms of classification accuracy from data-driven features as well as through analysis of histograms of NIR reflectance.

Multispectral-responsive Cu7S4@rGO bio-heterojunction for rapid sterilization through bacterial cuproptosis-like death

Bacterial infection is a significant challenge in the realm of wound healing, particularly in burn and trauma cases, as it delays wound healing, threatens patient health, and promotes the development of drug-resistant bacteria. Although phototherapy is widely employed in the field of antimicrobial therapy, these methods are typically applied in broad spectrum approach to treat various types of infectious diseases. Their selectivity is often limited, which may lead to an increased risk of tissue damage, thereby diminishing clinical applicability and specificity in particular contexts. To address this daunting issue, we have designed a multispectral-responsive bio-heterojunction (bio-HJ) (Cu7S4@rGO bio-HJ) to induce bacteria cuproptosis-like death. Cu7S4@rGO bio-HJ catalytically generates a considerable amount of ROS which assaults the membrane of extracellular bacteria under near infrared (NIR) or visible light. Additionally, Cu7S4@rGO bio-HJ can effectively kill bacteria by inducing localized hyperthermia under NIR. As a result, the multispectral antibacterial properties of Cu7S4@rGO bio-HJ has significantly enhanced its applicability and feasibility in medical settings. Cu7S4@rGO bio-HJ demonstrates potential for utilization in the treatment of oral infections, cutaneous infections (such as acne and herpes), and other superficial infections through its responsiveness to visible light, facilitating photodynamic therapy. Furthermore, in the context of deep-seated tissue infections, Cu7S4@rGO bio-HJ responsiveness to NIR renders it viable for the implementation of combined photodynamic and photothermal therapy. This study reveals the potential of Cu7S4@rGO bio-HJ in multispectral responsiveness and bacterial sterilization, providing new insights for novel biomedical applications.

Enzymes in Synergy: Bacteria Specific Molecular Probe for Locoregional Imaging of Urinary Tract Infection in vivo.

Uropathogenic Escherichia coli(UPECs)is a leading cause for urinary tract infections (UTI), accounting for 70-90% of community or hospital-acquired bacterial infections owing to high recurrence,imprecision in diagnosis and management, and increasing prevalence of antibiotic resistance. Current methods for clinical UPECs detection still rely on labor-intensive urine cultures that impede rapid and accurate diagnosis for timely UTI therapeutic management. Herein, we developed a first-in-class near-infrared (NIR) UPECsfluorescent probe (NO-AH) capable of specifically targetingUPECsthrough itscollaborativeresponse to bacterial enzymes,enabling locoregional imaging of UTIs both in vitroand in vivo.Our NO-AH probe incorporatesa dual protease activatable moiety, which first reactswithOmpT, an endopeptidase abundantly present on outer membrane of UPECs, releasing an intermediate aminoacid residue conjugatedwith a NIR hemicyanine fluorophore. Suchliberated fragment would be subsequentlyrecognized by aminopeptidase (APN) within periplasm of UPECs, activating localized fluorescence forprecise imagingof UTIs in complex living environments.The peculiar specificity and selectivity of NO-AH, facilitated by the collaborative action of bacterial enzymes, features a timely and accurate identification of UPECs-infected UTIs, which could overcomemisdiagnosis inconventional urine tests,thus opening new avenues towards reliable UTI diagnosis and personalized antimicrobial therapy management.