The purpose of this ex-vivo study is to evaluate the efficacy of three irrigation techniques in the reduction of E. faecalis in root canal. Eighty healthy maxillary anteriors were employed in this investigation. The bacteria E. faecalis was left on teeth for 7 days while they were kept. Once teeth were contaminated, they were randomly divided into three groups of 20 for experimentation and a control group of 20 for comparison. NaviTip FX was used to irrigate Group I, the Max-I-probe was used to water Group II, and the Endovac system was used to water Group III. The number of colony forming units (CFUs) was determined by growing bacteria from root canal samples for 2 days in Brain heart infusion agar after chemo-mechanical operations. There was a substantial decrease in bacterial numbers across all experimental groups compared to group IV (control). Group III (Endovac) had the fewest bacterial colonies among the test groups. There were no discernible changes between Group I (the Navitip FX) and Group II (the Max I probe). Endovac was the most effective of the three irrigation methods for reducing CFUs. This study's findings lend credence to the idea that the apical negative pressure approach has more potential than the conventional irrigation delivery system for achieving an enhanced antibacterial impact.