Purpose People with visual impairments or blindness (PwVIB) are mostly excluded from tourism activities. Despite the rise of assistive technology (AT) solutions in Tourism, acceptance remains low because of the difficulty of providing the right functionality, effectiveness and usability. Arguably, it can be said that disability-oriented training can affect the latter two and, therefore, an AT solution’s acceptance. This paper aims to contribute to the theory development and conceptualization of technology acceptance of AT solutions in Tourism by studying, in the context of the Unified Theory of Acceptance and Use of Technology (UTAUT), the effects of training PwVIB on using AT solutions. This study presents the effects of training on the tourism behavior of PwVIB and provides valuable information to the stakeholders. Design/methodology/approach Questionnaire data collected from 128 PwVIB after evaluating an AT were subjected to exploratory and confirmatory factor analysis and structural equation modeling followed by post-evaluation interviews. The used application, called BlindMuseumTourer, enables high-precision autonomous indoor navigation for PwVIB in tourist places like museums and places of health care. Findings The results of this study indicate the partial satisfaction of the extended model validating the importance of performance expectancy and training (the new factor) in predicting the behavioral intention of PwVIB tourists toward using ATs during their tourist activities. This suggests that practitioners have to provide performant technological solutions accompanied by special training sessions for improved engagement and satisfaction. Originality/value This study contributes to the UTAUT theory in the context of Tourism for PwVIB by adding a new factor and replacing two moderator variables. To the best of the authors’ knowledge, no similar work is studying AT acceptance by PwVIB in the tourism literature. Furthermore, the validation process used a novel indoor navigation application, demonstrating its effectiveness in the Tactual Museum of Greece.