With the Covid-19 pandemic, interest in plants and herbal products has increased day by day. The tendency of people towards natural treatment methods has led to more studies on plants and herbal products. The aim of our study was to determine the essential oils of Micromeria congesta, Rosmarinus officinalis L., Sideritis stricta, Artemisia absinthium and Melissa officinalis, which are used in the treatment of various diseases among the people, to evaluate the in vitro antimicrobial activity against Klebsiella pneumoniae, Escherichia coli, Salmonella Typhimurium, Proteus vulgaris, Staphylococcus aureus isolates and Corynebacterium pseudotuberculosis (ATCC 19410) standard strain. The essential oils of M. congesta and M. officinalis plants examined in our study were found to have antibacterial activity as much as reference antibiotics (Streptomycin, Amoxicillin) by disc diffusion method. It was observed that the essential oil of A. absinthium was effective against C. pseudotuberculosis bacteria, while the essential oils of other plants were not effective against the tested bacteria.