This paper presents Serpentine Channel Immersion Cooling (SCIC) as a new design for battery immersion cooling applications using HFE-7100 dielectric fluid. The main objective of this research is to analyze the temperature generation characteristics of Li-ion batteries with the performance of natural convection (NC) and Serpentine Channel Immersion Cooling (SCIC) with HFE-7100 liquid. The depth of discharge (DoD) is carried out at 80% of the battery capacity during the discharge process with variations in C-rates of 1 C, 1.5 C, and 2 C. The results show that the C-rate has a greater value, the temperature value released by the battery will also be greater. The negative pole, intermediate battery, and positive pole exhibit the characteristics of the battery pack’s surface temperature in order from highest to lowest throughout the discharge process. At the negative pole of the battery, the highest temperature in NC cooling is 30.1°C, 32.3°C, and 33.3°C can reduce this temperature to 28.8°C, 29.73°C, 31.8°C by SCIC with HFE-7100 liquid at DoD 1 C, 1.5 C, and 2 C respectively. Battery surface temperature characteristics based on channel position from highest to lowest using NC in the order of 6-3-2-4-5-1 and SCIC in the order of 6-5-4-3-2-1.