Event Abstract Back to Event Diurnal changes in trafficking receptor expression on thymus-deriving natural regulatory T cells Ewelina Kiernozek1*, Anna Kowalik1, Katarzyna Niedzielska1, Magdalena Markowska1 and Nadzieja Drela1 1 University of Warsaw, Poland Natural CD4+CD25+Foxp3+ regulatory T (nTreg) cells play a crucial role in the maintenance of self-tolerance and immune homeostasis. nTregs develop in the thymus and migrate to the periphery as a mature population of T lymphocytes. nTregs can suppress activation, proliferation and effector functions of various immune cells such as helper and cytotoxic T cells, naïve/memory B lymphocytes or dendritic cells. Currently, the mechanisms regulating nTreg development, migration to peripheral lymphoid organs and suppressive function are still explored. As we have shown in our previous study various aspects of nTreg biology are controlled by circadian rhythms. Here we demonstrate that the expression of trafficking receptors show diurnal rhythmicity suggesting the involvement of hormonal regulation in nTreg migration. The aim of the study was to analyse the expression of CD62L and CCR9 on thymus-deriving nTregs of C57BL/6 mice in time intervals correlated with the circadian rhythm of endogenous glucocorticoids synthesis. CD62L direct nTregs to secondary lymphoid tissue and CCR9 to non-lymphoid tissue. We also analysed the expression of these markers after treatment of mice with the synthetic glucocorticoid, dexamethasone. Glucocorticoids play a role in thymocyte development and especially in nTreg fate by inducing the expression of transcriptional factor Foxp3. C57BL/6 mice were injected i.p. with Dex at the dose of 1,25mg/kg/day. The analysis of nTreg markers expression was synchronized with the circadian rhythm of glucocorticoids synthesis. The results of the first part our studies in brief indicate that after administration of dexamethasone 1/ the differences in trafficking receptor expression were gender-dependent 2/ trafficking receptors expression on nTregs reveal diurnal rhythmicity, which was in accordance to circadian rhythm of glucocorticoids synthesis. Acknowledgements This study was supported by Grant No. N N303 395836 from the MNiSW (Poland) Keywords: nTreg, trafficking receptor expression, CD62L, CCR9, diurnal rhythmicity Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Adaptive Immunity Citation: Kiernozek E, Kowalik A, Niedzielska K, Markowska M and Drela N (2013). Diurnal changes in trafficking receptor expression on thymus-deriving natural regulatory T cells . Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00746 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 13 Jun 2013; Published Online: 22 Aug 2013. * Correspondence: Miss. Ewelina Kiernozek, University of Warsaw, Warsaw, Poland, ekiernozek@biol.uw.edu.pl Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Ewelina Kiernozek Anna Kowalik Katarzyna Niedzielska Magdalena Markowska Nadzieja Drela Google Ewelina Kiernozek Anna Kowalik Katarzyna Niedzielska Magdalena Markowska Nadzieja Drela Google Scholar Ewelina Kiernozek Anna Kowalik Katarzyna Niedzielska Magdalena Markowska Nadzieja Drela PubMed Ewelina Kiernozek Anna Kowalik Katarzyna Niedzielska Magdalena Markowska Nadzieja Drela Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.