Native Core Research Articles

Graphene Nanoplatelets Extend Life of Scale-Inhibitor Squeeze Treatment

_ This article, written by JPT Technology Editor Chris Carpenter, contains highlights of paper SPE 215228, “Graphene Nanoplatelets for Extended Lifetime of Scale-Inhibitor Squeeze Treatment in a High-Temperature Reservoir,” by Suzalina Zainal, SPE, Norzafirah Razali, and Mohamad A. Rodzali, Petronas, et al. The paper has not been peer reviewed. _ Scale-inhibitor squeeze (SISQ) treatment is an established method for offshore fields that allows an extended effectiveness of scale inhibitors in preventing deposition over time. One of the primary issues in SISQ campaigns is the short squeeze lifetime of less than 1 year. The study detailed in the complete paper proposed graphene nanoplatelets (GNP) as a preflush system to extend the lifetime of a conventional SISQ treatment. This carbon-based nanomaterial allowed enhanced adsorption of the phosphonate-based scale inhibitor. GNP as a Preflush System A high-surface-area GNP preflush system was chosen to condition the rock surface to overcome the apparent lack of suitable surfaces available for scale-inhibitor adsorption during SISQ treatment. The GNP was injected to coat the rock surface. GNPs consist of a few graphite layers with thicknesses varying from 0.7 to 100 nm. The carbon-based nanomaterial forms a honeycomb structure, regarded as a good alternative to its predecessor, graphene, because of its lower cost and potential for large-scale production. GNP particle size ranges between 100 and 200 nm. This 2D carbon nanomaterial offers several advantages, such as high surface area, a high aspect ratio with a planar shape, and good mechanical properties with excellent thermal and electrical conductivities. It is also inert toward common reservoir fluid. Experimental Procedure Materials. Injection and formation water (brine samples) were prepared based on compositions listed in Table 1 of the complete paper. Berea core plugs were used for static-adsorption and injectivity experiments. In these two experiments, the core samples were used to provide similar rock properties when comparing performances of the baseline preflush (seawater) vs. nano-preflush systems. The average rock permeability for the candidate oil field is between 200 and 400 md. Dispersion Evaluation in High Divalent Brine. Gum arabic (GA) stabilized the GNP in high divalent brine. The polymer-grafted GNP (P-GNP) samples were evaluated in terms of exfoliated sites as compared with GNP alone. Zeta-potential and particle-size analysis also were performed at different P-GNP concentrations. Static-Adsorption Evaluation. A static-adsorption method was designed to replicate preflush injection of P-GNP before introduction of scale inhibitor. Experiments compared the adsorption of the scale inhibitor onto crushed Berea rock, precoated with P-GNP, with another without pre-coating that used seawater preflush only. Coreflood Evaluation. Because of the scarcity of the native core samples, only Berea core samples were used for the injectivity study, while native core samples were used for the SISQ adsorption/desorption coreflood evaluation. In both types of experiments, a P-GNP preflush system was compared with the baseline (seawater preflush).

Superiority of native soil core microbiomes in supporting plant growth

Native core microbiomes represent a unique opportunity to support food provision and plant-based industries. Yet, these microbiomes are often neglected when developing synthetic communities (SynComs) to support plant health and growth. Here, we study the contribution of native core, native non-core and non-native microorganisms to support plant production. We construct four alternative SynComs based on the excellent growth promoting ability of individual stain and paired non-antagonistic action. One of microbiome based SynCom (SC2) shows a high niche breadth and low average variation degree in-vitro interaction. The promoting-growth effect of SC2 can be transferred to non-sterile environment, attributing to the colonization of native core microorganisms and the improvement of rhizosphere promoting-growth function including nitrogen fixation, IAA production, and dissolved phosphorus. Further, microbial fertilizer based on SC2 and composite carrier (rapeseed cake fertilizer + rice husk carbon) increase the net biomass of plant by 129%. Our results highlight the fundamental importance of native core microorganisms to boost plant production.

Bending performance and failure mechanisms of composite sandwich structures with 3D printed hybrid triply periodic minimal surface cores

In this paper, a novel hybrid triply periodic minimal surface (TPMS) core with a nonlinear transition region was designed by combining two types of TPMS (Diamond and Schwarz P) structures using the sigmoid function. The width of the transition region was precisely regulated by adjusting the gradient control parameter r in the sigmoid function. Composite sandwich structures (CSS) were fabricated by bonding two carbon fiber reinforced polymer (CFRP) face sheets to a 3D printed polylactic acid (PLA) core. The bending performance and failure mechanisms of the CSSs with the hybrid TPMS cores were analyzed through three-point bending tests and finite element analysis (FEA). The results indicate that as r increases, the transition region of the hybrid TPMS cores becomes narrower, leading to a gradual decrease in bending strength, bending stiffness, and core shear stress. The failure process of the CSSs in the experiment aligns well with the FEA results. Through comparative analysis of the stiffness-to-weight and strength-to-weight ratios of the CSSs with the native TPMS cores, the hybrid TPMS cores with a wider transition region enhance the structural efficiency of the CSSs, while those with a narrower transition region negatively impact the performance of the CSSs.

Engineering a carbon source-responsive promoter for improved biosynthesis in the non-conventional yeast Kluyveromyces marxianus

Many desired biobased chemicals exhibit a range of toxicity to microbial cell factories, making industry-level biomanufacturing more challenging. Separating microbial growth and production phases is known to be beneficial for improving production of toxic products. Here, we developed a novel synthetic carbon-responsive promoter for use in the rapidly growing, stress-tolerant yeast Kluyveromyces marxianus, by fusing carbon-source responsive elements of the native ICL1 promoter to the strong S. cerevisiae TDH3 or native NC1 promoter cores. Two hybrids, PIT350 and PIN450, were validated via EGFP fluorescence and demonstrated exceptional strength, partial repression during growth, and late phase activation in glucose- and lactose-based medium, respectively. Expressing the Gerbera hybrida 2-pyrone synthase (2-PS) for synthesis of the polyketide triacetic acid lactone (TAL) under the control of PIN450 increased TAL more than 50% relative to the native NC1 promoter, and additional promoter engineering further increased TAL titer to 1.39 g/L in tube culture. Expression of the Penicillium griseofulvum 6-methylsalicylic acid synthase (6-MSAS) under the control of PIN450 resulted in a 6.6-fold increase in 6-MSA titer to 1.09 g/L and a simultaneous 1.5-fold increase in cell growth. Finally, we used PIN450 to express the Pseudomonas savastanoi IaaM and IaaH proteins and the Salvia pomifera sabinene synthase protein to improve production of the auxin hormone indole-3-acetic acid and the monoterpene sabinene, respectively, both extremely toxic to yeast. The development of carbon-responsive promoters adds to the synthetic biology toolbox and available metabolic engineering strategies for K. marxianus, allowing greater control over heterologous protein expression and improved production of toxic metabolites.

Five-Year Outcomes After Bicuspid Aortic Valve Replacement With a Novel Tissue Bioprosthesis

BackgroundThis study investigated the safety and effectiveness of surgical aortic valve replacement with RESILIA tissue (Edwards Lifesciences) through 5 years in patients with native bicuspid aortic valves. Outcomes were compared with those for patients with tricuspid aortic valves. MethodsOf 689 patients from the COMMENCE (ProspeCtive, nOn-randoMized, MulticENter) trial who received the study valve, 645 had documented native valve morphology and core laboratory-evaluable echocardiograms from any postoperative visit, which were used to model hemodynamic outcomes over 5 years. Linear mixed-effects models were used to estimate longitudinal changes in mean gradient and effective orifice area. ResultsPatients with native bicuspid aortic valves (n = 214) were more than a decade younger than those with tricuspid aortic valves (n = 458; 59.8 ± 12.4 years vs 70.2 ± 9.5 years; P < .001). The bicuspid aortic valve cohort exhibited no structural valve deterioration over 5 years, and rates of paravalvular leak and transvalvular regurgitation were low (0.7% and 2.9%, respectively [all mild] at 5 years). These outcomes mirrored those in patients with native tricuspid aortic valves. The model-estimated postoperative mean gradient and effective orifice area, as well as the rate of change of these outcomes, adjusted for age, body surface area, and bioprosthesis size, did not differ between the 2 cohorts. ConclusionsAmong patients with bicuspid aortic valves, RESILIA tissue valves demonstrated excellent outcomes to 5 years, including no structural valve deterioration and very low rates of paravalvular and transvalvular regurgitation. These results are encouraging for RESILIA tissue durability in young patients.

Structure of the native chemotaxis core signaling unit from phage E-protein lysed E. coli cells.

Bacterial chemotaxis is a ubiquitous behavior that enables cell movement toward or away from specific chemicals. It serves as an important model for understanding cell sensory signal transduction and motility. Characterization of the molecular mechanisms underlying chemotaxis is of fundamental interest and requires a high-resolution structural picture of the sensing machinery, the chemosensory array. In this study, we combine cryo-electron tomography and molecular simulation to present the complete structure of the core signaling unit, the basic building block of chemosensory arrays, from Escherichia coli. Our results provide new insight into previously poorly-resolved regions of the complex and offer a structural basis for designing new experiments to test mechanistic hypotheses.

Fibroin heavy chain gene replacement with a highly ordered synthetic repeat sequence in Bombyx mori

The exceptional quality of silkworm silk is attributed to the amino acid sequence of its fibroin heavy chain (Fib-H) protein. The large central domain of Fib-H, which consists of glycine- and alanine-rich crystalline regions interspersed with amorphous motifs of approximately 30 amino acid residues, is considered crucial for fibrilization and determines the properties of the silk fiber. We established a technical platform to modify the Fib-H core region systematically using transcription activator-like effector nuclease-mediated homologous recombination through a somatic and germline gene knockin assay along with PCR-based screening. This efficient knockin system was used to generate a silkworm strain carrying a mutant Fib-H allele, in which the core region was replaced with a highly ordered synthetic repeat sequence of a length comparable with native Fib-H core. Heterozygous knockin mutants produced seemingly normal cocoons, whereas homozygotes did not and exhibited considerable degradation in their posterior silk glands (PSGs). Cross-sectional examination of the PSG lumen and tensile tests conducted on reeled silk threads indicated that the mutant Fib-H, which exhibited reduced stability in the PSG cells and lumen, affected the mechanical properties of the fiber. Thus, sequence manipulation of the Fib-H core domain was identified as a crucial step in successfully creating artificial silk using knockin technology.

Phylogenetic distance affects the artificial microbial consortia’s effectiveness and colonization during the bioremediation of polluted soil with Cr(VI) and atrazine

Soils co-contaminated with heavy metals and organic pollutants are common and threaten the natural environment and human health. Although artificial microbial consortia have advantages over single strains, the mechanism affecting their effectiveness and colonization in polluted soils still requires determination. Here, we constructed two kinds of artificial microbial consortia from the same or different phylogenetic groups and inoculated them into soil co-contaminated with Cr(VI) and atrazine to study the effects of phylogenetic distance on consortia effectiveness and colonization. The residual concentrations of pollutants demonstrated that the artificial microbial consortium from different phylogenetic groups achieved the highest removal rates of Cr(VI) and atrazine. The removal rate of 400 mg/kg atrazine was 100%, while that of 40 mg/kg Cr(VI) was 57.7%. High-throughput sequence analysis showed that the soil bacterial negative correlations, core genera, and potential metabolic interactions differed among treatments. Furthermore, artificial microbial consortia from different phylogenetic groups had better colonization and a more significant effect on the abundance of native core bacteria than consortia from the same phylogenetic group. Our study highlights the importance of phylogenetic distance on consortium effectiveness and colonization and offers insight into the bioremediation of combined pollutants.

Fragmentation disrupts microbial effects on native plant community productivity

Abstract Anthropogenic habitat fragmentation—the breaking up of natural landscapes—is a pervasive threat to biodiversity and ecosystem function world‐wide. Fragmentation results in a mosaic of remnant native habitat patches embedded in human‐modified habitat known as the ‘matrix’. By introducing novel environmental conditions in matrix habitats and reducing connectivity of native habitats, fragmentation can dramatically change how organisms experience their environment. The effects of fragmentation can be especially important in urban landscapes, which are expanding across the globe. Despite this surging threat and the importance of microbiomes for ecosystem services, we know very little about how fragmentation affects microbiomes and even less about their consequences for plant–microbe interactions in urban landscapes. By combining field surveys, microbiome sequencing and experimental mesocosms, we (1) investigated how microbial community diversity, composition and functional profiles differed between 15 native pine rockland fragments and the adjacent urban matrix habitat, (2) identified habitat attributes that explained significant variation in microbial diversity of native core habitat compared to urban matrix and (3) tested how changes in urbanized and low connectivity microbiomes affected plant community productivity. We found urban and native microbiomes differed substantively in diversity, composition and functional profiles, including symbiotic fungi decreasing 81% and pathogens increasing 327% in the urban matrix compared to native habitat. Furthermore, fungal diversity rapidly declined as native habitats became increasingly isolated, with ~50% of variation across the landscape explained by habitat connectivity alone. Interestingly, microbiomes from native habitats increased plant productivity by ~300% while urban matrix microbiomes had no effect, suggesting that urbanization may decouple beneficial plant–microbe interactions. In addition, microbial diversity within native habitats explained significant variation in plant community productivity, with higher productivity linked to more diverse microbiomes from more connected, larger fragments. Synthesis. Taken together, our study not only documents significant changes in microbial diversity, composition and functions in the urban matrix, but also supports that two aspects of habitat fragmentation—the introduction of a novel urban matrix and reduced habitat connectivity—disrupt microbial effects on plant community productivity, highlighting preservation of native microbiomes as critical for productivity in remnant fragments.

Geochemical characteristics of spherules from the Pivdenna kimberlite pipe, East Azov region (Ukraine): implications for their sources and origin

The composition of spherules and particles of native metals from the Pivdenna kimberlite pipe, Ukraine, was studied using the SEM/EDS method. Three varieties of spherules have been distinguished: titanium-manganese-iron-silicate (TMIS) spherules, Ca-rich silicate spherules, and magnetite-wustite-iron (MW-I) spherules. TMIS spherules are composed of homogeneous glass, some having a native iron core. Large TMIS spherules may contain a crystalline phase with needle-like armalcolite. Ca-rich silicate spherules can be subdivided into two subtypes: calcium-silicate (CS) spherules where SiO2 and CaO are the dominant constituents, and calcium-iron-silicate (CIS) spherules with significant FeO content. CS spherules may contain a core consisting of native phases (Fe, Fe-Si, and Mn-Si-Fe). Native metal particles are represented by native Cu and native Zn. The spherule varieties from the Pivdenna pipe are similar to those from other kimberlite pipes in the world. We infer that the formation of spherules occurred in gas-melt streams, separately from the kimberlites, and propose a model for the formation of the most common variety of spherules (TMIS and MW-I varieties) in the region of the core-mantle boundary (CMB). First, a melt of the Fe-Ti-Mn-Si-O system was formed in ultra-low-velocity zones (ULVZ) as a result of thermochemical reactions (reduction) between the molten core and solid oxide-silicate rocks. The melt then migrates to shallower levels, where a decrease in temperature initiates oxidation with the formation of SiO2-TiO2-FeO-MnO-Fe0 melt, i.e. parent melt of TMIS and MW-I spherules. We interpret the formation of native metals in kimberlites as a result of the decomposition of nitrides, which came from the Earth’s core via intratelluric flows

Magnetic Coupling in Cobalt-Doped Iron Oxide Core–Shell Nanoparticles: Exchange Pinning through Epitaxial Alignment

Tuning the core–shell morphology of bimagnetic nanoparticles and its associated exchange bias behavior is a promising way to overcome the superparamagnetic limit and stabilize the particle moment in extended time and temperature ranges. The intraparticle magnetization distribution and magnetic coupling between the two phases, however, is still unclear. We report a significant nonzero magnetization in the CoxFe(1–x)O core of native core–shell bimagnetic nanoparticles that is typically considered antiferro- or paramagnetic. Co0.14Fe0.86O@Co0.4Fe2.4O4 (6 nm@2 nm) and Co0.08Fe0.92O@Co0.58Fe2.28O4 (12 nm@2 nm) core–shell nanoparticles have been synthesized by thermal decomposition of a mixed cobalt–iron oleate with a similar Fe/Co distribution throughout the nanoparticle. We determine the exact phase composition and the magnetization distribution in the core and shell using a combination of X-ray and neutron small-angle scattering. Core and shell magnetization are traced separately with a varying magnetic field. Our results reveal that the magnetization of the core and the spinel-type shell phases are coupled at room temperature, i.e., rotating coherently with the magnetic field. This is a mandatory condition to observe a significant exchange bias effect at low temperatures. These findings highlight the enormous potential of finite size and exchange coupling in bimagnetic nanoparticles to control the magnetic properties via interface-induced magnetization.

Evolution of electrical resistivity and NMR during early-stage maturation of an organic-rich chalk

This paper studies the changes in the electrical resistivity of an organic-rich source rock chalk containing a Type-IIs kerogen during early-stage maturation of the kerogen. As bitumen is generated during maturation, the chalk changes from water wet to a complex mixed-wet pore system, with the micritic pore space remaining water wet while the bitumen expelled into the macro pores converts some pores to oil wet. This mixed-wet system results in non-Archie electrical behavior with pronounced curvature of the I-Sw curve. We find that this curvature is well fit by connectivity theory. The connectivity theory parameters are determined both on native state core and from artificial laboratory pyrolysis which generates bitumen. NMR is used to distinguish the brine in micritic, water-wet pore spaces from that in the intergranular, mixed-wet pore spaces, from which the observed connectivity parameters may be independently estimated. Comparison between electrical resistivity and dielectric logs show that the connectivity parameters match the laboratory results over a calcareous section more than 300 m thick.

Model Configuration versus Driving Model: Influences on Next-Day Regional Convection-Allowing Model Forecasts during a Real-Time Experiment

Abstract As part of NOAA’s Hazardous Weather Testbed Spring Forecasting Experiment (SFE) in 2020, an international collaboration yielded a set of real-time convection-allowing model (CAM) forecasts over the contiguous United States in which the model configurations and initial/boundary conditions were varied in a controlled manner. Three model configurations were employed, among which the Finite Volume Cubed-Sphere (FV3), Unified Model (UM), and Advanced Research version of the Weather Research and Forecasting (WRF-ARW) Model dynamical cores were represented. Two runs were produced for each configuration: one driven by NOAA’s Global Forecast System for initial and boundary conditions, and the other driven by the Met Office’s operational global UM. For 32 cases during SFE2020, these runs were initialized at 0000 UTC and integrated for 36 h. Objective verification of model fields relevant to convective forecasting illuminates differences in the influence of configuration versus driving model pertinent to the ongoing problem of optimizing spread and skill in CAM ensembles. The UM and WRF configurations tend to outperform FV3 for forecasts of precipitation, thermodynamics, and simulated radar reflectivity; using a driving model with the native CAM core also tends to produce better skill in aggregate. Reflectivity and thermodynamic forecasts were found to cluster more by configuration than by driving model at lead times greater than 18 h. The two UM configuration experiments had notably similar solutions that, despite competitive aggregate skill, had large errors in the diurnal convective cycle.

Structures and comparison of endogenous 2-oxoglutarate and pyruvate dehydrogenase complexes from bovine kidney

The α-keto acid dehydrogenase complex family catalyzes the essential oxidative decarboxylation of α-keto acids to yield acyl-CoA and NADH. Despite performing the same overarching reaction, members of the family have different component structures and structural organization between each other and across phylogenetic species. While native structures of α-keto acid dehydrogenase complexes from bacteria and fungi became available recently, the atomic structure and organization of their mammalian counterparts in native states remain unknown. Here, we report the cryo-electron microscopy structures of the endogenous cubic 2-oxoglutarate dehydrogenase complex (OGDC) and icosahedral pyruvate dehydrogenase complex (PDC) cores from bovine kidney determined at resolutions of 3.5 Å and 3.8 Å, respectively. The structures of multiple proteins were reconstructed from a single lysate sample, allowing direct structural comparison without the concerns of differences arising from sample preparation and structure determination. Although native and recombinant E2 core scaffold structures are similar, the native structures are decorated with their peripheral E1 and E3 subunits. Asymmetric sub-particle reconstructions support heterogeneity in the arrangements of these peripheral subunits. In addition, despite sharing a similar monomeric fold, OGDC and PDC E2 cores have distinct interdomain and intertrimer interactions, which suggests a means of modulating self-assembly to mitigate heterologous binding between mismatched E2 species. The lipoyl moiety lies near a mobile gatekeeper within the interdomain active site of OGDC E2 and PDC E2. Analysis of the twofold related intertrimer interface identified secondary structural differences and chemical interactions between icosahedral and cubic geometries of the core. Taken together, our study provides a direct structural comparison of OGDC and PDC from the same source and offers new insights into determinants of interdomain interactions and of architecture diversity among α-keto acid dehydrogenase complexes.

Dispersed ice of permafrost peatlands represents an important source of labile carboxylic acids, nutrients and metals

Thawing of frozen organic and mineral soils and liberation of organic carbon (OC), macro- and micro-nutrients and trace elements from pore ice in high latitude regions represent a potentially important but poorly quantified retroactive linkage to climate warming. This is especially true for permafrost peatlands, occupying a sizable proportion of all permafrost territories and presenting a large and highly vulnerable stock of soil OC which can be subjected to fast thawing at currently circum-zero temperatures. The conventional method of assessing the labile water-soluble fraction of permafrost soils is aqueous extraction from dried soil. However, this technique does not allow collecting native ice present in soil pores and is therefore likely to underestimate or overestimate the pool of labile soil C and nutrients. Here, we present results of direct pore ice analyses performed on native peat cores from the western Siberia Lowland in comparison to the water extraction (10 and 100 gdry peat L-1) of soluble components from the same peat subjected to freeze drying. Aqueous leachates of permafrost peat from both thawed (0–45 cm) and frozen (45–130 cm) layers yielded high concentrations of DOC, nutrients, carboxylic acids and trace metals, comparable or higher to those in peat porewater and dispersed peat ice. We found strong (a factor of 3 to 30) enrichment in the frozen part of the core (below 45 cm, which is active layer depth) in dissolved OC, many carboxylates (acetate, formate, lactate, butyrate, propionate, pyruvate), inorganic nutrients (Si, P, N) and trace elements (Fe, Al, Mn, Zn, Sr and Ba). The dispersed ice which is present in peat below active layer represents highly labile reservoir of organic and inorganic nutrients which should be considered in permafrost thaw scenarios.

Signal Peptide-rheostat Dynamics Delay Secretory Preprotein Folding

Sec secretory proteins are distinguished from cytoplasmic ones by N-terminal signal peptides with multiple roles during post-translational translocation. They contribute to preprotein targeting to the translocase by slowing down folding, binding receptors and triggering secretion. While signal peptides get cleaved after translocation, mature domains traffic further and/or fold into functional states. How signal peptides delay folding temporarily, to keep mature domains translocation-competent, remains unclear. We previously reported that the foldon landscape of the periplasmic prolyl-peptidyl isomerase is altered by its signal peptide and mature domain features. Here, we reveal that the dynamics of signal peptides and mature domains crosstalk. This involves the signal peptide’s hydrophobic helical core, the short unstructured connector to the mature domain and the flexible rheostat at the mature domain N-terminus. Through this cis mechanism the signal peptide delays the formation of early initial foldons thus altering their hierarchy and delaying mature domain folding. We propose that sequence elements outside a protein’s native core exploit their structural dynamics to influence the folding landscape.

FIELD TEST OF THE INDIGENOUS MICROBES FOR OIL RECOVERY, LEDOK FIELD, CENTRAL JAVA

After selecting several old wells in Cepu, then well LDK-132 was chosen for implementing MEOR technology using "huff and puff" method. For this purpose, fluids samples (water and oil) from Ledok Formation were taken at the wellhead. No core of LDK-132 was available, only cores from LDK-209 and LDK-P1 were found. Core plugs could not be made from the former due to compacted and tightly conditions with a very low permeability of only 0.458 md. The latter with the permeability of 140 md met the requirements of microbial core flooding (MCF) tests, A standard quartz dominated core from Clashach Scotland with permeability almost 800 md was also used to compare with the native core's results. The MCF tests were conducted at the reservoir conditions for both types of core. The native core gave a recovery factor of oil production of 12.58%, while the standard core yielded a higher recovery fac- tor of 21.22% of Sor. Based on these results, the MEOR implementation was conducted on July 8, 1999 by injecting 135 barrels of mixtures consist- ing of formation water, microbes enriched with KKL-11 (Koleksi Kultur Lemigas or Lemigas Cultures Collection) and M4 medium through the annulus of LDK-132. The result showed an increase of average oil production rate, from 3.46 bopd, 6 days before the injection, to 24.85 bopd, 6 days after the injection.

Tracer Tests For Heterogeneity Characterization And Saturation Determination On Core Flooding

Low sweep efficiency is the common problem in displacement process due to heterogeneity, high permeability streaks, fractures, and thief zones existing in the formation. Similarly, the success or failure of EOR implementations are always affected by those problems which causes displacing fluids fingering and early breakthrough. Factors of this type, unless properly identified and understood before the start of EOR process, will likely cause a project failure. Core flooding as the model of small scale of fluids movements in reservoir undergoes similar circumstances. Approximately one foot long of four 3.5 inches stacked native and synthetic cores are normally used in core flooding experiment. Tracer test was performed to characterize the core in addition of CT scan analysis. On this experiment, lithium solution was selected as tracer solution to be then injected into core at constant rate, 4 ft/day. Afterwards, the effluents were collected by Gilson sample collector in each tube for further determinination its concentration using Atomic Absorption Spectrometry (AAS). Response curves of lithium tracer were able to determine core heterogeneities and this should be done to avoid misleading interpretation of core flooding results. Besides, lithium concentration reported in some extent and subsequently analyzed by employing method of temporal moments. This method provides numerical calculation to estimate effective core pore volume (PV) and fluid saturation. Weighing method was also used to compare the PV with aforementioned method and the results were comparable.

Influence of the Dynamically Disordered N-Terminal Tail Domain on the Amyloid Core Structure of Human Y145Stop Prion Protein Fibrils.

The Y145Stop mutant of human prion protein (huPrP23-144) is associated with a familial prionopathy and provides a convenient in vitro model for investigating amyloid strains and cross-seeding barriers. huPrP23-144 fibrils feature a compact and relatively rigid parallel in-register β-sheet amyloid core spanning ∼30 C-terminal amino acid residues (∼112–141) and a large ∼90-residue dynamically disordered N-terminal tail domain. Here, we systematically evaluate the influence of this dynamic domain on the structure adopted by the huPrP23-144 amyloid core region, by investigating using magic-angle spinning solid-state nuclear magnetic resonance (NMR) spectroscopy a series of fibril samples formed by huPrP23-144 variants corresponding to deletions of large segments of the N-terminal tail. We find that deletion of the bulk of the N-terminal tail, up to residue 98, yields amyloid fibrils with native-like huPrP23-144 core structure. Interestingly, deletion of additional flexible residues in the stretch 99–106 located outside of the amyloid core yields shorter heterogenous fibrils with fingerprint NMR spectra that are clearly distinct from those for full-length huPrP23-144, suggestive of the onset of perturbations to the native structure and degree of molecular ordering for the core residues. For the deletion variant missing residues 99–106 we show that native huPrP23-144 core structure can be “restored” by seeding the fibril growth with preformed full-length huPrP23-144 fibrils.

Critical rate analysis for CO2 injection in depleted gas field, Sarawak Basin, offshore East Malaysia

This study aimed to address the challenges and strategies to determine the critical rate of CO2 injection into a carbonate depleted gas field. In this research, the critical rate is the maximum allowable injection rate before formation damage initiation. The cause of formation damage could be due to in-situ mobilization or trapping of migratory fines resulting in plugging the flow path. This study performed a thorough investigation of a different rock-fluid system to evaluate the safe injection limit, as the critical rate is different for each rock-fluid system. The geochemical effect of CO2 injection toward carbonate formation was also investigated in this research. Other than that, the porosity and permeability changes due to CO2-brine-rock multiphase flow characteristics were considered to understand the feasibility of CO2 sequestration into carbonate formation. This research discussed experimental design to mimic the CO2 injection scenario of CO2 into carbonate depleted gas field. Therefore, several core flooding experiments were conducted under reservoir conditions using representative native cores, CO2, and synthetic formation brine. Abrupt changes in differential pressure (ΔP), analysis of effluent collected after CO2 multi-rate flow, and pH reading are the key indicators to consider that the condition has reached a critical rate. The experimental result demonstrated the existence of fines migration, scale formation, and salt precipitation after the core was subjected to supercritical CO2 multi-rate flow. Considering these issues and challenges associated with injectivity, this study recommended a maximum injection rate prior to field scale injection.