Abstract Background Health care workers (HCWs) are a trusted source of vaccine information for patients and communities. Our aim is to evaluate the impact of the COVID-19 pandemic on European HCWs’ sentiment towards vaccination (STV), focusing on COVID-19 and influenza. Methods We designed a multilingual Knowledge-Attitudes-Practice web-survey with 4-point Likert items addressed to HCWs in 11 countries and spread through national Public Health Associations. We present a preliminary analysis of responses from France (n = 206), Germany (n = 376), Italy (n = 394), Spain (n = 549) and the United Kingdom (n = 172, UK). Results The completion rate is 81% (1,374/1,697). Respondents are mostly female (66%), with a median age of 45, mainly employed as physicians (44%). HCWs agree on the effectiveness (92%), safety (89%) and necessity (91%) of vaccinations in general, but many think that their side effects are downplayed by pharmaceutical companies (46%) and health authorities (39%). The majority received 3+ doses of COVID-19 vaccines (72%) and agree that these vaccines are effective (88%) and have a favorable benefit-risk ratio (83%). However, many have concerns about their administration in pregnancy (76%). Most HCWs agree with mandatory COVID-19 vaccine for their category (59%) and are ready to accept a further COVID-19 booster vaccination if recommended (72%). The majority agree that the pandemic increased their attention to update their vaccination schedule (62%) and their confidence in vaccines safety (53%). Accordingly, only 50% received regular influenza vaccination prior to the pandemic, whereas 67% got vaccinated in the last season and 65% plan to do so in the next one. Overall, STV is more positive in Italy and Spain, less marked in Italy has the lowest scores in Germany. Conclusions Despite persisting doubts about COVID-19 vaccines and differences among countries, the sentiment towards vaccinations in European HCWs is generally positive and has improved after the pandemic. Key messages • European healthcare workers have generally positive sentiment towards vaccination, including COVID-19 and influenza, despite concerns about side effects and about administration during pregnancy. • The COVID-19 pandemic increased European HCWs’ attention to update their vaccination schedules, with the majority agreeing on the effectiveness, safety, and necessity of vaccinations in general.