National Livestock Research Institute Research Articles

Relationship between strains of Holstein cows, feeding strategies and udder health

Factores relacionados con el animal y el ambiente pueden afectar la frecuencia de mastitis, siendo la genética y la estrategia de alimentación algunos de dichos factores. El objetivo de este estudio fue analizar la frecuencia de mastitis clínica y los recuentos de células somáticas (RCS) de vacas Holando de dos biotipos, Norteamericano (NA) y Neozelandés (NZ), bajo dos estrategias de alimentación (pastura o mixto). En la Unidad Experimental de Lechería de INIA La Estanzuela (Colonia, Uruguay) fueron agrupadas 120 vacas en 4 tratamientos según la combinación de biotipo y estrategia de alimentación (n=30). La cantidad de vacas con mastitis clínica y los RCS fueron registrados mensualmente durante una lactación. Hubo una interacción entre el biotipo y la paridad de las vacas (primíparas y multíparas) (P=0,04) para la frecuencia de mastitis. Vacas primíparas NA presentaron menor probabilidad de presentar mastitis clínica (ODR: 0,003). Por el contrario, las vacas multíparas NA fueron las que presentaron mayor probabilidad de experimentar mastitis clínica (ODR: 0,12), mientras que las vacas de origen NZ mostraron valores intermedios (ODR primíparas: 0,082; ODR multíparas: 0,066). La estrategia de alimentación no afectó la frecuencia de mastitis ni los RCS. Tampoco el biotipo afectó el RCS. En conclusión, sin importar la estrategia de alimentación, la frecuencia de mastitis clínica fue diferente según el biotipo, pero estas diferencias fueron influenciadas por el número de partos de los animales. Los RCS no fueron afectados por la estrategia de alimentación o el biotipo animal.

Outbreak investigation and control case report of brucellosis: experience from livestock research centre, Mpwapwa, Tanzania.

Brucellosis screening was conducted between 2005 and 2010 at the National Livestock Research Institute headquarters, Mpwapwa, Tanzania, following an abortion storm in cattle. The initial screening targeted breeding herds; 483 cattle were screened using the Rose Bengal Plate Test (RBPT) followed by the Competitive Enzyme-linked Immunosorbent Assay (c-ELISA) as a confirmatory test. The seropositivity on c-ELISA was 28.95% in 2005; it subsequently declined to 6.72%, 1.17%, 0.16% and 0.00% in 2006, 2007, 2009 and 2010, respectively. Brucella seropositivity was not detected in goats. Seropositivity declined following institution of stringent control measures that included: gradual culling of seropositive animals through slaughter; isolation and confinement of pregnant cows close to calving; proper disposal of placentas and aborted foetuses; the use of the S19 vaccine; and restricted introduction of new animals. It was thought that the source of this outbreak was likely to have been from the introduction of infected animals from another farm. Furthermore, humans were found with brucellosis antibodies. Out of 120 people screened, 12 (10%) were confirmed seropositive to brucella antigen exposure by c-ELISA analysis. The majority of the seropositive individuals (80%) were milkers and animal handlers from the farm. Nine individuals had clinical signs suggestive of brucellosis. All cases received medical attention from the district hospital. This achievement in livestock and human health showed that it is possible to control brucellosis in dairy farms, compared to pastoral and agro-pastoral farms, thus providing evidence to adopt these strategies in dairy farms thought to be at risk.

A SNP Harvester Analysis to Better Detect SNPs of CCDC158 Gene That Are Associated with Carcass Quality Traits in Hanwoo.

The purpose of this study was to investigate interaction effects of genes using a Harvester method. A sample of Korean cattle, Hanwoo (n = 476) was chosen from the National Livestock Research Institute of Korea that were sired by 50 Korean proven bulls. The steers were born between the spring of 1998 and the autumn of 2002 and reared under a progeny-testing program at the Daekwanryeong and Namwon branches of NLRI. The steers were slaughtered at approximately 24 months of age and carcass quality traits were measured. A SNP Harvester method was applied with a support vector machine (SVM) to detect significant SNPs in the CCDC158 gene and interaction effects between the SNPs that were associated with average daily gains, cold carcass weight, longissimus dorsi muscle area, and marbling scores. The statistical significance of the major SNP combinations was evaluated with x2-statistics. The genotype combinations of three SNPs, g.34425+102 A>T(AA), g.4102636T>G(GT), and g.11614+19G>T(GG) had a greater effect than the rest of SNP combinations, e.g. 0.82 vs. 0.75 kg, 343 vs. 314 kg, 80.4 vs 74.7 cm2, and 7.35 vs. 5.01, for the four respective traits (p<0.001). Also, the estimates were greater compared with single SNPs analyzed (the greatest estimates were 0.76 kg, 320 kg, 75.5 cm2, and 5.31, respectively). This result suggests that the SNP Harvester method is a good option when multiple SNPs and interaction effects are tested. The significant SNPs could be applied to improve meat quality of Hanwoo via marker-assisted selection.

A Whole Genome Association Study on Meat Quality Traits Using High Density SNP Chips in a Cross between Korean Native Pig and Landrace.

A whole genome association (WGA) study was performed to detect significant polymorphisms for meat quality traits in an F2 cross population (N = 478) that were generated with Korean native pig sires and Landrace dams in National Livestock Research Institute, Songwhan, Korea. The animals were genotyped using Illumina porcine 60k SNP beadchips, in which a set of 46,865 SNPs were available for the WGA analyses on ten carcass quality traits; live weight, crude protein, crude lipids, crude ash, water holding capacity, drip loss, shear force, CIE L, CIE a and CIE b. Phenotypes were regressed on additive and dominance effects for each SNP using a simple linear regression model, after adjusting for sex, sire and slaughter stage as fixed effects. With the significant SNPs for each trait (p<0.001), a stepwise regression procedure was applied to determine the best set of SNPs with the additive and/or dominance effects. A total of 106 SNPs, or quantitative trait loci (QTL) were detected, and about 32 to 66% of the total phenotypic variation was explained by the significant SNPs for each trait. The QTL were identified in most porcine chromosomes (SSCs), in which majority of the QTL were detected in SSCs 1, 2, 12, 13, 14 and 16. Several QTL clusters were identified on SSCs 12, 16 and 17, and a cluster of QTL influencing crude protein, crude lipid, drip loss, shear force, CIE a and CIE b were located between 20 and 29 Mb of SSC12. A pleiotropic QTL for drip loss, CIE L and CIE b was also detected on SSC16. These QTL need to be validated in commercial pig populations for genetic improvement in meat quality via marker-assisted selection.

Detection of Quantitative Trait Loci for Growth and Carcass Traits on BTA6 in a Hanwoo Population

The purpose of this study was to detect quantitative trait loci (QTL) for growth and carcass quality traits on BTA6 in a population of Hanwoo cattle. Three hundred and sixty one steers were produced from 39 sires that were sired by 17 grandsires in the two Hanwoo farming branches of the National Livestock Research Institute of Korea, between Spring 2000 and Fall 2002. DNA samples were collected for all of the steers, sires and grandsires, and the phenotypes for six growth and carcass quality traits were measured at 24 months of age. Twelve microsatellite markers were chosen on BTA6 and a linkage map was constructed by using seven of the twelve markers. Then, a chromosome-wide QTL scan was performed by applying an Animal Model, in which effects of QTL alleles within the grand sires were fitted as a random term. Three QTL were detected at the 5% chromosome-wise level for backfat thickness, average daily gain, and final weight. The most likely positions for the QTL were in the proximal region, i.e. 0 cM, 35 cM, and 63 cM, respectively. Also, another QTL for longissimus dorsi muscle area was detected at the 10% chromosome-wise level at 67 cM. These results were, in general, consistent with our previous report, in which candidate gene analyses showed that a SNP near ILSTS035 flanked by BM4621 (62.5 cM) and BMS2460 (81.3 cM) was associated with final weight, carcass weight, average daily gain, and longissimus dorsi muscle area in the same Hanwoo population.

한우의 이유전 성장형질에 관한 유전모수 추정

본 연구에서는 생산성이 인정된 모돈 F1(YL)에 종료웅돈(Terminal Sire)으로 Duroc종 이외에 생산성 저하에 많은 영향을 미치지 않을 것으로 판단되는 최종 종료웅돈(Terminal Sire) Berkshire종과 Berkshire×Duroc(F1)을 YL(♀) 교배시켜 생산된 비육돈(YLD, YLB, YLBD)의 성장과 육질을 비교 분석 하였다. 성장형질에서는 YL(♀)×BD(♂)가 YL(♀)×D(♂)보다 우수하였으며, 조사된 성장형질 전체에서 유의적인 차이(p<0.05)를 보였다. 육질에서도 pH, 보수력, 육색이 다른 비육돈 보다 유의적으로(p<0.05) 우수하였고, 돼지 육질의 표준이 되는 등심조지방 함량에서 YL(♀)×BD(♂)가 유의적으로 우수하였다. 교배조합별 육색에서는 YL(♀)×BD(♂)가 대체로 명도와 적색도가 높았으며, 지방산 조성에 있어서는 불포화 지방산의 비율이 높은 것으로 나타났다. 따라서, 성장형질에서는 YL(♀)×BD(♂)가 비교 대상의 교배조합에 비해 우수하였으며, 육질에서도 YL(♀)×D(♂) 보다 좋거나 같아 비육돈 교배조합(YLBD)으로서의 이용가치가 있을 것으로 사료된다. 하지만 본 연구의 경우 경남 첨단양돈연구소의 계통조성이 완료된 American Berkshire종을 이용하여 경남의 경우 경남 첨단양돈연구소를 활용하여 별도의 비용이 없이 YLBD를 생산이 가능하겠으나, 다른 지역의 경우 Berkshire의 계통 조성하여 활용할 경우 기존의 종료웅돈 Duroc종을 계통 조성하는 비용만큼 더 소요될 것이라 사료된다. A total of 4,627 records on pre-weaning growth traits, birth weight(BW), weaning weight(WW) and pre-weaning daily gain(ADG) of Hanwoo’s born form 1970 to 2005 were collected from Hanwoo Experiment Station, National Livestock Research Institute. Variance components and heritabilities of BW, WW and ADG were estimated with two models; Model 1 included only direct genetic effect while Mode1 2 included direct genetic, maternal genetic and permanent environmental effects. Direct herit- ability estimates of BW, WW and ADG estimated by model 1 were 0.34, 0.26 and 0.20, respectively. In model 2, direct heritability and maternal genetic heritability estimates were 0.14 and 0.06 for BW, 0.07 and 0.04 for WW and 0.05 and 0.04 for ADG. Estimates of genetic correlation between direct and maternal effect for BW, WW and ADG were 0.58, 0.75 and 0.61, respectively. Genetic correlation coefficients between BW and WW, between BW and ADG, and between WW and ADG were 0.76, 0.62 and 0.99, respectively.

오차드그라스 신품종 "코디원"의 생육특성과 수량성

신품종 "코디원"은 오차드그라스 계통들 중 생육특성 및 내재해성이 우수한 5계통을 집단 교배하여 생산된 합성종자를 2001년부터 2002년까지 수원에서 생산력 검정시험이 수행되었고, 2003년부터 2005년까지 3년동안 지역적응성 시험으로 수원, 남원, 대관령 및 제주지역에서 생육특성 및 수량성이 조사되었다. 코디원의 월동전 및 봄의 초형은 중간형이며, 엽은 농녹색이고, 출수기에 지엽의 길이는 짧으며, 상부절간장의 길이도 짧고 풍엽성이 양호하였다. 출수기는 5월 10일로 표준품종 Ambassador가 5월 8일로 2일이 늦었으며, 출수기에 초장은 71cm로서 Ambassador와 비슷하였다. 내한성과 도복은 코디원이 Ambassador에 비하여 강하였으며, 엽부병 및 녹병은 코디원이 Ambassador와 같이 강한 편이었다. 코디원의 ha당 건물수량은 12,081 kg으로 Ambassador 보다 11% 많았고, 모든 지역에서 증수되었다. 코디원의 사료 품질특성은 Ambassador와 비슷하였다. A new orchardgrass (Dactylis glomerata L.) variety named "Kordione" has developed by the National Livestock Research Institute (NLRI) in 2005. To develope a new variety of orchardgrass, 5 superior clones were selected and polycrossed for seed production. Various agronomic growth paramaters and forage production of "Kordione" were examined at Suwon from 2001 to 2002. During 2003-2005, regional trials were conducted at Suwon, Pyungchang, Youngju, Iksan, and Jeju. "Kordione" showed a medium type growth habit in fall and spring, and short in length of flag leaf and upper internode. Plant height of "Kordione" was similar to that of standard variety, "Ambassador" and heading date was 2 days later than 10th May compared to Ambassador. "Kordione" showed enhanced winter hardiness, lodging tolerance and disease resistance compared to Ambassador. In addition, dry matter yield (12,081 kg/ha) of "Kordione" was also higher (11%) than that of the Amabassador. However, nutritive value was similar in both varieties.

생육시기 및 품종이 총체 벼의 수량 및 사료가치에 미치는 영향

본 시험은 사료용 총체 벼의 수확시기 및 품종이 수량 및 사료가치에 미치는 영향을 구명하기 위하여 2003년부터 2005년까지 축산연구소에서 수행되었다. 식용벼인 추청벼와 총체벼 전용품종인 하마사리를 이용하여 생육시기별로 6회(출수, 개화, 유숙, 호숙, 황숙 및 완숙기)에 걸쳐 수확하였다. 조단백질 함량은 수확이 늦어짐에 따라 감소하였으나 TDN 함량은 증가되었다. ADF 함량으로 추정된(<TEX>$TDN=88.9-(0.79{\times}ADF)$</TEX>) 평균 TDN 함량은 60% 내외로 나타났다. ADF 및 NDF 함량은 수확이 지연됨에 따라 감소되었다. 건물수량은 황숙기에 가장 높았으며(p<0.05) 추청벼가 하마사리 보다 약간 높았지만 유의적이 차이는 없었다. TDN 수량도 건물수량과 같은 경향을 보였으나 매 수확시기별 품종간에는 유의적인 차이가 나타났다(p<0.05). 따라서 이상의 결과를 종합하여 볼 때 총체 벼의 수확적기는 호숙기<TEX>${\sim}$</TEX>황숙기로 추천되었다. This experiment was conducted to determine the effect of growth stage and variety on the yield and quality of whole crop rice (WCR) at National Livestock Research Institute, RDA from 2003 to 2005. Two types of rice ("Chucheong" for food and "Hamasari" for feed) were harvested at six different growth stages (heading, flowering, milk, dough, yellow ripening and fully ripening stage). Crude protein content decreased with progressed maturity at harvest. As growth stage delayed, TDN (total digestible nutrient) content of all rice varieties increased. The average TDN content of two type of rice was about 60% (59.3) which was calculated from ADF(acid detergent fiber) content. The contents of ADF and NDF(neutral detergent fiber) decreased with harvest maturity. The highest DM(dry matter) yield was at the ripening stage (p<0.05) and "Chucheong" gave higher yields than for "Hamasari". In this experiment, the DM yield of all varieties was low. The yield of TDN followed a similar trend to DM yield. According to this results that dough to yellow ripening stage of harvest maturity will be recommendable as proper harvest time for whole crop rice silage.

논에서 수수 X 수단그라스 교잡종 재배시 가축분뇨 이용이 생육특성, 수량, 사료가치 및 NO3-N의 용탈에 미치는 영향

본 시험은 논에서 가축분뇨를 활용하여 수수<TEX>${\times}$</TEX>수단그라스 교잡종 재배시 생육특성, 수량 및 토양중의 질산태 질소의 변화를 구명하기 위하여 2003년부터 2005년까지 3년간 축산연구소(수원) 포장에서 시험을 실시하였으며 그 결과를 요약하면 다음과 같다. 수수<TEX>${\times}$</TEX>수단그라스 교잡종의 생육특성 중 초기생육은 화학비료구 4.3에 비하여 가축분뇨를 시용한 경우 <TEX>$2.5{\sim}3.8$</TEX>로 양호하였고 1차 예취후 재생은 화학비료구 3.0에 비하여 돈분액비, 발효돈분구는 각각 2.8로 양호한 반면 발효우분구는 4.1로 불량하였다. 가축분뇨별 시용수준 간에는 가축분뇨 100% +화학비료 25%> 가축분뇨 75%+화학비료 25%> 가축분뇨 100% 순으로 생육이 양호하였다. 건물수량은 화학비료에 비하여 돈분액비 23%, 발효돈분 18% 증수되었으나, 발효우분구는 12% 감소되었다. 또한 TDN 수량도 같은 경향으로 돈분액비 시용구에서 24%, 발효돈분 시용시에 18% 증가되었으며 발효우분의 경우는 12% 감소되었다. 사료가치는 가축분뇨를 시용함으로 조단백질 함량이 화학비료 시용구 5.9% 보다 다소 낮아지는 경향으로 돈분액비 5.6%, 발효돈분 5.4%, 발효우분 5.5%이었다. 가축분뇨의 시용방법 간에는 일정한 경향이 나타내지 않았다. RFV에 있어서도 화학비료구가 81로 가장 높았으며, 가축분뇨를 시용한 구에서는 다소 낮아지는 경향으로 돈분액비 77, 발효돈분 77, 발효우분 79 이었다. <TEX>$NO_{3^-}N$</TEX> 용탈량(2년 평균)은 평균 돈분액비구 <TEX>$37.0mg/{\ell}$</TEX>, 발효 돈분구 <TEX>$32.9mg/{\ell}$</TEX>으로 많았으며 발효우분구는 <TEX>$17mg/{\ell}$</TEX>으로 적었다. 가축분뇨 시용수준 간에는 가축분뇨 100%+화학비료 25%>가축분뇨 75%+ 화학비료 25%>가축분뇨 100% 순이었으며 가축분뇨의 종류에 관계없이 시용 초기에 용탈량이 많았다. The experimental work was conducted to determine the growth characteristics, yield and feed value of sorghum-sudangrass hybrid and <TEX>$NO_{3^-}N$</TEX> leaching by application of various types of livestock manure (LM) at National Livestock Research Institute, Suwon, for 3years (2003-3005). The growth characteristics in chemical fertilizer (CF) was better than others in general. The growth characteristic of sorghum-sudangrass hybrid by the various type of LM was good in order of composted swine manure (CSM) > liquid swine manure (LSM) > composted cattle manure (CSM), whereas the growth characteristics by application level of LM was good in order of LM 100%+CF 25%>LM 75%+CF 25%>LM 100%. Dry matter(DM) yield in LSM and CSM increased by 23% and 18% respectively while DM yield in CCM decreased 24% as compared to CF. Moreover total digestible nutrients (TDN) in LSM and CSM increased by 24% and 18% respectively while TDN in CCM decreased 12% as compared to CF. Crude protein and relative feed value in LM decreased compared to those in CF. <TEX>$NO_{3^-}N$</TEX> leaching by application level of LM showed that there was an increase in order of LM 100%+ CF 25%>LM 75%+CF 25%>LM 100%. Also the high concentration of <TEX>$NO_{3^-}N$</TEX> occurred shortly after application of LM.

내한 다수성 이탈리안 라이그라스 신품종 '코위너'

본 연구는 이탈리안 라이그라스 내한성 품종 을 육성하기 위하여 1987년부터 2005년까지 농촌진흥청 축산연구소 조사료자원과에서 수행되었다. 신품종 '코위너'는 4배체 품종으로서 엽의 색은 농녹, 월동전 초형은 반포복형, 봄의 초형은 중간형이었다. '코위너'는 5월 19일경에 출수하는 중만생 품종으로 지엽의 길이가 29cm 로서 '화산 101호'와 같았으며 최장간장은 70cm로 '화산 101호' 보다 3cm 길었으며, 초장 은 97cm로 '화산 101호' 보다 3cm 길었다. 또한 '코위너'는 줄기 두께가 '화산 101호' 보다 두터웠으며, 1차 수확 후 재생이 양호하였다. '코위너' 품종의 내한성은 '화산 101호' 보다 다소 약하고 'Florida 80' 보다는 다소 강하였다. '코위너'의 건물수량은 14,345kg/ha로서 '화산 101호' 보다 8% 많았다. '코위너'의 사료가치는 in vitro 건물소화율(IVDMD)이 76.2%, 가소화양분총량(TDN)이 63.3%로서 '화산 101호' 보다 각각 1.6, 0.4% 낮았다. This experiment was carried out to breed the cold-tolerant variety of Italian ryegrass(Lolium multiflorum Lam.) in Grassland and Forages Division, National Livestock Research Institute, RDA, Suwon from 1987 to 2005. New variety, Kowinner as a tetraploid variety was dark green in leaf color and growth habit in autumn and early spring was semi-prostrate and medium, respectively Kowinner as a medium or late-heading variety was 19th May in heading date. Flag leaf length of Kowinner was 29 cm that was the same as control variety, Hwasan 101. Length of longest stem and plant length of Kowinner were 70 cm and 97 cm that was longer 3 cm than those of Hwasan 101, respectively. Stem width of Kowinner was thicker than that of Hwasan 101. Expecially, Kowinner was very good in regrowth. Cold tolerance of Kowinner was slightly weaker than that of Hwasan 101 and slightly stronger than that of Florida 80. Dry matter(DM) yield(12,884 kg/ha) of Kowinner was 8% more than that of Hwasan 101. In vitro dry matter digestibility and total digestible nutrient(TDN) of Kowinner were 76.2 and 63.3% that was 1.6 and 0.4% lower than those of Hwasan 101, respectively.

유산균제 첨가가 라운드베일 목초 사일리지의 품질에 미치는 영향

본 시험은 젖산균 첨가제가 목초 라운드베일 랩핑 사일리지의 품질에 미치는 영향을 구명하기 위하여 2년간 수행되었다. 시험구 배치는 난괴법 3반복으로 무처리, 첨가제 A, B, C로 하여 총 4처리를 두었다. 조지방, 조단백질, in vitro 건물 소화율은 첨가제를 처리한 구에서 높게 나타났다. 또한 첨가제 처리는 사일리지의 pH를 유의적으로 낮추었다(p<0.05). 건물 함량은 첨가제 처리구가 높았으며 첨가제 처리로 유기산 함량은 유의적인 차이를 보여주었다(p<0.05). 젖산 함량은 첨가제 처리로 증가되었으나 초산과 낙산 함량은 감소되었다. 건물손실과 사일리지 품질은 젖산균 첨가제 처리로 개선되는 효과를 보여 주었다. 이상의 결과를 종합하여 볼 때 사일리지 조제시 미생물 첨가제의 이용은 사일리지의 발효와 품질을 개선시켜주는 것으로 나타났다. This experiment was conducted to determine the effect of microbial inoculant on the quality of round baled grass silage at experimental field of Grassland and Forages Division, National Livestock Research Institute, RDA, Suwon from 1997 to 1998. The experiment was consist of randomized block design with three replications. The treatments were three different inoculant (control, inoculant A, B and C). The contents of ether extract(EE), crude protein(CP) and in vitro dry matter digestibility(IVDMD) in inoculant treatment plots were higher than those in control. Silages treated by Inoculant A, Band C had significantly lower acidity than that of control silage (p<0.05). Dry matter(DM) content of control silage was higher than those of inoculant treated silage. There were significant differences in organic acid contents among treatments(p<0.05). Lactic acid was increased with inoculant treatment, but, acetic and butyric acid was decreased. The DM loss of all silages were decreased with inoculant treatment and quality grade of inoculant treated silage was higher than that of control. Results of this study indicate that addition of microbial inoculant will improve the fermentation and quality of round baled grass silage.

셀레늄 급여원에 따른 한우 채끝육의 육질 특성

This study was conducted to investigate the feeding effect of selenium provided by different sources on the physico-chemical properties of M. longissimus thoracis. Twenty Hanwoo steers(20 24 months, approximately 613 kg) were assigned to 4 different feeding groups of each five, depending on the sources of selenium such as inorganic selenium(sodium selenite), organic selenium(Yeast, USA Sel-Plex), Se-SMC(Se-spent mushroom compost) and the control(no selenium). The selenium level in feed was 0.9 ppm for all groups except the control. Animals were fed with different feeding treatments for 12 weeks and slaughtered at National Livestock Research Institute. The inorganic selenium group had the highest cooking loss(CL. %) and followed by the Se-SMC group and organic selenium group. No significant differences in chemical composition, WB-shear forces, water-holding capacity(WHC) and sensory properties were found among the groups(P>0.05). There were 40% frequencies of grade A in the inorganic selenium group and followed by the organic selenium group(20%). Se-SMC group had 80% of frequencies for grade 1+ and followed by the inorganic selenium group(40%), whereas the organic selenium group and the control group had the 20% frequencies for grade 1+. From the results of this study showed that the selenium sources did not affect chemical composition, WHC and tenderness of Hanwoo steers. However, the inorganic selenium group had the highest cooking loss(%) and lowest pH when compared to the other groups.

홀스타인 착유우의 착유시간, 착유속도 및 착유량에 대한 유전모수 추정

본 연구는 우군의 일일 유량에 대한 비유형질의 특성을 분석하고자 하였으며 공시된 젖소는 농촌진흥청 축산연구소에서 사육된 착유우로서 2005년 11월부터 2006년 4월까지 1일 2회 착유를 하는 우군의 자료를 이용하였다. 시험축은 하나의 우군으로 동일한 사양조건에 의하여 관리된 종모우 66두를 가지는 착유우 154두에서 수집된 기록 12,561개를 분석하였다. 공시축의 평균유기는 139.7일, 평균산차는 1.9산, 착유당 평균유량 13kg을 나타내었다. 평균 비유속도는 최고 비유속도의 64% 수준으로 측정되었다. 비유지속시간, 최고비유속도, 평균비유속도, 착유당 평균유량에 대하여 오후 착유 보다 오전 착유가 높게 나타났고, 산차간에서는 비유지속시간을 제외하고 산차증가와 함께 증가하는 경향이었다. 비유지속시간, 최고비유속도, 평균비유속도 및 착유당 평균 유량에 대한 유전력은 각 0.49, 0.70, 0.58, 0.36으로 비교적 높게 추정되었다. 한편 비유지속시간과 비유속도간에는 부의 유전상관이, 비유지속시간과 착유당 유량간에는 정의 상관이, 최고비유속도에 대한 평균비유 속도(0.87)와 착유당 유량(0.23)은 정의 상관을 보였고, 평균비유속도와 착유당 유량간(0.30)에도 정의 유전상관을 보였다. 결론적으로 비유속도는 유량과 함께 증가하였으나 비유지속시간과는 부의 관계를 보였으나 보다 의미 있는 유전적 특성을 파악하기 위하여 더 많은 두수를 이용한 연구가 필요할 것으로 사료된다. This study estimated characteristics and genetic parameters for milking traits in Holstein dairy cattle. Daily records (12,561) were available from a hundred fifty four lactating cows with sixty six sires at National Livestock Research Institute from November, 2005 to April, 2006. The cows were fed same rations, and were milked twice daily using milking parlour equipped with Alpro-system?? to collect milking records automatically. The experimental cows have averaged 1.9 parities, 139.7 days in milk, and 13kg milk yield per milking. Average milk flow was 64% of peak milk flow. Milking duration, peak milk flow, average milk flow, and milk yield per milking were 5.57±1.67 minutes, 3.58±0.79kg/minute, 2.28±0.51kg/minute, and 13.02±4.09kg, respectively. All traits in the morning milking were higher than those of in the evening milking, and were also increased with parity. Heritabilities for milking duration, peak and average milk flow, and milk yield per time were 0.49, 0.70 0.58 and 0.36, respectively. The genetic correlations of milking duration with peak milk flow, average milk flow, and milk yield per time were 0.48, 0.54, and 0.41, respectively. The correlations of milk yield per milking with peak milk flow and average milk flow were 0.23 and 0.30, respectively. In conclusion, milk flow was increased with milk yield; however milk flow was opposite relationship with milk duration. Further studies are not only necessary to warranty genetic parameters, but pre-installation of automatic recording system to collect daily milking record is also necessary.

Festulolium braunii 교잡종의 재배특성, 사료가치 및 수량성

새로 도입되는 목초 Festulolium pratensis <TEX>${\times}$</TEX> Lolium muitiforum의 속간 교잡종인 Festulolium braunii의 내한성, 출수기, 사료가치 및 수량 등을 구명하여 우리나라 재배 가능성을 검토하고 자, 2004년 9월부터 2005년 7월까지 수원 및 남원에 있는 농촌진흥청 축산연구소 시험포장에서 동시에 수행한 연구 결과는 다음과 같다. Festulolium braunii 교잡종 Fleurial, Boxer 및 Perun 등 3개 교잡종의 내한성은 수원과 남원에서 화산101호 보다 강한 것으로 나타나, 국내 재배시 내한성의 문제는 거의 없을 것으로 보인다. 3개 교잡종의 평균 출수기는 남원에서 5월 25일, 수원에서 5월 27일로서, 화산101호 보다 <TEX>$1{\sim}3$</TEX>일 늦은 중만숙이었으며, 초장은 1차 수확기에 <TEX>$91{\sim}96cm$</TEX>, 2차에는 <TEX>$93{\sim}87cm$</TEX>로 비교적 긴 편이며, 도복도 강하였으며, 재생력도 우수하였다. ha당 청예 총 수량은 지역간, 교잡종간 유의적 차이가 인정되었으며, 수원의 평균 수량은 83,222kg, 남원은 69,139kg으로 수원에서 수량성이 높았으며, 2개 지역의 평균 수량에서도 화산101호의 64,944.kg에 비하여 Perun 은 129%로 가장 많이 증수하였으며 Fleurial, Boxer도 <TEX>$123{\sim}l17%$</TEX> 각각 증수 되었다. 그리고, 건물수량은 지역간에는 차이가 인정되지 않았으나 교잡종간에는 화산101호의 10,589kg에 비하여 Perun, Fleurial 및 Boxer가 각각 16,941 kg, 16,519kg 및 15,826kg으로 <TEX>$149{\sim}160%$</TEX>까지 증수되었다. 3개 교잡종들의 조성분 및 사료가치에서, 조단백질 함량은 Perun 18.0%, Fleuria 17.4% 및 Boxer 16.7%로 비교적 높게 나타났으며, 조지방 함량은 다소 높으면서 조섬유 함량 역시 낮으나 회분 함량은 다소 높은 편이며 소화율도 매우 높게 나타났는데, 이에 대한 연차간 변이가 있는지를 향후 구명되어져야 한다. 사료가치에서 ADF는 화산101호의 33.2%보다 더 낮은 <TEX>$28.1{\sim}29.2%$</TEX>이었으며, NDF는 화산101호의 58.3 보다 다소 적은 <TEX>$51.3{\sim}54.5%$</TEX>로 나타나므로써 이들 교잡종들의 사료품질 및 가치가 매우 높았음을 알 수 있다. This experiment was carried out in order to examine the winterhardiness, heading date, fresh and dry matter yeild of Festulolium braunii, a hybrid of Festuca pratensis and Lolium multiflum, which is newly introduced in Korea. This experiment was performed at National Livestock Research Institute in Suwon and Namwon from September 2004 to July 2005. The results are as follows. 'Fleurial', 'Boxer', and 'Perun' showed stronger winterhardiness than 'Hwasan 101', which is one of the strongest in the winterhardiness hybrids. Therefore, we do not see there is any problem in winterhardiness when these hybrids are cultivated in Korea. The hybrids are turned out to be mid and late maturing hybrid. The average heading dates of the three hybrids were May 25 in Namwon and May 27 in Suwon. These dates are lower(<TEX>$1{\sim}3$</TEX> days) than that of Hwasan 101. They showed <TEX>$91{\sim}96cm$</TEX> in leaf length at 1st cutting times which are comparatively long. This experiment also disclosed that their lodging and regrowth are strong and excellent. We also found out that there are significant difference between regions and hybrids in the total fresh yield per ha. The average fresh yield of Suwon was 83,222 kg/ha and that of Namwon was 69,139 kg/ha. We could not see any difference in the dry matter yield affected by region, however, there were significant difference in hybrids(p<0.05). The crude protein contents of the three hybrids indicated very high figures. the crude protein cotents of Perun, Fleurial and Boxer are 18.0% 17.4% and 16.7% respectively. However the ADF (Acid Detergent Fiber) shows <TEX>$28.1{\sim}29.2%$</TEX> which is less contents than 33.2% of Hwasan 101. We found out from this experiment that these three hybrids have excellent feed quality because the NDF (Neutral Detergent Fiber) is decreased by <TEX>$7{\sim}3.8%$</TEX> comparing that the NDF of Hwasan 101 is 58.3%. Also each hybrids contains more crude fat by 1%, a little more chemical composition and less ash contents. Thus we can tern that chemical composition contents of these hybrids are also fittable.

Mapping of the Porcine Calpastatin Gene and Association Study of Its Variance with Economic Traits in Pigs

The objectives of this study were to confirm a location of the calpastatin (CAST) gene in chromosome 2 and to detect associations of genetic variations with economic traits in the porcine CAST gene as a candidate gene for growth and meat quality traits in pigs. Calpastatin is a specific endogenous inhibitor of calpains. The calpain protease system is ubiquitous, and is involved in numerous growth and metabolic processes. Three single nucleotide variations were identified within a 1.6 kb fragment of the porcine CAST gene and these polymorphisms were used for genetic linkage mapping. Linkage and QTL mapping were performed with the National Livestock Research Institute (NLRI) reference families using eight microsatellites and SNP makers in the CAST gene. The porcine CAST gene was mapped adjacent to the markers, SW395 and SW1695 on SSC2 with LOD scores of 15.32 and 8.50, respectively. According to the QTL mapping, a significant association was detected at 82 cM between SW395 and CAST-Hinf I for weight at the age of 30 weeks. In addition, an association study was performed with the F2 animals of NLRI reference families for Hinf I, Msp I and Rsa I polymorphisms in the CAST gene. Two polymorphisms, CAST-Rsa I and CAST-Hinf I, showed significant correlation for growth traits at p<0.01 and p<0.05, respectively. (Key Words : Calpastatin (CAST), Linkage Mapping, QTL Mapping, Korean Native Pig)

Confirming Single Nucleotide Polymorphisms from Expressed Sequence Tag Datasets Derived from Three Cattle cDNA Libraries

Using the Phred/Phrap/Polyphred/Consed pipeline established in the National Livestock Research Institute of Korea, we predicted candidate coding single nucleotide polymorphisms (cSNPs) from 7,600 expressed sequence tags (ESTs) derived from three cDNA libraries (liver, M. longissimus dorsi, and intermuscular fat) of Hanwoo (Korean native cattle) steers. From the 7,600 ESTs, 829 contigs comprising more than two EST reads were assembled using the Phrap assembler. Based on the contig analysis, 201 candidate cSNPs were identified in 129 contigs, in which transitions (69%) outnumbered transversions (31%). To verify whether the predicted cSNPs are real, 17 SNPs involved in lipid and energy metabolism were selected from the ESTs. Twelve of these were confirmed to be real while five were identified as artifacts, possibly due to expressed sequence tag sequence error. Further analysis of the 12 verified cSNPs was performed using the program BLASTX. Five were identified as nonsynonymous cSNPs, five were synonymous cSNPs, and two SNPs were located in 3'-UTRs. Our data indicated that a relatively high SNP prediction rate (71%) from a large EST database could produce abundant cSNPs rapidly, which can be used as valuable genetic markers in cattle.

내한 조숙성 이탈리안 라이그라스 신품종 '코그린'

본 시험은 이탈리안 라이그라스 내한성 품종을 육성하기 위하여 1999년부터 2005년까지 축산연구소 조사료자원과에서 수행되었다. 이탈리안 라이그라스 신품종 '코그린'은 2배체 품종으로서 엽의 색은 녹이며, 월동전 초형은 반직립형, 봄의 초형은 직립형이다. 코그린은 5월 4일경에 출수하는 조숙성 품종이었다. 특히, '코그린'은 'Florida 80' 보다 지엽의 폭이 0.7mm, 지엽의 길이가 2cm로 넓고 길며, 초장은 85m로 Florida 80 보다 1cm 정도 작았다. 줄기의 두께는 'Florida 80'과 같이 중간이었다. '코그린'의 내한성은 'Florida 80' 보다 강한 품종이었다. '코그린'의 건물수량은 12.884kg/ha로서 'Florida 80' 보다 3% 적었다. '코그린'의 사료가치는 in vitro 소화율이 74.7%, 가소화영양분총량(TDN) of 65.1%로서 'Florida 80' 보다 각각 3.6, 1.5% 높았고, 산성세제불용섬유(ADF)와 중성세제불용섬유(NDF)는 30.1 및 54.1%로서 'Florida 80' 보다 각각 1.9 및 1.3% 낮았다. This experiment was carried out to breed the cold-tolerant variety of Italian ryegrass (Lolium multiflorum Lam.) at the Grassland and Forage Crops Division, National Livestock Research Institute, RDA at Suwon, Korea, from 1999 to 2005. The new variety 'Kogreen' is a diploid variety with a green leaf color and had semi-erect habit in late autumn and erect growth habit in early spring. The heading date of 'Kogreen' was on May 4, and for 'Florida 80' was May 2, both are considered as early-heading variety. 'Kogreen' was wider by 0.7mm in flag leaf width, longer by 2 cm in flag leaf length and shorter by 1cm in plant length than the control variety, Florida 80. Stem width of 'Kogreen' was similar to that of 'Florida 80' and 'Hwasan 101'. cold tolerance of 'Kogreen' was better than that of 'Florida 80'. Dry matter(DM) yield (12,884kg/ha) of 'Kogreen' was 3% less than that of 'Florida 80'. in vitro dry matter digestibility was 74.7% and total digestible nutrient was 65.1%, which are 3.6% and 1.5% higher than those of 'Florida 80', respectively. Acid detergent fiber and neutral detergent fiber of 'Kogreen' were 30.1% and 54.1% which are 1.9% and 1.3% lower than those of 'Florida 80', respectively.

예건기간이 라운드베일 목초 사일리지의 품질에 미치는 영향

본 시험은 예건기간이 목초 라운드베일 사일리지의 품질에 미치는 영향을 구명하기 위하여 축산연구소에서 2년간 수행되었다. 사일리지 조제전 목초를 수확하여 비예건, 반나절 예건 및 1일 예건 등 3처리를 두고 각각 3롤의 라운드베일 사일리지를 조제하였다. 일반성분중 조섬유 함량은 예건으로 증가되었으나 나머지 성분은 일정한 경향을 보이지 않았다. 섬유소 함량에 있어서도 유의적인 차이는 없었으나 건물 소화율에 있어서는 1일 예건구가 비예건구에 비해 유의적으로 낮았다. 건물함량은 예건기간이 길어짐에 따라 23.7%에서 40.0%로 높아졌으며 pH도 증가되었다. 사일리지의 유기산 함량은 예건기간이 길어질수록 감소되는 경향을 보여주었으며 사일리지의 품질 점수 및 등급이 약간 개선되었다. 건물 손실에 있어서는 예건으로 약 <TEX>$4{\sim}10%$</TEX> 줄일 수 있었다. 이상의 결과를 종합하여 볼 때 고품질의 목초 라운드베일 사일리지 조제를 위해서는 1일정도 예건하는 것이 권장되었다. This experiment was conducted to determine the effect of management practices on the qualify of round baled grass silage at experimental field of grassland and Forage Crops division, National Livestock Research Institute, RDA, Suwon from 1997 to 1998. The experiment was consisted of three different wilting days : 0 (unwitted), 0.5 and 1 day with three replications. Crude fiber content was increased with prolonged wilting period, but other components did not show any trends. There was no significant differences in acid detergent fiber (ADF) and neutral detergent fiber (NDF) content between wilting periods, but there was significant difference in IVDMD (in vitro dry matter digestibility) between one-day wilted and unwitted silage. Dry matter (DM) content changed from 23.7% to 40.5% in relation to prolonged wilting period. Wilting treatment caused a decrease in each organic acid content of round baled grass silage and an increase in the silage quality. Wilting also could reduce the DM loss. This study demonstrated that one-day wilting would be a recommendable method for making high quality round baled grass silage.

한우 암소의 성장곡선 모수에 대한 유전적 경향

The objective of this study was to estimate genetic variances of growth curve parameters in Hanwoo cows. The data used in this study were records from 1,083 Hanwoo cows raised at Hanwoo Experiment Station, National Livestock Research Institute (NLRI). First evaluation model (Model I) fit year-season of birth and age of dam as fixed effects and second model (Model II) added age at the final weight as a linear covariate to Model I. Heritability estimates of A, b and k from Gompertz model were 0.22, 0.11 and 0.07 using model and 0.28, 0.11 and 0.12 using model . Those from Von Bertalanffy model were 0.22, 0.11 and 0.07 using model , 0.28, 0.11 and 0.12 using model . Heritability estimates of A, b and k from Logistic model were 0.14, 0.07 and 0.05 using model , 0.18, 0.07 and 0.12 using model . Heritability estimates of A from Gompertz model were higher than those from Von Bertalanffy model or Logistic model in both model and model . Heritability estimates of b from Logistic model were higher than those from Gompertz model or Von Bertalanffy model in both model and model . Heritability estimates of birth weight, weaning weight, 3 month weight, 6 month weight, 9 month weight, 12 month weight, 18 month weight, 24 month weight, 36 month weight were after linear age adjustment 0.27, 0.11, 0.19, 0.14, 0.16, 0.23, 0.52 and 0.32, respectively. Heritability estimates of birth weight, weaning weight, 3 month weight, 6 month weight, 9 month weight and 24 month weight fit by Gompertz model were larger than those estimated from linearly adjusted data. Heritability estimates of 12 month weight, 18 month weight and 36 month weight fit by Von Bertalanffy model were larger than those estimated from linearly adjusted data. In the multitrait analyses for parameters from Gompertz model, genetic and phenotypic correlations between A and k parameters were 0.47 and 0.67 using model and 0.56 and 0.63 using model . Those between the A and b parameters were 0.69 and 0.34 using model and 0.72 and 0.37 using model . Those between the b and k parameters were 0.26 and 0.01 using model and 0.30 and 0.01 using model . In the multitrait analyses for parameters from Von Bertalanffy model, genetic and phenotypic correlations between A and k parameters were 0.49 and 0.67 suing model and 0.57 and 0.70 using model . Those between the A and b parameters were 0.61 and 0.33 using model and 0.60 and 0.30 using model . Those between the b and k parameters were 0.20 and 0.02 using model and 0.16 and 0.00 using model . In the multitrait analyses for parameters from Logistic model, genetic and phenotypic correlations between A and k parameters were 0.43 and 0.67 using model and 0.50 and 0.63 using model . Those between the A and b parameters were 0.47 and 0.22 using model and 0.38 and 0.24 using model . Those between the b and k parameters were 0.09 and 0.02 using model and 0.02 and 0.13 using model .

Estimation of Genetic Parameters for Economic Traits in Korean Native Chickens

Heritabilities and genetic correlations of economic traits were estimated with a multiple-trait animal model on 5 different strains of Korea Native Chickens (KNC): Red Brown (RB), Yellow Brown (YB), Gray Brown (GB), Black (B) and White (W). The data used for this study were collected from the Daejeon branch of the National Livestock Research Institute and included 11,233 performance records and 12,729 individual animals. DFREML and SAS BASE/STAT packages were used to estimate genetic parameters and descriptive statistics. The estimated heritabilities for strain RB, YB, GB, B and W, respectively, for age at 1st egg were 0.24, 0.27, 0.12, 0.32 and 0.18; for body weight at first egg were 0.39, 0.43, 0.38, 0.52 and 0.57; for body weight at age of 270 days were 0.43, 0.51, 0.30, 0.52 and 0.67; for egg weight at first egg were 0.08, 0.13, 0.07, 0.06 and 0.07; for egg weight at age of 270 days were 0.37, 0.43, 0.22, 0.34 and 0.41; and for number of eggs laid by age of 270 days were 0.24, 0.25, 0.37, 0.36 and 0.30. The genetic and phenotypic correlations were also estimated.