MXenes have emerged as a fascinating material for RT gas-sensing applications due to their outstanding properties. This work reports the design and fabrication of a high-performance ammonia sensor based on MXenes material. To improve its sensing performance, a MXenes nanosheets thin film with a thickness of ≈ 10 nm was prepared using a scalable self-assembled method. Specifically, we employed the controlled and enhanced interfacial self-assembled method to fabricate a continuous and conductive MXene ultra-thin film. The morphology and the structure of the produced MXene materials and their films were characterized by combining multi-characterization tools. Altoghether, these highlight the uniform and continuous deposition of the MXene nanosheets film on the glass substrate. The electrical characterization indicates a sheet resistance value of 4.82 × 105 Ω/sq. The fabricated devices present good RT sensing performances for NH3 detection, ranging from 1 to 20 ppm. This demonstrates the outstanding sensitivity of MXene with a value of 1.92 % for the lowest concentration (1 ppm), a fast response (179 s) and a recovery time of (612 s), which is related to the high quality of the ultra-thin MXene sensiting layer. Moreover, the sensors demonstrate high degree of stability and excellent reproducibility, make them suitable candidate for practical applications like environmental monitoring.