Nanometer Level Research Articles

Controlled layer-by-layer assembly and structured coloration of Ti3C2Tz MXene/polyelectrolyte heterostructures

Structural color arises from light scattering rather than organic pigments and can be found in Nature, such as in bird feathers and butterfly wings. Synthetic materials can mimic Nature by leveraging materials with contrasting optical characteristics by controlling each materials’ spatial arrangement in a heterostructure. Two-dimensional MXene nanosheets are particularly interesting due to their unique optical properties, but MXenes have not been used directly as a structural colorant because it is challenging to control the spatial placement of MXenes at the nanometer level. Here, we report the emergence of structural color in layer-by-layer (LbL) assemblies of Ti3C2Tz MXene nanosheets and polyelectrolyte heterostructures with controlled block thicknesses. The block thickness and spatial placement of MXene are controlled by the assembly’s salt concentration and number of layer pairs. This work demonstrates that optical characteristics of MXene/polyelectrolyte heterostructures depend on MXene content and placement, while deepening the understanding of MXenes within structural color films.

Atomistic insights into salicylic acid treatment on human keratinized skin by XPS combined with argon gas cluster sputtering: A pilot study

Stratum corneum is the skin's outermost layer, whose main constituent is keratin. The treatment of dermatological diseases showing hyperkeratosis is a serious problem in dermatology and cosmetics seriously affecting the population showing increasing prevalence. One of the treatment is a peeling agent like salicylic acid (SA), categorized as a keratolytic agent, however its mode of action is not fully understood. X-ray photoelectron spectroscopy (XPS) is a method for monitoring the changes of surface composition at atomistic level in 5–10 nm depth. The development of argon cluster sputtering enabled the investigation of deeper regions (nanometric level) even for biomaterials. In this pilot study, the mode of action of SA has been studied at atomistic level for the first time. Different concentrations of SA (3, 5, 10 wt%) in different time frames (1 day, 1 week, 1 month) was applied on human keratinized skin. The carbon, nitrogen and oxygen peak have not shown changes during the treatment, not showing the suggested desmolytic mechanism of SA. However, significant changes were observed in the sulfur peak, with increasing time and concentration the rate of sulfonate and sulfate was increased compared to thiol group indicating the break of sulfide bonds between the polypeptide chains in keratin, indicating that keratolysis happens at nanometric level. Already a 1 week-10 wt% SA treatment resulted in higher rate of sulfate compared to sulfonate indicating further oxidation of the keratolytic product. However, 1 month-10 wt% treatment resulted in the same surface composition as 3 wt%-1-month treatment indicating that enough time with low concentration can exert the same effect as higher concentrations. These findings have relevance in the design of future SA-based dermatologic therapies.

Harnessing Plasmonic Interference for Nanoscale Ultrafast Electron Sources.

In this Letter we demonstrate the use of plasmonic focusing in conjunction with nonlinear photoemission to develop geometrically flat nanoscale electron sources with less than 40pm-rad root mean squared (rms) normalized transverse emittance. Circularly polarized light is incident on a gold Archimedean spiral structure to generate surface-plasmon polaritons that interfere coherently at the center resulting in a 50nm rms emission area. Such a nanostructured flat surface enables simultaneous spatiotemporal confinement of emitted electrons at the nanometer and femtosecond level and can be used as an advanced electron source for high-repetition-rate ultrafast electron diffraction and microscopy experiments as well as the next generation of miniaturized particle accelerators.

Improving strength-conductivity synergy of Al-Cu-(Sn/Er) alloy with nanoscale substructure

As a conductive material for power transmission, Al-Cu alloy is widely used in power engineering, microelectronics and other fields. In view of the contradiction between the conductivity and strength of aluminum alloy, Sn and Er elements were selected to micro-alloy Al-Cu alloy. Al-Cu, Al-Cu-Sn and Al-Cu-Sn-Er alloys were prepared by near-liquidus casting. After solution treatment, it was subjected to three-stage alternating process of rolling and aging. The effects of micro-alloying and alternating process on the microstructure and properties of Al-Cu alloy were studied. The results show that the precipitated phase Al2Cu of Al-1.12 Cu alloy is rod-shaped after the first-stage process, and the average size is about 278±87 nm. The precipitated phases in the Al-Cu-Sn alloy are Al2Cu (average size of about 206±79 nm) and nano-sized β-Sn particles. The fine β-Sn nanoparticles in the Al-Cu-Sn-Er alloy heterogeneously nucleated on Al8Cu4Er (average size of about 167±45 nm), making Al8Cu4Er grow into particles. After secondary processing, stacking faults (SFs) appeared in the substructures of the three alloys, and the precipitated phases were further refined. The size of most precipitated phases in Al-1.13Cu-0.12Sn and Al-1.01Cu-0.15Sn-0.22Er alloys is reduced to nanometer level. After the three-stage process, some of the Al2Cu precipitates in the Al-Cu alloy are also refined to the nanometer level, and the precipitates in the other two alloys are all refined to the nanometer level. Nanotwins also appear in Al-1.01Cu-0.15Sn-0.22Er alloy. The tensile strength of Al-1.13Cu-0.12Sn and Al-1.01Cu-0.15Sn-0.22Er alloys after three-stage alternating process is 303 MPa and 327 MPa, respectively. The elongations are 21.6 % and 23.3 %, respectively. The relative conductivity was 61.7 %IACS and 59.8 %IACS, respectively. The synergistic effect of microalloying of Sn and Er elements and three-stage alternating processing technology not only reduces the size of precipitates and twins in the alloy to the nanometer level, but also adjusts the distribution of dislocation tangles and stacking faults (SFs), improves the substructure configuration, and improves the mechanical and electrical properties of conductive aluminum alloys.

A Molecular View into the Structure and Dynamics of Phase-Separated Chromatin.

The organization of chromatin is critical for gene expression, yet the underlying mechanisms responsible for this organization remain unclear. Recent work has suggested that phase separation might play an important role in chromatin organization, yet the molecular forces that drive chromatin phase separation are poorly understood. In this work we interrogate a molecular model of chromatin to quantify the driving forces and thermodynamics of chromatin phase separation. By leveraging a multiscale approach, our molecular model is able to reproduce chromatin's chemical and structural details at the level of a few nanometers, yet remain efficient enough to simulate chromatin phase separation across 100 nm length scales. We first demonstrate that our model can reproduce key experiments of phase separating nucleosomal arrays, and then apply our model to quantify the interactions that drive their formation into chromatin condensates with either liquid- or solid-like material properties. We next use our model to characterize the molecular structure within chromatin condensates and find that this structure is irregularly ordered and is inconsistent with existing 30 nm fiber models. Lastly we examine how post-translational modifications can modulate chromatin phase separation and how the acetylation of chromatin can lead to chromatin decompaction while still preserving phase separation. Taken together, our work provides a molecular view into the structure and dynamics of phase-separated chromatin and provides new insights into how phase separation might manifest in the nucleus of living cells.

Optical properties and energy transfer of Tm3+ single-doped and Tm3+-Tb3+ co-doped transparent glass-ceramics containing NaGd(MoO4)2 single phase

Tm3+ single-doped and Tm3+-Tb3+ co-doped SiO2–B2O3–Na2O–ZnO–MoO3-Gd2O3 glasses were successfully prepared by the melt-quenching process. X-ray diffraction and transmittance measurements were used to determine that the optimal heat treatment regime consisted of crystallization at 620 °C for 5 h and transparent glass-ceramics containing the NaGd(MoO4)2 single phase were obtained. The characterization results by SEM confirmed that the size of the crystals precipitated in the glass matrix was on the nanometer level. The effects of different contents of Tm2O3 on the luminescent properties of glass-ceramics in this system were explored. The results showed that the emission peak at 453 nm (1D2→3F4) had the highest intensity when Tm2O3 was doped with 0.5 mol% under the wavelength at 359 nm excitation. In addition, with the gradual increase of Tb2O3 contents, the luminescence colour of the Tm2O3–Tb2O3 co-doped glass-ceramics gradually shifted from blue to green. This indicates its potential application in blue-green light display technologies and various other areas. The experimental results of the water resistance stability of the samples show that the co-doped glass-ceramic still has good luminescence stability after being immersed in water, which provides further favourable support for its application.

F-Bypass: a low-power network-on-chip design utilizing bypass to improve network connectivity

With the development of transistor feature size to nanometer level, static power consumption has gradually become the main factor affecting the overall power consumption of network on chip (NoC). Power gating is an effective technology to reduce static power consumption, but it also brings new challenges, such as BET violation, wake-up latency and network connectivity. Therefore, a power gating method is needed to improve NoC performance and reduce static power consumption. This paper proposes a low-power bypass method, namely Forwarding bypass (F-Bypass). First, F-Bypass adds bypass paths between all input and output ports and the network interface (NI), and connects the pop-up port and injection port in NI through the bypass path. When the router is powered off, F-Bypass performs wake-up-free packet transmission, which reduces the break-even time (BET) violation and cumulative wake-up latency while ensuring network connectivity. Secondly, This paper add the modified VC state table to NI, so that the power-off router can perform normal traffic control. Finally, a new wake-up criterion is proposed, which can effectively avoid the frequent wake-up of power-off routers, and the detailed hardware implementation of F-Bypass is provided.The simulation results under integrated traffic load show that compared with the traditional scheme, the delay of F-Bypass is reduced by 2.2%, the throughput is increased by 13.1%, and the total static power consumption is reduced by 75.2%. Key performance indicators are superior to other solutions, and the increased area cost is moderate.

Gesture recognition with Brownian reservoir computing using geometrically confined skyrmion dynamics

Physical reservoir computing leverages the dynamical properties of complex physical systems to process information efficiently, significantly reducing training efforts and energy consumption. Magnetic skyrmions, topological spin textures, are promising candidates for reservoir computing systems due to their enhanced stability, non-linear interactions and low-power manipulation. Traditional spin-based reservoir computing has been limited to quasi-static detection or real-world data must be rescaled to the intrinsic timescale of the reservoir. We address this challenge by time-multiplexed skyrmion reservoir computing, that allows for aligning the reservoir’s intrinsic timescales to real-world temporal patterns. Using millisecond-scale hand gestures recorded with Range-Doppler radar, we feed voltage excitations directly into our device and detect the skyrmion trajectory evolution. This method scales down to the nanometer level and demonstrates competitive or superior performance compared to energy-intensive software-based neural networks. Our hardware approach’s key advantage is its ability to integrate sensor data in real-time without temporal rescaling, enabling numerous applications.

Active illumination mode with checkerboard pattern in focus variation microscopy: Analysis and application

Optical measurement methods for surface topography offer the advantages of high accuracy, rapid measurement, and non-destructiveness. Each method has its own suitable application scenarios. Among them, focus variation microscopy is extensively employed in precision manufacturing, aerospace, and medical industries due to its ability to measure rough and large slopes surfaces. However, since the measurement depends on local grayscale differences between focused and blurred images, it cannot measure surfaces with low reflectivity or insufficient texture information. In this work, we propose an active illumination mode for focus variation method that utilizes a digital micromirror device (DMD) to generate a checkerboard pattern. This method introduces additional texture information, resulting in a usable local gradient of image grayscale. Additionally, we analyze the selection criteria for the checkerboard pattern parameters, including the period and light-dark ratio. Furthermore, measurements of two standard steps with different heights demonstrate that the measurement repeatability of the proposed method can reach the nanometer level, rendering it suitable for high-precision measurements. More importantly, the measurement noise results indicate significantly superior performance of active illumination mode compared to the uniform illumination mode. Finally, we reconstruct the surface topography of the microchannels in a microfluidic chip through the encapsulation layer, demonstrating the feasibility of the proposed method.

Visualizing plasmons and ultrafast kinetic instabilities in laser-driven solids using X-ray scattering

Ultra-intense lasers that ionize atoms and accelerate electrons in solids to near the speed of light can lead to kinetic instabilities that alter the laser absorption and subsequent electron transport, isochoric heating, and ion acceleration. These instabilities can be difficult to characterize, but X-ray scattering at keV photon energies allows for their visualization with femtosecond temporal resolution on the few nanometer mesoscale. Here, we perform such experiment on laser-driven flat silicon membranes that shows the development of structure with a dominant scale of 60 nm in the plane of the laser axis and laser polarization, and 95 nm in the vertical direction with a growth rate faster than 0.1 fs−1. Combining the XFEL experiments with simulations provides a complete picture of the structural evolution of ultra-fast laser-induced plasma density development, indicating the excitation of plasmons and a filamentation instability. Particle-in-cell simulations confirm that these signals are due to an oblique two-stream filamentation instability. These findings provide new insight into ultra-fast instability and heating processes in solids under extreme conditions at the nanometer level with possible implications for laser particle acceleration, inertial confinement fusion, and laboratory astrophysics.

Investigation of transverse exchange-springs in electrodeposited nano-heterostructured films through first-order reversal curve analysis

The prerequisite of efficient exchange-spring nano-heterostructures, i.e., tuning both hard and soft phases at a nanometer level, has posed significant preparation challenges to ensure effective exchange-coupling. Here, we present a novel approach to fabricate transverse exchange-spring nano-heterostructures using single starting material through an “in situ” electrodeposition technique at room temperature. Utilizing modified acidic bath chemistry and controlled hydrogen evolution, we successfully prepared stress-free, shiny, fine-grained amorphous, and nanocrystalline Co-rich cobalt phosphorus films. These nano-heterostructured films exhibit a unique non-collinear anisotropy-driven transverse exchange-spring behavior, investigated systematically under ambient conditions. The comprehensive functional analyses reveal that intricate interplay between in-plane (IP) anisotropy of amorphous phase and out-of-plane (OOP) anisotropy generating from a nanocrystalline structure compete with each other, while producing characteristic stripe domain structures to novel corrugated stripe domain shapes. The angle-dependent first-order reversal curve distributions demonstrate new insights into the magnetic reversal mechanisms, further confirming the non-exchange-spring and exchange-spring nature of the films depending on the prevalent interfacial exchange coupling. Formation of anisotropy-driven metastable-state due to competition between IP and OOP anisotropy at a particular OOP orientation has led the normal exchange-spring structures to a transverse exchange-spring structure. Micromagnetic simulations, in excellent agreement with experimental data, further elucidate the formation of characteristic stripe domain patterns and the influence of anisotropy on the magnetic properties. The innovative methodology and detailed functional analysis presented here offer significant understanding to the field of exchange-spring magnetic materials, including anisotropy-driven metastable states, demonstrating the potential for scalable and cost-effective fabrication of advanced nano-heterostructures with tailored magnetic properties.

A novel pipeline employing deep multi-attention channels network for the autonomous detection of metastasizing cells through fluorescence microscopy

Metastasis driven by cancer cell migration is the leading cause of cancer-related deaths. It involves significant changes in the organization of the cytoskeleton, which includes the actin microfilaments and the vimentin intermediate filaments. Understanding how these filament change cells from normal to invasive offers insights that can be used to improve cancer diagnosis and therapy. We have developed a computational, transparent, large-scale and imaging-based pipeline, that can distinguish between normal human cells and their isogenically matched, oncogenically transformed, invasive and metastasizing counterparts, based on the spatial organization of actin and vimentin filaments in the cell cytoplasm. Due to the intricacy of these subcellular structures, manual annotation is not trivial to automate. We used established deep learning methods and our new multi-attention channel architecture. To ensure a high level of interpretability of the network, which is crucial for the application area, we developed an interpretable global explainable approach correlating the weighted geometric mean of the total cell images and their local GradCam scores. The methods offer detailed, objective and measurable understanding of how different components of the cytoskeleton contribute to metastasis, insights that can be used for future development of novel diagnostic tools, such as a nanometer level, vimentin filament-based biomarker for digital pathology, and for new treatments that significantly can increase patient survival.

Assessing Atherosclerosis by Super-Resolution Imaging of HClO in Foam Cells Using a Ratiometric Fluorescent Probe.

Atherosclerosis (AS) is the leading cause of cardiovascular disease. Foam cells, with elevated lipid droplets (LDs) and HClO levels, are the main components of the atherosclerotic plaques that are characteristic of AS. Super-resolution imaging can be used to visualize the distribution of LDs in foam cells at the nanometer level, facilitating the identification of LDs and HClO. In the present study, we report the development of a ratiometric fluorescent probe, SFL-HClO, for super-resolution imaging of LDs and HClO. Super-resolution imaging with this probe revealed the precise structure of LDs at the suborganelle level. Moreover, the fluorescence behavior of SFL-HClO on the surface of LDs verified its excellent performance in detecting HClO in the foam cells. SFL-HClO can sequentially and specifically respond to LDs and HClO via "turn-on" and ratiometric signal output, respectively, thus contributing to precise imaging of foam cells. Importantly, we demonstrate that SFL-HClO can be used to report on upregulated HClO in atherosclerotic plaques in the aorta of AS mice, providing a suitable fluorescent tool for early atherosclerotic disease assessment.

Applications of nanoparticles in veterinary science: A promising technology for animal health

Objective: To revise and describe the importance of nanoparticles in veterinary sciences to improve the health and welfare of animals. Design/methodology/approach: The use of nanoparticles in veterinary sciences has the aim of using the unique properties of these structures at the nanometer level in order to solve medical and therapeutic problems in the field of veterinary medicine. Limitations on study/implications: Although the nanoparticles have great potential in veterinary medicine, they also have certain limitations that must be considered in their application, among which the following stand out: bioavailability, distribution, toxicity, immunologic interactions, and costs. Conclusions: The field of veterinary medicine is in constant evolution, which is why nanoparticles have become a cutting-age tool with transforming potential. Their application in the diagnosis, treatment and prevention of diseases offers ways to improve animal health and welfare. The continuous search for solid research and solutions to overcome the limitations will allow taking advantage of all the potential of these technologies.

Design of subsurface defect detection system based on two channels.

With the continuous improvement of quality requirements for optical components, the detection of subsurface defects in optical components has become a key technology. However, there is a problem with existing detection techniques, which is that they cannot simultaneously and independently detect subsurface defects at the micrometer and nanometer levels. This article analyzes the scattering field model of subsurface scratches and conducts simulation experiments on the relationship between scattering light intensity and system aperture. Based on the simulation results, a dual channel experimental system with adjustable spot size was designed to achieve automated measurement of subsurface defects. The narrow channel was used to detect micrometer-level subsurface defects and the wide channel was used to detect nanometer-level subsurface defects. The experimental results verified the correctness of the simulation experiment. In order to improve the sensitivity of the system, we designed an aperture based on the scattering field distribution of surface and subsurface defects, which is used to block the interference signal on the sample surface and improve the signal-to-noise ratio of the subsurface defect signal. The experimental results show that this aperture plays an important role, and the detection sensitivity of the system reaches 100 nm. We used four algorithms for data processing and found that the IQR algorithm is most suitable for this system. Finally, the detection results were compared under different spot sizes, and it was found that small spot sizes have better detection effects on nanoscale subsurface defects. In practice, the spot size can be dynamically adjusted according to the detection needs to achieve the optimal configuration of detection speed and sensitivity.

Cerium-based sorbent with 100% Ce(III) and high dispersion for enhanced phosphate removal from wastewater

Under the double pressure of eutrophication and phosphorus deficiency, there is an urgent need to remove excess phosphate from wastewater efficiently. Preparing cerium-based materials with excellent application potential inevitably leads to Ce(III) oxidation to Ce(IV), which reduces the phosphate adsorption effect and restricts its development. To solve this problem, we introduced a protective agent and successfully synthesised cerium-modified activated carbon sorbent containing 100 % Ce(III) during the synthesis process. Compared with CeAC without a protecting agent, CeAC-A contained more Ce(III) (100 %) and exchange groups (–OH accounted for 82.76 %), achieving an adsorption capacity of up to 448.57 mg-P/g Ce, improving of 4.3 times. At the same time, the size of the cerium active component was reduced from the micron level to the nanometer level, and the mass transfer rate was significantly improved. In addition, with the enormous specific surface area and well-developed pore structure of activated carbon, it showed high dispersibility, with P/Ce molar ratio as high as 2.03, which is 1.1–4.8 times higher than other cerium-based adsorbents. Phosphate was removed primarily through electrostatic attraction and ligand exchange. In treating municipal wastewater with complex water quality, CeAC-A showed strong selectivity, a wide range of adaptability and easy repeat regeneration, making it a promising adsorbent for phosphate uptake from wastewater.

Single Atom Engineering for Nanorobotics.

The fields of single atom engineering represent cutting-edge areas in nanotechnology and materials science, pushing the boundaries of how small we can go in engineering functional devices and materials. Nanorobots, or nanobots, are robotic systems scaled down to the nanometer level and designed to perform tasks at similarly small scales. Single atom engineering, on the other hand, involves manipulating individual atoms to create precise materials and devices with controlled properties and functionalities. By integrating single atom engineering into nanorobotics, we unlock the potential to enable the precise incorporation of multiple functionalities onto these minuscule machines with nanometer-level precision. In this perspective, we describe the nascent field of single atom engineering in nanorobotics.

Modification and strengthening of recycled Al-Mg-Si-based alloy upon continuous rheological extrusion (CRE) forming

With global warming and resource scarcity, it is crucial to devise a simple, energy-efficient, and low-emission recycling method for aluminum alloys. In the recycled Al-Mg-Si-based alloy, the coarse α-Al dendrites, the long plate-like β-Al5FeSi phase and bulk Mg2Si phase significantly impair the microstructure and mechanical properties of the alloys. In this works, combined the continuous rheological extrusion (CRE) technology with the addition of CRE Al-5.0Ti-1.0B (wt%) refiner, the recycled Al-Mg-Si-based alloy wires at extrusion ratios (ER) of 3, 5 and 7 were prepared. The refinement of Mg2Si and β-Al5FeSi phases and the grain refinement caused by the continuous dynamic recrystallization (CDRX) behavior in the CRE process were discussed. The strengthening mechanism of recycled Al-Mg-Si-based alloy was revealed. The results showed that, CRE technology and CRE Al-5.0Ti-1.0B (wt%) refiner can effectively refine Mg2Si and β-Al5FeSi phases, and even to the nanometer level. At the same time, the increase of ER and the addition of CRE Al-5.0Ti-1.0B (wt%) can promote the transformation of low angle grain boundaries (LAGBs) to high angle grain boundaries (HAGBs), thereby promoting the occurrence of CDRX. In addition, the increase of dislocations and nanophases caused by the increase of ER and the addition of CRE Al-5.0Ti-1.0B can effectively refine CDRX grains. When 0.2 wt% CRE Al-5.0Ti-1.0B (wt%) refiner was added at ER of 7, the ultimate tensile strength (UTS), yield strength (YS) and elongation (EL) of recycled Al-Mg-Si-based alloy were 259.40 MPa, 140.80 MPa and 18.20%, respectively. Grain boundary strengthening and dislocation strengthening were the primary mechanisms in enhancing the YS of the recycled Al-Mg-Si-based alloy.

Classical tribology and charge-energy evolution theory cooperate to determine nitrided ceramic coating/metal substrate interfacial friction

Who governs interfacial friction, classical tribology theory or charge-energy evolution theory? Since it was widely recognized that the origin of friction is interatomic forces, attention has gradually shifted towards the characteristics of local nanoscale interfaces. The new theory believes the charge density evolution during the friction process has more significant attributes in determining the interfacial friction. Here, we discuss the frictional failure mechanism at the ceramic/metal interface formed after the nitriding. The increase in N-doped interface friction is supported by classical tribology theory and corresponds to high interface adhesion work. On the other hand, the H-doped interface with the lowest adhesion work exhibits the highest friction. Unlike other interface systems (metallic bonding), the failure interface after H doping is an ionic bonding interface. Stronger ionic bonds are formed and broken in relative sliding, significantly increasing charge density. This emphasizes the crucial role of charge density in this process, aligning with the principles of the new theory. Nevertheless, the classical tribology theory can still be used when measuring interfacial friction of the interface with the same chemical bonds, and it may be more convincing than the new theory. Overall, these research results reveal the intrinsic origin of H/N's influence on TiN/Ti interfacial friction and provide new insights into understanding natural failures at the nanometer level.

Biocompatible Hybrid Graphenic Thin Coatings on Flexible Substrates through Matrix-Assisted Pulsed Laser Evaporation (MAPLE).

This work reports the production of biocompatible thin layers for biomedical applications based on a graphene-like material (GL), a graphene-related material (GRM) obtained from carbon black. GL was combined in a hybrid fashion with polydopamine (pDA), a mussel-inspired water-resistant wet adhesive bonding obtained by the oxidative polymerization of dopamine (DA), and polyvinyl pyrrolidinone (PVP), a nontoxic synthetic polymer with intrinsic adhesion properties, to obtain a tighter adhesion of the thin layer to the substrate (silicone slices). Matrix-assisted pulsed laser evaporation (MAPLE) was used to coat PDMS slices with thin films of GL-pDA and GL-PVP directly from their frozen suspensions in water. The results indicate that the relevant chemical-physical characteristics of both thin films (evidenced by FTIR and AFM) were maintained after MAPLE deposition and that the films exhibit uniformity also at the nanometric level. After deposition, the GL-pDA and GL-PVP films underwent a biological survey toward murine fibroblasts (NIH3T3), human keratinocytes (HaCAT), and human cervical adenocarcinoma epithelial-like (HeLa) cells to assess the feasibility of this approach. Results indicate that both the GL-pDA and GL-PVP films did not perturb the biological parameters evaluated, including cytoskeleton alterations. Both hybrid films enhanced the effects of GL on cellular vitality across all cell lines. Specifically, the GL-pDA film exhibited a more stable effect over time (up to 72 h), whereas the GL-PVP film behaved similarly to the GL film in NIH3T3 and HeLa cell lines after long-term exposure. These promising results make the GL-pDA and GL-PVP films potential candidates for the manufacture of coated flexible devices for biomedical applications.