Nanoencapsulation Techniques Research Articles

Temporary Nanoencapsulation of Human Intestinal Organoids Using Silk Ionomers.

Human intestinal organoids (HIOs) are vital for modeling intestinal development, disease, and therapeutic tissue regeneration. However, their susceptibility to stress, immunological attack, and environmental fluctuations limits their utility in research and therapeutic applications. This study evaluated the effectiveness of temporary silk protein-based layer-by-layer (LbL) nanoencapsulation technique to enhance the viability and functions of HIOs against common biomedical stressors, without compromising their native functions. Cell viability and differentiation capacity are assessed, finding that nanoencapsulation significantly improved HIO survival under the various environmental perturbations studied without compromising cellular functionality. Post-stress exposures, the encapsulated HIOs still successfully differentiated into essential intestinal cell types such as enterocytes, goblet cells, enteroendocrine cells, and Paneth cells. Moreover, the silk nanocoatings effectively protected against environmental stressors such as ultraviolet (UV) light exposure, protease degradation, antibody binding, and cytokine-induced inflammation. This nanoencapsulation technique shows promise for advancing HIO applications in disease modeling, drug testing, and potential transplantation therapies.

Protective Effects of Carotenoid-Loaded Nanostructured Lipid Carriers Against Ochratoxin-A-Induced Cytotoxicity.

Ochratoxin A (OTA) is a mycotoxin produced by Aspergillus ochraceous and various Penicillium species, which are known for contaminating agricultural products and posing significant health risks, which include immunotoxicity. This study aims to evaluate the potential of nanostructured lipid carriers (NLCs) loaded with a carotenoid-enriched extract from pumpkin peel (Cucurbita maxima L.) in mitigating the toxic effects of OTA. To address the poor bioavailability and instability of carotenoids, nanoencapsulation techniques were employed to enhance their delivery and efficacy. NLCs were formulated using hydrogenated sunflower oil, pumpkin oil, and soy lecithin using hot high-pressure homogenization. The in vitro study involved co-digesting OTA-contaminated bread with an NLC formulation and assessing the impact of the encapsulated carotenoid on OTA bioaccessibility, bioavailability, and cellular toxicity using Caco-2 and Jurkat T cells. Even though no significant influence was observed on the bioaccessibility and bioavailability of OTA, carotenoid-loaded NLCs exhibited cytoprotective effects by improving cell viability and mitigating OTA-induced toxicity in both Caco-2 and Jurkat T cells. Particularly, the flow cytometry analysis highlighted the ability of carotenoids to mitigate OTA-induced cellular damage by decreasing ROS production and limiting mitochondrial mass changes. The study suggests that the encapsulation of carotenoids in NLCs represents a promising strategy to enhance their protective effects against OTA toxicity, potentially offering a novel approach to food safety and public health protection. The study underscores the potential of nanotechnology in improving the bioavailability and efficacy of natural antioxidants to mitigate mycotoxin-induced damage.

Nanoemulsion: Composition, Preparation and its Application in the Food Industry

Nanotechnology deals with the application of atoms, molecules, or macromolecules with sizes ranging from 1 to 100 nm to create and utilize materials with distinct and novel characteristics. Nanotechnology has gained popularity over the past few decades and is now widely regarded as a revolutionary technology in the food industry. Various nanoencapsulation techniques have been spawned by the boom in interest in nanotechnology and its application in food products. One of the most exciting encapsulating and delivering methods in the food sector is nanoemulsion. A nanoemulsion is a mixture of two immiscible liquids and a surfactant that takes the form of tiny droplets less than 200 nm in size. Delivery methods based on nanoemulsions increase the bioavailability of the bioactive substances that are encapsulated and improve food stability. Since nanoemulsions are thermodynamically unstable, they need a certain amount of energy to create them. The preparation technique chosen determines the creation and permanence of nanoemulsions. This review provides an overview of nanoemulsion terminology and formulation; and also addresses various approaches for producing nanoemulsions, including both high- and low-energy techniques. In addition, the applications of nanoemulsions in the food industry.

Innovative Delivery Systems for Curcumin: Exploring Nanosized and Conventional Formulations.

Curcumin, a polyphenol with a rich history spanning two centuries, has emerged as a promising therapeutic agent targeting multiple signaling pathways and exhibiting cellular-level activities that contribute to its diverse health benefits. Extensive preclinical and clinical studies have demonstrated its ability to enhance the therapeutic potential of various bioactive compounds. While its reported therapeutic advantages are manifold, predominantly attributed to its antioxidant and anti-inflammatory properties, its efficacy is hindered by poor bioavailability stemming from inadequate absorption, rapid metabolism, and elimination. To address this challenge, nanodelivery systems have emerged as a promising approach, offering enhanced solubility, biocompatibility, and therapeutic effects for curcumin. We have analyzed the knowledge on curcumin nanoencapsulation and its synergistic effects with other compounds, extracted from electronic databases. We discuss the pharmacokinetic profile of curcumin, current advancements in nanoencapsulation techniques, and the combined effects of curcumin with other agents across various disorders. By unifying existing knowledge, this analysis intends to provide insights into the potential of nanoencapsulation technologies to overcome constraints associated with curcumin treatments, emphasizing the importance of combinatorial approaches in improving therapeutic efficacy. Finally, this compilation of study data aims to inform and inspire future research into encapsulating drugs with poor pharmacokinetic characteristics and investigating innovative drug combinations to improve bioavailability and therapeutic outcomes.

Developmental Formulation Principles of Food Preservatives by Nanoencapsulation-Fundamentals, Application, and Challenges.

The role of food additives is to preserve food by extending shelf life and limiting harmful microorganism proliferation. They prevent spoilage by enhancing the taste and safety of food by utilizing beneficial microorganisms and their antimicrobial metabolites. Current advances in food preservation and processing utilize green technology principles for green preservative formulation, enhancing nutrition and supplying essential micronutrients safely, while also improving quality, packaging, and food safety. Encapsulation is gaining attention for its potential to protect delicate materials from oxidative degradation and extend their shelf life, thereby ensuring optimal nutrient uptake. Nanoencapsulation of bioactive compounds has significantly improved the food, pharmaceutical, agriculture, and nutraceutical industries by protecting antioxidants, vitamins, minerals, and essential fatty acids by controlling release and ensuring delivery to specific sites in the human body. This emerging area is crucial for future industrial production, improving the sensory properties of foods like color, taste, and texture. Research on encapsulated bioactive compounds like bacteriocins, LAB, natamycin, polylysine, and bacteriophage is crucial for their potential antioxidant and antimicrobial activities in food applications and the food industry. This paper reviews nanomaterials used as food antimicrobial carriers, including nanoemulsions, nanoliposomes, nanoparticles, and nanofibers, to protect natural food antimicrobials from degradation and improve antimicrobial activity. This review discusses nanoencapsulation techniques for biopreservative agents like nisin, poly lysine, and natamycin, focusing on biologically-derived polymeric nanofibers, nanocarriers, nanoliposomes, and polymer-stabilized metallic nanoparticles. Nanomaterials, in general, improve the dispersibility, stability, and availability of bioactive substances, and this study discusses the controlled release of nanoencapsulated biopreservative agents.

Nanoencapsulation of Bixin and Norbixin in Sodium Alginate: Preparation, Characterization, and Release in Food Simulant

Aims: Bixin and norbixin are natural antioxidants used as pigments in the food industry, but their chemical structure makes them susceptible to environmental factors (light, oxygen, and temperature). Background: Nanoencapsulation techniques can improve the stability and solubility of these compounds in addition to reducing particle size which can increase surface:volume ratio and provide many attractive and unique properties to the nanoparticles. Objective: In this study, sodium alginate was used as wall material for the encapsulation of bixin and norbixin in different concentrations (1.25, 2.5, 5, and 7.5 g/g of biopolymer), by emulsification/internal gelation method. Methods: The emulsification/internal gelation method was used to elaborate bixin or norbixin-loaded nanospheres. The internal phase of the water-in-oil (W/O) emulsion was prepared with an aqueous solution of sodium alginate (1.5% w/v – 40 mL), 0.12 g of CaCO3, bixin or norbixin pigments, and mechanically stirred for 15 min at 700 rpm. Results: Nanospheres containing the highest concentration of both carotenoids showed better encapsulation efficiency, with 37.86% for bixin and 51.47% for norbixin, and these formulations were used for characterization analyses. The mean size of the nanospheres was 741.9 ± 41.0 nm, 622.9 ± 71.0 nm, and 589.5 ± 99.1 nm for control particles, bixin, and norbixin, respectively. The addition of both carotenoids resulted in particles with a yellow-red color, which demonstrates the encapsulation of natural antioxidants. The thermal analysis results may indicate an increase in the thermal stability of the pigments after encapsulation, in addition, the nanospheres exhibited the ability to scavenge the ABTS+ radical. Carotenoids release test in food simulant (95% ethanol) presented a rapid release in the first hours and maintenance of concentration for 10 days. Conclusion: Results showed that these nanospheres could be an alternative to the application of these carotenoid pigments in food matrices and food packaging.

Review on the Encapsulation, Microencapsulation, and Nano- Encapsulation: Synthesis and Applications in the Process Industry for Corrosion Inhibition

Background: Surfaces of materials often corrode and deteriorate due to environmental conditions. There are various widely used methods of reducing corrosion rates to increase the lifetime of materials and equipment. Recently, there has been a growth in the use of nanotechnology to protect metals against corrosion. The application of nano-encapsulation techniques in the process industry is one of the important eras of nanotechnology. This review paper focuses on encapsulation, microencapsulation, and nano-encapsulation methods, emphasizing nanoencapsulation applications as corrosion inhibitions in the process industry. Methods: Materials based on the self-healing mechanism were used in advanced applications such as structures, batteries, and coatings. These technologies may be studied in two ways: compounds with intrinsic self-healing properties and extrinsic self-healing materials with additives such as microcapsules filled with healing agents. Nano-coatings have advantages, like accelerated ground hardness, adhesive energy, long-time period and/or high-temperature corrosion resistance, tribological residence enhancement, etc. Nano-coatings can also be carried out in thinner and smoother layers, considering flexibility, accelerated performance, decreased fuel costs, and smaller carbon footprints, as well as occasional maintenance. The review of corrosion characteristics of polymeric nanocomposite material was discussed in this paper. Results: This review paper presents an updated overview summarizing the latest advances in the various micro/nanocarriers used for self-healing corrosion protective coatings. Conclusion: With this information, the investigators will be able to modify the structure of the inhibitor to get the necessary corrosion inhibition capabilities. The need for a physical examination is rising as a result.

Dual-targeted nano-encapsulation of neonatal porcine islet-like cell clusters with triiodothyronine-loaded bifunctional polymersomes

There is growing evidence that neonatal porcine islet-like cell clusters (NPCCs) isolated from piglets can be used to treat type 1 diabetes in humans. However, graft rejection is a common complication in humans owing to the prevalence of xenoantigens in porcine. Therefore, researchers have investigated various islet encapsulation techniques that could protect against these antigens. To this end, this study presents a robust nano-encapsulation method based on bifunctional polymersomes (PSomes), in which N-hydroxysuccinimide (NHS) and maleimide (Mal) groups conjugated to the PSomes terminal interact with the amine and thiol groups on the surface of NPCCs to induce dual targeting via two covalent bonds. The findings indicate that the ratio of NHS to Mal on PSomes is optimal for dual targeting. Moreover, triiodothyronine (T3) is known to promotes pancreatic islet maturation and differentiation of endocrine cells into beta cells. T3 encapsulated in PSomes is shown to increase the glucose sensitivity of NPCCs and enhance insulin secretion from NPCCs. Furthermore, improvements in the nano-encapsulation efficiency and insulin-secreting capability of NPCCs through dual targeting via dual-Psomes are demonstrated. In conclusion, the proposed nano-encapsulation technique could pave the way for significant advances in islet nano-encapsulation and the imprevement of NPCC immaturity via T3 release.

Innovative Food Safety Approaches and Nutraceuticals to Promote Children's Health on Future Outbreaks with the Reflection of COVID-19.

After the COVID-19 pandemic, innovative methods have emerged for the management of food safety, child nutrition has become more important than ever, and increasing attention has been paid to the consequences of COVID-19. For instance, since SARS-CoV-2 is an animal-based zoonotic virus, there is a changing trend in consumer preferences from conventional meat products to cultured meat and vegan supplementation. Due to the effects mentioned, this chapter provides strategic guidance on novel foods, food safety innovations, and novel health and safety procedures in public places such as restaurants or bars. There are also long-term health impacts on children in the aftermath of COVID-19. Since the risk of myopia is one of the important long-term effects to be considered, trending nutritional immunology approaches are presented to reduce emerging problems in child eye health. The enhancement of immune system remains problematic for many children considering that they cannot use the COVID-19 vaccine. Therefore, this chapter also emphasizes the importance of breastfeeding on the side effects of viral infections and new supplements, such as probiotic drops, to improve children's and babies' immune health. Additionally, efforts should be undertaken to improve nanoencapsulation techniques to prepare for future epidemics and pandemics. Nanomaterial-supported nutraceuticals, nanoencapsulation of functional ingredients or their nanoparticles, and nano-combination of phytochemicals, fatty acids, or probiotics should be investigated to improve the immunity of children. In this sense, detailed further research in this area needs to be adapted to innovative technologies for the treatment of infants and children against future zoonotic viruses.

Phenolic profile of micro- and nano-encapsulated olive leaf extract in biscuits during in vitro gastrointestinal digestion.

Olive leaf was characterized by a high content of phenols and flavonoids (oleuropein, luteolin, and their derivatives), presenting functional and health-related properties. The chemical instability of phenolics through technological processes and their degradation in the digestive system may negatively impact them, leading to lower absorption. This study evaluates the phenolic profile of micro- and nano-encapsulated olive leaf extract in biscuits during the INFOGEST static in vitro digestion, aiming to enhance stability and sensorial properties. Ultrasound-assisted extraction and chromatography characterized the extract, while spray drying (maltodextrin-glucose) and nano-encapsulation (maltodextrin, whey protein isolate, and arabic gum) techniques were used with specific solutions. Encapsulated formulations underwent microscopy (TEM, SEM) and encapsulation efficiency analysis. Micro- and nano-encapsulation improved biscuit functionality by enhancing phenolic stability during digestion. However, the highest concentration adversely affected sensory and textural parameters. These findings contribute to developing functional food products enriched with bioactive compounds, providing improved health benefits while maintaining sensory attributes.

Application of graphene and its derivatives in anticorrosion coatings: Research advances and future perspectives

BackgroundGraphene as a unique two-dimensional structure material has gained much attention due to its environmentally friendly properties, and competitive price. Good chemical stability and thermal stability make it stable in corrosive environments as well as high temperature conditions. Currently, there is a notable absence of a comprehensive and in-depth literature review on the use of graphene and its derivatives in protective coatings, with a focus on their protection mechanisms, dispersion techniques, orientation control, and functionalization. MethodThis article provides a conclusive review of the research on graphene-based composite anticorrosive coatings for metallic materials. It describes and discusses the mechanism, recent advances, and strategies. Focusing findings and achievements in the past decades. Further covers various state-of-the-art approaches to organic and inorganic modifications of graphene. Review the promotion of corrosion properties provided by the adaptation of functionalized graphene though grafting and nano-encapsulation techniques. Identifying the desirable characteristics and development direction for anticorrosion coatings with graphene introduced as filler. Significant findingsComprehensive and structural review of graphene and its derivatives in anticorrosion application was done from the aspect of its protection mechanism, dispersion methods, control over orientation, and functionalization. Some adverse properties and problems identified and the scope of further research on graphene in the anticorrosion application has been outlined.

Grape By-Products in Sustainable Cosmetics: Nanoencapsulation and Market Trends

The largest human organ, the skin, serves a variety of essential functions including protection, preservation of water and electrolytes, regulation of body temperature, and fat storage. Its maintenance and preservation are supported by cosmetic products, whose functions include cleaning, protection, and modulation. The market for these products is predicted to increase from 100.13 billion USD in 2021 to 145.82 billion USD in 2028. Recently, it has been suggested that grape by-products (totalling 14.5 million tons per year just in Europe) has a great potential to be used in the creation of new cosmetic products. In this regard, this article aims to provide a comprehensive overview of the current state of knowledge regarding the bioactive compounds in grape pomace, the advantages of applying them to the skin, and the main cosmetic products already on the market incorporating these bioactives. Most of these compounds are derived from the Vitis vinifera L. species, and exhibit several biological properties, such as antioxidant, antimicrobial, anti-inflammatory, inhibition of skin degrading enzymes, protection from UVA damage, increased cell viability, and skin whitening effect. On the other hand, nanoencapsulation techniques can provide a significant improvement in the stability of grape-derived bioactive compounds, in particular of resveratrol, and this issue is also addressed in a critical manner in this review.

Polyphenol-Loaded Polymeric Matrixes as Potential Biopharmaceuticals against Cancer

Polyphenols have attracted attention for their anti-inflammatory, antidiabetic, and anticancer properties. Due to the antioxidant and anti-inflammatory potential of these molecules, they are also proposed as a potential therapeutic tool to prevent complications of cancer and decrease the secondary effects of conventional chemotherapeutic drugs. Nonetheless, polyphenols such as flavonoids and phenolic acids have low bioavailability, as they are highly metabolized. Thus, administration strategies have been developed to enhance the anticancer properties of polyphenols. Most of these strategies involve different encapsulation techniques, such as nanoencapsulation, nanoemulsion, and the use of other polymeric matrixes. These techniques can increase the activity of these compounds after going through the gastrointestinal process and improve their solubility in an aqueous medium. This review comprises recent studies regarding encapsulation techniques to enhance the bioactivity of polyphenols against cancer and their current state in clinical studies. Overall, micro- and nanoencapsulation techniques with different polymers enhanced the anticancer properties of polyphenols by inhibiting tumor growth, modulating the expression of genes related to metastasis and angiogenesis, decreasing the expression of pro-inflammatory biomarkers.

Trends of Nanoencapsulation Strategy for Natural Compounds in the Food Industry

Nanotechnology is an emerging field in the food industry that will be important for future industrial production to address rising customer concerns and expectations for natural, nutritious, and healthful food items. People are increasingly motivated to purchase unprocessed food or even high-quality processed foods with minimum chemical additives, highlighting the need to investigate natural alternatives for commercial purposes. Natural compounds are becoming more popular among consumers since they are safer than synthetic chemical additions; however, their most functional compounds are sensitive to the adverse conditions of processing and the digestive tract, impairing their use in food matrices, and industrial-scale applications. Nowadays, nanoencapsulation of natural products can be the most suitable nanotechnology to improve stability, solubility, and bioavailability. The nanostructure can be incorporated into food during production, processing, packaging, and security. Despite the many studies on nanoencapsulation, there is still some misunderstanding about nanoencapsulation systems and preparation techniques. This review aims to categorize different nanoencapsulation techniques (chemical, physicochemical, and physicomechanical), highlight eco-friendly methods, and classify the nanoencapsulation systems as groups (polymer, lipidic and metallic). The current review summarizes recent data on the nanoencapsulation of natural compounds in the food industry that has been published since 2015 until now. Finally, this review presents the challenges and future perspectives on the nanoencapsulation of bioactive compounds in food science.

Nanoencapsulation of Cyanidin 3-O-Glucoside: Purpose, Technique, Bioavailability, and Stability.

The current growing attractiveness of natural dyes around the world is a consequence of the increasing rejection of synthetic dyes whose use is increasingly criticized. The great interest in natural pigments from herbal origin such as cyanidin 3-O-glucoside (C3G) is due to their biological properties and their health benefits. However, the chemical instability of C3G during processing and storage and its low bioavailability limits its food application. Nanoencapsulation technology using appropriate nanocarriers is revolutionizing the use of anthocyanin, including C3G. Owing to the chemical stability and functional benefits that this new nanotechnology provides to the latter, its industrial application is now extending to the pharmaceutical and cosmetic fields. This review focuses on the various nanoencapsulation techniques used and the chemical and biological benefits induced to C3G.

Drug-Eluting, Bioresorbable Cardiovascular Stents─Challenges and Perspectives.

Globally, the leading causes of natural death are attributed to coronary heart disease and type 1 and type 2 diabetes. High blood pressure levels, high cholesterol levels, smoking, and poor eating habits lead to the agglomeration of plaque in the arteries, reducing the blood flow. The implantation of devices used to unclog vessels, known as stents, sometimes results in a lack of irrigation due to the excessive proliferation of endothelial tissue within the blood vessels and is known as restenosis. The use of drug-eluting stents (DESs) to deliver antiproliferative drugs has led to the development of different encapsulation techniques. However, due to the potency of the drugs used in the initial stent designs, a chronic inflammatory reaction of the arterial wall known as thrombosis can cause a myocardial infarction (MI). One of the most promising drugs to reduce this risk is everolimus, which can be encapsulated in lipid systems for controlled release directly into the artery. This review aims to discuss the current status of stent design, fabrication, and functionalization. Variables such as the mechanical properties, metals and their alloys, drug encapsulation and controlled elution, and stent degradation are also addressed. Additionally, this review covers the use of polymeric surface coatings on stents and the recent advances in layer-by-layer coating and drug delivery. The advances in nanoencapsulation techniques such as liposomes and micro- and nanoemulsions and their functionalization in bioresorbable, drug-eluting stents are also highlighted.

Triplet Fusion Upconversion Nanocapsule Synthesis.

Triplet fusion upconversion (UC) allows for the generation of one high energy photon from two low energy input photons. This well-studied process has significant implications for producing high energy light beyond a material's surface. However, the deployment of UC materials has been stymied due to poor material solubility, high concentration requirements, and oxygen sensitivity, ultimately resulting in reduced light output. Toward this end, nanoencapsulation has been a popular motif to circumvent these challenges, but durability has remained elusive in organic solvents. Recently, a nanoencapsulation technique was engineered to tackle each of these challenges, whereupon an oleic acid nanodroplet containing upconversion materials was encapsulated with a silica shell. Ultimately, these nanocapsules (NCs) were durable enough to enable triplet fusion upconversion-facilitated volumetric three-dimensional (3D) printing. By encapsulating upconversion materials with silica and dispersing them in a 3D printing resin, photopatterning beyond the surface of the printing vat was made possible. Here, video protocols for the synthesis of upconversion NCs are presented for both small-scale and large-scale batches. The outlined protocols serve as a starting point for adapting this encapsulation scheme to multiple upconversion schemes for use in volumetric 3D printing applications.

Preparation of PLGA Nanoparticles by Milling Spongelike PLGA Microspheres

Currently, emulsification-templated nanoencapsulation techniques (e.g., nanoprecipitation) have been most frequently used to prepare poly-d,l-lactide-co-glycolide (PLGA) nanoparticles. This study aimed to explore a new top-down process to produce PLGA nanoparticles. The fundamental strategy was to prepare spongelike PLGA microspheres with a highly porous texture and then crush them into submicron-sized particles via wet milling. Therefore, an ethyl formate-based ammonolysis method was developed to encapsulate progesterone into porous PLGA microspheres. Compared to a conventional solvent evaporation process, the ammonolysis technique helped reduce the tendency of drug crystallization and improved drug encapsulation efficiency accordingly (solvent evaporation, 27.6 ± 4.6%; ammonolysis, 65.1 ± 1.7%). Wet milling was performed on the highly porous microspheres with a D50 of 64.8 μm under various milling conditions. The size of the grinding medium was the most crucial factor for our wet milling. Milling using smaller zirconium oxide beads (0.3~1 mm) was simply ineffective. However, when larger beads with diameters of 3 and 5 mm were used, our porous microspheres were ground into submicron-sized particles. The quality of the resultant PLGA nanoparticles was demonstrated by size distribution measurement and field emission scanning electron microscopy. The present top-down process that contrasts with conventional bottom-up approaches might find application in manufacturing drug-loaded PLGA nanoparticles.

Therapeutic Potential of Polyphenol and Nanoparticles Mediated Delivery in Periodontal Inflammation: A Review of Current Trends and Future Perspectives.

Periodontitis is an oral inflammatory process involving the periodontium, which is mainly caused by the invasion of periodontopathogenic microorganisms that results in gingival connective tissue and alveolar bone destruction. Metabolic products of the oral pathogens and the associated host immune and inflammatory responses triggered are responsible for the local tissue destruction. Numerous studies in the past decades have demonstrated that natural polyphenols are capable of modulating the host inflammatory responses by targeting multiple inflammatory components. The proposed mechanism by which polyphenolic compounds exert their great potential is by regulating the immune cell, proinflammatory cytokines synthesis and gene expression. However, due to its low absorption and bioavailability, the beneficial effects of these substances are very limited and it hampers their use as a therapeutic agent. To address these limitations, targeted delivery systems by nanoencapsulation techniques have been explored in recent years. Nanoencapsulation of polyphenolic compounds with different carriers is an efficient and promising approach to boost their bioavailability, increase the efficiency and reduce the degradability of natural polyphenols. In this review, we focus on the effects of different polyphenolic substances in periodontal inflammation and to explore the pharmaceutical significance of polyphenol-loaded nanoparticles in controlling periodontitis, which may be useful for further enhancement of their efficacy as therapeutic agents for periodontal disease.

Tea polyphenols-loaded nanocarriers: preparation technology and biological function.

Tea polyphenols (TP) have various biological functions including anti-oxidant, anti-bacterial, anti-apoptotic, anti-inflammatory and bioengineered repair properties. However, TP exhibit poor stability and bioavailability in the gastrointestinal tract. Nanoencapsulation techniques can be used to protect TP and to uphold their original characteristics during processing, storage and digestion, improve their physiochemical properties and enhance their health promoting effects. Nano-embedded TP show higher antioxidant, antibacterial and anticancer properties than TP, allowing TP to play a better role in bioengineering restoration after embedding. In this review, recent advances in nanoencapsulation of TP with biopolymeric nanocarriers (polysaccharides and proteins), lipid-based nanocarriers and innovative developments in preparation strategies were mainly discussed. Additionally, the strengthening biological functions of stability and bioavailability, antioxidant, antibacterial, anticancer activities and bioengineering repair properties activities after the nano-embedding of TP have been considered. Finally, further studies could be conducted for exploring the application of nanoencapsulated systems in food for industrial applications.