Nano-based Sensors Research Articles

Development, Optimization, and Clinical Relevance of Lactoferrin Delivery Systems: A Focus on Ocular Delivery.

Lactoferrin (Lf), a multifunctional protein found abundantly in secretions, including tears, plays a crucial role in ocular health through its antimicrobial, immunoregulatory, anti-inflammatory, and antioxidant activities. Advanced delivery systems are desirable to fully leverage its therapeutic potential in treating ocular diseases. The process of Lf quantification for diagnostic purposes underscores the importance of developing reliable, cost-effective detection methods, ranging from conventional techniques to advanced nano-based sensors. Despite the ease and non-invasiveness of topical administration for ocular surface diseases, challenges such as rapid drug elimination necessitate innovations, such as Lf-loaded contact lenses and biodegradable polymeric nanocapsules, to enhance drug stability and bioavailability. Furthermore, overcoming ocular barriers for the treatment of posterior segment disease calls for nano-formulations. The scope of this review is to underline the advancements in nanotechnology-based Lf delivery methods, emphasizing the pivotal role of multidisciplinary approaches and cross-field strategies in improving ocular drug delivery and achieving better therapeutic outcomes for a wide spectrum of eye conditions.

Recent advancements in Janus nanoparticle-based biosensing platforms.

Nanoparticles have aided in the development of nano-based sensors for diagnostic applications. However, use of nanoparticles in the development of sensing devices for multiple analyte detection is constrained due to their inability to detect several analytes with a single type of nanoparticle. The term “Janus particle” refers to micro or nanoscale particles that have been divided into sections or compartments, each of which has a distinct set of chemical or physical properties, producing multifunctional particles endowed with distinctive qualities. Furthermore, Janus particles have the ability to perform multiple functions within a single particle at the same time, with no interference from adjacent sections. This review focuses on the use of Janus particles in the fabrication of biosensors as well as in the investigation of various properties endowed by these Janus particles for their use as biosensors. It also discusses the various types of Janus particle-based biosensors that are currently available. Finally, the limitations of Janus particles in sensor technologies and their future scope have been discussed.

Smart nanomaterials as the foundation of a combination approach for efficient cancer theranostics

Despite numerous FDA-approved treatment and diagnostic approaches, cancer is one of the leading causes of death worldwide due to delayed diagnostics and the limited efficacy of treatments. The life quality of cancer patients is currently improving due to the development of nanotechnology-supported cancer diagnostics, detection, and nanomedicine for targeted drug delivery approaches investigated for cancer therapy. Considering these aspects, this comprehensive review presents the potential of smart nanomaterials (SMs) including carbon nanodots, metal-phenolic networks (MNPs), metal-organic frameworks (MOFs), quantum dots (QDs), carbon nanotubes (CNTs), metal/metal oxide nanoparticles, and silica nanoparticles, as well as polymeric nanoparticles, liposomes, amino acids, proteins, natural polysaccharides, and solid lipid nanoparticles, to manage cancer efficiently in a personalized manner. The outcomes of studies published in the last five years are critically analyzed in this review along with challenges and prospects in terms of a wide range of imaging technologies and therapeutic approaches, such as radiation responsive theranostics, magnetic responsive theranostics, drug delivery, photothermal/photodynamic therapy, and gene therapy. We believe that a novel multidisciplinary and combinational approach can be developed for early-stage cancer diagnostics and treat the diseases with efficacy using nano-based sensors, imaging agents, and pharmacologically relevant cargos. To promote research from the laboratory to clinical application, this review discusses viewpoints based on the tremendous potential of new and developing nanomaterials as a potential platform for cancer theranostics.

The dichotomy of nanotechnology as the cutting edge of agriculture: Nano-farming as an asset versus nanotoxicity

The unprecedented setbacks and environmental complications, faced by global agro-farming industry, have led to the advent of nanotechnology in agriculture, which has been recognized as a novel and innovative approach in development of sustainable farming practices. The agricultural regimen is the “head honcho” of the world, however presently certain approaches have been imposing grave danger to the environment and human civilization. The nano-farming paradigm has successfully elevated the growth and development of plants, parallel to the production, quality, germination/transpiration index, photosynthetic machinery, genetic progression, and so on. This has optimized the traditional farming into precision farming, utilising nano-based sensors and nanobionics, smart delivery tools, nanotech facets in plant disease management, nanofertilizers, enhancement of plant adaptive potential to external stress, role in bioenergy conservation and so on. These applications portray nanorevolution as “the big cheese” of global agriculture, mitigating the bottlenecks of conventional practices. Besides the applications of nanotechnology, the review identifies the limitations, like possible harmful impact on environment, mankind and plants, as the “Achilles heel” in agro-industry, aiming to establish its defined role in agriculture, while simultaneously considering the risks, in order to resolve them, thus abiding by “technology-yes, but safety-must”. The authors aim to provide a significant opportunity to the nanotech researchers, Botanists and environmentalists, to promote judicial use of nanoparticles and establish a secure and safe environment.

Versatility of Reverse Micelles: From Biomimetic Models to Nano (Bio)Sensor Design

This paper presents an overview of the principal structural and dynamics characteristics of reverse micelles (RMs) in order to highlight their structural flexibility and versatility, along with the possibility to modulate their parameters in a controlled manner. The multifunctionality in a large range of different scientific fields is exemplified in two distinct directions: a theoretical model for mimicry of the biological microenvironment and practical application in the field of nanotechnology and nano-based sensors. RMs represent a convenient experimental approach that limits the drawbacks of the conventionally biological studies in vitro, while the particular structure confers them the status of simplified mimics of cells by reproducing a complex supramolecular organization in an artificial system. The biological relevance of RMs is discussed in some particular cases referring to confinement and a crowded environment, as well as the molecular dynamics of water and a cell membrane structure. The use of RMs in a range of applications seems to be more promising due to their structural and compositional flexibility, high efficiency, and selectivity. Advances in nanotechnology are based on developing new methods of nanomaterial synthesis and deposition. This review highlights the advantages of using RMs in the synthesis of nanoparticles with specific properties and in nano (bio)sensor design.

Nanomedicine for the SARS-CoV-2: State-of-the-Art and Future Prospects.

The newly emerged ribonucleic acid (RNA) enveloped human beta-coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection caused the COVID-19 pandemic, severely affects the respiratory system, and may lead to death. Lacking effective diagnostics and therapies made this pandemic challenging to manage since the SARS-CoV-2 transmits via human-to-human, enters via ACE2 and TMPSSR2 receptors, and damages organs rich in host cells, spreads via symptomatic carriers and is prominent in an immune-compromised population. New SARS-CoV-2 informatics (structure, strains, like-cycles, functional sites) motivated bio-pharma experts to investigate novel therapeutic agents that act to recognize, inhibit, and knockdown combinations of drugs, vaccines, and antibodies, have been optimized to manage COVID-19. However, successful targeted delivery of these agents to avoid off-targeting and unnecessary drug ingestion is very challenging. To overcome these obstacles, this mini-review projects nanomedicine technology, a pharmacologically relevant cargo of size within 10 to 200 nm, for site-specific delivery of a therapeutic agent to recognize and eradicate the SARS-CoV-2, and improving the human immune system. Such combinational therapy based on compartmentalization controls the delivery and releases of a drug optimized based on patient genomic profile and medical history. Nanotechnology could help combat COVID-19 via various methods such as avoiding viral contamination and spraying by developing personal protective equipment (PPE) to increase the protection of healthcare workers and produce effective antiviral disinfectants surface coatings capable of inactivating and preventing the virus from spreading. To quickly recognize the infection or immunological response, design highly accurate and sensitive nano-based sensors. Development of new drugs with improved activity, reduced toxicity, and sustained release to the lungs, as well as tissue targets; and development of nano-based immunizations to improve humoral and cellular immune responses. The desired and controlled features of suggested personalized therapeutics, nanomedicine, is a potential therapy to manage COVID-19 successfully. The state-of-the-art nanomedicine, challenges, and prospects of nanomedicine are carefully and critically discussed in this report, which may serve as a key platform for scholars to investigate the role of nanomedicine for higher efficacy to manage the COVID-19 pandemic.

Development of Selenium Nanoparticle Based Agriculture Sensor for Heavy Metal Toxicity Detection

The presence of heavy metals in increased concentrations in the environment has become a global environmental concern. This rapid increase in heavy metals in the environment is attributed to enhanced industrial and mining activities. Metal ions possess a lengthy half-life and property to bioaccumulate, are non-biodegradable and, thus, are a threat to the human health. A number of conventional spectroscopic and chromatographic techniques are being used for the detection of heavy metals, but these suffer from various limitations. Nano-based sensors have emerged as potential candidates for the sensitive and selective detection of heavy metals. Thus, the present study was focused on the synthesis of selenium nanoparticles (SeNPs) by using selenite-reducing bacteria in the development of a heavy metal toxicity biosensor. During the biosynthesis of selenium nanoparticles, supernatants of the overnight-grown culture were treated with Na2SeO32− and incubated for 24 h at 37 °C. The as-synthesized nanoparticles were characterized by UV–Vis spectroscopy, X-ray diffraction (XRD), Fourier transform infrared (FTIR) and transmission electron microscopy (TEM) analyses. XRD and TEM results confirmed the formation of SeNPs in sizes ranging from 35 to 40 nm, with face-centered cubic (FCC) structures. The bioreduction process and validation of the formation of SeNPs was further confirmed by FTIR studies. The reduction in the biosynthesis of SeNPs using bacterial metabolite due to heavy metal cytotoxicity was analyzed by the colorimetric bioassay (SE Assay). The inhibition of selenite reduction and loss of red color in the presence of heavy metals may serve as a biosensor for heavy metal toxicity analysis. Thus, this biosensor development is aimed at improving the sensitivity and specificity of analytic detection.

Advances in Screening, Detection and Enumeration of Escherichia coli Using Nanotechnology-Based Methods: A Review.

Amongst the various microorganisms found as contaminants in food and water samples, Escherichia coli (E. coli) is one of the most predominant bacteria observed. The presence of the various strains of E. coli, pathogenic or non-pathogenic, in either food or water samples serves as an indicator of faecal contamination and helps to determine the hygienic condition, potability and safety of a given water or food sample for consumption. Although several conventional microbial analytical methods and the more advanced biochemical and molecular biology based methods exist for detection of E. coli, these are laden with several limitations. Nanotechnology offers cheaper, more reliable, quicker and more sensitive detection platforms for screening and/or enumerating the load of E. coli in a given sample. The present review outlines in brief the conventional methods available for E. coli detection enlisting the drawbacks of the same. Against this background, nanotechnology-based sensing systems developed for detection and capturing of E. coli so far have been highlighted. The most commonly developed nanosensors employ gold or silver nanoparticles with magnetic nanobeads also being currently employed for sensor development. Further, the evolution of green nanoparticle technology and its application in E. coli sensing has been explored with various examples of carbon dots, graphitic carbon nitride, glycopolymers and rice husk ash being cited. Furthermore, the development of nanosensors for E. coli using bimetallic nanoparticles has also been reviewed. These nanobased sensors have proved to exhibit high sensitivities and low detection limits for E. coli. However, approaches to enhance the sensitivity to detection even further lower limits and provide nanosensors as multifunctionalized detection tools for multiple pathogenic strains are underway.

Identification of catecholamine neurotransmitters using fluorescence sensor array

A nano-based sensor array has been developed for identification and discrimination of catecholamine neurotransmitters based on optical properties of their oxidation products under alkaline conditions. To produce distinct fluorescence response patterns for individual catecholamine, quenching of thioglycolic acid functionalized cadmium telluride (CdTe) quantum dots, by oxidation products, were employed along with the variation of fluorescence spectra of oxidation products. The spectral changes were analyzed with hierarchical cluster analysis (HCA) and principal component analysis (PCA) to identify catecholamine patterns. The proposed sensor could efficiently discriminate the individual catecholamine (i.e., dopamine, norepinephrine, and l-DOPA) and their mixtures in the concentration range of 0.25–30 μmol L−1. Finally, we found that the sensor had capability to identify the various catecholamines in urine sample.